Microbiota metabolites that shape host physiology

ABSTRACT

Methods of identifying test compounds or mixtures of test compounds from microbiota that bind to a fusion protein, such as a G-protein coupled receptor, are described. Also described are methods for high throughput screening of microbiota metabolites that are capable of activating G-protein coupled receptors.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application claims priority to U.S. Provisional Application No. 62/777,480, filed Dec. 10, 2018, which application is herein incorporated by reference in its entirety.

STATEMENT AS TO FEDERALLY SPONSORED RESEARCH

This invention was made with government support under grant AI123477 awarded by National Institutes of Health. The government has certain rights in the invention.

BACKGROUND

The microbiota metabolome results from a complex web of interactions between multiple microbial species and strains, environmental inputs (e.g., diet), and host factors. Advanced metabolomic, metagenomic and functional genomic approaches have revealed that the human microbiota can produce tens of thousands of unique small molecules (Donia and Fischbach, 2015; Milshteyn et al., 2018; Nicholson et al., 2012). However, the sheer magnitude and complexity of the gut microbiota metabolome can create significant challenges for dissecting how intra- and inter-species microbial chemistries affect host physiology (Donia and Fischbach, 2015; Fischbach, 2018). Understanding of the potential effects of the microbiota metabolome on the human host remains in its infancy.

MAOIs were the first FDA approved antidepressants (Ramachandraih, 2011); however, they have fallen out of favor due to potentially fatal dietary and drug-drug interactions (Fiedorowicz, 2004). Nonetheless, MAOIs remain an important treatment option for patients with refractory depression and other psychiatric disorders (Fiedorowicz, 2004), as well as Parkinson's disease (Riederer and Laux, 2011).

Histamine is generated via decarboxylation of L-His (Tannase, 1985). Histamine is involved in inflammatory responses and can regulate gut physiological function.

SUMMARY OF THE INVENTION

In one aspect is provided a method for determining if a test compound modulates an activity of a first protein in vivo, comprising

(a) contacting said compound with a cell comprising (i) a first nucleic acid molecule which encodes a first fusion protein comprising a first protein, a cleavage site for a protease, and a protein which activates transcription of a reporter gene in said cell, (ii) a second nucleic acid molecule which encodes a second fusion protein comprising a second protein which interacts with the first protein upon activation of the first protein and a protease or a fragment thereof capable of cleaving the protease cleavage site within the first fusion protein, and (iii) a third nucleic acid molecule which comprises a reporter gene, wherein said reporter gene is a barcode sequence operably linked to an element responsive to the protein which activates its transcription, and

(b) determining the level of transcription of said barcode sequence.

In some embodiments, the first nucleic acid molecule, the second nucleic acid molecule and the third nucleic acid molecule are clonally expressed to enable linkage of a specific receptor to an individual barcode. In some embodiments, the first nucleic acid molecule, the second nucleic acid molecule and the third nucleic acid molecule are clonally expressed through co-transfection. In some embodiments, the first nucleic acid molecule, the second nucleic acid molecule and the third nucleic acid molecule are clonally expressed through stable expression. In some embodiments, the barcode sequence comprises from 4 to 50 bases.

In some embodiments, the method further comprises

(c) concluding that the test compound activates the first protein if the level of transcription of said barcode sequence is increased in the presence of the test compound as compared to a control where no test compound is present.

In some embodiments, the method further comprises

(c) concluding that the test compound activates the first protein if the level of transcription of said barcode sequence is increased in the presence of the test compound as compared to an untreated cell.

In some embodiments, the method further comprises

(c) concluding that the test compound activates the first protein if the level of transcription of said barcode sequence is increased in the presence of the test compound as compared to a cell treated with a single dose of an agonist of the first protein in combination with an antagonist of the first protein.

In some embodiments, the first protein is a transmembrane protein. In some embodiments, the first protein is a G-protein coupled receptor (GPCR). In some embodiments, the GPCR is a non-olfactory GPCR. In some embodiments, the GPCR is an orphan GPCR. In some embodiments, the GPCR is a human GPCR.

In various embodiments, the protein which activates transcription of the reporter gene in said cell is tTA, a cas9 fusion protein, gal4/VP16, the estrogen receptor, the androgen receptor, the mineralocorticoid receptor, or the glucocorticoid receptor. In some embodiments, the cas9 fusion protein is cas9-vp64.

In various embodiments, the protein which activates transcription of the reporter gene in said cell is tTA. In some embodiments, the second protein which interacts with the first protein upon activation of the first protein is β-arrestin, a G-protein receptor kinase (GRK), or G-alpha. In some embodiments, the second protein is β-arrestin. In some embodiments, the protease is a Tobacco Etch Virus nuclear inclusion A protease (TEV protease). In some embodiments, the cell is a non-adherent mammalian cell. In some embodiments, the cell is Expi 293 T cell, Jurkat, Hela T4, raji, ramos, cho-s, or thp1 cells.

In various embodiments, the level of transcription of said barcode sequence is determined by sequencing of cDNA. In some embodiments, the cDNA is produced by reverse transcription of mRNA isolated from the cell using a polydT primer. In some embodiments, the test compound is a metabolite produced by a bacterial taxon contained within a microbiota of a subject. In some embodiments, the test compound is a metabolite produced by a bacterial strain contained within a microbiota of a subject. In some embodiments, the microbiota is a gastrointestinal (GI) microbiota. In some embodiments, the bacterial strain is clonally arrayed and cultured in vitro. In some embodiments, the method further comprises identifying the bacterial strain. In some embodiments, the bacterial strain is identified using 16S rRNA gene sequencing or whole genome sequencing. In some embodiments, the subject is human.

In some embodiments, the method is conducted in a high-throughput format, comprising:

(i) transfecting or transducing a plurality of cells separated into individual wells of a multi-well plate with the first, second and third nucleic acid molecules so that each transfected or transduced cell has a specific combination of the first protein and the barcode sequence; (ii) mixing the transfected cells; (iii) rearraying mixed cells into individual wells of a multi-well plate; (iv) exposing the rearrayed cell mixtures to one or more test compounds; (v) sequencing the barcodes, and (vi) determining which barcode sequences are increased in the presence of the test compound(s) as compared to a control where no test compound(s) is present.

In some embodiments, the method is conducted in a high-throughput format, comprising:

(i) transfecting or transducing a plurality of cells that stably express the second nucleic acid molecule (Barr-TEV) with the first and third nucleic acid molecules (the receptor and barcode);

(ii) mixing the transfected cells;

(iii) rearraying mixed cells into individual wells of a multi-well plate;

(iv) exposing the rearrayed cell mixtures to one or more test compounds;

(v) sequencing the barcodes, and

(vi) determining which barcode sequences are increased in the presence of the test compound(s) as compared to a control where no test compound(s) is present.

In some embodiments, the first nucleic acid molecule encodes a GPCR, the second nucleic acid molecule encodes Barr-TEV, and the third nucleic acid molecule comprises a barcode.

In another aspect is provided a method for high-throughput screening of microbiota metabolites capable of modulating the activity of a plurality of G-protein coupled receptors (GPCRs), the method comprising:

a) providing a plurality of non-adherent mammalian cells, wherein each cell comprises (i) a first nucleic acid molecule encoding a first fusion protein comprising a GPCR linked to the transcription factor tTA via a cleavage site for Tobacco Etch Virus nuclear inclusion A protease (TEV protease), (ii) a second nucleic acid molecule encoding a second fusion protein comprising 3-arrestin and TEV protease configured to cleave the TEV protease site on the first fusion protein, and (iii) a third nucleic acid molecule comprising a barcode sequence operably linked to a promoter specifically activated by the tTA transcription factor, wherein each barcode sequence is thus specifically linked to an individual GPCR;

b) contacting the plurality of cells with one or more microbiota metabolites or one or more compounds;

c) sequencing the barcodes; and

d) determining which barcode sequence transcription levels are increased or decreased in the presence of the metabolites as compared to a control where no metabolites are present.

In some embodiments, the GPCR is a non-olfactory GPCR. In some embodiments, the GPCR is an orphan GPCR. In some embodiments, the GPCR is a human GPCR. In some embodiments, the cell is Expi 293 T cell. In some embodiments, the level of transcription of said barcode sequence is determined by sequencing of cDNA. In some embodiments, the cDNA is produced by reverse transcription of RNA isolated from the cell. In some embodiments, the microbiota is a gastrointestinal (GI) microbiota. In some embodiments, the method further comprises identifying a bacterial strain producing the specific metabolite which activates the specific GPCR. In some embodiments, the bacterial strain is identified using 16S rRNA sequencing.

In another aspect is provided a method of preventing or treating monoamine oxidase inhibitor (MAOI)-induced toxicity in a subject, the method comprising administering an antibiotic effective to target a bacterial strain comprising a nucleic acid sequence encoding a phenethylamine production gene. In yet another aspect is provided a method of preventing or treating MAOI-induced toxicity in a subject comprising administering an antibiotic effective to target Morganella spp.

In another aspect is provided a method of treating a disease or condition caused by decreased MAO activity in a subject, the method comprising administering an antibiotic effective to target an organism comprising a nucleic acid sequence encoding a phenethylamine production gene.

In some embodiments, the organism is a bacterial strain. In some embodiments of the above aspects, the bacterial strain produces phenethylamine. In some embodiments, the bacterial strain secretes phenethylamine. In some embodiments, the disease is Brunner syndrome. In some embodiments, the condition is autism or anti-social behavior. In some embodiments, the subject expresses a MAOA-L variant. In some embodiments, the antibiotic is effective to target Morganella morganii. In some embodiments, the antibiotic is cefepime, piperacillin, tazobactam, ceftazidime, cefotaxime, ceftibuten, meropenem, doripenem, ertapenem, a fluoroquinolone, or an aminoglycoside.

In another aspect is provided a method of treating depression in a subject comprising administering a bacterial strain comprising a nucleic acid sequence encoding a phenethylamine production gene. In some embodiments, the method further comprises administering an anti-depressant to the subject. In some embodiments, the anti-depressant is an MAOI. In some embodiments, the bacterial strain produces phenethylamine.

In another aspect is provided a method for evaluating potential toxicity of a monoamine oxidase inhibitor (MAOI) in a subject, the method comprising

a) obtaining a gastrointestinal microbiota sample from the subject, and b) assaying the sample for the presence of a bacterial taxon or bacterial strain comprising a phenethylamine production gene.

In some embodiments, the amount of a phenethylamine producing enzyme exceeds a defined fraction of the enzymes produced by the microbiota. In some embodiments, the gastrointestinal microbiota sample is a fecal sample.

In another aspect is provided a method for evaluating potential efficacy of an MAOI in a subject, the method comprising

a) taking a fecal sample or a sample from the gastrointestinal tract of the subject, and

b) assaying for the presence of a bacterial strain comprising a nucleic acid sequence encoding a phenethylamine production gene.

In some embodiments, the method further comprises assaying for the amount of the bacterial strain present in the gastrointestinal tract. In some embodiments, the method further comprises treating the subject with an MAOI. In some embodiments, the method further comprises adjusting or determining the dosage of the MAOI based on the presence and/or amount of the bacterial strain present. In some embodiments, the bacterial strain is a bacterium of Morganella spp. In some embodiments, the bacterial strain is Morganella morganii.

In another aspect is provided a method of preventing or treating histamine-induced gastrointestinal disease in a subject, the method comprising administering one or more antibiotics effective to target a bacterial strain comprising a nucleic acid sequence encoding a histamine production gene. In some embodiments, the histamine production gene is a histidine decarboxylase. In some embodiments, the abundance of the histidine decarboxylase is higher in a patient with Crohn's disease as compared to a subject without inflammatory bowel disease. In some embodiments, the abundance of the histidine decarboxylase is higher in a patient with ulcerative colitis as compared to a subject without inflammatory bowel disease. In some embodiments, the bacterial strain is L. reuteri or a bacterium of Morganella spp. In some embodiments, the gastrointestinal disease is diarrhea.

In another aspect is provided a method of preventing or treating an allergy in a subject, the method comprising administering one or more antibiotics effective to target a bacterial strain comprising a nucleic acid sequence encoding a histamine production gene. In some embodiments, the bacterial strain is L. reuteri or a bacterium of Morganella spp.

In another aspect is provided a method of preventing or treating asthma in a subject, the method comprising administering one or more antibiotics effective to target a bacterial strain comprising a nucleic acid sequence encoding a histamine production gene. In some embodiments, the bacterial strain is L. reuteri or a bacterium of Morganella spp. In some embodiments, the antibiotic is cefepime, piperacillin, tazobactam, ceftazidime, cefotaxime, ceftibuten, meropenem, doripenem, ertapenem, a fluoroquinolone, or an aminoglycoside. In some embodiments, the method further comprises administering a histidine decarboxylase inhibitor. In some embodiments, the histidine decarboxylase inhibitor is rugosin D, rugosin A methyl ester, tellimagrandin II, rugosin A, pinocembrin, α-fluoromethylhistidine, brocresine, lecanoric acid, 2-hydroxy-5-carbomethoxybenzyloxyamine, and aminooxy analogs of histamine.

In another aspect is provided a method for evaluating potential effectiveness of an antibiotic to treat a gastrointestinal condition or disease, allergy or asthma in a subject, the method comprising

a) taking a fecal sample or a sample from the gastrointestinal tract of the subject, and

b) assaying for the presence of a bacterial strain comprising a nucleic acid sequence encoding a histamine production gene.

In some embodiments, the method further comprises assaying for the amount of the bacterial strain present in the gastrointestinal tract. In some embodiments, the method further comprises treating the subject with one or more antibiotics. In some embodiments, the antibiotic is cefepime, piperacillin, tazobactam, ceftazidime, cefotaxime, ceftibuten, meropenem, doripenem, ertapenem, a fluoroquinolone, or an aminoglycoside. In some embodiments, the method further comprises adjusting or determining the dosage of the antibiotic based on the presence and/or amount of the bacterial strain present. In some embodiments, the bacterial strain is a bacterium of Morganella spp. In some embodiments, the bacterial strain is Morganella morganii.

In another aspect is provided a method of preventing or treating a disease or condition resulting from production of phenethylamine, the method comprising administering one or more antibiotics effective to target a bacterial strain producing L-phenylalanine. In yet another aspect is provided a method of preventing or treating phenylketonuria (PKU) in a subject, the method comprising administering one or more antibiotics effective to target a bacterial strain producing L-phenylalanine.

In another aspect is provided a method of preventing or treating a disease or condition resulting from production of phenethylamine, the method comprising administering a Shikimate pathway inhibitor or an antagonist of aromatic L-amino acid decarboxylase. In yet another aspect is provided a method of preventing or treating phenylketonuria (PKU) in a subject, the method comprising administering a Shikimate pathway inhibitor or an antagonist of aromatic L-amino acid decarboxylase.

In some embodiments, the antagonist is carbidopa, benserazide, methyldopa, 3′,4′,5,7-Tetrahydroxy-8-methoxyisoflavone (DFMD), 3-hydroxybenzylhydrazine, 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2), or α-difluoromethyl DOPA. In some embodiments, the bacterial strain is B. thetaiotaomicron. In some embodiments, the bacterial strain is a strain C34 of B. thetaiotaomicron.

In some embodiments, the antibiotic is ampicillin, clavulanate, tazobactam, a cephamycin, ticarcillin, piperacillin, a cephalosporin, a carbapenem, clindamycin, lincomycin, chloramphenicol, a nitroimidazole, a fluoroquinolone.

In some embodiments, the antibiotic comprises (a) a combination of ampicillin and sulbactam, (b) a combination of ticarcillin and clavulanate, or (c) a combination of piperacillin and tazobactam.

In various embodiments of the above, the method further comprises administering a probiotic composition. In some embodiments, the probiotic composition is administered after antibiotic administration.

In some embodiments, the administration of the antibiotic and the administration of the probiotic composition are repeated in a cycle.

In another aspect is provided a kit for evaluating potential toxicity of an MAOI in a subject, the kit comprising a nucleic acid, antibody, or other reagent capable of binding specifically to a nucleotide or protein expressed by a bacterial strain, wherein the bacterial strain comprises a nucleic acid sequence encoding a phenethylamine production gene. In some embodiments, the bacterial strain is a bacterium of the Morganella spp. In some embodiments, the bacterial strain is Morganella morganii.

In another aspect is provided a kit for evaluating potential effectiveness of an antibiotic to treat a gastrointestinal condition or disease, allergy or asthma in a subject, the kit comprising a nucleic acid, antibody, or other reagent capable of binding specifically to a nucleotide or protein expressed by a bacterial strain, wherein the bacterial strain comprises a nucleic acid sequence encoding a histamine production gene. In some embodiments, the bacterial strain is L. reuteri or a bacterium of Morganella spp.

BRIEF DESCRIPTION OF THE DRAWINGS

FIGS. 1A-1C are diagrams showing PRESTO-Salsa, a novel high-throughput technology to screen metabolite mixtures against hundreds of GPCRs in parallel in a single tube. FIG. 1A is a diagram depicting the PRESTO-Tango technology. Briefly, upon ligand binding to a GPCR, recruitment of a Beta-arrestin-Tobacco Etch Virus nuclear inclusion endopeptidase (Barr-TEV) fusion triggers release of the transcription factor tTA and production of luciferase. FIG. 1B shows modification of the PRESTO-Tango assay to link GPCR activation to transcription of a specific nucleic acid barcode rather than production of luciferase. FIG. 1C shows that by pooling cells that clonally express specific GPCR-barcode pairs, Presto-Salsa enables simultaneous evaluation of 300+ GPCRs in a single tube via NGS. In this example, the in vitro cultured bacterial strain is producing a ligand for ‘GPCR2’ which leads to increased transcription of the corresponding GPCR2 barcode.

FIG. 2 is a heatmap showing that PRESTO-Salsa accurately detects GPCR activation via barcode counting in pooled libraries. Activation of pooled Expi293 cells expressing GPCR-barcode pairs were stimulated with known GPCR ligands and activation was determined via next-generation sequencing and barcode counting.

FIG. 3 is a diagram showing the PRESTO-Tango experiment performed on 144 unique human gut bacteria spanning five phyla, nine classes, eleven orders, and twenty families; these bacteria were isolated from fecal samples from 11 inflammatory bowel disease patients via high-throughput anaerobic culturomics and identified via massively barcoded 16S rRNA gene sequencing. Each isolate was grown in monoculture in a medium specialized for the cultivation of human gut microbes (gut microbiota medium). Supernatants from individual bacterial monocultures were screened against the near-complete non-olfactory GPCRome (314 conventional GPCRs) using the high-throughput assay Parallel Receptor-ome Expression and Screening via Transcriptional Output-Tango (PRESTO-Tango).

FIG. 4 is a chart showing GPCR activation by metabolomes from 144 bacterial strains isolated from the human gut microbiota. Data is displayed on a hierarchical tree of GPCRs organized by class, ligand type, and receptor family. Shading intensity represents the maximum magnitude of activation (log 2) over background across the entire data set, i.e., the maximum activation of a given GPCR by any microbial metabolome in our collection.

FIG. 5A is a diagram showing activation of aminergic GPCRs by 144 human gut bacteria as measured by PRESTO-Tango. Screening results are displayed on a phylogenetic tree of aminergic GPCRs. The 5-HT receptors are 5HT1A, 5HT1B, 5HT1D, 5HT1E, 5HT1F, 5HT7R, 5HT5A, 5HT2B, 5HT2C, 5HT2A, and 5HT6R. The acetylcholine receptors are ACM2, ACM4, ACM3, ACM1, and ACM5. The adernoceptors are ADRB3, ADRB2, ADRB1, ADA2A, ADA2C, ADA2B, ADA1D, ADA1A, and ADA1B. The dopamine receptors are DRD1, DRD5, DRD3, DRD2, and DRD4. The histamine receptors are HRH2, HRH1, HRH3 AND HRH4. The trace amine receptor is TAAR1. Shading intensity represents magnitude of activation over media alone and radii of the circles represents the number of bacteria that activated a given GPCR by more than two-fold over media alone.

FIG. 5B is a heatmap depicting the activation of aminergic GPCRs by metabolites from 144 human gut bacteria as measured by PRESTO-Tango. Fold induction over stimulation with bacterial media alone is depicted on a log 2 scale.

FIG. 5C shows bar charts indicating activation of DRD2-4 and HRH2-4 by select species and strains as measured by PRESTO-Tango (n=3 technical replicates per isolate).

FIG. 5D is a bar chart showing quantification of dopamine, phenethylamine and tyramine production by M. morganii. Supernatants from 24-hour cultures of M. morganii in gut microbiota medium were analyzed by Electron Spray Ionization-Triple Quadrupole-Mass Spectrometry (ESI-QQQ-MS) and compared to those of media controls.

FIG. 5E is a plot showing quantification of histamine production by 144 isolates of human gut bacteria. All bacteria were grown in gut microbiota media for 48 hours and then supernatants were probed for histamine production via ELISA.

FIG. 5F shows mass spectrometric traces of metabolite production by M. morganii, which can directly convert L-Phe and L-His into phenethylamine and histamine, respectively. However, no conversion of L-Tyr to tyramine or L-DOPA to dopamine was observed. M. morganii was cultured in minimal medium (MM) with or without additional L-Phe, L-His, L-Tyr or L-DOPA for 48 hours. Metabolite production was analyzed by Liquid Chromatography-Mass Spectrometry (LC-MS).

FIG. 5G is a heatmap showing M. morganii-derived phenethylamine and histamine activate DRD2-4 and HRH2-4, respectively. M. morganii were cultured as described in F and supernatants were screened for activity against DRDs and HRHs by PRESTO-Tango.

FIGS. 6A and 6B show bar charts indicating that diverse human gut bacteria activate DRDs and HRHs. The charts show activation of DRD1-5 (FIG. 6A) and HRH1-4 (FIG. 6B) by supernatants from 144 human gut bacteria cultured in gut microbiota media (MM) as measured by PRESTO-Tango.

FIG. 7A is a chart showing the activation of CHRMs and DRDs by titrating doses of acetylcholine and dopamine as measured by PRESTO-Tango.

FIG. 7B is a heat map showing activation of GPCRs by defined GPCR ligands as measured by PRESTO-Tango. Activation is depicted on a log 2 scale as a heatmap of 314 GPCRs versus ligands.

FIGS. 8A-8F show identification of M. morganii-derived compounds that activate DRDs and HRHs. Data are representative of at least two independent experiments. FIG. 8A is an illustration of mammalian dopamine metabolism. FIG. 8B depicts data showing that phenethylamine and tyramine serve as selective DRD2/DRD3/DRD4 agonists. Activation of DRD1-5 by metabolites in the mammalian dopamine metabolism pathway was measured via PRESTO-Tango. FIG. 8C is a calibration curve for phenethylamine and tyramine on ESI-QQQ-MS instrument. FIG. 8D is a chart showing quantification of phenethylamine production by M. morganii strains via ESI-QQQ-MS/MS. FIG. 8E shows DRD1-5 activation by titrating doses of tyramine, dopamine and phenethylamine was measured by PRESTO-Tango (8D). FIG. 8F shows OD values for 24-hour cultures of M. morganii grown in minimal medium (MM) with or without L-Phe, L-Tyr, L-DOPA or L-His.

FIGS. 9A-9H show commensal-derived histamine promotes colon motility and M. morganii-derived phenethylamine combined with MAOI triggers lethal phenethylamine poisoning. Data in all panels are representative of at least two independent experiments.

FIG. 9A shows production of histamine by M. morganii and L. reuteri is enhanced by additional L-His. Four strains of L. reuteri and one strain of M. morganii were cultured in Gifu medium with or without supplemental L-His (n=3 per group) and histamine concentrations in the supernatants were measured by ELISA after 48 hours. The addition of L-His increased histamine production by L. reuteri C93, C94 and M. morganii C135 (all histamine producers), but not L. reuteri C88 and C89. FIG. 9B depicts an experimental design to test in vivo histamine production and the effects of histamine-producing bacteria on colon motility.

FIG. 9C shows bar charts of results in which groups of germ-free C57Bl/6 mice were colonized with mock communities of 9 or 10 phylogenetically diverse human gut bacteria (Mock Community A or B) or monocolonized with M. morganii, L. reuteri C88 or C93. Mice were fed a conventional diet with or without administration of 1% L-His ad libitum. Histamine concentrations in cecal and colonic extracts and feces were measured via ELISA. FIG. 9D is a bar chart showing M. morganii- or L. reuteri C93-derived histamine enhances colon motility. Fecal output for mice treated as described in B were measured by counting the number of fecal pellets produced by a single mouse in one hour.

FIG. 9E is a trace showing that M. morganii produces phenethylamine in vivo. Germ-free mice were colonized with M. morganii and treated with or without the MAOI phenelzine. Phenethylamine concentration in colonic extracts was examined using ESI-QQQ-MS/MS. In the graph of FIG. 9F, mice colonized with M. morganii exhibit lethal phenethylamine poisoning after treatment with the MAOI phenelzine. Germ-free C57Bl/6 mice were monocolonized with M. morganii for one week before treatment with phenelzine in the drinking water. Survival is depicted on a Kaplan-Meier curve. n=4 mice per group. In FIGS. 9G and 9H, M. morganii-colonized mice treated with phenelzine accumulated phenethylamine in the cecum, colon, serum and brain. M. morganii and B. theta monocolonized C57Bl/6 mice were treated with or without the MAOI phenylzine in the drinking water. Phenethylamine was detected in the cecum, colon, serum or brain via ESI-QQQ-MS (9G) or DRD2 PRESTO-Tango (9H).

FIGS. 10A-10F show M. morganii localization and production and accumulation of systemic phenethylamine in vivo, as related to FIGS. 9A-9H. Data are representative of at least two independent experiments. In FIG. 10A, groups of germ-free C57Bl/6 mice were colonized with mock communities of 9 or 10 phylogenetically diverse human gut bacteria (Mock Community A or B) or monocolonized with M. morganii, L. reuteri C88 or C93. Mice were fed a conventional diet with or without administration of 1% L-His ad libitum. Histamine concentrations in serum were measured via ELISA. FIGS. 10B-10D show M. morganii primarily inhabits the cecum and colon. Germ-free mice were colonized with mock communities of 9 or 10 phylogenetically diverse gut microbes (Mock community A and B, respectively) with or without M. morganii. CFUs from M. morganii can be distinguished from other bacteria based on their purple halos when plated on modified Niven's agar. Gastric, small intestinal, cecal and colonic contents from mice colonized with Mock communities A or B, and M. morganii, were plated on Modified Niven's agar to determine M. morganii colonization levels at various intestinal loci. FIG. 10E shows quantification of phenethylamine (PEA) in cecum, colon, and serum from mice monocolonized with M. morganii and treated with or without phenelzine (MAOI) via ESI-QQQ-MS/MS. FIG. 10F shows accumulation of phenethylamine (PEA) in sera and brains of mice monocolonized with M. morganii and treated with or without phenelzine (MAOI) as measured via ESI-QQQ-MS/MS.

FIGS. 11A-11G show that a unique strain of B. thetaiotaomicron C34 is a prolific producer of L-Phe and activates GPR56/AGRG1. Data in all Figures except for 11A, 11B, and 11E are representative of at least three independent experiments.

FIGS. 11A and 11B are diagrams showing that activation of orphan GPCRs by supernatants from 144 diverse human gut bacteria grown in gut microbiota medium (FIG. 11A) or Gifu (FIG. 11B) as measured by PRESTO-Tango. Screening results are displayed on a phylogenetic tree of orphan GPCRs that was constructed and visualized with equal branch lengths using gperdb.org, PHYLIP and jsPhyloSVG. The class A orphans are GPR21, GPR52, GP143, GPR32, GPR1, GPR152, MAS, MRGRF, MRGX2, MRGX4, MRGX1, MRGX3, MRGRD, MAS1L, MRGRE, MRGRG, GP182, GPR15, GPR25, GPR82, GPR34, GPR87, GP171, GP183, GP132, PSYR, GPR4, OGR1, GPR17, GPR174, GPR35, GPR20, GPR31, GPR88, MTR1L, GPR84, GP148, GP142, GPR19, GPR82, GPR27, GP172, GPR85, GP150, GPR6, GP146, GPR26, GPR78, GP135, GP161, GP101, GPR12, GPR3, GPR6, TAAP2, TAAP5, TAAP9, TAAP6, TAAP8, GP151, ETBR2, GPR37, GPR39, GPR75, GPR45, GPR63, GPR22, GP141, GP160, GP153, GP162, and GP149. The class C orphans are GP156, GP158, GPC6A, GPC5B, GPC5C, GPC5D, and RAI3. The ADGRA group is AGRA1, AGRA2, and AGRA3. The AGRGD group is AGRD1 and AGRD2. The ADGRG group is AGRG1, AGRG2, AGRG3, AGRG5 and AGRG6. The ADGRF group is AGRF1, AGRF2, AGRF3, AGRF4 and AGRF5. The other GPCR orphan is GP157. Shading intensities represent the magnitude of activation over media and radii of circles represent the number of bacteria that activated a given GPCR by more than two-fold. FIG. 11C depicts bar charts showing a single isolate C34 assigned to the species Bacteroides thetaiotaomicron activates GPR56/AGRG1 when cultured in gut microbiota medium (GMM) or Gifu medium. Activation of GPR56/AGRG1 by supernatants from 144 human gut isolates was measured via GPR56 PRESTO-Tango.

FIG. 11D is a bar chart showing that B. theta strain C34 uniquely activates GPR56/AGRG1. Activation of GPR56/AGRG1 by supernatants from diverse species and strains from the genera Bacteroides and Parabacteroides culture in GMM was measured via GPR56 PRESTO-Tango. FIG. 11E is a bar chart showing that B. theta C34-produced L-Phe activates GPR56/AGRG1. B. theta C34 supernatants were fractionated via reversed-phase HPLC and fractions were evaluated for activation of GPR56/AGRG1 via GPR56 PRESTO-Tango. The active fraction (F11) contained a primary constituent that was identified via LC-MS, HRMS-ESI-QTOF, NMR, and advanced Marfey's analyses as L-Phe. FIG. 11F is a graph showing that L-Phe preferentially activates the orphan receptor GPR56/AGRG1. Activation of GPR56/AGRG1 by titrating doses of pure L-Phe, L-Tyr, L-Trp, and L-His was measured via GPR56 PRESTO-Tango. FIG. 11G is a graph showing that the extracellular domain of GPR56/AGRG1 is indispensable for GPR56/AGRG1 activation by L-Phe. Activation of GPR56 or GPR56-ANT (a mutant lacking the extracellular domain) by titrating doses of L-Phe was measured via PRESTO-Tango.

FIGS. 12A-12D show the effect of different bacterial and culture media on bacterial growth and GPR56/AGRG1 activation, and structural characterization of B. theta C34 agonist L-Phe, as related to FIGS. 11A-11G. FIG. 12A shows OD₆₀₀ values of indicated Bacteroides and Parabacteroides strains cultured in gut microbiota medium (GMM) for 24 hours. FIG. 12B shows a ¹H NMR spectrum of active fraction 11 in MeOD revealed Phe as the major component. FIG. 12C shows the results of an advanced Marfey's analysis that verified the stereochemistry of Phe in fraction 11 to be L-Phe. D-Phe in the active fraction was not detected. FDAA is 1-fluoro-2,4-dinitrophenyl-5-L-alanine amide (Marfey's Reagent). FIG. 12D shows that L-Phe stereoselectively activates the orphan receptor GPR56/AGRG1. Activation of GPR56/AGRG1 by titrating doses of pure L-Phe, L-Tyr, L-Trp, L-His, D-Phe, D-Tyr, D-Trp, and D-His was measured via GPR56 PRESTO-Tango.

FIGS. 13A-13D illustrate data showing that L-Phe activates GPR97/AGRG3, a close relative of GPR56/AGRG1. Data are representative of at least three independent experiments. FIG. 13A is a heatmap showing that L-Phe activates GPR56/AGRG1 and GPR97/AGRG3. Activation of all orphan, adhesion and other potential amino acid-sensing GPCRs by L-Phe was evaluated via PRESTO-Tango. FIG. 13B is a graph showing that L-Phe specifically activates GPR97/AGRG3. Activation of GPR97/AGRG3 by titrating doses of L-Phe, L-Tyr, L-Trp, and L-His was measured via GPR97 PRESTO-Tango. FIG. 13C is a graph showing that the extracellular domain of GPR97/AGRG3 is indispensable for GPR97/AGRG3 activation by L-Phe. Activation of GPR97 or GPR97-ANT (a mutant lacking the extracellular domain) by titrating doses of L-Phe was measured via PRESTO-Tango. FIG. 13D is a phylogenetic tree showing that GPR56/AGRG1 and GPR97/AGRG3 are evolutionarily related. The phylogenetic tree for a subset of GPCRs, including all adhesion GPCRs, was constructed and visualized with equal branch lengths using gperdb.org, PHYLIP and jsPhyloSVG.

FIGS. 14A-14E depict data showing that active metabolic exchange between two commensals supports production of phenethylamine. The data are representative of at least two independent experiments. FIGS. 14A and 14B are data showing that B. theta C34 can directly synthesize L-Phe. L-Phe concentrations in supernatants from C34 grown in a minimal medium (SACC) lacking L-Phe were evaluated by LC-MS (14A) and quantitated by ESI-QQQ-MS/MS (14B). FIG. 14C is a graph showing that B. theta C34 produces L-Phe in vivo. Groups of germ-free C57Bl/6 mice fed a conventional diet or a defined diet lacking L-Phe were colonized with or without B. theta C34. Fecal L-Phe concentrations were measured by ESI-QQQ-MS/MS one week after colonization. FIG. 14D is a trace showing that M. morganii consumes B. theta C34-derived L-Phe to produce phenethylamine in vitro. B. theta C34 cultures were grown in SACC medium lacking L-Phe. Supernatants of C34 cultures were later incubated with M. morganii. ESI-QQQ-MS/MS traces of L-Phe and phenethylamine (PEA) levels in these cultures are depicted here. FIG. 14E is a graph showing that B. theta C34 and M. morganii can participate in active metabolic exchange to produce phenethylamine in vivo. Groups of germ-free C57Bl/6 mice were monocolonized with M. morganii or co-colonized with B. theta C34 and M. morganii, fed a diet lacking L-Phe and treated with the MAOI phenelzine. Activation of DRD2 by phenethylamine in cecal and colonic extracts was measured by PRESTO-Tango.

FIG. 15A is a graph showing that Crohn's disease patients display an increased presence and relative abundance of histidine decarboxylase genes in their gut microbiome. Metagenomic data from fecal samples collected from healthy controls (nonIBD), Crohn's disease (CD) and ulcerative colitis (UC) were downloaded from the NIH-funded Human Microbiome Project and analyzed for the presence and relative abundance of histidine decarboxylase genes. The number of total samples that contained detectable histidine decarboxylase genes and the number of patients that had detectable genes in any given sample are shown (all participants donated multiple samples at distinct timepoints).

FIG. 15B is a graph showing that Crohn's disease patients display an increased presence and relative abundance of histidine decarboxylase genes encoded by M. morganii (Morganella) in their gut microbiome. Metagenomic data from fecal samples collected from healthy controls (nonIBD), Crohn's disease (CD) and ulcerative colitis (UC) were downloaded from the NIH-funded Human Microbiome Project and analyzed for the presence and relative abundance of the M. morganii histidine decarboxylase gene. The number of total samples that contained detectable histidine decarboxylase genes and the number of patients that had detectable genes in any given sample are shown (all participants donated multiple samples at distinct timepoints).

FIG. 16 is a graph showing the results of an experiment in which a new ultra-highthroughput combinatorial GPCR screening technology PRESTO-Salsa enables simultaneous screening of hundreds of GPCRs in a single well of a 96 well plate. The data was generated according to the protocol described in Example 9.

DETAILED DESCRIPTION OF THE INVENTION

In one aspect is provided a method allowing for parallel screening of multiple GPCRs (e.g., all 300 or more conventional GPCRs) in a single tube. Exemplary embodiments of this method are referred to herein as PRESTO-Salsa, in which instead of a luciferase reporter, reporter plasmids that encode unique nucleic acid barcodes are used.

Without wishing to be bound by theory, trillions of bacteria that constitutively colonize human intestines (gut microbiota) produce tens of thousands of unique small molecules that can potentially affect nearly all aspects of human physiology, from regulating immunity in the gut to shaping mood and behavior. However, this complexity can make it challenging to identify biologically relevant microbiota metabolites hidden in a sea of other chemicals. In other words, given the presence of tens of thousands of unique, unannotated metabolites in the microbiota metabolome, it can be difficult to decide which features should be prioritized for in-depth examination and characterization.

The inventors propose a revolutionary new approach to these problems. Without wishing to be bound by theory, the inventors describe host sensing of microbiota metabolites as a lens to illuminate the ‘dark matter’ that constitutes the majority of the bioactive microbiota metabolome. The inventors hypothesize that the hundreds of G-protein coupled receptors (GPCRs) encoded in the human genome can be used to identify novel bioactive microbiota metabolites from complex mixtures.

A new approach is described herein to investigate the bioactive microbiota metabolome where we used the sensing of microbiota metabolites by host GPCRs as a lens to illuminate bioactive metabolites produced by individual gut microbes. The examples described herein describe how the approach revealed a plethora of novel microbiota metabolite-GPCR interactions of potential physiological importance. For example, the inventors uncovered a diet-microbe-host axis that influences intestinal motility through the microbial production of histamine and a microbe-microbe-host axis that results in the production of the potent trace amine phenethylamine. Both of these axes can have profound effects on local and systemic host physiology. The functional profiling-based approach to understanding the contribution of the microbiota to human physiology described herein may be broadly applicable to understanding and illuminating diverse features of the bioactive microbiota metabolome.

An existing low-throughput GPCR-screening technology (PRESTO-Tango) was used by the inventors to identify metabolite mixtures from individual human gut bacteria that activated GPCRs involved in carcinogenesis, mood regulation, and immunity, as well as ‘orphan’ GPCRs whose natural ligands have evaded discovery for decades. However, because of inherent technical limitations, PRESTO-Tango can only be applied to a relatively small number of metabolite mixtures that are readily available in large quantities—e.g., metabolites produced by bacterial strains that can be cultured in vitro. PRESTO-Tango can thus only capture a small proportion of the overall bioactive microbiota metabolome, which normally results from complex in vivo interactions between multiple microbes, dietary compounds, and the host itself.

The inventors have leveraged recent developments in next-generation sequencing and nucleic acid barcode analyses to develop PRESTO-Salsa.

In one aspect a method is provided for high-throughput screening of microbiota metabolites capable of activating a plurality of G-protein coupled receptors (GPCRs). A plurality of non-adherent mammalian cells is provided. Each cell comprises (i) a first nucleic acid molecule encoding a first fusion protein comprising a GPCR linked to the transcription factor tTA via a cleavage site for Tobacco Etch Virus nuclear inclusion A protease (TEV protease), (ii) a second nucleic acid molecule encoding a second fusion protein comprising β-arrestin and TEV protease configured to cleave the TEV protease site on the first fusion protein, and (iii) a third nucleic acid molecule comprising a barcode sequence operably linked to a promoter specifically activated by the tTA transcription factor, wherein each barcode sequence is specific for an individual GPCR. The plurality of cells is contacted with a plurality of microbiota metabolites. The barcodes are sequenced. A determination is made as to which barcode sequences are increased in the presence of the metabolites as compared to a control where no metabolites are present.

Transcription of a barcode can indicate the activation of a GPCR. Without wishing to be bound by theory, when a GPCR is activated, the conformation of the GPCR changes such that a fusion protein comprising β-arrestin and TEV protease is recruited. The TEV protease then cleaves the protease site linking the tTA transcription factor and the GPCR so as to release the tTA transcription factor, which in turn drives transcription of a barcode operatively linked to the promoter.

A variety of GPCRs can be used. The GPCR may be a non-olfactory GPCR, an orphan GPCR, or a human GPCR. In preferred embodiments, the GPCR is one of the 314 conventional GPCRs described herein.

The microbiota metabolites may come from any number of samples, such as a fecal sample, or a sample from the gastrointestinal tract. The fecal sample or the sample from the gastrointestinal tract may be cultured and/or exposed to nutrients so as to enhance production of a particular microbiota metabolite. For example, additional L-Phe and L-His can be supplied to increase sensitivity of detection of M. morganii, which can directly convert L-Phe and L-His into phenethylamine and histamine, respectively. The sample may also be fractionated based on chemical properties, for example by reversed phase HPLC.

Without wishing to be bound by theory, the use of barcodes can greatly improve sensitivity of detection and allow for high-throughput screening. A larger number of cells expressing a wider variety of unique GPCR-barcode pairs can be used. Highly sensitive quantitative methods can be used to assay for the expression of all barcodes, e.g., as described in Example 1.

As shown in FIG. 1A, the traditional PRESTO-Tango approach connects GPCR activation to luciferase expression by linking Beta-arrestin recruitment following ligand binding to the release of a transcription factor (tTA) that is tethered to the GPCR of interest. The inventors modified this system by replacing the luciferase reporter with reporter plasmids that encode unique nucleic acid barcodes, as shown in FIG. 1B. The major novelty of PRESTO-Salsa is the use of transcription of unique barcodes to enable multiplexing of hundreds of GPCRs in a single well. This improves upon the PRESTO-Tango technology by increasing potential throughput by multiple orders of magnitude and allowing for examination of previous low-volume samples. PRESTO-Salsa was tested as shown in Example 1 and in FIGS. 3 and 4 .

By pooling cells that express unique GPCR-barcode pairs and then quantifying the expression of specific barcodes after stimulation, activation or inhibition of hundreds of GPCRs in a single tube can be simultaneously screened for. See, FIG. 1C.

The method can further comprise identifying a bacterial strain producing the specific metabolite which activates the specific GPCR. Bacterial strains can be classified by 16S rRNA sequencing, for example, or by whole genome sequencing.

Individual bacterial strains can be isolated and cultured from a particular sample of interest, with metabolites from each cultured strain further tested in the various PRESTO-Salsa embodiments described herein. Alternatively, a bacterial strain can be added to a microbiota sample to assess the metabolites produced by the bacterial strain in the context of a given microbiome. The microbiota sample can then be cultured to allow the added bacterial strain to produce additional metabolites. A differential analysis of the barcodes produced by the PRESTO-Salsa method can be undertaken.

It was found that dozens of human gut bacteria from diverse phyla, families, species, and strains produced small molecules that activated various GPCRs, including both well-characterized GPCRs and orphan GPCRs. Patterns of metabolite production were observed that were largely predictable based on phylogeny, as well as strain-specific differences within a given species. Future studies will be necessary to determine when and why specific pathways are conserved or not in distinct species and strains. Metabolites resulting from core metabolic processes essential to a given microbe might be highly conserved, while metabolites involved in competitive processes may show considerable strain variation that could depend on the niche that is occupied and the need to compete with other bacterial species, non-bacterial microbes, or with the host. Regardless of the initial impetus for microbial metabolite expression, the data described herein support the concept that human-associated microbes exhibit impressive regulatory capabilities and are likely one of the richest sources of small molecules that impact human biology.

The high throughput of PRESTO-Salsa can allow for ability to easily examine biological or technical replicates, as well as perform dose response curves, for all samples. Finally, PRESTO-Salsa may be resistant to well-known off-target effects of certain chemicals on luciferase stability and thus may also reduce false positives.

PRESTO-Salsa provides a major technical advantage in that hundreds of receptors may be screened simultaneously in a single tube using small sample input volumes. PRESTO-Salsa is modular and flexible, and thus can be expanded to include other receptor families. Also, PRESTO-Salsa can easily be modified to simultaneously monitor multiple signaling outputs downstream of the same receptors (e.g., G-protein based signaling versus B-arrestin based signaling by GPCRs) to allow for identification of biased agonists and antagonists. PRESTO-Salsa enables screening of precious low-volume samples against hundreds of sensors while a similar sample volume used in PRESTO-Tango would only have been sufficient to examine the effects on one or two receptors. PRESTO-Salsa can provide for imminent scalability and automation. A major economic advantage of PRESTO-Salsa is that PRESTO-Salsa can enable simultaneous screening of hundreds of receptors in a single tube using low input volumes; prior technologies would require multiple plates of cells and a large input sample volume to accomplish this same goal. The use of next-gen sequencing as the final readout in PRESTO-Salsa is considerably cheaper than the prior Luciferase based screening (especially as next-gen sequencing continues to decline in cost). The cost of screening using PRESTO-Salsa may be lower than that of established assays by at least an order of magnitude.

The microbiota metabolome results from a complex web of interactions between multiple microbial species and strains, environmental inputs (e.g., diet), and host factors. Using a reductionist approach, the inventors discovered two bacterial isolates that traffic in the same small molecule: a unique strain of B. theta that is a prolific producer of L-Phe and M. morganii, which efficiently converts L-Phe into phenethylamine. The approaches described herein can reveal metabolic exchanges that may be missed when examining endpoint microbiota metabolomes produced by complex mixtures of microorganisms (e.g., complete gut microbial communities).

In various embodiments of the methods described herein, any GPCR agonists can be further tested in vivo, for example to examine the possibility that production of GPCR agonists by specific microbes would shape host physiology in vivo. The data in the examples show that histamine production by M. morganii or L. reuteri promotes increased colon motility and that feeding with exogenous histidine further increases microbial production of histamine and colonic motility. M. morganii monocolonized mice fed with histidine exhibited elevated levels of systemic histamine, indicating that microbiota-derived histamine may also shape systemic immune responses.

The inventors' studies also uncovered a specific Bacteroides strain that uniquely produces high levels of the essential amino acid L-Phe and revealed that L-Phe could activate the orphan GPCRs GPR56 and GPR97. GPR56 is highly expressed in the small intestine and human pancreatic islets (Amisten et al., 2013; Duner et al., 2016), and L-Phe concentrations in the jejunum can reach concentrations up to 2 mM after a meal (Adibi, 1973). Thus, GPR56 may act as a nutrient sensor to regulate digestion and satiety.

Although L-Phe levels in the serum usually are well below the levels necessary to activate GPR56/97, patients with phenylketonuria (PKU) who cannot degrade L-Phe exhibit serum concentrations of L-Phe higher than 1 mM (Williams, 2008). Thus, activation of GPR56 and/or GPR97 may contribute to some of the symptoms of PKU. Notably, GPR56 is highly expressed in neural progenitor cells, oligodendrocytes, astrocytes and microglia (Giera et al., 2018; Haitina et al., 2008), and GPR56/AGRG1 deficiency can cause severe neurodevelopmental diseases such as bilateral frontoparietal polymicrogyria (BFPP) (Sotnikova et al., 2004).

Without wishing to be bound by theory, dietary amino-acid availability can be important in the production of biogenic amines that can shape host physiology. The studies herein can highlight other members of the microbiota as an alternative source of substrates that are often thought of as primarily derived from diet (e.g., essential amino acids). Microbial-produced amino acids may potentially supplement or even replace dietary amino acids in microbial biotransformations. Microbe-derived L-Phe may be used as a substrate for biotransformation by M. morganii using a simplified and highly-artificial diet that lacks L-Phe. However, bacterial L-Phe may also be important under physiological conditions. For example, dietary amino acids are largely absorbed in the small intestine (Adibi, 1973); thus, colonic microbes such as M. morganii have relatively limited access to dietary amino acids as compared to small intestinal organisms. Also, low-protein diets naturally decrease microbial access to dietary amino acids, and fasting may reduce intestinal amino acid availability even further (Pezeshki et al., 2016). Thus, microbial production of amino acids in the colon may play a critical role in the production of various bioactive microbiota metabolites under a variety of physiologically relevant conditions.

Also provided are various therapeutic and diagnostic methods.

In one aspect is provided a method of preventing or treating monoamine oxidase inhibitor (MAOI)-induced toxicity in a subject. An antibiotic effective to target a bacterial strain comprising a nucleic acid sequence encoding a phenethylamine production gene is administered.

In a related aspect is provided a method of preventing or treating monoamine oxidase inhibitor (MAOI)-induced toxicity in a subject. A small molecule antagonist of a bacterial phenethylamine synthesis enzyme is administered.

In various embodiments, the method of preventing or treating MAOI-induced toxicity in a subject comprising administering an antibiotic effective to target Morganella spp.

Although M. morganii was previously reported to produce dopamine, the inventors found that all isolates of M. morganii primarily produced the potent trace amine phenethylamine rather than dopamine or tyramine. The inventors also found that treatment of M. morganii monocolonized mice with an MAOI led to systemic accumulation of phenethylamine and mortality. Phenethylamine is a potent neuroactive chemical that, unlike dopamine and tyramine, can readily cross the blood-brain barrier (Oldendorf, 1971). The effects of phenethylamine are thought to be mediated primarily through activation of the trace amine-associated receptors and subsequent release of norepinephrine and dopamine (Borowsky et al., 2001; Bunzow, 2001; Sotnikova et al., 2004). However, the data here suggests that phenethylamine also can act as a selective agonist for DRD2-4. It will be fascinating to dissect the cellular and molecular mechanisms by which microbiota-derived phenethylamine can influence host biology both locally and systemically.

Without wishing to be bound by theory, the inventors' findings suggest that interindividual variability in microbial production of phenethylamine may explain some of the variable effects of MAOIs on depression; this possibility is particularly intriguing given the reported beneficial effects of phenethylamine on mood and the ability of phenethylamine to cross the blood-brain barrier (Irsfeld et al., 2013). In addition, biogenic amine production by gut microbes may explain some of the side effects of MAOIs.

One of the most prominent adverse events associated with MAOIs is ‘tyramine poisoning’, which typically results from ingestion of food products containing high levels of tyramine, such as certain cheeses (Fiedorowicz, 2004). The data herein indicates that specific gut microbes also act as a source of biogenic amines that may have similar effects on host physiology and that it is possible that pharmacological inhibitors of biogenic amine receptors that are meant to act at specific sites (e.g., in the brain) may also impinge upon natural host-microbiota interactions.

Also provided is a method of treating a disease or condition caused by decreased MAO activity in a subject. An antibiotic effective to target a bacterial strain comprising a nucleic acid sequence encoding a phenethylamine production gene is administered.

In a related aspect is provided a method of treating a disease or condition caused by decreased MAO activity in a subject. A small molecule antagonist of a bacterial phenethylamine synthesis enzyme is administered.

The bacterial strain may produce phenethylamine, such as M. morganii. The bacterial strain may secrete phenethylamine. Various diseases or conditions caused by decreased MAO activity can be treated, including Brunner syndrome, autism or anti-social behavior. The subject may express a MAOA-L variant.

Various antibiotics effective to target Morganella morganii may be used, such as cefepime, piperacillin, tazobactam, ceftazidime, cefotaxime, ceftibuten, meropenem, doripenem, ertapenem, a fluoroquinolone, or an aminoglycoside.

Also provided is a method of treating depression in a subject comprising administering a bacterial strain comprising a nucleic acid sequence encoding a phenethylamine production gene. An anti-depressant, such as an MAOI, can also be administered to the subject.

Also provided is a method for evaluating potential toxicity of a monoamine oxidase inhibitor (MAOI) in a subject. A gastrointestinal microbiota sample is obtained from the subject. The sample is assayed for the presence of a bacterial strain comprising a phenethylamine production gene, such as Morganella morganii. The levels of the bacterial strain can also be quantitatively assayed so as to further assess the risk of potential MAOI toxicity. The presence of, and/or levels of, the bacterial strain can be used to assess the risk of MAOI toxicity. From this information, a different anti-depressant or therapeutic besides a MAOI may be administered. Alternatively, the dosage levels of MAOI may be adjusted or reduced.

Also provided is a method for evaluating method for evaluating potential efficacy of an MAOI in a subject. The method comprises taking a fecal sample or a sample from the gastrointestinal tract of the subject, and assaying for the presence of a bacterial strain (e.g., Morganella morganii) comprising a nucleic acid sequence encoding a phenethylamine production gene. A quantitative assay for the amount of the bacterial strain may also be conducted. The subject may then be treated with an MAOI. The dosage or type of MAOI can be adjusted based on the presence of, and/or amount of, the bacterial strain.

Also provided is a method for method of preventing or treating histamine-induced gastrointestinal disease in a subject. Notably, the inventors discovered that histidine decarboxylases, including M. morganii histidine decarboxylase, are enriched in patients with Crohn's disease as compared to healthy controls or UC patients (see FIGS. 15A and 15B), which may implicate histamine production by bacteria in CD. One or more antibiotics effective to target a bacterial strain comprising a nucleic acid sequence encoding a histamine production gene is administered to the subject. In a related aspect is provided preventing or treating histamine-induced gastrointestinal disease in a subject where a small molecule antagonist of histamine synthesis enzyme is administered. Exemplary small molecule antagonists of histamine synthesis enzymes include, but are not limited to, histidine decarboxylase inhibitors, such as rugosin D, rugosin A methyl ester, tellimagrandin II, rugosin A, pinocembrin, α-fluoromethylhistidine, brocresine, lecanoric acid, 2-hydroxy-5-carbomethoxybenzyloxyamine, and aminooxy analogs of histamine. Morganella histidine decarboxylase may be inhibited by rugosin D, rugosin A methyl ester, tellimagrandin II, and rugosin A. In a related aspect, general or specific histamine receptor antagonists are administered.

An exemplary disease is histamine intolerance, which can mimic the symptoms of food allergy. Another is diarrhea. Histamine-induced gastrointestinal diseases can be caused by, or exacerbated by, interference with the activity of the enzymes DAO and HNMT. Without wishing to be bound by theory, the presence of, or an excess amount of, L. reuteri or a bacterium of Morganella spp. can cause or exacerbate intestinal disease. These can directly convert L-His into histamine.

Another exemplary disease is inflammatory bowel disease. The abundance of Morganella spp. encoding histidine decarboxylase is increased in Crohn's disease versus healthy controls and ulcerative colitis (FIG. 15B). Without wishing to be bound by theory, the presence of, or an excess amount of Morganella spp. or histidine decarboxylase may cause or exacerbate Crohn's disease. Also provided is a method to treat patients that harbor Morganella spp. or other bacteria that encode histidine decarboxylase or an excess amount of bacteria that encode histidine decarboxylase. One or more antibiotics effective to target a bacterial strain comprising a nucleic acid sequence encoding a histamine production gene is administered. In a related aspect is provided preventing or treating histamine-induced gastrointestinal disease in a subject where a small molecule antagonist of histamine synthesis enzyme is administered. In a related aspect, general or specific histamine receptor antagonists are administered.

Also provided is a method of preventing or treating an allergy, or asthma, in a subject. One or more antibiotics effective to target a bacterial strain comprising a nucleic acid sequence encoding a histamine production gene is administered. The bacterial strain can be L. reuteri or a bacterium of Morganella spp. Without wishing to be bound by theory, the presence of either or both strains can increase overall histamine levels in the subject, which would induce or exacerbate allergies or asthma. Exemplary antibiotics include cefepime, piperacillin, tazobactam, ceftazidime, cefotaxime, ceftibuten, meropenem, doripenem, ertapenem, a fluoroquinolone, or an aminoglycoside.

The antibiotic may be selected for based on the outcome of a method for evaluating potential effectiveness of an antibiotic to treat a gastrointestinal condition or disease, allergy or asthma in a subject. The method comprises a) taking a fecal sample or a sample from the gastrointestinal tract of the subject, and b) assaying for the presence of a bacterial strain (e.g., bacterium of Morganella spp.) comprising a nucleic acid sequence encoding a histamine production gene. The method can comprise assaying for the amount of the bacterial strain present in the gastrointestinal tract. The subject may be treated with the antibiotic. In some embodiments, an adjusting or determining the dosage of the antibiotic is undertaken based on the presence and/or amount of the bacterial strain present.

Also provided is a method of preventing or treating a disease or condition resulting from production of phenethylamine, the method comprising administering one or more antibiotics effective to target a bacterial strain producing L-phenylalanine. In a related aspect is provided a method of preventing or treating a disease or condition resulting from production of phenethylamine, the method comprising administering a small molecule antagonist of a phenethylamine synthesis enzyme. The small molecule antagonist may be a Shikimate pathway inhibitor. The small molecule antagonist may be an antagonist of aromatic L-amino acid decarboxylase (AADC or AAAD), such as carbidopa, benserazide, methyldopa, 3′,4′,5,7-Tetrahydroxy-8-methoxyisoflavone (DFMD), 3-hydroxybenzylhydrazine, 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2), and α-difluoromethyl DOPA.

Further provided is a method of preventing or treating phenylketonuria (PKU) in a subject, the method comprising administering one or more antibiotics effective to target a bacterial strain producing L-phenylalanine. Also provided is a related method of preventing or treating phenylketonuria (PKU) in a subject, the method comprising administering a small molecule targeting the bacterial phenylalanine synthesis enzymes of bacterial strains secreting L-phenylalanine for the treatment or prevention of diseases resulting from overproduction of L-phenylalanine.

Exemplary bacterial strains include B. thetaiotaomicron (e.g., strain C34). The method may comprise administration of an antibiotic effective to target the bacterial strain, such as ampicillin, clavulanate, tazobactam, a cephamycin, ticarcillin, piperacillin, a cephalosporin, a carbapenem, clindamycin, lincomycin, chloramphenicol, a nitroimidazole, a fluoroquinolone. Alternatively, a combination of antibiotics may be administered, such as (a) a combination of ampicillin and sulbactam, (b) a combination of ticarcillin and clavulanate, or (c) a combination of piperacillin and tazobactam.

In various embodiments of the above methods involving administration of an antibiotic, a probiotic composition may be administered. The probiotic composition may be administered after a course of antibiotics is administered, for example. Without wishing to be bound by theory, the probiotic composition may be effective to colonize the gut with different bacteria and prevent the targeted bacteria from quickly reestablishing itself. In additional embodiments, the antibiotic can be administered in a cyclic manner with the probiotic composition. The antibiotic could even be administered concurrently with the probiotic composition.

Also provided is a kit for evaluating potential toxicity of an MAOI in a subject. The kit can comprise a nucleic acid, antibody, or other reagent capable of binding specifically to a nucleotide or protein expressed by a bacterial strain, where the bacterial strain comprises a nucleic acid sequence encoding a phenethylamine production gene.

Also provided is a kit for evaluating potential effectiveness of an antibiotic to treat a gastrointestinal condition or disease, allergy or asthma in a subject. The kit comprises a nucleic acid, antibody, or other reagent capable of binding specifically to a nucleotide or protein expressed by a bacterial strain, where the bacterial strain comprises a nucleic acid sequence encoding a histamine production gene (such as one detected in Crohn's disease patients according to data shown in FIGS. 15A and 15B). The kit may be used to select for a patient who would benefit from treatment with a small molecule inhibitor of an enzymatic product of a histamine production gene (e.g., histamine decarboxylase inhibitor).

EXAMPLES

The present invention is also described and demonstrated by way of the following examples. However, the use of these and other examples anywhere in the specification is illustrative only and in no way limits the scope and meaning of the invention or of any exemplified term. Likewise, the invention is not limited to any particular preferred embodiments described herein. Indeed, many modifications and variations of the invention may be apparent to those skilled in the art upon reading this specification, and such variations can be made without departing from the invention in spirit or in scope. The invention is therefore to be limited only by the terms of the appended claims along with the full scope of equivalents to which those claims are entitled.

The following table shows the reagents used throughout the following examples:

REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial Strains B. fragilis ATCC 25285 B. ovatus ATCC 8483 B. thetaiotaomicron ATCC 29741 B. uniformis ATCC 8492 M. morganii ATCC 25830&49948 Chemicals, peptides, and Recombinant Proteins Gifu Anaerobic Broth VWR 11007-214 Bright-Glo ™Luciferase Promega E2620 Assay System Histamine Elisa Kit Enzo Life Sciences ENZ-KIT140-0001 Phenethylamine Sigma-Aldrich 241008 Tyramine Sigma-Aldrich T90344 Dopamine Sigma-Aldrich H8502 Histamine Sigma-Aldrich H7129 Acetylcholine Sigma-Aldrich A2661 L-DOPA Sigma-Aldrich D9628 Succinate Sigma-Aldrich 398055 Serotonin Sigma-Aldrich 14927 Gastrin Sigma-Aldrich G9145 Peptide YY Anaspec AS024401 Pancreatic polypeptide Anaspec AS-22866 Cholecystokinin Sigma-Aldrich C2175 Trace mineral supplement ATCC MD-TMS Vitamin supplement ATCC MD-VS L-glycine Sigma-Aldrich G8898 L-valine Sigma-Aldrich 94619 L-leucine Sigma-Aldrich L8000 L-isoleucine Sigma-Aldrich I2752 L-methionine Sigma-Aldrich 64319 L-histidine Sigma-Aldrich H8000 L-arginine Sigma-Aldrich A5131 L-phenylalanine Sigma-Aldrich P2126 L-tyrosine Sigma-Aldrich T3754 L-tryptophan Sigma-Aldrich T0254 N-methylphenethylamine Sigma-Aldrich M68423 Octopamine Sigma-Aldrich O0250 Synephrine Sigma-Aldrich S0752 Epinephrine Sigma-Aldrich E4250 Norepinephrine Sigma-Aldrich A7257 3-Methoxytyramine Sigma-Aldrich M4251 DMEM Sigma-Aldrich D6429 RPMI 1640 Thermo Fisher 21870092

Example 1. Testing of PRESTO-Salsa

To facilitate pooling of GPCR-barcode pairs, an Expi 293T cell line was generated that stably expresses the Barr-TEV fusion protein. These cells can be efficiently co-transfected with a plasmid that encodes a given GPCR-tTA fusion and a reporter plasmid that encodes a defined barcode downstream of a tet-responsive element. To enable concurrent screening of all GPCRs, the stable Expi 293T cell line was co-transfected with all 314 defined GPCR-barcode reporter plasmid pairs in individual wells in 96 well plates. After about 24 hours, all cells were mixed together in a single tube before the cellular mixtures were redistributed into approximately four 96 well plates. Importantly, Expi 293T cells are non-adherent, which facilitates pooling and redistribution of cells.

After redistribution, each well was stimulated with a given metabolite mixture before harvesting RNA, synthesizing cDNA and then sequencing barcodes via Illumina amplicon sequencing. Relative expression levels of a given barcode were compared to control samples so as to represent the relative activation of a given GPCR by that metabolite or metabolite mixture. To assess the sensitivity and specificity of PRESTO-Salsa, the inventors first established this system using well-known GPCR ligands or microbial metabolite mixtures that contain known GPCR agonists, as shown in FIG. 2 .

These data demonstrate the specificity and sensitivity of the PRESTO-Salsa technology. Importantly, the sequencing-based readout used for PRESTO-Salsa was considerably more sensitive than PRESTO-Tango given the relative sensitivity of sequencing versus luciferase expression.

Example 2. A Forward Chemical Genetic Screen Identifies Human Gut Microbes that Activate GPCRs

144 unique human gut bacteria spanning five phyla, nine classes, eleven orders, and twenty families were isolated from 11 inflammatory bowel disease patients via high-throughput anaerobic culturomics and massively barcoded 16S rRNA gene sequencing. All strains were cultured in gut microbiota medium (Goodman et al., 2011) or Gifu broth at 37° C. under anaerobic conditions and the identities of all strains were confirmed by 16S rRNA gene sequencing. The isolated human gut bacteria are listed in Table 1. Each isolate was grown in monoculture in a medium specialized for the cultivation of human gut microbes (gut microbiota medium; GMM).

Supernatants from individual bacterial monocultures were screened against the near-complete non-olfactory GPCRome (314 conventional GPCRs) using the high-throughput assay Parallel Receptor-ome Expression and Screening via Transcriptional Output-Tango (PRESTO-Tango).

The PRESTO-Tango assay was conducted as follows. HTLA cells, a HEK293 cell line that stably expresses β-arrestin-TEV and tTA-Luciferase (a kind gift from Gilad Barnea, Brown University), were plated in 96-well tissue culture plates (Eppendorf) in DMEM containing 10% FBS and 1% Penicillin/Streptomycin. One day after plating (after reaching approximately 90% confluence) Tango plasmids were transfected into HTLA cells using polyethylenimine (Polysciences). 16-24 hours after transfection, medium was replaced with 180 μl fresh DMEM containing 1% Penicillin/Streptomycin and 10 mM HEPES and 20 μl bacterial medium alone or bacterial supernatant. For PRESTO-Tango screening, all commensals were cultured in gut microbiota medium or Gifu broth for 2 days in an anaerobic chamber (Coy). Commensal supernatants were sterilized by high-speed centrifugation followed by sterile filtration (0.22 μm). For in vitro studies, M. morganii was cultured in minimal medium (MM), or MM with 10 mM L-Phe, 2.5 mM L-Tyr, 10 mM L-DOPA, or 10 mM L-His for 24 hours. Bacterial supernatants were analyzed by LC-MS.

16-24 hours after stimulation, 50 ul per well of Bright-Glo solution (Promega) diluted 20-fold with PBS containing 20 mM HEPES was added into each well. After 20 min incubation at room temperature, luminescence was quantified using a Spectramax i3x (Molecular Devices). Activation fold for each sample was calculated by dividing relative luminescence units (RLU) for each condition by RLUs from media alone controls.

GPCR activation by metabolomes from 144 bacterial strains isolated from the human gut microbiota as measured by PRESTO-Tango. Data is displayed as a heatmap (FIG. 3 ) or on a hierarchical tree of GPCRs organized by class, ligand type, and receptor family (FIG. 4 ). Shading intensity represents the maximum magnitude of activation (log 2) over background across the entire data set—i.e., the maximum activation of a given GPCR by any microbial metabolome in our collection. Radii of the circles at each tip are scaled based on the number of strains that activated a given receptor or receptor family (i.e., number of hits across the complete data set). Hits are defined as activation of a given receptor more than two-fold over background. Graphics were generated in collaboration with Visavisllc using d3.js.

TABLE 1 No. Family Genus Species 1 Bacteroidaceae Bacteroides fragilis 2 Enterobacteriaceae Other Other 3 Streptococcaceae Streptococcus luteciae 4 Clostridiaceae Clostridium perfringens 5 Fusobacteriaceae Fusobacterium spp. 6 Streptococcaceae Streptococcus spp. 7 Enterobacteriaceae UC UC 8 Enterobacteriaceae UC UC 9 Bacteroidaceae Bacteroides fragilis 10 Enterobacteriaceae UC UC 11 Veillonellaceae Megasphaera spp. 12 Clostridiaceae Clostridium perfringens 13 Bacteroidaceae Bacteroides fragilis 14 Bifidobacteriaceae Bifidobacterium UC 15 Bacteroidaceae Bacteroides fragilis 16 Enterobacteriaceae 17 Enterobacteriaceae Morganella spp. 18 Bacteroidaceae Bacteroides spp. 19 Bacteroidaceae Bacteroides ovatus 20 Bifidobacteriaceae Bifidobacterium UC 21 Bifidobacteriaceae Bifidobacterium UC 22 Bacteroidaceae Bacteroides fragilis 23 Enterobacteriaceae UC UC 24 Fusobacteriaceae Fusobacterium spp. 25 Actinobacteria Bifidobacterium adolescentis 26 Actinobacteria Bifidobacterium adolescentis 27 Enterobacteriaceae UC UC 28 Enterobacteriaceae Morganella spp. 29 Enterobacteriaceae UC UC 30 Alcaligenaceae Sutterella spp. 31 Enterobacteriaceae UC UC 32 Bacteroidaceae Bacteroides spp. 33 Enterobacteriaceae UC UC 34 Bacteroidaceae Bacteroides spp. 35 Bacteroidaceae Bacteroides fragilis 36 Actinobacteria Bifidobacterium adolescentis 37 Lactobacillaceae Lactobacillus UC 38 Lactobacillaceae Pediococcus UC 39 Lactobacillaceae Pediococcus UC 40 Enterobacteriaceae UC UC 41 Bacteroidaceae Bacteroides fragilis 42 Bifidobacteriaceae Bifidobacterium spp. 43 Bifidobacteriaceae Bifidobacterium UC 44 Enterobacteriaceae Morganella spp. 45 Streptococcaceae Streptococcus spp. 46 Erysipelotrichales Erysipelotrichaceae spp. 47 Erysipelotrichales Erysipelotrichaceae spp. 48 Clostridiaceae Clostridium perfringens 49 Bifidobacteriaceae Bifidobacterium UC 50 Erysipelotrichales Erysipelotrichaceae spp. 51 Coriobacteriaceae Collinsella aerofaciens 52 Enterobacteriaceae UC UC 53 Coriobacteriaceae Collinsella aerofaciens 54 Clostridiaceae Clostridium perfringens 55 Streptococcaceae Streptococcus spp. 56 Bacteroidaceae Bacteroides ovatus 57 Bacteroidaceae Bacteroides spp. 58 Bacteroidaceae Bacteroides fragilis 59 Bacteroidaceae Bacteroides UC 60 Enterobacteriaceae UC UC 61 Enterobacteriaceae UC UC 62 Bacteroidaceae Bacteroides fragilis 63 Enterobacteriaceae UC UC 64 Porphyromonadaceae Parabacteroides distasonis 65 Clostridiaceae Clostridium perfringens 66 Enterobacteriaceae Morganella spp. 67 Enterobacteriaceae UC UC 68 [Tissierellaceae] Peptoniphilus spp. 69 Enterobacteriaceae Morganella spp. 70 [Tissierellaceae] Peptoniphilus spp. 71 Enterobacteriaceae UC UC 72 Enterobacteriaceae UC UC 73 Coriobacteriaceae Collinsella aerofaciens 74 Lachnospiraceae [Ruminococcus] gnavus 75 Enterobacteriaceae UC UC 76 Lachnospiraceae [Ruminococcus] gnavus 77 Enterobacteriaceae UC UC 78 Enterobacteriaceae UC UC 79 Enterobacteriaceae UC UC 80 Bacteroidaceae Bacteroides spp. 81 Bacteroidaceae Bacteroides uniformis 82 Bacteroidaceae Bacteroides ovatus 83 Coriobacteriaceae Collinsella stercoris 84 Coriobacteriaceae Collinsella stercoris 85 Clostridiales (order) UC UC 86 Lachnospiraceae UC UC 87 Coriobacteriaceae Collinsella stercoris 88 Lactobacillaceae Lactobacillus reuteri 89 Lactobacillaceae Lactobacillus reuteri 90 Bacteroidaceae Bacteroides uniformis 91 Bacteroidaceae Bacteroides UC 92 Sinobacteriaceae Nevskia spp. 93 Lactobacillaceae Lactobacillus reuteri 94 Lactobacillaceae Lactobacillus reuteri 95 Sinobacteriaceae Nevskia spp. 96 Veillonellaceae Acidaminococcus spp. 97 Bacteroidaceae Bacteroides uniformis 98 Bacteroidaceae Bacteroides uniformis 99 Bifidobacteriaceae Bifidobacterium UC 100 Veillonellaceae Acidaminococcus spp. 101 Veillonellaceae Acidaminococcus spp. 102 Bacteroidaceae Bacteroides UC 103 Veillonellaceae Acidaminococcus spp. 104 Veillonellaceae Acidaminococcus spp. 105 Bifidobacteriaceae Bifidobacterium UC 106 Bifidobacteriaceae Bifidobacterium UC 107 Bifidobacteriaceae Bifidobacterium UC 108 Bifidobacteriaceae Bifidobacterium UC 109 Bacteroidaceae Bacteroides uniformis 110 Bacteroidaceae Bacteroides ovatus 111 Veillonellaceae Megasphaera spp. 112 Lachnospiraceae [Ruminococcus] gnavus 113 Lactobacillaceae Pediococcus UC 114 Lactobacillaceae Pediococcus UC 115 Porphyromonadaceae Parabacteroides distasonis 116 Coriobacteriaceae Eggerthela lenta 117 Lachnospiraceae Blautia producta 118 Enterobacteriaceae UC UC 119 Ruminococcaceae Faecalibacterium prausnitzii 120 Clostridiaceae Clostridium perfringens 121 Streptococcaceae Streptococcus spp. 122 Coriobacteriaceae Eggerthela lenta 123 Enterobacteriaceae UC UC 124 Enterobacteriaceae UC UC 125 Not yet identified 126 Lachnospiraceae [Ruminococcus] torques 127 Bacteroidaceae Bacteroides uniformis 128 Erysipelotrichaceae Allobaculum spp. 129 Porphyromonadaceae Parabacteroides spp. 130 Coriobacteriaceae Collinsella stercoris 131 Not yet identified 132 [Tissierellaceae] Peptoniphilus spp. 133 Bacteroidaceae Bacteroides fragilis 134 Ruminococcaceae Oscillospira spp. 135 Enterobacteriaceae Morganella spp. 136 Enterobacteriaceae UC UC 137 Coriobacteriaceae Eggerthela lenta 138 Enterobacteriaceae Morganella spp. 139 Ruminococcaceae Oscillospira spp. 140 Ruminococcaceae Oscillospira spp. 141 Mogibacteriaceae Mogibacterium spp. 142 Moraxellaceae Enhydrobacter spp. 143 Not yet identified Not yet identified 144 Enterobacteriaceae UC UC The culture collection can allow for examination of the effects of phylogenetically diverse taxa while also providing insights into potential strain-specific differences between members of the same species.

An analysis of the above cultures indicated that human gut microbes produce compounds that activate both well-characterized and orphan GPCRs. PRESTO-Tango screening revealed a diverse array of hits, including bacterial-derived metabolite mixtures that activated well-characterized GPCRs as well as mixtures that activated orphan receptors. According to the data in FIG. 4 , activation of at least one GPCR from every class by at least one metabolite mixture was observed across the complete data set.

Specific patterns of GPCR activation emerged based on gross phylogeny. Tables 2 and 3 show the degree of receptor activation for each bacteria according to PRESTO-Tango analysis in each of GMM and Gifu media, respectively. For example, most strains belonging to the phyla Bacteroidetes and Proteobacteria potently activated the succinate receptor (Sucr1), while strains belonging to the phyla Firmicutes, Fusobacteria and Actinobacteria largely failed to activate this receptor (Tables 2-3). However, many activation patterns did not correlate with phylogeny, including multiple examples of bacterial strains that exhibited unique GPCR agonist activities despite being assigned to the same species (Tables 2-3).

TABLE 2 PRESTO-Tango with GMM Gut Erysipelo- Erysipelo- Erysipelo- PRESTO-Tango Microbiota trichaceae trichaceae trichaceae Ruminococcus Ruminococcus Ruminococcus Ruminococcus with GMM Medium spp-1# spp-2# spp-3# gnavus-1# gnavus-2# gnavus-3# torques ADCYAP1R1- 0 0.133 −0.060 −0.281 0.720 0.158 −0.167 −0.511 Tango ADORA1-Tango 0 0.189 0.023 −0.504 0.944 0.234 −0.262 −1.023 ADORA2A-Tango 0 0.237 0.244 −0.038 0.195 −0.018 −1.027 −0.672 ADORA2B-Tango 0 0.067 0.144 −0.209 −0.417 −0.329 −0.816 −0.293 ADORA3-Tango 0 0.567 0.503 0.294 0.165 0.092 −0.157 −0.319 ADRA1A-Tango 0 0.249 −0.041 −0.199 0.987 0.021 −0.608 −0.603 ADRA1B-Tango 0 0.020 −0.258 −0.225 0.930 −0.279 −0.229 −1.010 ADRA1D-Tango 0 −0.197 −0.169 −0.381 1.116 0.260 −0.164 −1.274 ADRA2A-Tango 0 0.132 −0.346 0.261 1.525 0.220 −0.395 −0.585 ADRA2B-Tango 0 −0.391 −1.677 −0.475 1.484 0.793 −1.128 −1.455 ADRA2C-Tango 0 0.216 0.122 −0.345 1.862 0.397 −1.163 −0.955 ADRB1-Tango 0 0.049 0.025 −0.033 1.183 0.705 0.040 −0.463 ADRB2-Tango 0 −0.551 −0.249 0.426 1.103 0.959 −0.319 −0.200 ADRB3-Tango 0 0.000 −0.136 0.415 −0.095 0.118 −0.524 0.245 AGTR1-Tango 0 0.216 0.290 0.000 0.747 0.504 −0.360 −0.042 AGTR2-Tango 0 0.038 0.031 0.207 0.974 0.996 −0.026 −0.618 APJ-Tango 0 −0.326 0.110 −0.535 0.730 0.326 −0.526 −1.093 AVPR1A-Tango 0 0.000 −0.494 0.126 0.354 0.253 −0.695 −0.322 AVPR1B-Tango 0 −0.306 0.133 −0.155 0.491 0.031 −0.122 −0.754 AVPR2-Tango 0 −0.065 −0.003 −0.259 0.551 0.115 −0.207 −0.078 BB3-Tango 0 0.132 0.188 −0.321 0.407 −0.157 −0.386 −0.792 BDKBR1-Tango 0 0.032 −0.105 −0.096 0.199 −0.037 −0.344 −0.003 BDKBR2-Tango 0 0.321 0.349 0.259 0.662 0.669 0.408 −0.041 C3AR1-Tango 0 −0.081 −0.279 0.087 0.580 −0.163 0.239 −0.190 C5A-Tango 0 0.327 0.447 0.481 −0.062 0.634 −0.159 −0.787 CALCRb-Tango 0 −0.175 −0.273 0.095 −0.160 −0.320 −0.695 −0.107 CALCRL-Tango 0 −0.287 −0.024 −0.304 0.163 −0.309 −0.554 0.027 CASR-Tango 0 0.053 0.085 −0.367 −0.222 −0.487 −0.643 −0.846 CCKAR-Tango 0 0.182 0.103 0.167 0.233 0.192 −0.061 −0.218 CCKBR-Tango 0 0.018 0.067 −0.268 1.071 0.119 −0.271 −0.241 CCR10-Tango 0 −0.382 −0.588 0.000 0.282 0.131 −0.596 −0.655 CCR2-Tango 0 −0.119 −0.156 0.237 0.234 0.292 −0.292 −0.779 CCR3-Tango 0 0.561 0.393 0.057 0.831 0.975 −0.976 −0.780 CCR4-Tango 0 0.265 0.307 0.354 1.058 0.610 −0.424 −1.056 CCR5-Tango 0 0.070 0.028 0.507 0.384 0.236 −0.572 −0.587 CCR6-Tango 0 0.145 −0.183 0.492 1.732 1.158 −0.410 −0.652 CCR7-Tango 0 0.309 0.404 0.428 1.077 0.255 −0.292 −0.919 CCR8-Tango 0 −0.334 −0.334 −0.680 0.891 0.304 −0.541 −1.416 CCRL2-Tango 0 0.590 0.193 0.679 0.312 −0.257 −0.563 −0.497 CD97-Tango 0 0.170 0.170 −0.606 −0.192 −0.157 −0.235 −0.261 CHRM1-Tango 0 −0.585 −0.260 −0.418 0.588 0.058 −0.712 0.000 CHRM2-Tango 0 0.046 0.002 −0.688 0.791 −0.620 −0.157 0.371 CHRM3-Tango 0 −0.196 −0.140 −0.628 1.554 0.232 −0.310 0.138 CHRM4-Tango 0 −0.209 −0.453 0.214 0.153 0.189 −0.790 0.085 CHRM5-Tango 0 0.340 0.277 0.429 0.371 0.428 −0.349 −0.283 CMKLR1-Tango 0 −0.008 0.015 0.208 0.084 0.436 −0.742 −0.176 CNR1-Tango 0 −0.095 0.110 −0.516 0.436 −0.388 −0.411 −0.751 CNR2-Tango 0 −0.160 −0.217 0.031 0.391 −0.045 −0.812 −0.387 CRHR1-Tango 0 0.396 0.225 −0.293 0.260 0.260 −0.888 −0.585 CRHR2-Tango 0 0.057 0.062 −0.288 0.000 0.129 0.189 −0.334 CX3C1-Tango 0 −0.018 0.272 −0.570 1.107 0.000 0.091 −0.313 CXCR1-Tango 0 0.165 0.144 0.378 −0.015 −0.079 −0.611 −0.365 CXCR2-Tango 0 0.311 −0.079 0.238 0.702 1.533 −0.588 −0.722 CXCR3-Tango 0 0.277 0.476 −0.036 0.722 0.936 −0.344 0.033 CXCR4-Tango 0 0.703 0.705 0.455 0.629 0.697 −0.345 −0.175 CXCR5-Tango 0 0.150 0.534 0.043 0.724 0.115 −0.628 −0.121 CXCR6-Tango 0 −0.335 −0.798 −0.105 0.216 0.181 0.037 −0.186 CXCR7-Tango 0 −0.388 −0.173 −0.559 1.395 0.343 −0.655 −1.188 CYSLTR1-Tango 0 0.036 0.272 0.299 0.950 1.176 −0.389 −1.348 CYSLTR2-Tango 0 0.239 0.081 0.776 0.367 0.149 −1.082 −0.922 DRD1-Tango 0 0.151 −0.116 0.155 0.555 0.134 −0.710 −0.727 DRD2-Tango 0 0.329 0.210 −0.360 0.043 −0.544 −0.735 −1.792 DRD3-Tango 0 0.991 0.193 0.082 0.088 −0.373 −2.273 −1.708 DRD4-Tango 0 1.258 0.685 0.404 0.101 0.000 −2.174 −2.296 DRD5-Tango 0 −0.220 −0.150 −0.053 0.216 −0.078 −0.616 −0.372 EDNRA-Tango 0 0.244 0.226 0.233 0.489 0.137 −0.044 −0.066 EDNRB-Tango 0 0.230 0.268 −0.106 0.270 0.145 −0.598 −0.122 ELTD1-Tango 0 0.280 0.243 −0.374 0.543 0.051 −0.799 −0.957 F2R-Tango 0 0.015 0.100 0.014 0.511 0.189 −0.090 −0.141 F2RL1-Tango 0 0.002 −0.018 −0.055 0.310 0.163 −0.204 −0.314 F2RL2-Tango 0 0.381 0.337 0.326 0.003 −0.284 −0.284 −0.389 F2RL3-Tango 0 −0.025 −0.063 −0.003 0.204 −0.178 −0.345 −0.057 FFA1-Tango 0 0.502 0.430 0.115 0.832 0.550 0.079 −0.199 FFA2-Tango 0 0.631 0.433 −0.083 1.061 0.576 −0.649 −0.614 FFA3-Tango 0 0.345 0.372 0.345 0.736 0.245 −0.141 −0.406 FPR1-Tango 0 1.373 1.299 1.166 0.678 0.356 −0.426 0.386 FPR2-Tango 0 0.800 0.607 0.371 2.058 −0.012 −0.501 −0.316 FPR3-Tango 0 −0.029 −0.029 0.038 0.677 0.418 −0.593 −0.293 FSHR-Tango 0 0.278 0.148 −0.215 −0.084 −0.393 −0.348 −0.464 GABBR1-Tango 0 −0.104 −0.104 −0.091 2.454 −0.163 −0.796 −0.313 GAL1-Tango 0 −0.184 0.000 −0.526 1.319 −0.357 −0.771 −1.186 GAL2-Tango 0 0.165 −0.298 0.054 −0.335 −0.528 −0.458 −0.071 GAL3-Tango 0 −0.465 −0.531 −0.698 0.405 0.100 −0.638 −1.390 GCGR-Tango 0 0.115 0.358 −0.136 0.459 0.112 −0.645 0.140 GHRHR-Tango 0 0.123 0.070 0.173 0.063 0.047 −0.160 0.343 GHSR-Tango 0 0.273 0.261 0.122 0.756 0.580 −0.411 −0.103 GIPR-Tango 0 −0.465 −0.297 0.000 0.178 0.139 −0.691 −0.122 GLP1R-Tango 0 0.023 0.064 −0.351 −0.293 −0.714 −0.442 −0.389 GLP2R-Tango 0 −0.226 −0.079 −0.313 0.208 0.816 0.103 −0.418 GNRHR-Tango 0 −0.087 −0.580 −0.075 −1.062 −0.537 −0.312 −0.191 GPBA-Tango 0 −0.954 −0.248 −0.246 0.877 0.846 −0.993 −0.559 GPER-Tango 0 −0.100 0.131 0.206 0.679 0.180 −0.890 −0.408 GPR1-Tango 0 0.141 0.191 −0.166 0.022 0.014 −0.408 −0.511 GPR101-Tango 0 0.166 −0.029 0.321 0.322 0.392 0.127 −0.319 GPR110-Tango 0 −0.161 −0.046 −0.694 0.274 −0.655 −0.325 −0.895 GPR111-Tango 0 −0.515 −0.441 −0.324 −0.194 −0.523 −0.423 −0.245 GPR113-Tango 0 −0.180 −0.068 −0.331 0.008 0.000 0.133 −0.605 GPR114-Tango 0 −0.200 0.005 −0.211 −0.155 −0.013 −0.415 −0.143 GPR115-Tango 0 0.220 0.220 −0.505 0.094 −0.197 0.168 −0.640 GPR116-Tango 0 −0.027 −0.125 −0.594 0.085 −0.039 −0.400 −0.639 GPR119-Tango 0 −0.107 0.000 −0.428 −0.096 0.202 −0.538 −0.538 GPR12-Tango 0 −0.039 −0.045 −0.265 0.871 0.608 −0.106 −0.009 GPR120-Tango 0 0.160 −0.198 −0.477 1.235 0.360 −0.825 −0.655 GPR123-Tango 0 0.210 0.264 0.366 0.645 0.371 −0.220 −0.296 GPR124-Tango 0 0.337 0.234 −0.114 0.005 0.262 −0.543 −0.681 GPR125-Tango 0 −0.085 −0.004 −0.056 0.069 0.147 −0.572 −1.185 GPR126-Tango 0 −0.013 −0.166 −0.697 0.601 −0.148 −0.538 −0.339 GPR132-Tango 0 −0.239 −0.084 −0.476 0.572 −0.535 −0.418 −0.679 GPR133-Tango 0 0.026 0.132 −0.433 0.358 0.108 −0.141 −0.504 GPR135-Tango 0 −0.286 −0.448 0.181 −0.012 −0.113 −0.252 0.009 GPR141-Tango 0 0.075 0.147 −0.147 1.241 0.288 −0.687 −0.806 GPR142-Tango 0 −0.531 −0.155 −0.119 0.617 0.320 −0.314 −0.769 GPR143-Tango 0 0.383 −0.225 −0.190 0.464 −0.341 −0.553 −0.897 GPR144-Tango 0 0.057 −0.298 −0.341 −0.695 −0.371 −0.534 −0.534 GPR146-Tango 0 0.866 0.718 0.170 −0.931 −0.335 −0.462 −0.632 GPR148-Tango 0 0.093 −0.170 −0.122 −0.160 −0.879 −0.940 −0.658 GPR149-Tango 0 0.515 0.302 0.095 0.149 0.046 −0.354 −0.855 GPR15-Tango 0 0.335 0.335 0.077 1.019 0.197 −0.824 −0.409 GPR150-Tango 0 0.000 0.000 0.000 1.127 0.692 −0.159 0.322 GPR151-Tango 0 0.327 0.197 −0.377 0.534 0.357 −0.086 −0.067 GPR152-Tango 0 −0.071 −0.147 −0.488 0.382 0.173 −0.072 −0.455 GPR153-Tango 0 0.364 0.248 0.204 0.040 0.254 −0.167 −0.318 GPR156-Tango 0 −0.556 −0.556 −0.516 0.916 0.158 −0.078 −0.519 GPR157-Tango 0 0.111 0.141 0.299 0.282 0.108 −0.538 −0.571 GPR158-Tango 0 −0.162 −0.260 −0.898 0.288 0.132 −0.120 −0.470 GPR160-Tango 0 0.367 0.298 −0.139 0.514 0.106 −0.149 −0.451 GPR161-Tango 0 0.323 0.362 0.352 0.365 0.155 −0.672 −0.784 GPR162-Tango 0 −0.045 0.116 −0.048 0.004 −0.142 −0.919 −0.361 GPR17-Tango 0 0.203 0.373 −0.091 0.687 0.747 −0.618 −1.035 GPR171-Tango 0 0.297 0.179 0.085 0.618 0.246 −0.722 −0.788 GPR173-Tango 0 −0.400 −0.140 0.343 0.853 0.133 −0.716 −0.583 GPR174-Tango 0 0.906 0.410 −0.093 0.378 0.509 −0.675 −0.410 GPR18-Tango 0 0.171 0.374 −0.052 0.287 0.182 −0.808 −0.612 GPR182-Tango 0 0.514 0.610 0.327 0.241 0.396 −0.570 −0.804 GPR183-Tango 0 0.548 0.296 −0.011 0.134 0.311 −0.910 −0.176 GPR19-Tango 0 0.386 0.467 0.079 0.228 0.201 −0.409 −0.908 GPR20-Tango 0 1.372 1.000 1.087 0.000 0.243 −0.095 −0.095 GPR21-Tango 0 −0.206 0.229 −0.202 0.133 0.155 −0.327 −0.739 GPR22-Tango 0 0.296 0.072 −0.175 0.423 0.157 −0.467 −0.911 GPR25-Tango 0 0.144 0.207 −0.045 0.040 0.063 −0.404 −0.426 GPR26-Tango 0 0.229 −0.012 0.270 1.000 0.639 0.709 −0.634 GPR27-Tango 0 0.440 −0.020 −0.242 −0.068 0.240 −0.657 −0.168 GPR3-Tango 0 0.484 0.365 −0.250 0.797 0.604 0.079 −0.246 GPR31-Tango 0 0.564 0.717 0.033 0.826 0.392 0.031 −0.454 GPR32-Tango 0 −0.171 −0.171 −0.072 0.733 0.693 −0.289 −0.264 GPR34-Tango 0 0.167 0.233 −0.296 0.644 −0.215 −0.286 −0.129 GPR35-Tango 0 0.126 0.272 −0.053 0.382 0.141 −0.177 −0.198 GPR37-Tango 0 0.379 0.377 0.285 0.122 −0.501 0.041 −0.428 GPR37L1-Tango 0 0.048 0.122 0.035 0.043 −0.145 −0.211 −0.495 GPR39-Tango 0 −0.200 −0.320 −0.269 0.424 −0.046 −0.136 −0.452 GPR4-Tango 0 0.344 0.393 0.036 −0.167 −0.433 0.265 −0.122 GPR44-Tango 0 −0.221 −0.148 0.000 0.033 −0.652 −0.388 −0.027 GPR45-Tango 0 0.000 −0.144 −0.506 0.665 0.900 −0.534 −0.299 GPR50-Tango 0 0.345 0.221 0.544 0.525 0.259 −0.104 −0.361 GPR52-Tango 0 −0.189 −0.274 0.051 1.098 0.124 0.010 −0.383 GPR55-Tango 0 0.554 0.079 −0.081 0.549 −0.196 −0.241 0.016 GPR56-Tango 0 0.442 0.344 −0.333 0.736 −0.294 −0.345 0.081 GPR6-Tango 0 0.528 0.402 0.220 0.651 0.367 −0.551 −0.093 GPR61-Tango 0 0.178 −0.011 0.253 0.230 0.044 0.194 0.047 GPR62-Tango 0 0.264 0.000 0.140 0.350 0.030 −0.162 0.194 GPR63-Tango 0 −0.362 −0.148 −0.153 0.845 0.239 −0.493 −0.814 GPR64-Tango 0 −0.261 −0.414 −0.287 0.407 0.020 −0.251 −0.282 GPR65-Tango 0 −0.035 −0.044 −0.068 0.246 0.324 −0.092 −0.302 GPR68-Tango 0 −0.097 −0.004 −0.256 −0.045 −0.379 −0.274 −0.379 GPR75-Tango 0 0.024 −0.114 −0.073 0.637 0.628 −0.341 −0.585 GPR77-Tango 0 −0.354 −0.506 −0.010 0.524 0.351 −0.257 −0.459 GPR78-Tango 0 0.373 0.195 0.262 0.534 0.449 −0.011 −0.288 GPR82-Tango 0 0.187 0.078 −0.043 0.413 0.706 −0.046 −0.529 GPR83-Tango 0 0.118 0.186 −0.031 1.101 0.147 −0.145 −0.494 GPR84-Tango 0 0.350 0.367 0.172 0.798 0.636 −0.265 −0.825 GPR85-Tango 0 0.357 0.206 0.016 0.651 0.500 −0.450 −0.455 GPR87-Tango 0 0.388 0.372 0.357 0.546 1.022 −0.168 −0.565 GPR88-Tango 0 −0.150 −0.039 0.077 0.492 0.138 −0.388 −0.245 GPR97-Tango 0 0.134 −0.308 −0.118 0.822 0.436 −0.411 −0.832 GPRC5A-Tango 0 0.501 0.429 0.285 0.814 0.603 −0.311 −0.552 GPRC5B-Tango 0 0.397 0.293 0.082 0.417 0.389 −0.338 −0.918 GPRC5C-Tango 0 0.475 0.504 0.298 0.976 0.351 −0.643 −0.949 GPRC5D-Tango 0 −0.278 −0.364 −0.223 1.037 0.173 −0.314 −0.545 GPRC6A-Tango 0 −0.272 −0.397 −0.805 −0.054 −0.486 −0.137 −0.713 GRM1-Tango 0 −0.016 −0.191 −0.457 0.374 −0.012 −0.466 −0.615 GRM2-Tango 0 −0.134 −0.125 −0.628 0.769 −0.749 −0.244 −0.807 GRM4-Tango 0 0.276 0.091 0.128 0.280 −0.759 −0.147 −0.818 GRM5-Tango 0 −0.558 −0.484 −0.564 −0.047 −0.449 −0.056 −0.386 GRM6-Tango 0 −0.545 −0.717 −0.157 −0.045 −0.230 −0.531 0.098 GRM7-Tango 0 −0.194 −0.443 −1.066 −0.409 −0.302 −0.268 −0.945 GRM8-Tango 0 −0.434 −0.406 −0.435 0.800 −0.806 −0.631 −0.689 GRPR-Tango 0 0.145 0.016 0.028 0.417 −0.260 −0.345 −0.620 HCA1-Tango 0 0.409 0.892 0.357 0.846 0.569 −0.871 −0.494 HCA2-Tango 0 0.485 0.385 0.396 0.851 0.941 −0.652 −1.250 HCA3-Tango 0 0.597 0.977 −0.706 0.751 0.666 −0.874 −0.738 HCTR1-Tango 0 0.600 0.760 −0.160 0.867 1.270 −0.407 −0.623 HCTR2-Tango 0 −0.160 0.317 0.112 0.511 0.766 0.000 −0.778 HRH1-Tango 0 0.258 0.336 0.266 0.115 0.841 −0.874 −1.459 HRH2-Tango 0 0.235 0.609 0.108 0.185 0.408 −0.906 −0.391 HRH3-Tango 0 −0.372 −0.481 −0.366 0.212 1.205 −1.464 −1.324 HRH4-Tango 0 0.081 0.224 −0.111 0.325 1.156 −0.658 −0.949 HTR1A-Tango 0 0.772 0.777 0.250 1.765 0.370 −0.597 −0.295 HTR1B-Tango 0 0.647 0.697 0.301 0.728 −0.005 −0.359 −0.824 HTR1D-Tango 0 0.609 0.736 0.101 0.899 0.589 −0.961 −0.503 HTR1E-Tango 0 0.582 0.744 0.458 0.690 0.659 −0.056 −0.853 HTR1F-Tango 0 0.384 0.591 0.208 1.009 0.451 −0.261 −0.838 HTR2A-Tango 0 0.109 −0.063 0.306 0.856 −0.264 −0.791 −0.694 HTR2B-Tango 0 0.351 0.437 −0.094 −0.149 −0.613 −0.112 −0.889 HTR2C-Tango 0 −0.196 −0.299 0.093 0.983 −0.140 −0.908 −0.792 HTR4-Tango 0 0.419 0.349 0.628 1.408 0.558 −0.445 −0.020 HTR5-Tango 0 0.531 0.481 0.788 0.899 1.026 0.317 −0.238 HTR6-Tango 0 0.308 0.000 0.354 1.137 −0.199 −0.707 −1.368 HTR7-Tango 0 0.354 0.002 0.501 1.127 −0.400 −0.376 −0.775 KISSPEPTIN-R- 0 −0.292 0.050 0.369 0.187 −0.350 −0.538 −0.066 Tango LHCGR-Tango 0 0.231 0.088 −0.422 0.520 0.083 −0.457 −0.989 LPAR1-Tango 0 0.539 0.630 0.407 1.019 0.359 0.040 −0.836 LPAR2-Tango 0 0.069 −0.226 −0.107 0.314 0.166 −0.359 −0.455 LPAR4-Tango 0 0.369 0.175 0.618 0.867 0.538 −0.480 −1.139 LPAR5-Tango 0 −0.079 0.282 0.166 1.396 −0.043 −0.716 −1.064 LPAR6-Tango 0 0.244 −0.057 0.480 0.614 0.220 −0.445 −1.739 LTB4R-Tango 0 0.023 −0.124 0.355 0.616 0.260 −0.755 −1.317 LTB4R2B-Tango 0 0.442 0.475 0.110 0.089 0.544 −0.275 −0.696 MAS1-Tango 0 0.088 0.239 −0.291 0.415 0.088 −0.109 −0.234 MAS1L-Tango 0 −0.131 −0.231 −0.184 −0.338 −0.207 −0.204 −1.013 MC1R-Tango 0 −0.304 0.095 −0.135 −0.230 −0.280 −0.897 −0.484 MC2R-Tango 0 0.151 −0.019 0.600 −0.221 0.184 −0.255 −0.627 MC3R-Tango 0 0.331 0.308 0.140 0.916 −0.097 −0.862 −1.611 MC4R-Tango 0 1.031 0.606 0.511 1.212 0.873 −0.188 −0.490 MC5R-Tango 0 0.576 0.564 0.622 1.049 0.819 0.320 −0.385 MCHR1-Tango 0 −1.293 −0.929 −0.199 0.971 0.051 −0.276 −1.436 MCHR2-Tango 0 −0.413 −0.436 0.189 −0.075 −0.288 −0.569 −1.196 MLNR-Tango 0 −0.304 −0.156 0.049 0.000 −0.429 −0.173 −0.077 MRGPRD-Tango 0 −0.049 0.116 0.059 0.446 0.157 −0.407 −1.314 MRGPRE-Tango 0 −0.370 −0.271 −0.080 0.649 0.186 −0.406 −0.489 MRGPRF-Tango 0 0.111 0.302 0.297 1.102 0.860 −0.111 −1.202 MRGPRG-Tango 0 −0.556 −0.738 −0.263 −0.253 −0.343 −0.382 −1.061 MRGPRX1-Tango 0 −0.681 −0.633 −0.141 1.284 0.075 −1.435 −1.460 MRGPRX2-Tango 0 −0.237 0.050 0.374 0.458 −0.182 −0.807 −1.871 MRGPRX3-Tango 0 0.074 0.131 0.378 0.193 −0.222 −0.596 −1.101 MRGPRX4-Tango 0 −0.365 0.045 −0.293 0.678 −0.520 −0.984 −1.173 MTNR1A-Tango 0 0.440 0.339 −0.060 0.730 0.610 0.125 −0.845 MTNR1B-Tango 0 −0.076 0.014 −0.009 2.155 0.753 −0.150 −1.114 NMBR-Tango 0 −0.261 −0.274 0.600 0.800 0.579 0.063 −0.664 NMUR1-Tango 0 0.591 0.528 −0.252 0.827 0.711 0.000 −0.160 NMUR2-Tango 0 0.878 1.141 0.528 −0.070 0.436 −0.771 −0.037 NPBW1-Tango 0 0.731 0.880 −0.243 0.494 −0.055 −0.935 −0.267 NPBW2-Tango 0 0.727 0.788 −0.807 1.290 0.218 −0.382 −1.199 NPFF1-Tango 0 −0.848 −1.016 −0.128 −0.186 0.000 −1.467 −0.800 NPFF2-Tango 0 0.586 0.445 −0.146 0.227 0.184 −0.141 −0.693 NPS-Tango 0 −0.576 −0.351 0.067 0.396 0.551 −0.616 −0.915 NPY1R-Tango 0 −0.094 −0.076 0.051 0.650 0.804 −0.577 −0.830 NPY2R-Tango 0 −0.223 0.098 −0.032 0.206 −0.047 −0.188 −0.761 NPY4R-Tango 0 0.060 0.218 −0.077 −0.202 −0.058 −0.176 0.035 NPY5R-Tango 0 0.327 0.184 −0.424 0.981 0.105 −0.597 −1.037 NTSR1-Tango 0 0.201 0.335 −0.583 0.003 0.360 −0.382 −0.755 NTSR2-Tango 0 0.188 0.690 0.112 0.695 0.358 −0.883 −0.781 OPN3-Tango 0 0.136 0.213 0.097 0.137 0.156 −0.435 0.015 OPN5-Tango 0 0.736 0.463 −0.846 1.310 0.604 −0.692 −1.177 OPRD1-Tango 0 −0.026 −0.065 −0.636 0.883 0.414 −1.290 −0.707 OPRK1-Tango 0 −0.404 −0.580 0.407 0.161 0.323 −0.397 −1.041 OPRL1-Tango 0 −0.693 −0.259 0.291 0.000 −0.127 −0.071 −0.252 OPRM1-Tango 0 0.067 0.115 0.063 1.062 0.097 −0.445 −0.082 OXER1-Tango 0 −0.292 −0.356 −0.020 −0.102 −0.499 −0.217 −0.334 OXGR1-Tango 0 −0.097 0.159 −0.426 0.940 1.218 −0.645 −0.578 OXTR-Tango 0 0.395 0.384 0.341 0.042 −0.450 −0.821 −0.045 P2RY1-Tango 0 0.418 0.569 −0.780 0.396 −0.051 −0.666 −1.328 P2RY10-Tango 0 0.118 0.333 −0.442 0.587 0.230 −0.639 −0.598 P2RY11-Tango 0 0.192 0.276 −0.645 0.415 0.000 −0.576 −0.542 P2RY12-Tango 0 0.355 0.355 0.223 0.929 0.213 −0.724 −0.519 P2RY13-Tango 0 0.250 0.411 0.121 0.723 0.590 −0.154 −0.549 P2RY14-Tango 0 −0.065 −0.200 −0.163 0.162 −0.215 −0.366 −0.893 P2RY2-Tango 0 0.199 −0.022 −0.551 0.235 −0.512 −0.585 −0.801 P2RY4-Tango 0 0.404 0.355 −0.071 0.218 −0.126 −0.692 −0.649 P2RY6-Tango 0 −0.077 −0.095 −0.277 0.216 0.131 −0.447 −0.829 P2RY8-Tango 0 −0.237 −0.369 −0.409 0.480 −0.069 −0.292 −0.470 PK1-Tango 0 −0.397 −0.224 0.368 0.284 −0.115 −0.709 −0.495 PK2-Tango 0 0.181 −0.357 −0.317 0.565 −0.030 −0.181 −0.100 PRRP-Tango 0 0.447 0.386 −0.241 0.621 0.746 −0.089 −0.202 PTAFR-Tango 0 0.381 0.350 −0.432 0.149 −0.278 −0.649 −1.153 PTGDR-Tango 0 −0.095 0.168 −0.236 0.662 −0.015 −1.002 −0.776 PTGER1-Tango 0 0.427 0.188 0.342 0.542 0.115 −0.523 −0.118 PTGER2-Tango 0 0.000 0.292 −0.077 0.547 0.458 −0.050 −0.193 PTGER3-Tango 0 0.552 0.242 0.135 1.344 0.621 −0.330 −0.882 PTGER4-Tango 0 0.546 0.073 0.650 0.546 0.367 −0.532 −1.022 PTGFR-Tango 0 0.646 0.305 −0.200 0.047 0.580 −0.458 −0.730 PTGIR-Tango 0 0.363 0.628 0.048 0.877 0.593 −1.498 −1.394 PTH1R-Tango 0 0.160 −0.026 −0.018 1.093 0.109 −0.280 −0.692 PTH2R-Tango 0 0.136 0.028 −0.525 0.720 0.185 −0.343 −1.165 QRFP-Tango 0 0.234 0.000 −0.220 −0.175 −0.092 −0.619 −0.549 RXFP1-Tango 0 −0.419 −0.157 −0.514 0.022 0.022 −0.754 −1.277 RXFP2-Tango 0 −0.060 −0.097 −0.651 −0.190 −0.695 −0.300 −1.033 RXFP3-Tango 0 −0.046 −0.159 −0.283 0.101 0.548 −0.688 −0.479 RXFP4-Tango 0 0.507 −0.165 −0.122 −0.054 0.315 −0.156 −0.705 S1PR1-Tango 0 0.707 0.622 −0.355 1.253 0.818 −0.033 −0.937 S1PR2-Tango 0 0.648 0.747 0.323 0.930 0.234 −0.531 −1.529 S1PR3-Tango 0 0.560 0.624 0.059 0.295 0.295 −0.018 −0.276 S1PR4-Tango 0 0.397 0.307 −0.644 1.331 1.054 −0.762 −1.014 S1PR5-Tango 0 0.627 0.720 −0.707 0.754 1.064 −0.627 −0.258 SCTR-Tango 0 0.370 0.260 0.146 0.297 0.225 −0.685 −0.563 SSTR1-Tango 0 0.285 −0.142 0.103 0.591 0.145 0.127 −0.189 SSTR2-Tango 0 0.081 0.428 0.591 0.830 0.395 −0.181 −0.878 SSTR3-Tango 0 0.745 −0.062 0.445 0.372 0.878 −0.819 −0.415 SSTR4-Tango 0 0.108 0.292 0.293 0.853 0.690 −1.267 −0.756 SSTR5-Tango 0 0.385 −0.165 0.754 0.771 1.234 0.213 −0.310 SUCNR1-Tango 0 −0.546 −0.117 −0.120 1.408 3.755 −0.798 −0.646 TA1-Tango 0 0.272 0.060 −0.114 0.761 −0.057 −0.362 −0.981 TAAR2-Tango 0 −0.107 0.017 −0.455 0.147 0.321 −0.414 −1.027 TAAR5-Tango 0 −0.716 −0.756 −0.066 −0.141 −0.208 −0.939 −0.130 TAAR6-Tango 0 −0.424 −0.716 −0.010 −0.188 −0.290 −0.030 −1.116 TAAR8-Tango 0 −0.205 −0.465 −0.205 0.176 0.317 0.064 −0.361 TAAR9-Tango 0 −0.192 −0.365 −0.129 −0.176 −0.288 −0.110 −0.464 TACR1-Tango 0 −0.064 −0.241 −0.240 0.140 −0.422 −0.085 −1.207 TACR2-Tango 0 0.204 0.155 −0.021 0.495 0.262 −0.965 −0.948 TACR3-Tango 0 0.435 0.427 0.067 0.841 0.503 −0.943 −1.376 TBXA2R-Tango 0 0.150 −0.022 0.087 0.019 −0.218 −0.742 −0.315 TSHR-Tango 0 −0.378 −0.294 −0.423 −0.184 −0.703 0.004 −0.996 UTS2R-Tango 0 −0.065 0.194 0.000 0.719 0.410 −0.260 −0.260 VIPR1-Tango 0 −0.920 −1.130 0.184 0.939 0.191 −0.759 −1.689 VIPR2-Tango 0 0.829 0.803 0.250 0.050 0.220 −1.527 −1.293 PRESTO-Tango Blautia Faecalibacterium Oscillospira Oscillospira (Lachnospiraceae) (Clostridiales) Lactobacillus Lactobacillus with GMM producta prausnitzii spp-1# spp-2# UC/UC UC/UC reuteri-1# reuteri-2# ADCYAP1R1- 0.246 −0.297 −0.210 0.017 −0.135 −1.152 −0.232 −0.442 Tango ADORA1-Tango 0.026 −0.475 0.220 0.261 −0.839 −1.784 −1.125 −0.934 ADORA2A-Tango −0.081 −0.152 −0.227 −0.095 −0.877 −1.168 −0.762 −1.398 ADORA2B-Tango −0.138 0.068 −0.145 −0.339 −0.417 −0.978 −0.675 −0.831 ADORA3-Tango 0.321 0.112 −0.178 −0.252 0.219 −0.473 −0.342 −0.150 ADRA1A-Tango −0.117 −0.127 −0.410 −0.347 −0.239 −0.900 −0.427 −0.771 ADRA1B-Tango −0.459 −0.990 −0.161 −0.174 −0.473 −0.753 −0.304 −0.597 ADRA1D-Tango −0.621 −1.196 −0.490 −1.037 0.033 −0.410 −0.244 −0.666 ADRA2A-Tango 0.321 0.196 −0.145 0.247 −0.727 −2.201 −1.535 −1.365 ADRA2B-Tango −1.241 −0.553 −0.621 −0.593 −1.064 −2.262 −1.103 −1.038 ADRA2C-Tango −0.347 −0.163 0.427 0.261 −0.525 −1.158 −0.525 −1.550 ADRB1-Tango 0.323 −0.055 0.068 0.853 −0.705 −0.588 −0.569 −0.786 ADRB2-Tango −0.948 −0.160 −0.263 −0.196 0.000 −1.019 0.078 0.000 ADRB3-Tango −0.374 0.127 0.242 0.025 −0.467 −0.394 −0.405 −0.288 AGTR1-Tango −0.307 −0.012 0.312 0.383 −0.302 −0.952 −0.302 −0.045 AGTR2-Tango 0.392 −0.310 0.175 0.371 0.150 −0.636 0.088 0.271 APJ-Tango 0.488 0.341 −0.552 −1.275 0.071 −0.957 −0.294 −0.258 AVPR1A-Tango −0.695 0.614 −0.328 −1.002 −0.082 −1.492 −0.285 −0.317 AVPR1B-Tango −0.078 −0.027 0.248 −0.239 −0.734 −0.731 −0.716 −1.276 AVPR2-Tango −0.438 0.202 −0.109 −0.377 −0.918 −1.335 −1.474 −1.014 BB3-Tango −0.451 −0.469 −0.351 −0.083 −1.141 −0.987 −1.534 −1.472 BDKBR1-Tango 0.107 0.004 0.021 0.225 −0.822 −0.581 −1.186 −0.926 BDKBR2-Tango 0.827 0.591 −0.161 0.009 0.284 −0.699 −0.210 −0.033 C3AR1-Tango 0.289 0.395 −0.427 −0.332 −0.703 −0.852 −0.717 −0.505 C5A-Tango 0.579 −0.201 0.103 −0.370 0.731 −0.324 −0.350 −0.304 CALCRb-Tango 0.036 0.065 −0.254 −0.619 −0.090 −0.676 −0.160 −0.786 CALCRL-Tango −0.311 −0.037 −0.190 −0.240 −0.221 −0.455 −0.221 −0.359 CASR-Tango −0.527 −0.674 0.036 −0.398 −0.403 −0.168 −0.543 −0.843 CCKAR-Tango 0.241 −0.094 0.025 −0.205 −0.314 −0.409 −0.181 −0.237 CCKBR-Tango −0.384 −0.200 −0.081 −0.486 −0.424 −0.741 −0.404 −0.273 CCR10-Tango −1.073 −0.102 −0.251 −0.935 −0.567 −1.023 −0.807 −0.653 CCR2-Tango −0.171 0.086 −0.577 −1.262 −0.071 −0.899 −0.756 −0.411 CCR3-Tango −0.341 −0.476 −0.455 −1.046 0.467 −1.613 −0.217 0.000 CCR4-Tango −0.216 −0.666 −0.563 −0.818 0.234 −0.650 −0.074 −0.138 CCR5-Tango 0.109 −0.110 0.000 −0.593 0.226 −0.315 0.154 −0.364 CCR6-Tango 0.174 0.153 −0.435 −0.210 1.029 −0.927 0.372 0.143 CCR7-Tango −0.091 −0.595 −0.713 −1.033 −0.249 −0.398 −0.385 −0.595 CCR8-Tango −0.640 −0.575 −0.481 −1.042 −0.126 −0.676 −0.626 −0.664 CCRL2-Tango 0.437 −0.318 0.350 0.556 −0.363 −1.189 −0.327 −0.463 CD97-Tango −0.235 −0.300 0.680 1.462 −0.569 −1.190 −0.785 −0.728 CHRM1-Tango −0.110 −0.023 0.503 0.364 −0.485 −0.995 −1.177 −0.645 CHRM2-Tango −0.140 −0.188 0.395 0.829 −0.912 −0.827 −1.405 −1.429 CHRM3-Tango −0.913 −0.747 0.226 0.321 −0.794 −0.838 −0.733 −1.159 CHRM4-Tango 0.000 0.031 0.052 0.437 −0.447 −0.333 −0.779 −0.619 CHRM5-Tango 0.664 −0.173 −0.131 0.281 −0.162 −0.158 −0.495 −0.559 CMKLR1-Tango 0.438 0.458 −0.079 0.114 −0.384 −0.411 −0.360 −0.042 CNR1-Tango −0.350 −0.284 −0.081 −0.565 −0.242 −0.180 −0.515 −1.137 CNR2-Tango 0.000 −0.116 −0.333 −0.464 −0.239 −0.628 −0.392 −0.072 CRHR1-Tango −0.491 −0.491 −0.257 0.357 −0.196 −0.727 0.260 −0.477 CRHR2-Tango 0.109 −0.024 −0.153 −0.484 −0.141 −0.359 −0.674 −0.925 CX3C1-Tango 0.288 0.733 −0.781 −0.728 −0.674 −0.620 −1.019 −0.769 CXCR1-Tango −0.611 −0.280 0.212 −0.104 −0.777 −0.857 −0.906 −0.258 CXCR2-Tango −0.235 −0.462 −0.264 −0.023 −0.369 −0.429 −0.360 −0.203 CXCR3-Tango −0.013 0.149 0.111 0.393 −0.131 −0.914 −0.293 −0.169 CXCR4-Tango 0.694 −0.009 0.295 0.695 −0.007 −0.555 −0.553 −0.231 CXCR5-Tango 0.409 0.172 0.128 0.409 −0.310 −0.671 −0.263 −0.276 CXCR6-Tango 0.371 −0.103 0.287 −0.153 −0.534 −0.371 −0.748 −0.784 CXCR7-Tango −0.822 −0.793 0.204 −0.761 −0.637 −1.037 −1.009 −0.809 CYSLTR1-Tango −1.111 −0.726 −0.191 −0.735 −0.290 −1.067 −0.637 −0.364 CYSLTR2-Tango −0.778 −0.748 0.568 −0.189 −0.742 −0.826 −0.508 −0.485 DRD1-Tango −0.649 −0.212 0.280 0.280 −0.215 −1.164 −0.689 −0.689 DRD2-Tango 1.803 −0.562 0.216 0.144 −1.570 −1.259 −1.932 −1.357 DRD3-Tango 0.610 −0.836 2.161 1.938 −2.857 −1.348 −2.979 −3.481 DRD4-Tango −0.098 −1.477 0.834 1.274 −2.932 −0.672 −3.047 −2.867 DRD5-Tango −0.055 −0.128 −0.518 −0.713 −0.210 −1.014 −0.508 −0.646 EDNRA-Tango 0.254 −0.095 0.375 −0.278 −0.205 −0.148 −0.074 −0.045 EDNRB-Tango 0.108 0.126 0.081 0.501 −0.458 −0.590 −0.753 −0.605 ELTD1-Tango −0.447 −0.835 0.172 0.719 −0.694 −1.030 −0.723 −0.342 F2R-Tango 0.182 0.145 0.127 0.210 −0.075 −0.276 −0.070 −0.233 F2RL1-Tango 0.048 −0.052 −0.042 0.023 −0.163 −0.205 −0.221 −0.302 F2RL2-Tango −0.048 −0.385 0.061 0.219 −0.793 −0.304 −0.667 −0.643 F2RL3-Tango −0.255 −0.209 0.042 −0.298 −0.313 −0.283 −0.309 −0.318 FFA1-Tango 0.240 −0.080 0.193 0.319 −0.347 −0.520 −0.339 −0.009 FFA2-Tango −0.204 −0.127 0.375 0.188 −0.491 −0.987 −0.504 −0.732 FFA3-Tango 0.093 0.163 0.409 0.243 −0.371 −0.246 −0.618 −0.451 FPR1-Tango 0.494 0.535 0.975 0.956 0.185 −0.850 −1.458 −0.970 FPR2-Tango 0.101 0.263 0.745 0.732 −0.157 1.656 −1.231 −1.397 FPR3-Tango 0.566 −0.027 0.440 0.753 −0.431 −0.669 −0.626 −0.576 FSHR-Tango 0.075 −0.218 0.305 0.503 −0.705 −0.591 −0.548 −0.553 GABBR1-Tango 0.274 −0.218 0.281 0.424 −0.895 −0.711 −0.895 −1.337 GAL1-Tango −0.103 −0.229 0.455 1.284 −0.331 −2.280 −1.255 −0.856 GAL2-Tango 0.494 0.214 −0.347 0.171 −0.122 −1.208 −0.772 −0.585 GAL3-Tango −0.124 0.615 −0.086 −0.571 −0.585 −0.260 −1.122 −0.707 GCGR-Tango −0.020 0.284 −0.591 −0.316 −0.503 −0.709 −0.267 −0.878 GHRHR-Tango 0.424 0.605 0.017 0.209 −0.814 −0.665 −0.892 −0.637 GHSR-Tango 0.492 0.254 −0.801 −0.419 −0.394 −1.174 −0.848 −0.646 GIPR-Tango 0.280 0.323 −0.586 −0.547 −0.607 −0.997 −1.363 −0.809 GLP1R-Tango 0.234 −0.126 −0.819 −0.859 −0.685 −0.368 −1.357 −0.618 GLP2R-Tango 0.424 −0.068 −0.368 0.062 0.014 −0.605 −0.200 −0.014 GNRHR-Tango −0.026 −0.065 0.058 0.206 −0.537 −0.877 −0.485 −0.628 GPBA-Tango −0.993 −0.404 0.086 0.393 −1.048 −0.946 −0.900 −0.585 GPER-Tango 0.097 0.040 0.129 0.209 −0.284 −0.657 −0.569 −0.511 GPR1-Tango −0.249 −0.194 0.099 0.552 −0.917 −0.605 −1.179 −0.808 GPR101-Tango 0.607 0.080 −0.242 0.337 −0.817 −0.341 −0.792 −0.776 GPR110-Tango −0.141 −0.687 −0.449 −0.373 −0.798 −0.579 −0.765 −0.716 GPR111-Tango 0.012 −0.177 −0.125 −0.060 −1.273 −0.703 −0.968 −1.211 GPR113-Tango −0.387 −0.387 −0.345 −0.134 −0.256 −0.686 −0.209 −0.600 GPR114-Tango 0.155 −0.262 −0.154 −0.020 −0.326 −0.830 −0.479 −0.526 GPR115-Tango 0.039 −0.637 −0.106 0.118 −0.441 −0.720 −0.418 −0.358 GPR116-Tango −0.389 −0.334 0.125 0.263 −0.452 −1.116 −0.518 −0.407 GPR119-Tango −0.021 −0.387 0.200 −0.006 −0.492 −1.178 −0.599 −0.654 GPR12-Tango 0.120 −0.015 −0.380 −0.565 −0.680 −0.230 −0.396 −0.848 GPR120-Tango −0.204 −0.230 −0.066 0.505 −0.747 −0.548 −0.541 −0.622 GPR123-Tango 0.465 0.134 0.113 −0.238 −0.388 −0.611 −0.869 −0.742 GPR124-Tango −0.535 −0.461 0.337 0.200 −0.162 −0.716 −0.414 −0.358 GPR125-Tango −0.331 −0.798 0.377 0.815 −0.223 −0.741 −0.347 −0.420 GPR126-Tango −0.071 −0.088 0.167 0.258 −0.719 −0.801 −0.684 −0.902 GPR132-Tango −0.584 −0.354 −0.424 −0.550 −0.520 −0.539 −0.409 −0.689 GPR133-Tango −0.067 −0.359 −0.043 0.016 −0.403 −1.043 −0.349 −0.301 GPR135-Tango −0.045 0.307 −0.082 −0.307 −0.637 −0.848 −0.422 −0.637 GPR141-Tango 0.142 0.055 −0.165 −0.039 −0.488 −0.321 −0.538 −0.235 GPR142-Tango −0.831 −0.696 −0.205 −0.335 0.000 −0.736 −0.044 −0.095 GPR143-Tango −0.753 −0.709 −0.256 −0.650 −0.046 −0.120 −0.724 −0.380 GPR144-Tango −0.445 −0.642 −0.227 −0.348 −0.325 −0.745 −0.715 −1.340 GPR146-Tango −1.000 −0.047 −0.523 −0.263 −0.403 −0.723 −0.866 −0.335 GPR148-Tango −0.477 −0.658 1.350 1.263 −0.879 −0.087 −1.132 −0.511 GPR149-Tango −0.515 −0.551 0.107 0.795 −1.016 −1.030 −1.569 −1.374 GPR15-Tango −0.322 −0.260 −0.079 −0.795 −0.649 −1.096 −0.399 0.037 GPR150-Tango −0.376 −0.093 0.103 −0.082 −0.531 0.204 0.187 −0.267 GPR151-Tango −0.199 −0.600 0.336 0.178 0.058 −0.093 −0.559 0.000 GPR152-Tango −0.183 −0.224 0.189 0.785 0.090 −0.242 0.221 −0.075 GPR153-Tango 0.013 −0.295 0.114 0.337 −0.413 −0.473 −0.586 −0.670 GPR156-Tango 0.141 −0.170 0.837 1.681 −0.261 −0.913 −0.659 −0.611 GPR157-Tango −0.383 −0.047 0.370 0.610 −0.262 −0.370 −0.270 −0.413 GPR158-Tango −0.093 0.011 0.338 −0.020 0.121 −0.340 −0.155 −0.441 GPR160-Tango −0.173 −0.418 −0.095 −0.061 −0.050 0.074 −0.419 −0.318 GPR161-Tango −0.467 −0.743 −0.159 −0.208 −0.481 −0.445 −0.723 −0.518 GPR162-Tango −0.250 −0.391 −0.386 −0.361 −0.631 −0.852 −0.888 −0.671 GPR17-Tango −0.696 −0.878 0.942 0.350 0.073 −0.479 −0.036 0.013 GPR171-Tango −0.503 −0.672 0.286 0.120 −0.192 −0.799 −0.489 −0.224 GPR173-Tango −0.205 −0.365 −0.155 0.160 −0.302 −0.499 −0.275 −0.615 GPR174-Tango 0.045 −0.472 −0.180 −0.085 −0.197 −0.703 −0.175 −0.197 GPR18-Tango −0.220 −0.623 −0.110 −0.141 −0.017 −0.484 −0.997 −0.369 GPR182-Tango −0.471 −0.695 −0.121 0.232 −0.384 −0.594 −0.453 −0.369 GPR183-Tango −0.027 −0.211 −0.163 −0.025 −0.398 −0.516 −0.199 −0.316 GPR19-Tango −0.482 −0.608 −0.258 −0.055 −0.448 −0.890 −0.103 −0.023 GPR20-Tango 0.000 0.118 −0.037 −0.222 −0.585 −0.523 −0.322 −0.074 GPR21-Tango −0.078 −0.019 −0.056 0.236 −0.787 −0.511 −1.102 −0.601 GPR22-Tango 0.130 −0.624 −0.175 0.228 −0.406 −0.297 −0.602 −0.862 GPR25-Tango −0.426 0.016 0.087 −0.252 −0.429 −0.070 −0.284 −0.626 GPR26-Tango 0.483 0.137 0.048 −0.614 −0.034 −0.225 0.034 −0.118 GPR27-Tango −0.244 −0.227 0.205 0.057 −0.201 −0.081 −0.511 −0.609 GPR3-Tango 0.365 −0.124 0.203 −0.398 −0.301 0.220 −0.349 −0.393 GPR31-Tango −0.283 −0.132 0.051 −0.130 −0.412 −0.576 −0.402 −0.264 GPR32-Tango 0.040 0.097 −0.131 −0.237 −0.300 −0.794 −1.383 −0.300 GPR34-Tango −0.035 −0.614 0.146 0.092 −1.040 −0.508 −0.954 −0.954 GPR35-Tango −0.373 0.095 0.074 −0.169 −0.690 −0.248 −1.134 −0.767 GPR37-Tango 0.104 −0.215 0.260 0.364 −0.863 −0.525 −1.054 −1.014 GPR37L1-Tango 0.184 −0.198 0.262 0.432 −0.097 −0.622 −0.285 −0.548 GPR39-Tango −0.044 −0.062 −0.190 −0.270 −0.298 0.111 −0.586 −0.214 GPR4-Tango −0.041 −0.154 −0.173 −0.300 −0.861 −0.094 −0.872 −0.815 GPR44-Tango 0.279 0.313 −0.118 −0.214 −0.609 −0.371 −0.823 −0.823 GPR45-Tango 0.110 −0.097 −0.649 −0.321 −0.054 −0.951 −0.525 −0.525 GPR50-Tango 0.549 0.080 0.090 −0.222 −0.051 −0.181 −0.177 0.025 GPR52-Tango 0.461 0.125 −0.051 0.201 −0.817 0.062 −1.331 −0.469 GPR55-Tango 0.053 −0.055 0.495 0.141 −1.267 −0.461 −1.367 −0.688 GPR56-Tango −0.146 −0.049 −0.059 0.311 −0.944 −0.513 −0.763 −0.659 GPR6-Tango 0.117 −0.229 0.194 0.203 −0.443 −0.321 −0.761 −0.305 GPR61-Tango 0.156 0.005 0.018 0.072 −0.497 0.339 −0.805 −0.984 GPR62-Tango −0.023 0.125 0.042 0.194 −0.330 −0.914 −0.330 −0.477 GPR63-Tango −0.212 −0.513 0.083 0.306 −0.465 −0.901 −0.766 −0.622 GPR64-Tango −0.117 −0.404 0.039 0.346 −0.571 −1.001 −0.843 −0.862 GPR65-Tango 0.163 −0.116 0.150 0.284 −0.462 −0.522 −0.663 −0.815 GPR68-Tango −0.066 −0.202 −0.106 −0.037 −0.974 −0.868 −0.877 −1.182 GPR75-Tango 0.024 −0.028 −0.185 0.169 −0.377 −0.918 −0.797 −0.658 GPR77-Tango −0.223 −0.161 −0.185 −0.366 −0.977 −1.055 −0.985 −1.385 GPR78-Tango 0.485 −0.025 0.381 0.235 −0.226 −0.805 −0.334 −0.841 GPR82-Tango 0.010 −0.123 −0.263 0.103 −0.499 −0.884 −0.561 −0.898 GPR83-Tango −0.357 −0.252 −0.117 −0.151 −0.584 −0.335 −0.544 −0.400 GPR84-Tango −0.708 −0.606 −0.162 −0.065 −0.792 −0.485 −0.588 −0.703 GPR85-Tango −0.525 −0.438 0.064 0.322 −0.448 −0.730 −0.577 −0.577 GPR87-Tango −0.129 −0.280 0.206 0.430 −0.290 −0.467 −0.420 −0.354 GPR88-Tango −0.436 −0.203 0.096 0.350 −0.635 −0.691 −0.759 −0.635 GPR97-Tango −0.823 −0.550 −0.089 −0.101 −0.593 −1.005 −0.751 −0.607 GPRC5A-Tango −0.478 −0.311 0.183 0.756 −0.201 −0.976 −0.668 −0.457 GPRC5B-Tango −0.475 −0.742 0.122 0.405 −0.408 −0.262 −0.290 −0.450 GPRC5C-Tango −0.467 −0.728 −0.186 0.230 −0.143 −0.514 −0.098 −0.425 GPRC5D-Tango −0.238 −0.354 0.232 0.066 −0.641 −0.822 −0.647 −0.598 GPRC6A-Tango −0.278 −0.538 −0.037 0.216 −0.495 −0.505 −0.420 −0.388 GRM1-Tango −0.262 −0.461 −0.450 −0.362 −0.135 −0.687 −0.346 −0.234 GRM2-Tango −0.520 −0.439 −0.200 −0.015 −1.034 −0.793 −1.055 −0.903 GRM4-Tango −0.245 −0.343 −0.684 −0.379 −0.642 −0.086 −0.668 −0.577 GRM5-Tango −0.305 −0.511 −0.355 −0.153 −0.875 −0.596 −0.885 −1.070 GRM6-Tango −0.656 −0.263 −0.493 −0.115 −0.560 −0.408 −0.904 −0.620 GRM7-Tango −0.913 −0.792 −0.158 0.161 −0.409 −0.876 −0.553 −0.466 GRM8-Tango −0.803 −0.840 −0.021 0.170 −0.672 −0.748 −0.349 −0.349 GRPR-Tango −0.246 −0.246 −0.659 −0.945 −0.026 −0.575 −0.551 −0.354 HCA1-Tango −0.515 −0.280 −0.113 0.113 −0.138 −0.715 −0.138 0.082 HCA2-Tango −0.821 −0.724 0.665 0.369 −0.284 −0.149 −0.453 −0.534 HCA3-Tango −0.463 −0.360 0.046 0.388 −0.158 −0.605 0.000 −0.092 HCTR1-Tango 0.000 −0.511 0.032 0.285 −0.280 −0.649 0.935 −0.766 HCTR2-Tango 0.087 −0.093 0.260 0.358 −0.621 −0.563 0.632 −0.621 HRH1-Tango −0.867 −1.517 −0.004 0.270 −0.526 0.060 −0.456 −0.168 HRH2-Tango −0.677 −0.622 0.339 0.310 −0.390 −0.349 −0.108 0.081 HRH3-Tango −0.990 −0.810 0.309 0.473 −0.561 −0.189 0.648 0.972 HRH4-Tango −0.720 −0.550 0.643 1.060 −0.429 −0.510 0.465 0.813 HTR1A-Tango −0.313 −0.195 0.472 1.117 −0.642 −0.782 −0.610 −0.425 HTR1B-Tango −0.634 −0.562 0.072 0.574 −0.829 −0.292 −0.665 −0.701 HTR1D-Tango −0.290 −0.565 0.193 0.269 −0.551 −0.635 −0.668 −0.498 HTR1E-Tango −0.206 −0.215 0.231 0.525 −0.320 −0.088 −0.475 −0.063 HTR1F-Tango −0.655 −0.895 −0.287 0.316 −0.549 −0.963 −0.478 −0.623 HTR2A-Tango −1.239 −0.779 0.001 0.126 −0.747 −0.640 −0.869 −0.941 HTR2B-Tango −0.635 −0.947 0.336 0.825 −0.895 −0.325 −0.825 −0.870 HTR2C-Tango −0.967 −0.792 −0.030 0.126 −0.671 −0.817 −0.710 −0.632 HTR4-Tango −0.050 −0.020 −0.226 −0.219 −0.295 −0.568 −0.429 −0.842 HTR5-Tango −0.195 0.110 −0.318 −0.192 −0.139 −0.500 −0.311 −0.326 HTR6-Tango −1.071 −0.821 −0.096 0.111 −0.573 −0.454 −0.790 −0.848 HTR7-Tango −0.112 −0.947 0.244 0.623 −0.529 −0.860 −0.801 −0.944 KISSPEPTIN-R- −0.684 0.181 0.123 −0.142 0.335 −0.671 0.437 −0.700 Tango LHCGR-Tango −0.927 −1.227 −0.195 −0.069 0.381 −0.209 0.135 0.485 LPAR1-Tango −0.268 −0.273 0.252 0.330 −0.175 −0.156 −0.272 −0.485 LPAR2-Tango 0.000 −0.334 0.124 −0.156 −0.100 −0.497 −0.637 −0.637 LPAR4-Tango −0.998 −1.046 −0.081 −0.566 −0.071 −0.842 −0.778 −0.614 LPAR5-Tango −1.408 −0.925 −0.154 −0.514 −0.137 −0.745 −0.341 −0.395 LPAR6-Tango −0.817 −1.130 0.002 −0.304 −0.145 −0.220 −0.260 −0.365 LTB4R-Tango −0.667 −0.285 0.009 −0.303 0.287 −0.457 0.077 0.503 LTB4R2B-Tango −0.497 −0.671 0.109 0.037 0.204 0.059 −0.021 −0.289 MAS1-Tango −0.172 −0.267 −0.088 0.116 −0.421 −0.312 −0.609 −0.506 MAS1L-Tango −0.253 −0.763 0.461 0.715 −0.667 −0.378 −0.477 −0.621 MC1R-Tango −0.804 −0.662 0.204 0.163 −0.614 −0.574 −0.684 −0.778 MC2R-Tango 0.041 −0.255 0.216 −0.546 −0.409 −0.526 −0.520 −0.710 MC3R-Tango −0.075 −0.710 −0.140 0.064 −0.019 −0.126 −0.845 −0.131 MC4R-Tango −0.097 −0.064 −0.168 0.216 0.062 −0.530 −0.032 −0.628 MC5R-Tango −0.061 −0.061 0.208 0.607 0.240 −0.734 −0.473 −0.289 MCHR1-Tango −1.173 −1.386 −0.337 −0.288 −0.050 −0.744 −0.326 −0.270 MCHR2-Tango −0.605 −0.745 −0.167 −0.122 −0.719 −0.870 −0.810 −0.526 MLNR-Tango −0.436 −0.560 0.161 0.191 −0.933 −0.787 −0.559 −1.064 MRGPRD-Tango −1.261 −1.225 −0.303 −0.084 −0.329 −0.404 −0.529 −0.472 MRGPRE-Tango −0.926 −0.430 0.049 0.352 −0.439 −0.332 −0.588 −0.454 MRGPRF-Tango −0.549 −0.365 0.460 0.874 −0.299 −0.527 −0.360 −0.402 MRGPRG-Tango 0.400 −0.677 0.345 0.810 −0.607 −0.582 −0.473 −0.702 MRGPRX1-Tango −0.596 −0.823 −0.445 0.167 −0.183 −0.696 −0.124 −0.414 MRGPRX2-Tango −1.118 −1.068 −0.083 0.293 −0.752 −0.661 −1.248 −1.199 MRGPRX3-Tango −0.846 −0.426 −0.018 −0.071 −1.100 −0.774 −0.872 −0.964 MRGPRX4-Tango −1.283 −1.138 −0.395 −0.119 −1.258 −1.902 −1.424 −1.548 MTNR1A-Tango −0.168 −0.280 −0.572 −0.335 −0.729 −0.552 −1.318 −1.318 MTNR1B-Tango −0.845 −0.521 −0.245 0.072 −0.605 −0.923 −0.756 −0.940 NMBR-Tango −0.255 −0.144 0.192 0.294 −0.241 −1.158 −0.427 −0.444 NMUR1-Tango −0.341 0.317 −0.379 −0.475 0.222 −0.698 0.328 0.000 NMUR2-Tango −0.664 −0.060 −0.974 −1.268 −0.229 −0.187 −0.124 −0.387 NPBW1-Tango 0.281 0.065 0.435 1.030 −0.640 −1.328 −1.225 −2.506 NPBW2-Tango −0.482 −0.708 0.386 1.019 −1.746 −1.373 −1.323 −1.999 NPFF1-Tango −0.047 −0.288 0.110 0.794 −1.110 −1.227 −1.303 −1.360 NPFF2-Tango −0.096 −0.440 0.110 −0.229 0.027 −0.596 −0.065 −0.560 NPS-Tango −0.062 −0.351 −0.234 0.100 0.020 −0.793 −0.549 −0.225 NPY1R-Tango −0.491 −0.546 0.169 −0.108 −0.078 −1.012 −0.423 −1.078 NPY2R-Tango −0.195 −0.471 0.176 0.301 −1.671 −0.608 −1.871 −1.522 NPY4R-Tango 0.409 0.382 −0.267 −0.030 −0.798 −0.842 −0.991 −0.954 NPY5R-Tango −0.889 −0.968 −0.098 −0.199 −0.560 −0.445 −1.293 −1.382 NTSR1-Tango −0.597 −0.664 0.124 0.146 −0.897 −0.730 −1.551 −1.108 NTSR2-Tango −0.883 −1.037 0.132 −0.127 −0.284 −0.939 −0.748 −0.797 OPN3-Tango −0.045 −0.131 0.000 −0.129 −1.143 −0.371 −0.756 −1.022 OPN5-Tango −0.884 −0.889 −0.198 −0.046 −0.879 −0.321 −2.103 −1.672 OPRD1-Tango −1.061 −0.546 −0.144 −0.017 −0.280 −1.157 −0.995 −0.481 OPRK1-Tango 0.293 −0.732 0.419 0.719 −0.167 −0.136 −0.182 −0.110 OPRL1-Tango 0.034 0.034 0.348 0.096 −0.756 −0.565 −0.127 −0.422 OPRM1-Tango −0.190 −0.140 −0.383 −0.161 −0.640 −0.957 −0.493 −0.194 OXER1-Tango 0.000 −0.082 0.305 0.629 −0.419 −0.439 −0.384 0.057 OXGR1-Tango −0.931 −0.436 −0.331 −0.352 −0.316 −1.424 −0.883 −0.703 OXTR-Tango −0.086 −0.245 −0.007 0.101 −1.058 −0.862 −1.077 −0.758 P2RY1-Tango −1.003 −0.824 0.295 0.837 −1.547 −0.749 −1.663 −1.035 P2RY10-Tango −0.508 −0.349 −0.078 −0.113 −1.560 −0.546 −1.430 −0.515 P2RY11-Tango −0.559 −0.482 −0.049 −0.036 −0.617 −0.752 −0.814 −1.043 P2RY12-Tango −0.263 0.571 −0.387 0.372 −1.094 −0.822 −1.616 −1.906 P2RY13-Tango −0.268 −0.343 0.412 0.412 −0.509 −0.421 −0.538 −0.530 P2RY14-Tango −0.559 −0.551 0.606 0.466 −1.253 −0.380 −1.095 −0.905 P2RY2-Tango −0.660 −0.711 0.049 0.400 −1.159 −0.566 −1.342 −0.962 P2RY4-Tango −0.861 −0.567 −0.014 0.182 −1.269 −0.806 −1.405 −1.057 P2RY6-Tango −0.567 −0.771 0.646 0.868 −0.986 −0.425 −1.147 −0.786 P2RY8-Tango −0.148 −0.370 0.173 0.149 −0.635 −0.408 −0.496 −0.573 PK1-Tango −0.655 −0.766 0.072 0.078 −0.094 −0.954 −0.588 −0.430 PK2-Tango −0.222 0.071 0.187 0.176 −0.227 0.061 0.000 −0.176 PRRP-Tango −0.678 −0.575 0.051 0.088 −0.758 −0.970 −1.019 −1.307 PTAFR-Tango −0.729 −1.244 0.028 0.440 −0.737 −0.543 −1.194 −1.194 PTGDR-Tango −1.097 −0.538 −0.022 0.053 −0.961 −0.851 −1.517 −1.259 PTGER1-Tango −0.213 0.222 0.173 −0.104 −0.733 −1.208 −0.913 −0.529 PTGER2-Tango 0.126 0.399 0.183 0.034 −0.601 −0.429 −0.835 −0.923 PTGER3-Tango −0.173 −0.657 0.143 0.456 −0.192 −0.303 −0.551 −0.153 PTGER4-Tango 0.219 −0.408 0.202 0.489 −0.247 −0.088 −0.648 −0.162 PTGFR-Tango −0.458 −0.503 −0.462 −0.094 −0.098 −0.320 −0.601 −0.485 PTGIR-Tango −0.866 −0.987 0.045 0.045 −0.176 −0.722 −0.301 −1.024 PTH1R-Tango 0.020 −0.784 −0.404 −0.184 −0.447 −0.400 −0.709 −0.611 PTH2R-Tango −0.314 −0.825 −0.121 0.154 −0.647 −0.710 −0.804 −0.677 QRFP-Tango −0.224 −0.273 −0.515 −0.065 −0.154 −0.719 −0.280 −0.739 RXFP1-Tango −0.233 −0.754 −0.183 −0.019 −0.344 −0.693 −0.430 −0.170 RXFP2-Tango −0.009 −0.968 0.181 0.099 −0.825 −0.341 −0.257 −0.627 RXFP3-Tango 0.118 −0.357 −0.521 −0.218 −0.410 −0.632 −0.277 −0.258 RXFP4-Tango 0.471 −0.310 −0.611 −0.322 0.000 −0.714 −0.685 −0.403 S1PR1-Tango −0.322 −0.431 −0.313 −0.346 0.419 0.760 0.397 0.486 S1PR2-Tango −0.721 −0.736 −0.453 −0.869 0.357 −0.476 0.054 0.199 S1PR3-Tango −0.234 −0.379 −0.605 −0.653 0.598 0.130 0.295 0.648 S1PR4-Tango −1.385 −0.865 −0.470 −0.238 −0.152 −0.376 −0.089 0.400 S1PR5-Tango −0.346 −0.664 −0.114 0.523 0.229 −0.852 −0.220 −0.170 SCTR-Tango 0.488 −0.178 0.532 0.438 0.413 −0.808 −0.304 −0.640 SSTR1-Tango 0.127 −0.115 −0.442 −0.402 −0.143 0.000 −0.052 −0.655 SSTR2-Tango −0.596 −0.766 0.069 −0.211 0.830 −0.674 0.333 0.333 SSTR3-Tango −0.819 −0.953 0.000 −0.177 1.198 −1.678 0.667 0.542 SSTR4-Tango −0.873 −0.393 0.000 0.067 −0.505 −0.751 −0.156 0.295 SSTR5-Tango −0.705 0.506 1.503 0.931 −0.032 −0.798 0.062 −0.122 SUCNR1-Tango 2.561 −0.287 0.860 0.966 0.088 −1.806 2.206 −0.177 TA1-Tango −0.514 −0.849 0.375 −0.020 −0.549 −0.342 −0.617 −0.859 TAAR2-Tango −0.434 −0.708 0.174 −0.198 0.201 −0.534 −0.068 0.027 TAAR5-Tango −0.354 −0.849 0.201 0.753 −0.360 −1.336 −0.609 −0.807 TAAR6-Tango −0.312 −0.899 0.248 0.605 −0.670 −0.864 −0.466 −0.422 TAAR8-Tango −0.258 −0.471 0.333 0.836 −0.153 −0.551 −0.052 0.077 TAAR9-Tango −0.054 −0.338 0.138 0.088 −0.358 −0.207 −0.207 −0.333 TACR1-Tango −0.554 −0.706 0.206 0.225 −0.452 −0.861 −0.552 −0.740 TACR2-Tango −0.153 −0.391 0.268 −0.086 −0.282 0.013 −0.392 −0.403 TACR3-Tango −0.636 −0.943 0.070 0.507 −0.172 −0.847 −0.622 −0.428 TBXA2R-Tango −0.263 −0.173 −0.380 −0.411 −0.531 −0.700 −0.264 −0.700 TSHR-Tango −0.414 −0.812 −0.316 −0.056 −0.314 −0.871 −0.114 −0.375 UTS2R-Tango 0.000 0.050 −0.126 −0.269 0.369 −0.602 0.156 0.204 VIPR1-Tango −0.958 −0.958 0.197 0.404 −0.140 −0.218 −0.696 −0.834 VIPR2-Tango −1.615 −1.112 1.431 1.249 −0.106 1.436 −0.737 0.145 PRESTO-Tango Lactacillus Pediococcus Pediococcus Pediococcus Streptococcus Streptococcus Streptococcus Streptococcus with GMM UC UC-1# UC-2# UC-2# luteciae spp-1# spp-2# spp-3# ADCYAP1R1- −0.774 −0.992 −0.188 −0.717 −0.518 −0.313 −0.589 −0.050 Tango ADORA1-Tango −1.293 −0.951 −0.665 −0.933 −0.962 −0.615 −1.283 −0.531 ADORA2A-Tango −0.801 −1.163 0.173 −0.304 −0.528 −0.240 0.055 −0.413 ADORA2B-Tango −0.308 −0.699 −0.183 −0.270 −0.635 −0.249 −0.143 −0.470 ADORA3-Tango −0.211 −0.095 −0.063 −0.012 −0.202 0.167 0.039 0.103 ADRA1A-Tango −0.137 −0.510 −0.125 −0.117 −0.643 −0.483 −0.535 −0.426 ADRA1B-Tango −0.208 −0.445 −0.308 0.387 −0.208 0.041 −1.192 0.075 ADRA1D-Tango −0.608 −0.523 −0.407 −0.058 −0.261 −0.173 −0.374 −0.218 ADRA2A-Tango −1.000 −0.607 −1.542 −0.240 −1.733 −0.176 0.670 −0.647 ADRA2B-Tango −1.735 −1.179 −1.336 −0.929 −0.650 0.153 −0.491 −0.249 ADRA2C-Tango −0.913 −1.159 0.179 −0.038 −0.642 0.455 0.467 −0.288 ADRB1-Tango −0.153 −0.447 −0.285 −0.394 −0.517 −0.312 0.300 0.559 ADRB2-Tango −0.876 −0.974 −0.822 −0.248 −0.088 −0.040 −0.913 0.229 ADRB3-Tango −0.520 −1.127 −0.573 0.202 −0.535 −0.371 0.000 −0.087 AGTR1-Tango −0.651 −0.946 −0.159 0.128 −0.250 −0.256 −0.171 −0.256 AGTR2-Tango −0.509 −0.354 −0.645 −0.367 −0.203 0.012 −0.813 −0.137 APJ-Tango −1.079 −1.489 −0.843 −0.348 −0.418 −0.640 −0.758 −0.334 AVPR1A-Tango −0.510 −0.922 −0.284 0.090 −0.286 −0.286 −0.836 −0.087 AVPR1B-Tango −0.662 −1.017 −0.294 0.299 −0.385 −0.304 −0.706 0.000 AVPR2-Tango −0.987 −0.941 −0.836 −0.122 −0.778 −0.088 −1.338 −0.428 BB3-Tango −1.019 −1.109 −0.518 −0.690 −0.622 −0.359 −0.856 −0.421 BDKBR1-Tango −0.596 −0.893 −0.098 0.071 −0.454 −0.349 0.015 −0.468 BDKBR2-Tango −0.304 −0.160 −0.207 0.013 0.040 −0.120 −0.121 0.256 C3AR1-Tango −0.737 −0.821 −0.569 −0.279 −0.273 −0.442 −0.569 −0.295 C5A-Tango −0.526 −0.746 −0.501 0.191 −0.030 −0.216 −1.035 0.344 CALCRb-Tango −0.485 −0.946 −0.091 −0.320 −0.481 −0.280 −0.301 0.015 CALCRL-Tango −0.439 −0.760 0.252 0.175 −0.414 −0.131 0.020 −0.304 CASR-Tango −0.345 −0.767 0.039 0.269 −0.068 −0.167 −0.478 0.547 CCKAR-Tango −0.198 −0.202 0.028 0.129 −0.182 −0.080 −0.650 0.350 CCKBR-Tango −0.422 −0.607 −0.344 −0.059 −0.456 −0.472 −0.729 −0.147 CCR10-Tango −1.000 −1.648 −0.280 −0.251 −0.470 0.020 −0.818 −0.369 CCR2-Tango −0.059 −0.373 −0.225 −1.050 −0.206 −0.363 −0.097 −0.354 CCR3-Tango −0.442 −0.497 −0.641 −0.956 −0.585 −0.494 −0.710 −0.010 CCR4-Tango −0.680 −1.088 −0.252 −0.329 −0.329 0.022 0.025 0.492 CCR5-Tango −0.171 −0.551 −0.324 0.148 −0.231 −0.142 −0.161 0.691 CCR6-Tango −0.305 −0.752 −0.598 0.031 0.217 0.148 −0.455 0.847 CCR7-Tango −0.658 −1.310 0.117 −0.221 −0.081 −0.645 0.000 0.253 CCR8-Tango −0.700 −1.652 −1.097 −0.502 −0.522 −0.178 −0.688 −0.569 CCRL2-Tango −0.782 −1.802 0.212 0.383 −0.212 −0.014 0.334 0.258 CD97-Tango −0.329 −0.985 −0.193 0.160 −0.788 −0.235 0.740 −0.381 CHRM1-Tango 0.122 −0.046 −0.136 0.510 −0.558 −0.221 −1.100 −0.204 CHRM2-Tango 0.608 0.082 0.433 0.965 −0.567 −0.337 −0.993 0.042 CHRM3-Tango 1.038 −0.176 0.760 0.600 −0.945 −0.197 −0.266 0.213 CHRM4-Tango 1.248 −0.238 −0.244 0.096 −0.450 −0.136 −0.234 0.528 CHRM5-Tango 0.085 −0.500 0.095 0.037 −0.403 −0.314 −0.431 0.905 CMKLR1-Tango −0.180 −0.555 −0.217 −0.022 −0.445 −0.246 −0.605 0.255 CNR1-Tango −0.792 −1.064 −0.402 0.287 −0.258 −0.452 −0.682 0.060 CNR2-Tango −0.537 −0.852 −0.104 0.082 −0.282 −0.544 −0.369 −0.353 CRHR1-Tango −0.229 −0.904 −0.373 −0.202 −1.119 −0.434 0.170 −0.171 CRHR2-Tango −0.211 −0.702 −1.086 −0.341 −0.054 0.000 −0.844 0.300 CX3C1-Tango −0.328 −0.791 −0.335 −0.621 −0.720 −0.985 −0.358 −0.070 CXCR1-Tango −0.238 −1.257 −0.675 −0.033 −0.031 −0.101 0.165 0.288 CXCR2-Tango −0.809 −0.980 −0.589 −0.944 −1.148 0.142 −0.866 0.027 CXCR3-Tango −1.007 −1.098 −0.355 −0.148 −0.538 −0.144 −0.500 −0.190 CXCR4-Tango −0.610 −0.557 0.121 −0.084 −0.421 0.461 −0.543 0.073 CXCR5-Tango −0.309 −0.754 0.124 −0.096 −0.588 0.447 −0.039 −0.229 CXCR6-Tango −0.665 −1.193 −1.007 −0.200 −0.079 −0.340 −0.046 0.547 CXCR7-Tango −1.517 −1.731 −0.778 −0.739 −0.478 −0.352 −0.316 −0.540 CYSLTR1-Tango −0.527 −0.899 −1.050 −0.564 −0.342 −0.433 −1.078 −0.134 CYSLTR2-Tango −0.553 −1.213 −0.364 −0.253 −0.344 −0.188 0.000 0.284 DRD1-Tango −1.196 −1.518 −0.320 −0.417 −0.382 −0.582 0.308 0.295 DRD2-Tango −1.035 −1.605 −0.588 −0.759 −0.844 −0.436 3.107 0.730 DRD3-Tango −2.036 −1.685 −0.775 0.079 −0.842 −0.106 2.972 0.537 DRD4-Tango −2.449 −2.449 −0.463 −0.026 −0.969 −0.047 2.135 0.497 DRD5-Tango −0.576 −0.861 −0.144 −1.026 −0.940 −0.771 −0.604 −0.320 EDNRA-Tango −0.405 −0.382 −0.222 −0.050 −0.437 −0.440 −0.632 0.117 EDNRB-Tango −0.470 −1.004 0.157 0.130 −0.617 −0.556 −0.064 −0.154 ELTD1-Tango −1.040 −0.813 −0.132 −0.738 −0.741 −0.764 −0.484 −0.073 F2R-Tango −0.216 −0.281 −0.207 −0.044 −0.357 −0.728 −0.092 0.343 F2RL1-Tango −0.348 −0.494 −0.290 0.146 −0.179 −0.622 −0.405 0.675 F2RL2-Tango −0.693 −0.447 0.092 −0.046 −0.379 −0.094 −0.585 0.449 F2RL3-Tango −0.361 −0.484 −0.312 −0.025 −0.311 −0.667 −0.474 0.286 FFA1-Tango −0.655 −0.736 0.306 −0.078 −0.528 −0.530 −1.036 0.138 FFA2-Tango −0.848 −1.125 0.141 0.040 −0.443 −0.264 −0.188 0.016 FFA3-Tango −0.722 −0.843 −0.258 0.090 −0.099 −0.208 0.253 0.433 FPR1-Tango −0.213 −0.221 −0.449 0.385 0.323 −0.313 −0.567 −1.225 FPR2-Tango −0.698 −0.990 −0.123 0.359 −0.397 −0.339 0.261 0.225 FPR3-Tango −0.699 −0.676 −0.278 −0.075 −0.645 −0.503 −1.149 −0.027 FSHR-Tango −0.573 −0.731 −0.138 −0.271 −0.373 −0.448 −0.430 0.209 GABBR1-Tango −0.252 −0.897 0.090 0.094 −0.266 −0.225 −0.051 −0.091 GAL1-Tango −1.196 −1.940 −1.174 −0.448 −1.262 −1.112 −0.526 −0.564 GAL2-Tango −0.234 −1.390 −0.298 −0.027 0.629 −0.181 −0.554 0.369 GAL3-Tango −1.043 −1.165 −1.198 0.027 0.156 −0.771 −0.700 −0.501 GCGR-Tango −0.777 −0.955 −0.500 −0.361 −0.275 −0.528 −0.642 0.072 GHRHR-Tango −0.657 −1.230 −0.462 −0.410 −0.567 −0.476 −1.134 0.079 GHSR-Tango −0.922 −0.869 −0.215 −0.814 −0.300 −0.429 −1.503 0.006 GIPR-Tango −1.120 −1.191 −0.401 −0.803 −0.461 −1.021 −0.829 0.430 GLP1R-Tango −0.503 −0.303 −0.451 −0.281 0.068 −0.188 −0.995 0.185 GLP2R-Tango −0.520 −0.734 −0.782 −0.234 −0.193 0.147 −1.647 0.152 GNRHR-Tango −0.570 −1.570 0.112 0.089 −0.414 −0.226 −0.458 0.085 GPBA-Tango −0.615 −0.081 −0.549 0.235 −0.356 −0.071 −1.194 −0.449 GPER-Tango −0.435 −1.339 −0.365 −0.369 −0.862 −0.734 −0.171 0.335 GPR1-Tango −0.990 −1.334 −0.426 −0.556 −0.589 −0.397 −0.788 0.144 GPR101-Tango −0.603 −0.957 −0.534 0.023 −0.339 −0.429 −1.332 0.224 GPR110-Tango −0.389 −0.599 −0.274 −0.049 −0.275 −0.714 −0.600 0.256 GPR111-Tango −0.555 −1.020 −0.402 −0.185 −0.648 −0.689 −0.252 −0.059 GPR113-Tango −0.978 −0.748 −0.207 −0.631 −0.377 −0.213 −0.484 −0.135 GPR114-Tango −0.384 −0.627 −0.298 −0.290 −0.492 −0.227 −0.464 −0.071 GPR115-Tango −0.686 −0.737 −0.322 −0.381 −0.351 −0.003 −0.746 −0.204 GPR116-Tango −0.438 −0.772 −0.345 −0.377 −0.333 −0.255 −0.380 0.061 GPR119-Tango −0.650 −1.041 −0.236 0.056 −0.521 0.026 −0.116 −0.073 GPR12-Tango −0.269 −0.768 −0.518 −0.214 −0.303 −0.039 −0.512 0.278 GPR120-Tango −0.904 −0.556 0.247 −0.011 −0.721 −0.204 −0.387 −0.412 GPR123-Tango −0.286 −0.504 −0.485 0.145 −0.027 −0.134 −0.872 0.298 GPR124-Tango −0.708 −1.064 −0.259 0.093 −0.596 −0.101 −0.484 −0.005 GPR125-Tango −0.639 −0.999 −0.561 −0.082 −0.376 −0.053 −0.861 0.284 GPR126-Tango −0.927 −1.471 −0.166 −0.158 −0.490 −0.420 −0.537 −0.090 GPR132-Tango −0.847 −0.989 0.000 0.366 0.385 −0.201 −1.274 −0.268 GPR133-Tango −0.853 −1.139 −0.011 −0.003 0.097 −0.179 −0.803 −0.120 GPR135-Tango −0.816 −1.198 −0.440 −0.141 −0.018 −0.423 −0.694 −0.150 GPR141-Tango −0.989 −0.751 −0.023 0.054 −0.333 −0.269 −0.031 −0.640 GPR142-Tango −0.760 −1.088 −0.296 0.348 −0.092 −0.256 −1.040 −0.297 GPR143-Tango −1.044 −0.724 −0.299 0.103 −0.131 −0.334 −0.931 0.658 GPR144-Tango −0.423 −0.858 −0.110 −0.227 −0.843 −0.474 −0.515 −0.547 GPR146-Tango −0.317 −0.243 −0.066 −0.263 −0.688 −0.812 −0.404 0.263 GPR148-Tango −0.066 −0.066 −0.170 1.186 −0.483 0.216 −0.404 0.697 GPR149-Tango −1.135 −1.052 −0.033 0.244 −0.509 0.017 −1.033 0.541 GPR15-Tango −0.594 −0.955 −0.613 0.025 −0.493 −0.144 0.000 0.832 GPR150-Tango −0.525 −0.866 −0.160 0.245 −0.788 −0.800 −0.160 0.282 GPR151-Tango −0.294 −0.726 −0.009 0.701 −0.295 −0.196 −0.538 0.591 GPR152-Tango −0.485 −0.517 −0.281 0.170 −0.262 −0.293 −0.245 −0.442 GPR153-Tango −0.534 −0.595 −0.598 −0.021 −0.249 −0.023 −0.788 0.126 GPR156-Tango −0.662 −0.752 −0.858 −0.065 −0.568 0.086 −0.764 −0.513 GPR157-Tango −0.327 −0.937 −0.101 0.253 −0.271 −0.213 −0.215 0.407 GPR158-Tango −0.114 −0.405 −0.432 0.431 0.029 −0.128 −0.399 −0.051 GPR160-Tango −0.375 −0.777 −0.310 0.557 0.466 −0.056 −0.429 0.458 GPR161-Tango −0.747 −0.899 −0.162 0.189 0.009 −0.207 −0.391 0.704 GPR162-Tango −0.903 −1.255 −0.266 −0.240 −0.386 −0.544 −0.442 −0.042 GPR17-Tango −1.140 −0.881 −0.120 0.062 −0.759 0.075 −0.565 0.378 GPR171-Tango −1.126 −1.220 0.058 0.424 −0.314 0.255 −0.146 0.069 GPR173-Tango −0.943 −1.049 −0.366 0.625 −0.436 −0.422 −0.386 0.084 GPR174-Tango −0.575 −1.012 0.668 0.448 0.108 −0.071 0.351 −0.300 GPR18-Tango −0.820 −1.251 0.062 0.304 0.078 −0.341 0.379 0.270 GPR182-Tango −1.006 −1.354 −0.099 0.254 −0.293 −0.204 −0.730 0.318 GPR183-Tango −0.730 −1.323 0.035 0.339 −0.424 −0.276 −0.006 0.020 GPR19-Tango −0.817 −0.612 0.054 0.476 −0.430 −0.124 −0.208 0.601 GPR20-Tango −0.241 −1.082 1.372 0.193 −0.205 0.000 0.497 0.511 GPR21-Tango 0.064 −1.000 0.055 0.135 −0.117 0.016 0.200 0.387 GPR22-Tango −0.485 −0.672 −0.318 0.280 −0.007 −0.035 −0.033 0.556 GPR25-Tango −0.362 −0.988 0.144 0.023 −0.389 −0.060 0.275 −0.129 GPR26-Tango −0.690 −0.769 −0.164 0.083 −0.390 −0.420 −0.243 0.720 GPR27-Tango 0.126 −0.347 0.053 −0.109 −0.628 0.039 0.111 0.047 GPR3-Tango −1.107 −1.304 0.022 0.124 −0.669 −0.467 −0.278 −0.345 GPR31-Tango −0.931 −1.429 0.354 0.041 −0.542 −0.365 0.331 −0.012 GPR32-Tango −0.648 −0.911 −0.371 −0.510 −0.866 −0.232 −1.299 −0.180 GPR34-Tango −0.820 −1.659 −0.189 −0.426 −0.925 −0.333 −0.356 −0.264 GPR35-Tango −1.019 −1.516 −0.449 −0.098 −0.433 −0.140 −1.073 −0.290 GPR37-Tango −0.255 −0.835 −0.148 −0.258 −0.631 0.066 −1.033 0.022 GPR37L1-Tango −0.359 −0.763 −0.384 −0.264 −0.378 0.248 −0.886 0.401 GPR39-Tango −0.540 −0.902 −0.421 −0.399 −0.577 −0.285 −0.773 −0.321 GPR4-Tango −0.884 −0.759 0.202 −0.224 −0.321 −0.019 −1.165 0.056 GPR44-Tango −0.968 −1.126 −0.110 −0.439 −0.404 −0.511 −0.714 −0.205 GPR45-Tango −0.444 −0.409 −0.038 −1.284 −0.518 −0.399 −0.273 0.374 GPR50-Tango 0.125 −0.685 −0.050 0.402 −0.301 −0.064 −0.120 0.798 GPR52-Tango −0.974 −0.849 −0.436 −0.236 −0.308 −0.189 −0.497 0.611 GPR55-Tango −0.371 −0.437 −0.202 −0.049 −0.436 −0.098 0.392 0.540 GPR56-Tango −0.579 −0.606 0.574 −0.061 −0.596 −0.010 0.905 0.507 GPR6-Tango −0.386 −0.712 0.122 −0.197 −0.145 −0.033 −0.252 0.071 GPR61-Tango −0.375 −0.285 0.008 −0.138 −0.078 0.000 −0.557 0.380 GPR62-Tango −0.324 −0.423 0.353 0.172 −0.415 −0.180 0.170 0.473 GPR63-Tango −0.862 −0.696 −0.644 −0.141 −0.157 −0.071 −0.740 −0.290 GPR64-Tango −0.640 −0.798 −0.625 −0.382 −0.230 −0.259 −0.907 −0.102 GPR65-Tango −0.370 −0.296 −0.401 −0.138 −0.180 −0.135 −0.814 0.087 GPR68-Tango −0.350 −0.356 −0.183 −0.291 −0.481 −0.326 −1.241 −0.253 GPR75-Tango −0.090 −0.406 −0.280 −0.378 −0.308 −0.185 −0.420 0.446 GPR77-Tango −0.656 −0.830 −0.745 −0.896 −0.725 −0.169 −0.927 −0.101 GPR78-Tango −0.357 −0.506 −0.082 0.186 −0.185 0.022 −0.037 0.377 GPR82-Tango −0.784 −0.826 −0.672 −0.638 −0.452 −0.104 −0.689 −0.039 GPR83-Tango −0.133 −0.361 −0.344 −0.172 −0.277 −0.013 −0.570 0.037 GPR84-Tango −0.087 0.085 −0.308 −0.480 −0.362 0.005 −0.297 0.132 GPR85-Tango −0.116 −0.563 0.030 −0.173 −0.329 0.135 −0.121 −0.246 GPR87-Tango −0.207 −0.237 −0.417 −0.307 −0.340 0.191 −0.655 −0.088 GPR88-Tango −0.107 −0.631 −0.192 0.000 −0.550 −0.071 −0.316 −0.081 GPR97-Tango −0.390 −0.674 −0.139 −0.234 −0.242 −0.026 −0.597 0.000 GPRC5A-Tango −0.335 −0.149 −0.041 −0.485 −0.182 −0.043 −0.175 0.125 GPRC5B-Tango −0.260 −0.330 −0.181 −0.245 −0.131 −0.035 0.200 0.435 GPRC5C-Tango 0.114 0.000 −0.400 −0.289 0.088 −0.095 −0.144 0.482 GPRC5D-Tango −0.309 −0.399 −0.476 −0.122 −0.314 0.027 −0.946 −0.209 GPRC6A-Tango 0.031 −0.081 −0.428 −0.306 −0.428 0.428 −0.575 −0.498 GRM1-Tango −0.198 −0.173 −0.198 −0.508 −0.336 0.283 −0.690 0.358 GRM2-Tango −0.324 −0.547 −0.369 −0.343 −0.403 −0.139 −0.228 −0.035 GRM4-Tango 0.007 0.153 −0.048 −0.152 0.538 −0.087 −0.284 0.665 GRM5-Tango −0.080 −0.416 −0.618 0.306 0.251 −0.236 −1.275 0.585 GRM6-Tango −0.043 −0.383 −0.997 −0.493 −0.704 −0.050 −0.891 −0.039 GRM7-Tango −0.199 −0.175 −0.757 −0.652 −0.557 −0.553 −1.115 −0.685 GRM8-Tango −0.394 −0.691 −0.216 −0.028 −0.495 −0.411 −0.082 0.372 GRPR-Tango 0.148 −0.231 −0.318 −0.095 0.141 −0.270 −0.134 0.545 HCA1-Tango −0.250 −0.610 0.118 −0.195 0.158 0.094 0.258 0.112 HCA2-Tango 0.110 0.199 0.108 0.036 −0.095 −0.077 −0.448 0.565 HCA3-Tango −0.481 −1.216 0.356 0.072 −0.200 −0.218 0.215 −0.402 HCTR1-Tango −0.664 −0.781 0.313 −0.089 −0.508 0.000 0.801 0.377 HCTR2-Tango −0.719 −0.719 −1.132 0.480 −0.382 −0.234 −0.341 0.376 HRH1-Tango −0.480 −0.604 −0.434 −0.076 −0.808 −0.271 −0.952 0.235 HRH2-Tango 0.703 0.075 0.212 0.233 −0.601 0.128 0.057 −0.056 HRH3-Tango −0.572 −0.011 −0.913 0.197 −0.605 0.160 −0.781 0.056 HRH4-Tango −0.940 −0.898 −0.462 0.184 −0.560 −0.241 0.017 0.325 HTR1A-Tango −0.454 −0.676 −0.029 0.198 −0.562 0.198 1.566 0.124 HTR1B-Tango −0.673 −0.687 −0.373 −0.132 −0.746 −0.306 −0.136 0.196 HTR1D-Tango −0.797 −1.029 −0.412 −0.043 −0.473 −0.416 0.176 −0.168 HTR1E-Tango −0.741 −0.785 −0.107 −0.088 −0.476 −0.062 −0.381 0.259 HTR1F-Tango −0.393 −0.502 0.199 −0.365 0.139 −0.287 0.131 −0.009 HTR2A-Tango −0.547 −0.788 −0.089 −0.141 −0.285 −0.605 −0.634 0.072 HTR2B-Tango −1.637 −0.419 0.192 −0.162 −0.308 −0.165 −0.748 0.111 HTR2C-Tango −1.285 −1.120 −0.087 −0.066 −0.555 −0.389 −0.156 0.052 HTR4-Tango −1.990 −1.780 −0.056 −0.703 −0.327 −0.589 0.829 −0.055 HTR5-Tango −1.263 −1.224 −0.095 −0.495 0.621 −0.504 −0.076 0.246 HTR6-Tango −1.238 −1.703 0.120 0.215 0.206 −0.545 0.826 0.093 HTR7-Tango −2.771 −2.662 0.124 0.281 1.407 −0.615 −0.139 0.744 KISSPEPTIN-R- −0.022 0.097 −0.184 0.123 −0.804 −0.492 −0.184 0.054 Tango LHCGR-Tango −0.111 0.199 −0.015 −0.016 −0.294 −0.285 −0.130 0.228 LPAR1-Tango −0.107 −0.186 −0.285 0.039 −0.312 −0.368 −0.111 0.141 LPAR2-Tango −0.370 −0.933 −0.411 −0.053 −0.284 −0.431 −0.010 −0.119 LPAR4-Tango −0.338 −0.202 −0.470 −0.017 −0.352 −0.636 −0.796 0.096 LPAR5-Tango −0.443 −0.770 −0.079 −0.136 −0.301 −0.409 −0.390 0.166 LPAR6-Tango 0.155 0.123 −0.136 0.349 −0.100 −0.420 0.288 0.636 LTB4R-Tango −0.202 −0.202 −0.014 −0.032 −0.440 −0.453 −0.042 0.128 LTB4R2B-Tango −0.259 −0.066 −0.046 −0.023 −0.257 −0.393 0.040 0.417 MAS1-Tango −0.327 −0.615 −0.265 −0.208 −0.778 −0.102 −0.678 −0.065 MAS1L-Tango −0.158 0.067 −0.382 −0.168 −0.338 −0.242 −0.933 0.189 MC1R-Tango −0.202 −0.517 −0.055 0.013 −0.569 −0.099 −0.527 0.173 MC2R-Tango −0.449 −0.635 −0.128 −0.166 −0.675 −0.166 −0.050 0.533 MC3R-Tango −0.530 −0.761 −0.858 0.161 0.088 −0.517 0.266 1.051 MC4R-Tango −0.299 −0.909 0.062 −0.558 −0.679 0.243 0.542 0.429 MC5R-Tango −0.344 −0.510 −0.365 −0.178 −0.498 −0.528 −0.187 0.289 MCHR1-Tango −0.420 −0.670 −1.177 −0.486 −0.206 0.158 −1.419 0.633 MCHR2-Tango −0.064 −0.305 −0.309 0.032 −0.104 −0.055 −0.501 0.469 MLNR-Tango −0.246 −0.952 −0.090 0.097 −0.511 0.242 −0.661 −0.011 MRGPRD-Tango −0.498 −0.955 −0.316 −0.333 −0.462 0.102 −0.715 −0.088 MRGPRE-Tango −0.830 −0.933 −0.307 −0.193 −0.384 0.099 −0.396 −0.064 MRGPRF-Tango −0.086 −0.311 −0.402 −0.488 −0.181 0.035 −0.192 −0.094 MRGPRG-Tango −0.867 −0.756 −1.096 −0.441 0.320 0.116 −1.058 0.095 MRGPRX1-Tango −1.459 −1.340 −0.919 −0.440 0.142 0.050 −0.626 0.870 MRGPRX2-Tango −1.029 −0.888 −0.759 −0.540 −0.678 0.118 −0.366 0.311 MRGPRX3-Tango −0.822 −1.276 0.181 −0.494 −0.820 −0.038 −0.357 −0.019 MRGPRX4-Tango −1.331 −1.196 −0.787 −1.045 −0.533 0.191 −1.239 −0.332 MTNR1A-Tango −0.555 −0.581 −1.342 −0.840 −0.865 0.446 −0.547 0.044 MTNR1B-Tango −0.377 −1.122 −1.266 −0.796 −0.792 0.107 0.112 0.236 NMBR-Tango −0.248 −0.451 −1.113 −0.202 0.065 0.060 −0.596 0.600 NMUR1-Tango −0.526 −0.876 −0.117 −0.627 −0.786 −0.214 0.193 −0.482 NMUR2-Tango −0.045 −0.877 0.122 −0.214 −0.013 0.237 0.807 −0.160 NPBW1-Tango −0.848 −1.350 0.533 0.234 −0.153 0.177 0.240 0.260 NPBW2-Tango −1.135 −1.069 −0.321 −0.511 −0.404 −0.011 −0.198 −0.302 NPFF1-Tango −0.387 −0.695 −1.191 −0.108 −0.340 0.336 −1.693 −0.228 NPFF2-Tango 0.072 −0.473 −0.341 0.404 0.459 0.205 −0.177 0.082 NPS-Tango −1.020 −1.278 −1.123 −0.105 −0.045 −0.307 −0.478 0.638 NPY1R-Tango −0.920 −1.039 −0.757 0.336 0.155 −0.072 −0.807 0.544 NPY2R-Tango −0.986 −0.390 −0.300 0.197 0.337 −0.020 −0.300 0.430 NPY4R-Tango −1.051 −1.316 −0.034 −0.214 −0.177 −0.250 −0.080 0.049 NPY5R-Tango −0.309 −0.896 −0.404 −0.288 −0.334 0.275 −0.318 −0.162 NTSR1-Tango −0.419 −0.593 −0.122 −0.282 −0.517 −0.295 −0.861 −0.829 NTSR2-Tango 0.152 −0.713 0.348 0.136 −0.478 −0.438 0.013 −0.561 OPN3-Tango −0.131 −0.758 −0.243 −0.421 −0.614 −0.044 −0.243 0.212 OPN5-Tango −0.650 −0.851 −0.378 −0.657 −0.659 −0.084 −0.560 −0.177 OPRD1-Tango −0.757 −1.503 −0.511 −0.350 −0.627 −0.189 −0.856 −0.374 OPRK1-Tango 0.176 −0.232 −0.213 0.289 −0.151 −0.276 −0.342 0.227 OPRL1-Tango −0.188 −0.834 −0.638 0.357 −0.304 −0.579 −0.873 0.317 OPRM1-Tango 0.516 −0.058 −0.231 −0.150 −0.399 −0.473 −0.588 0.016 OXER1-Tango 0.428 0.166 −0.429 −0.005 0.015 −0.117 −0.743 0.193 OXGR1-Tango −0.882 −1.212 −1.089 −0.502 −0.772 −0.170 −1.340 −0.480 OXTR-Tango −0.078 −0.952 0.131 −0.176 −0.677 −0.361 0.131 −0.094 P2RY1-Tango −0.458 −0.277 0.104 0.635 −0.198 −0.118 −0.705 −1.073 P2RY10-Tango −0.186 −0.650 −0.085 0.124 −0.236 −0.174 −0.176 −0.758 P2RY11-Tango −0.628 −0.655 0.335 −0.238 −0.343 −0.580 −0.153 −0.621 P2RY12-Tango −0.733 −0.552 −0.666 −0.547 −0.299 −0.269 0.413 −1.411 P2RY13-Tango −0.852 −0.825 −0.319 −0.089 −0.996 −0.481 −0.848 −0.287 P2RY14-Tango −1.112 −0.897 −0.384 −0.250 −0.826 −0.205 −0.597 −0.184 P2RY2-Tango −0.680 −0.998 −0.060 0.101 −0.813 −0.061 −0.463 −0.529 P2RY4-Tango −1.406 −1.603 −0.165 −0.220 −0.473 −0.117 −0.802 −0.511 P2RY6-Tango −1.316 −1.268 −0.301 −0.148 −0.994 0.122 −0.844 −0.360 P2RY8-Tango −1.154 −1.416 −0.617 −0.259 −0.296 −0.039 −0.770 −0.228 PK1-Tango −0.882 −1.564 −0.462 −0.096 −0.712 −0.337 −0.482 0.087 PK2-Tango −0.128 −0.619 −0.100 0.021 −0.582 0.041 −0.144 0.095 PRRP-Tango −0.794 −1.168 0.014 −0.076 −0.849 −0.398 −0.154 −0.217 PTAFR-Tango −0.714 −0.764 0.072 −0.057 −0.396 −0.177 −0.660 −0.549 PTGDR-Tango −0.484 −0.978 0.301 −0.411 −0.376 −0.325 −0.139 −0.260 PTGER1-Tango −0.234 −0.752 0.327 0.094 −0.457 −0.230 −0.036 0.322 PTGER2-Tango −0.156 −1.034 0.494 −0.146 −0.405 −0.218 0.292 −0.108 PTGER3-Tango −0.893 −0.736 −0.064 −0.142 −0.360 0.119 0.058 0.359 PTGER4-Tango −0.863 −0.921 −0.063 0.198 0.185 0.062 0.646 1.125 PTGFR-Tango −0.447 −1.136 0.098 −0.147 −0.532 −0.693 0.305 0.646 PTGIR-Tango −0.627 −1.300 0.528 −0.113 −0.495 −0.231 0.143 0.048 PTH1R-Tango −0.423 −1.045 −0.265 −0.038 −0.489 0.088 −0.265 0.141 PTH2R-Tango −0.893 −1.267 −0.380 −0.320 −0.190 0.126 −0.562 −0.134 QRFP-Tango −0.328 −0.700 0.118 −0.364 −0.378 −0.179 −0.154 0.013 RXFP1-Tango −0.690 −1.002 −0.168 −0.341 −0.071 −0.842 −0.264 0.000 RXFP2-Tango −0.712 −0.573 −0.217 −0.171 −0.291 −0.186 −0.406 0.037 RXFP3-Tango −0.498 −0.890 −0.293 −0.773 −0.342 −0.278 −0.699 −0.105 RXFP4-Tango −0.845 −0.790 0.082 −0.795 −0.370 −0.555 −0.213 −0.209 S1PR1-Tango −0.437 −1.345 −0.173 −0.675 −0.268 −0.137 0.081 −0.499 S1PR2-Tango −0.521 −1.161 −0.515 −0.482 −0.378 −0.390 0.055 −0.387 S1PR3-Tango 0.112 −0.139 −0.027 −0.234 0.038 −0.161 0.154 −0.138 S1PR4-Tango −0.945 −1.105 −0.628 −1.169 −0.854 −0.054 −0.984 −0.464 S1PR5-Tango −0.825 −1.240 0.198 −0.137 −0.624 −0.353 −0.087 −0.493 SCTR-Tango −0.611 −0.994 −0.342 −0.390 −0.759 −0.384 0.812 0.109 SSTR1-Tango −0.115 −0.503 −1.328 −0.195 −0.156 0.074 −0.371 0.558 SSTR2-Tango −0.071 −0.184 −0.761 −0.114 −0.230 −0.441 0.316 0.528 SSTR3-Tango −0.463 −0.563 0.480 0.025 −0.092 0.076 0.117 0.411 SSTR4-Tango −0.101 −1.787 −0.449 −0.397 −0.490 −0.224 −0.303 0.665 SSTR5-Tango −0.995 −1.488 −0.040 1.195 −0.019 0.862 0.293 1.284 SUCNR1-Tango −1.625 −1.803 −0.404 0.530 0.569 0.329 −0.153 0.685 TA1-Tango −0.494 −1.006 −0.198 −0.058 −0.204 0.272 0.043 0.401 TAAR2-Tango −0.296 −0.796 −0.366 −0.141 −0.212 −0.240 −0.419 −0.129 TAAR5-Tango −0.584 −1.044 −0.948 −0.342 −0.719 −0.073 −1.123 −0.443 TAAR6-Tango −0.531 −0.430 −0.639 −0.555 −0.327 −0.037 −0.749 −0.074 TAAR8-Tango −0.086 −0.276 −1.050 −0.315 −0.048 −0.022 −1.005 0.152 TAAR9-Tango −0.276 −0.475 −1.039 −0.091 −0.014 −0.425 −0.588 −0.124 TACR1-Tango −0.489 −0.511 −0.584 −0.364 −0.337 −0.309 −0.304 −0.037 TACR2-Tango −0.433 −1.278 −0.886 −0.385 −0.594 −0.393 0.132 0.077 TACR3-Tango −0.707 −0.865 −0.453 −0.536 −0.486 −0.061 0.186 0.078 TBXA2R-Tango −0.535 −1.284 −0.222 −0.411 −0.279 −0.203 −0.153 −0.107 TSHR-Tango −0.694 −0.451 −0.491 −0.103 −0.149 −0.075 −0.611 0.401 UTS2R-Tango −0.298 −0.600 −0.500 0.000 −0.630 −0.124 −0.680 0.260 VIPR1-Tango −0.110 −0.117 −1.633 −0.757 −0.308 −0.297 −1.072 0.027 VIPR2-Tango 0.837 0.104 −0.288 1.011 −0.280 0.140 0.533 1.405 Acidamino- Acidamino- Acidamino- Pepto- Pepto- PRESTO-Tango coccus coccus coccus Megasphaera Megasphaera niphilus niphilus Bacteroids with GMM spp-1# spp-2# spp-3# spp-1# spp-2# spp-1# spp-2# fragilis-1# ADCYAP1R1- −0.633 −0.401 −0.043 −0.319 −0.731 −0.248 −0.502 −0.828 Tango ADORA1-Tango −0.806 −0.979 −0.462 −1.083 −0.911 −0.490 −0.707 −1.707 ADORA2A-Tango −0.083 −0.636 −0.298 −0.306 −0.169 0.135 −0.379 −1.065 ADORA2B-Tango −0.060 −0.446 −0.174 −0.071 −0.201 −0.141 −0.792 −0.758 ADORA3-Tango 0.259 −2.307 0.514 0.556 0.446 −0.092 −0.292 −0.625 ADRA1A-Tango −0.333 0.101 −0.167 −0.192 −0.009 −0.174 −0.215 −0.934 ADRA1B-Tango −0.063 −0.257 0.070 −0.563 0.351 −0.754 −0.703 −0.713 ADRA1D-Tango −0.140 0.033 −0.028 −0.719 0.346 −0.292 −0.134 −0.899 ADRA2A-Tango −0.086 −1.567 −0.086 1.157 0.047 0.405 −0.085 −3.042 ADRA2B-Tango −0.812 −0.678 −0.671 −0.194 −0.715 −0.125 −0.089 −2.048 ADRA2C-Tango 0.108 −0.403 0.601 −0.047 −0.103 0.160 0.082 −1.370 ADRB1-Tango −0.226 −0.160 0.038 0.680 0.130 0.174 −0.089 −1.468 ADRB2-Tango −0.239 0.000 −0.159 0.143 −0.071 −0.199 −0.282 −1.262 ADRB3-Tango −0.110 −1.600 0.322 0.000 0.359 −0.154 −0.350 −0.926 AGTR1-Tango 0.043 −0.386 0.266 0.030 0.270 0.453 0.837 −1.188 AGTR2-Tango −0.246 0.202 −0.194 0.038 −0.002 −0.012 0.341 −0.923 APJ-Tango −0.363 0.201 0.100 −0.055 0.137 0.179 −0.339 −1.722 AVPR1A-Tango −0.188 −0.982 −0.233 −0.025 0.151 −0.255 0.000 −1.511 AVPR1B-Tango 0.266 −0.536 0.200 −0.069 0.214 −0.391 −0.311 −1.197 AVPR2-Tango −0.393 −0.956 −0.052 −0.088 −0.182 0.086 −0.086 −1.798 BB3-Tango −0.374 −0.791 −0.152 −0.407 −0.304 −0.286 −0.283 −1.421 BDKBR1-Tango −0.128 −0.978 0.135 −0.176 0.217 −0.020 0.107 −0.788 BDKBR2-Tango 0.136 0.135 0.186 0.450 0.211 −0.241 −0.144 −0.753 C3AR1-Tango −0.318 −0.505 −0.275 −0.276 −0.385 −0.027 0.118 −0.852 C5A-Tango 0.421 0.238 0.452 0.201 0.708 −0.639 −0.575 −0.534 CALCRb-Tango 0.000 −0.534 0.019 0.168 −0.035 −0.474 −1.007 −0.703 CALCRL-Tango 0.008 −0.428 −0.173 0.040 0.076 −0.371 −0.405 −0.508 CASR-Tango −0.098 −0.639 0.175 −0.501 −0.075 −0.691 −0.809 −0.685 CCKAR-Tango −0.060 −0.471 0.306 −0.394 0.076 −0.509 −0.512 −0.549 CCKBR-Tango −0.176 −0.768 −0.163 −0.603 −0.359 −1.005 −0.931 −0.972 CCR10-Tango −0.412 −0.829 −0.363 −0.459 −0.176 −0.612 −0.806 −1.185 CCR2-Tango −0.946 −0.293 −0.429 0.214 −0.044 −0.629 −0.928 −0.918 CCR3-Tango −1.078 0.096 −0.391 −0.449 −0.537 0.183 0.385 −1.934 CCR4-Tango −0.018 0.242 −0.040 0.216 −0.014 −0.690 −0.040 −1.028 CCR5-Tango 0.574 0.102 0.401 0.204 0.605 −0.170 −0.243 −0.746 CCR6-Tango 0.466 0.906 0.501 0.647 0.860 0.108 0.209 −0.240 CCR7-Tango −0.276 −0.595 −0.210 0.029 −0.017 −0.520 −0.565 −0.903 CCR8-Tango −0.445 −0.429 −0.085 0.223 0.399 0.405 −0.129 −1.238 CCRL2-Tango 0.640 −0.257 0.447 0.674 0.640 0.044 0.672 −1.017 CD97-Tango −0.218 −0.342 −0.020 0.049 0.284 −0.483 −0.756 −0.986 CHRM1-Tango 0.168 −1.445 0.102 −0.075 0.348 0.136 0.026 −0.566 CHRM2-Tango −0.197 −0.894 0.052 −0.204 −0.192 −0.410 −0.643 −0.936 CHRM3-Tango −0.443 −0.598 −0.241 0.233 −0.147 0.181 −0.034 −1.139 CHRM4-Tango −0.150 −0.969 −0.047 0.081 −0.036 0.490 0.331 −0.339 CHRM5-Tango 0.031 0.116 0.217 0.141 −0.112 −0.594 −0.803 −0.687 CMKLR1-Tango −0.091 −0.205 −0.130 0.013 0.117 −0.803 −0.296 −0.769 CNR1-Tango 0.591 −0.217 0.609 −0.016 0.609 −0.458 −0.343 −0.830 CNR2-Tango 0.082 −0.178 0.211 −0.058 0.160 −0.029 0.290 −0.781 CRHR1-Tango 0.226 0.310 −0.029 0.155 0.372 −0.160 −0.623 −1.622 CRHR2-Tango −0.445 −0.515 −0.311 0.378 0.077 −0.532 −0.197 −0.348 CX3C1-Tango −0.194 0.057 −0.471 −0.451 −0.639 −0.445 −0.105 −0.926 CXCR1-Tango 0.517 −0.308 0.619 −0.110 0.337 0.654 0.377 −1.007 CXCR2-Tango 1.466 0.331 1.330 −0.117 −0.463 −0.118 −0.114 −1.540 CXCR3-Tango 0.215 −0.384 0.340 −0.084 −0.063 0.362 0.079 −1.257 CXCR4-Tango 0.108 −0.123 0.343 0.393 −0.167 −0.205 −0.224 −0.895 CXCR5-Tango −0.050 −0.661 −0.014 0.260 0.035 −0.226 −0.010 −1.013 CXCR6-Tango 0.028 −0.564 −0.067 −0.050 −0.028 0.624 0.970 −1.442 CXCR7-Tango −0.043 −0.334 −0.027 −0.555 −0.430 0.173 0.232 −1.875 CYSLTR1-Tango 0.790 −0.314 0.905 −0.494 −0.555 0.081 −0.128 −1.761 CYSLTR2-Tango 0.054 −0.715 0.184 0.000 −0.133 0.500 0.340 −1.013 DRD1-Tango −0.279 −0.825 −0.204 −0.334 −0.391 0.928 0.727 −3.851 DRD2-Tango −0.597 −1.570 −0.574 0.628 −0.319 0.731 0.617 −1.992 DRD3-Tango 0.079 −2.916 0.030 0.050 0.598 1.915 1.415 −2.126 DRD4-Tango −0.322 −3.000 −0.052 0.554 0.114 1.065 0.874 −1.032 DRD5-Tango −0.666 −1.197 −0.547 −0.687 −0.597 −0.141 0.364 −1.667 EDNRA-Tango −0.260 −0.197 −0.335 −0.551 −0.261 −0.258 0.135 −1.213 EDNRB-Tango 0.160 −0.503 0.127 −0.455 −0.182 −0.363 −0.363 −0.851 ELTD1-Tango −0.636 −0.325 −0.603 −1.290 −0.807 −0.414 −0.383 −1.309 F2R-Tango −0.198 −0.371 −0.185 −0.576 −0.236 −0.114 −0.497 −0.953 F2RL1-Tango −0.220 −0.207 −0.166 −0.730 0.177 −0.023 −0.361 −0.855 F2RL2-Tango −0.212 −0.580 −0.178 −0.541 0.025 −0.228 −0.482 −0.865 F2RL3-Tango −0.251 −0.594 −0.305 −0.776 −0.241 −0.140 −0.135 −0.966 FFA1-Tango 0.009 0.110 0.055 −0.349 −0.069 −0.607 −0.629 −1.057 FFA2-Tango −0.229 −0.732 −0.111 −0.575 −0.382 −0.175 −0.167 −1.365 FFA3-Tango −0.051 −0.682 −0.136 −0.078 0.032 0.020 0.175 −0.763 FPR1-Tango −1.079 −1.491 −0.924 −0.992 −0.511 0.074 0.153 −1.639 FPR2-Tango 1.469 −0.237 1.608 −0.251 0.535 0.420 0.286 −0.904 FPR3-Tango 0.165 −0.607 0.077 −0.660 −0.330 −0.062 −0.270 −1.221 FSHR-Tango −0.289 −1.236 −0.408 −0.479 −0.343 −0.382 −0.305 −0.682 GABBR1-Tango −0.067 −1.129 −0.132 −0.310 0.129 −0.260 −0.260 −0.577 GAL1-Tango −0.669 −0.357 0.387 0.430 0.019 −0.383 −0.596 −2.110 GAL2-Tango 0.302 −0.772 0.211 0.213 0.000 1.657 0.791 −0.823 GAL3-Tango −0.105 −0.888 0.054 −0.443 0.149 −0.645 −0.122 −0.731 GCGR-Tango −0.280 −0.415 −0.237 −0.435 −0.258 −0.471 −0.111 −0.713 GHRHR-Tango −0.302 −1.177 −0.302 0.053 −0.430 −0.575 −0.728 −1.271 GHSR-Tango −0.954 −0.425 −0.641 −0.684 −1.092 −0.086 −0.395 −1.579 GIPR-Tango −0.703 −0.835 −0.538 −0.324 −0.787 0.000 −0.385 −0.998 GLP1R-Tango −0.894 −0.759 −0.674 −0.333 −0.387 −0.099 −0.475 −1.094 GLP2R-Tango 0.395 0.014 0.475 0.339 −0.152 0.633 −0.100 −1.090 GNRHR-Tango 0.143 −0.628 −0.040 0.304 −0.173 0.420 0.094 −1.115 GPBA-Tango 0.713 −0.463 0.494 0.331 0.260 0.266 0.595 −1.291 GPER-Tango −0.461 −0.873 −0.447 −0.154 −0.104 0.038 0.038 −1.028 GPR1-Tango −0.189 −0.699 −0.064 −0.295 −0.556 −0.289 −0.387 −1.174 GPR101-Tango 0.355 −0.481 0.232 −0.354 −0.045 0.053 0.251 −0.734 GPR110-Tango −0.331 −0.858 −0.364 −0.717 −0.543 0.085 −0.024 −0.758 GPR111-Tango −0.292 −1.422 −0.311 −0.207 0.118 0.018 −0.069 −0.875 GPR113-Tango −0.487 −0.446 −0.371 −0.669 −1.174 0.149 −0.243 −0.952 GPR114-Tango −0.091 −0.667 −0.306 −0.102 −0.199 −0.107 0.082 −0.544 GPR115-Tango −0.279 −0.335 0.020 −0.601 −0.415 −0.440 −0.059 −0.626 GPR116-Tango −0.449 −0.432 −0.374 −0.452 −0.577 −0.253 −0.187 −0.800 GPR119-Tango −0.006 −0.788 0.010 −0.129 0.034 0.141 −0.073 −0.862 GPR12-Tango −0.241 −0.416 0.018 −0.133 −0.282 −0.182 −0.293 −0.915 GPR120-Tango 0.162 −0.728 0.246 0.045 0.063 −0.136 −0.355 −0.708 GPR123-Tango −0.384 −0.416 −0.020 0.196 −0.094 −0.212 0.218 −0.378 GPR124-Tango −0.237 −0.417 −0.193 0.079 −0.021 −0.233 0.289 −1.465 GPR125-Tango 0.239 −0.279 −0.021 −0.104 0.137 0.109 0.281 −0.712 GPR126-Tango −0.016 −1.030 0.086 −0.411 0.151 −0.184 −0.325 −0.765 GPR132-Tango −0.380 −1.091 −0.051 −0.452 −0.190 −0.353 −0.358 0.188 GPR133-Tango −0.242 −0.576 0.024 −0.323 −0.140 −0.251 −0.474 −0.365 GPR135-Tango −0.169 −1.208 −0.159 −0.092 0.101 0.117 −0.051 −0.499 GPR141-Tango −0.056 −0.276 0.079 −0.251 0.030 −0.385 −0.369 −0.688 GPR142-Tango −0.397 −0.404 −0.136 −0.185 −0.049 0.111 −0.065 −0.811 GPR143-Tango −0.225 −0.515 −0.333 0.027 0.147 −0.072 0.414 −1.074 GPR144-Tango −0.329 −0.537 −0.524 0.022 −0.317 −0.585 −0.585 −1.654 GPR146-Tango −0.118 −0.525 0.222 −0.037 −0.043 0.066 −0.472 −1.363 GPR148-Tango 1.087 −2.373 1.700 0.467 1.828 −0.485 0.147 −1.260 GPR149-Tango 0.419 −0.374 0.519 0.723 0.858 0.310 0.208 −1.836 GPR15-Tango −0.955 −0.303 −0.441 0.804 0.465 −0.369 0.196 −1.656 GPR150-Tango 0.558 −0.350 0.680 0.118 0.558 0.813 −0.122 −1.377 GPR151-Tango 0.456 0.240 0.781 0.319 0.749 −0.900 −0.942 −1.920 GPR152-Tango −0.089 −0.023 −0.060 0.034 −0.007 −0.471 −0.239 −0.642 GPR153-Tango 0.173 −0.334 0.189 0.144 0.132 −0.184 −0.323 −0.800 GPR156-Tango 0.095 −0.455 0.128 0.273 −0.035 0.078 0.007 −0.723 GPR157-Tango 0.114 −0.523 0.370 0.227 0.370 −0.113 −0.375 −0.765 GPR158-Tango 0.014 −0.358 0.136 0.103 0.506 −0.006 0.077 −0.558 GPR160-Tango 0.243 −0.038 0.313 0.311 0.574 −0.005 −0.284 −0.430 GPR161-Tango 0.347 −0.022 0.471 0.442 0.454 −0.392 −0.120 −0.484 GPR162-Tango −0.139 −1.232 0.034 −0.176 −0.009 −0.354 −0.136 −0.646 GPR17-Tango 0.189 0.326 0.265 0.412 −0.078 0.095 0.238 −1.204 GPR171-Tango 0.050 −0.297 0.207 0.583 0.078 0.013 −0.179 −0.806 GPR173-Tango 0.054 −0.231 0.467 0.082 0.196 −0.265 −0.155 −0.783 GPR174-Tango 0.029 0.068 0.543 0.145 0.086 −0.097 0.000 −0.753 GPR18-Tango 0.403 −0.008 0.609 0.668 0.510 −0.239 −0.224 −0.956 GPR182-Tango −0.019 −0.204 0.601 0.307 −0.052 −0.654 −0.519 −0.861 GPR183-Tango −0.170 −0.711 0.330 0.270 0.140 −0.460 −0.518 −0.678 GPR19-Tango −0.477 −0.372 0.175 0.189 0.002 −0.858 −0.941 −0.608 GPR20-Tango 0.675 −0.585 0.128 0.136 0.652 0.170 −0.368 −0.785 GPR21-Tango 0.271 −1.250 0.076 0.488 0.301 0.000 −0.345 −0.931 GPR22-Tango 0.109 −0.376 0.204 0.209 0.479 0.094 0.173 −0.508 GPR25-Tango −0.242 −1.126 0.169 0.147 0.320 0.000 0.000 −0.797 GPR26-Tango −0.013 −0.064 0.182 0.169 0.659 −0.214 −0.255 −1.004 GPR27-Tango −0.036 −0.745 0.236 0.402 0.150 0.468 0.303 −0.628 GPR3-Tango 0.229 0.113 0.330 −0.018 0.307 −0.195 0.025 −0.829 GPR31-Tango −0.148 −0.492 0.181 −0.006 0.193 −0.289 −0.080 −1.281 GPR32-Tango −0.402 −0.470 −0.276 −0.367 −0.775 −0.040 −0.040 −1.875 GPR34-Tango −0.246 −1.089 −0.068 −0.229 −0.455 0.039 −0.379 −1.474 GPR35-Tango −0.229 −0.608 −0.085 −0.158 −0.647 −0.245 0.060 −1.435 GPR37-Tango −0.307 −0.732 0.129 −0.131 −0.512 −0.110 −0.072 −1.072 GPR37L1-Tango −0.257 −0.241 −0.329 0.142 0.117 −0.249 −0.114 −0.873 GPR39-Tango −0.349 −0.500 −0.431 −0.481 −0.383 −0.756 −0.332 −0.762 GPR4-Tango −0.221 −0.602 −0.069 −0.015 −0.446 0.039 0.069 −0.599 GPR44-Tango −0.269 −0.792 −0.391 −0.182 −0.297 0.000 −0.126 −0.667 GPR45-Tango −0.649 −0.451 −0.549 −0.463 −0.757 −0.260 −0.824 −0.941 GPR50-Tango 0.265 −0.540 0.418 0.858 0.358 0.249 0.092 −1.234 GPR52-Tango 0.211 −0.971 0.165 0.406 −0.030 0.178 −0.135 −0.840 GPR55-Tango −0.293 −1.228 0.002 0.388 −0.192 −0.190 −0.328 −1.023 GPR56-Tango −0.116 −0.486 0.006 0.716 0.180 −0.384 0.222 −0.955 GPR6-Tango 0.186 −0.389 0.256 0.272 −0.160 −0.147 −0.876 −1.210 GPR61-Tango −0.224 −0.276 0.039 0.635 −0.162 −0.402 −0.646 −0.665 GPR62-Tango 0.027 −0.477 0.262 0.457 −0.062 −0.527 −0.740 −0.718 GPR63-Tango −0.063 −0.225 −0.027 −0.301 −0.236 −0.414 0.000 −0.268 GPR64-Tango −0.490 −0.569 −0.443 −0.049 −0.554 0.039 −0.040 −0.543 GPR65-Tango −0.182 −0.178 −0.043 −0.174 −0.155 −0.313 −0.441 −0.513 GPR68-Tango −0.597 −0.860 −0.571 −0.450 −0.366 −0.103 −0.546 −0.622 GPR75-Tango −0.147 −0.423 −0.062 0.360 0.185 −0.168 −0.309 −0.313 GPR77-Tango −0.999 −1.201 −0.605 −0.058 −0.818 −0.872 −0.941 −1.169 GPR78-Tango 0.104 −0.459 0.238 0.596 0.063 −0.755 −0.578 −0.623 GPR82-Tango −0.611 0.077 −0.175 −0.033 −0.569 −0.306 0.011 −1.337 GPR83-Tango −0.352 −0.444 −0.239 −0.017 −0.279 −0.176 −0.481 −0.740 GPR84-Tango −0.438 −0.220 −0.235 0.006 −0.420 −0.816 −0.813 −0.840 GPR85-Tango −0.231 −0.519 −0.199 0.368 −0.042 −0.443 −0.261 −0.814 GPR87-Tango −0.082 0.012 0.047 0.333 −0.268 −0.157 −0.524 −0.942 GPR88-Tango −0.044 −1.054 0.171 0.090 0.000 0.223 0.434 −0.796 GPR97-Tango −0.407 −0.779 −0.216 0.161 −0.540 −0.110 −0.767 −0.510 GPRC5A-Tango −0.665 −0.515 −0.287 0.093 −0.582 −0.711 −0.920 −0.738 GPRC5B-Tango −0.195 −0.397 0.017 0.233 −0.226 −0.780 −0.594 −0.565 GPRC5C-Tango −0.275 −0.214 −0.262 0.215 −0.222 −0.409 −0.387 −0.077 GPRC5D-Tango −0.426 −0.641 −0.478 −0.209 −0.451 0.000 −0.494 −0.493 GPRC6A-Tango −0.520 −0.671 −0.318 −0.186 −0.458 −0.559 −0.737 −0.653 GRM1-Tango −0.624 −0.498 −0.632 −0.060 −0.309 −0.324 −0.441 −0.750 GRM2-Tango −0.414 −1.138 −0.241 0.000 −0.466 −0.462 −0.510 −0.567 GRM4-Tango −0.637 −1.282 −0.434 −0.050 −0.478 −0.542 −0.288 −0.328 GRM5-Tango −0.139 −1.161 −0.093 −0.428 −0.024 −0.746 −0.589 0.143 GRM6-Tango −0.421 −0.620 −0.555 0.514 −0.501 −0.379 −0.404 −1.185 GRM7-Tango −0.769 −0.486 −0.655 −0.725 −1.030 −0.309 −0.099 −0.705 GRM8-Tango −0.663 −0.857 −0.505 −0.589 −0.886 −0.219 −0.147 −0.864 GRPR-Tango −0.295 −0.515 0.067 −0.227 −0.234 −0.094 −0.150 −0.518 HCA1-Tango −0.113 0.290 0.218 0.373 0.143 −0.399 −0.399 −0.825 HCA2-Tango −0.061 0.175 0.761 0.119 0.414 0.131 −0.087 −0.670 HCA3-Tango −0.108 0.178 −0.100 −0.082 0.056 0.235 0.033 −0.910 HCTR1-Tango 0.000 0.745 0.000 −0.181 0.258 0.369 −1.778 −1.122 HCTR2-Tango 0.435 0.000 −0.395 0.451 0.435 0.122 0.234 −0.754 HRH1-Tango 0.264 −0.194 0.486 −0.537 −0.113 −0.203 −0.060 −1.351 HRH2-Tango 0.154 −0.607 0.180 0.176 0.006 0.417 0.389 −0.707 HRH3-Tango −0.022 −0.744 −0.140 0.658 0.201 0.097 0.343 −0.793 HRH4-Tango 0.283 −0.675 0.300 0.164 0.361 0.659 0.431 −1.142 HTR1A-Tango 0.091 −0.340 0.269 0.044 0.174 0.174 0.000 −0.760 HTR1B-Tango 0.088 −0.426 0.093 −0.487 0.069 0.066 −0.102 −1.133 HTR1D-Tango −0.054 −0.272 0.088 −0.592 −0.060 −0.405 −0.254 −1.155 HTR1E-Tango 0.093 0.033 0.001 −0.006 −0.257 −0.411 −0.251 −0.895 HTR1F-Tango −0.531 −0.696 −0.349 −0.802 −0.806 −0.214 −0.214 −1.566 HTR2A-Tango −0.345 −1.112 −0.472 −0.453 −0.501 −0.065 −0.114 −0.780 HTR2B-Tango −0.176 −1.200 −0.097 −0.439 −0.213 −0.028 0.115 −0.743 HTR2C-Tango −0.233 −1.471 −0.262 −0.062 −0.318 −0.570 −0.432 −1.034 HTR4-Tango −0.240 −0.761 −0.159 −0.311 −0.622 0.080 0.070 −0.725 HTR5-Tango −0.504 −0.169 −0.085 −0.050 −0.968 0.205 0.227 −0.234 HTR6-Tango 0.313 −0.707 0.365 0.000 −0.307 −0.413 −0.476 0.061 HTR7-Tango 0.339 −2.319 0.395 0.630 0.501 −0.189 −0.416 0.539 KISSPEPTIN-R- −0.020 −0.596 −0.041 −0.234 −0.275 −0.065 −0.333 −0.995 Tango LHCGR-Tango −0.494 −0.183 −0.574 −0.357 −0.671 −0.149 −0.379 −0.810 LPAR1-Tango 0.027 −0.272 0.173 −0.419 −0.560 −0.273 −0.210 −0.669 LPAR2-Tango −0.271 −0.637 −0.144 −0.260 −0.235 −0.817 −1.056 −0.815 LPAR4-Tango −0.188 −0.694 −0.190 −0.111 −0.864 −0.747 −0.980 −1.101 LPAR5-Tango −0.180 −0.464 −0.046 0.006 −0.319 −0.155 −0.493 −0.946 LPAR6-Tango 0.243 0.370 0.333 0.161 0.174 −0.025 −0.098 −0.387 LTB4R-Tango 0.000 −0.020 0.211 0.414 −0.082 −0.133 −0.313 −0.887 LTB4R2B-Tango 0.073 0.011 0.461 −0.478 0.232 −0.190 −0.540 −0.912 MAS1-Tango −0.164 −0.496 0.220 −0.280 −0.244 −0.470 −0.717 −1.686 MAS1L-Tango −0.071 −0.678 0.151 −0.614 −0.313 −0.717 −0.826 −0.946 MC1R-Tango −0.228 −0.723 0.260 −0.122 −0.195 −0.276 −0.313 −0.767 MC2R-Tango −0.382 −1.036 −0.019 0.059 −0.382 0.458 0.393 −0.736 MC3R-Tango 0.013 −0.560 0.434 0.288 0.389 −0.540 −0.285 −0.608 MC4R-Tango −0.307 0.225 0.334 0.587 −0.648 −0.397 −0.295 −1.194 MC5R-Tango −0.079 0.096 0.774 0.261 −0.346 −0.672 −0.180 −1.253 MCHR1-Tango −0.476 −0.172 −0.551 0.032 0.068 −0.578 −0.174 −1.137 MCHR2-Tango −0.214 −0.962 −0.177 0.190 −0.167 0.026 −0.629 −0.896 MLNR-Tango 0.395 −0.691 0.010 0.295 0.135 −0.170 0.132 −0.817 MRGPRD-Tango −0.069 −0.196 −0.024 0.185 −0.317 −0.396 −0.321 −1.075 MRGPRE-Tango 0.043 −0.326 −0.165 0.488 −0.224 −0.190 −0.321 −0.510 MRGPRF-Tango −0.377 0.216 −0.203 0.496 −0.173 −0.183 −0.172 −0.620 MRGPRG-Tango −0.030 0.076 −0.240 −0.219 0.051 −0.398 −0.294 −0.127 MRGPRX1-Tango −0.272 0.453 −0.302 −0.290 −0.221 −0.137 −0.151 −0.473 MRGPRX2-Tango −0.183 −0.820 −0.127 0.048 −0.986 0.012 −0.404 −1.182 MRGPRX3-Tango −0.457 −1.941 −0.482 0.069 −0.661 −0.079 −0.298 −0.998 MRGPRX4-Tango −0.983 −1.258 −0.779 −0.836 −1.240 −0.312 −0.209 −1.770 MTNR1A-Tango −0.743 −0.621 −0.483 −0.265 −1.072 −0.737 −0.120 −1.790 MTNR1B-Tango −0.465 −0.334 −0.254 −0.090 −1.011 −0.188 −0.490 −1.645 NMBR-Tango −0.572 −0.320 −0.160 0.542 −0.594 −0.480 −0.664 −0.667 NMUR1-Tango −0.589 0.148 −0.206 −0.090 −0.820 −0.523 −0.650 −1.423 NMUR2-Tango −0.895 −0.290 −0.534 0.085 −0.681 −0.420 −0.122 −0.915 NPBW1-Tango 0.142 −1.887 0.080 0.437 0.059 0.133 0.403 −1.022 NPBW2-Tango −0.107 −1.538 −0.256 −0.594 −0.521 −0.245 −0.436 −1.406 NPFF1-Tango 0.056 −1.303 0.138 0.257 0.071 −0.506 −0.336 −0.925 NPFF2-Tango 0.118 −0.825 0.293 0.571 0.404 −0.229 −0.353 −0.544 NPS-Tango −0.311 −0.817 0.257 0.155 0.300 −0.427 −0.766 −0.951 NPY1R-Tango −0.015 −0.788 0.067 0.039 0.219 −0.632 −1.016 −0.831 NPY2R-Tango −0.214 −1.710 −0.189 0.357 −0.492 −0.543 −0.589 −0.386 NPY4R-Tango −0.240 −0.900 −0.429 0.295 −0.154 0.379 −0.411 −0.527 NPY5R-Tango −0.404 −1.029 −0.088 0.459 0.267 0.120 0.000 −0.761 NTSR1-Tango −0.910 −0.773 −0.197 −0.075 −0.292 −0.184 −0.606 −1.006 NTSR2-Tango −0.368 −0.326 −0.023 −0.464 0.239 −0.276 −0.222 −1.288 OPN3-Tango −0.290 −1.384 −0.246 0.492 −0.237 −0.518 −0.225 −1.073 OPN5-Tango −0.681 −0.698 −0.279 −0.339 −0.589 −0.369 −0.478 −1.546 OPRD1-Tango −0.617 −0.740 −0.445 −0.248 −0.463 0.142 −0.219 −1.299 OPRK1-Tango 0.539 −0.369 0.408 −0.094 0.541 −0.214 −0.473 −0.506 OPRL1-Tango 0.000 −0.793 0.031 −0.108 −0.024 0.376 −0.624 −0.455 OPRM1-Tango −0.383 −1.009 −0.369 0.117 −0.098 −0.903 −0.225 −0.883 OXER1-Tango −0.244 −0.093 −0.354 −0.182 0.000 −0.348 −0.402 −0.727 OXGR1-Tango −1.097 0.156 −0.718 −0.384 −1.154 −0.245 −0.620 −1.871 OXTR-Tango −0.192 −1.257 −0.124 0.109 −0.074 −0.191 −0.429 −0.946 P2RY1-Tango 0.518 −0.549 0.222 −0.569 −0.211 −0.266 −0.352 −1.305 P2RY10-Tango −0.227 −0.965 −0.138 −0.416 −0.210 −0.333 −0.314 −0.697 P2RY11-Tango −0.126 −0.878 −0.102 −0.623 −0.206 −0.807 −0.178 −0.881 P2RY12-Tango −0.618 −0.740 −0.278 0.099 −1.306 0.346 0.065 −1.075 P2RY13-Tango 0.144 −0.509 0.242 −0.068 −0.471 0.159 −0.096 −1.524 P2RY14-Tango −0.106 −1.020 −0.145 −0.120 −0.476 −0.115 0.122 −1.354 P2RY2-Tango −0.387 −0.826 0.038 −0.310 −0.547 −0.221 −0.424 −1.495 P2RY4-Tango −0.414 −0.734 −0.319 −0.571 −0.889 −0.129 −0.320 −1.464 P2RY6-Tango −0.224 −0.406 −0.089 −0.114 −0.243 0.103 0.035 −1.687 P2RY8-Tango −0.229 −0.569 −0.320 −0.418 −0.626 0.145 0.016 −0.779 PK1-Tango 0.078 −0.638 −0.024 0.079 −0.057 −0.493 −0.128 −0.831 PK2-Tango 0.045 0.087 −0.230 0.362 −0.073 −0.258 −0.258 −0.778 PRRP-Tango −0.181 −0.493 −0.013 0.320 −0.041 0.436 0.160 −1.008 PTAFR-Tango −0.181 −0.655 −0.071 0.233 −0.348 −0.125 0.070 −0.872 PTGDR-Tango −0.114 −1.135 −0.210 −0.181 −0.382 −0.166 −0.339 −0.890 PTGER1-Tango 0.084 −0.794 −0.093 0.074 0.103 0.085 −0.542 −0.720 PTGER2-Tango −0.118 −0.554 0.034 0.331 0.080 0.272 −0.054 −0.787 PTGER3-Tango −0.135 0.034 −0.100 −0.178 −0.574 −0.090 −0.173 −0.768 PTGER4-Tango 0.473 0.409 0.598 0.701 0.612 −0.114 −0.260 −0.325 PTGFR-Tango −0.036 −0.150 0.026 −0.427 −0.623 0.260 0.060 −0.976 PTGIR-Tango −0.084 0.616 0.381 0.155 −0.194 0.813 0.376 −0.586 PTH1R-Tango −0.356 −0.611 −0.290 0.063 −0.236 0.317 −0.267 −0.755 PTH2R-Tango −0.260 0.068 −0.522 0.165 −0.272 −0.281 −0.274 −0.741 QRFP-Tango −0.291 −0.367 −0.096 0.095 −0.102 −0.142 0.214 −0.851 RXFP1-Tango −0.117 −0.146 −0.280 −1.021 −0.244 −0.602 −0.326 −0.542 RXFP2-Tango −0.655 −0.229 −0.756 −0.678 −0.700 −0.067 0.019 −0.778 RXFP3-Tango −0.621 −0.227 −0.585 0.035 −0.595 −0.910 −0.768 −0.576 RXFP4-Tango −0.737 −0.270 −0.280 −0.145 −0.168 −0.317 −0.565 −0.567 S1PR1-Tango −0.058 1.015 −0.044 0.526 −0.246 −0.793 −0.458 −0.333 S1PR2-Tango −0.204 0.515 −0.106 −0.294 −0.598 −0.142 −0.519 −0.949 S1PR3-Tango 0.044 0.684 −0.171 0.158 −0.316 −0.545 −0.027 −0.074 S1PR4-Tango −0.732 0.235 −0.405 0.127 −1.071 −0.484 −0.211 −1.523 S1PR5-Tango −0.073 0.229 0.250 1.019 −0.087 −1.000 0.000 −0.748 SCTR-Tango 0.421 0.346 0.062 0.243 0.176 0.115 0.115 −0.872 SSTR1-Tango −0.539 0.613 −0.476 0.087 −0.030 −1.404 −0.170 −0.778 SSTR2-Tango −0.344 0.000 0.069 0.312 0.436 −1.766 −0.333 −0.503 SSTR3-Tango −0.025 −0.181 0.065 0.096 0.065 0.697 0.463 −0.615 SSTR4-Tango −0.378 −0.193 −0.554 0.510 −0.210 −0.322 0.243 −0.984 SSTR5-Tango 0.769 −0.678 0.804 1.110 −0.023 0.269 −0.111 −0.153 SUCNR1-Tango 0.363 −0.044 0.222 0.825 −0.070 0.097 0.160 3.083 TA1-Tango −0.142 −0.533 −0.113 0.787 0.016 0.288 0.384 −0.582 TAAR2-Tango −0.180 −0.413 0.016 0.036 −0.061 0.070 −0.304 −0.208 TAAR5-Tango −0.166 −0.892 −0.323 −0.223 −0.228 −0.513 −0.483 −1.021 TAAR6-Tango −0.488 −0.402 −0.598 −0.261 −0.629 −0.540 −0.652 −0.670 TAAR8-Tango −0.333 −0.323 −0.467 −0.184 −0.524 −0.130 −0.238 −0.364 TAAR9-Tango −0.457 −0.537 −0.362 −0.301 −0.314 −0.053 −0.104 −0.360 TACR1-Tango −0.272 −0.338 −0.127 −0.015 −0.482 −0.026 0.120 −0.686 TACR2-Tango 0.045 −0.081 0.039 0.111 −0.201 −0.491 −0.421 −0.523 TACR3-Tango −0.175 −0.311 −0.139 0.477 −0.632 −0.374 −0.382 −0.889 TBXA2R-Tango −0.262 −0.968 −0.402 0.233 −0.298 0.026 −0.728 −0.746 TSHR-Tango −0.179 −0.598 −0.404 −0.111 −0.154 −0.772 −1.092 −0.809 UTS2R-Tango −0.024 −0.216 0.043 0.201 −0.218 −0.969 −1.212 −0.870 VIPR1-Tango −0.505 −0.124 −0.478 0.582 −0.419 0.039 −0.353 −0.999 VIPR2-Tango −0.360 −0.455 0.874 1.247 1.049 1.505 1.675 −0.806 PRESTO-Tango Bacteroids Bacteroids Bacteroids Bacteroids Bacteroids Bacteroids Bacteroids Bacteroids with GMM fragilis-2# fragilis-3# fragilis-4# fragilis-5# fragilis-6# fragilis-7# fragilis-8# fragilis-9# ADCYAP1R1- −0.613 −0.388 −0.138 −0.993 −0.768 −0.554 −0.156 0.504 Tango ADORA1-Tango −1.597 −0.662 −2.070 −1.714 −0.999 −0.852 −1.339 −0.455 ADORA2A-Tango −1.013 −0.062 −0.967 −0.290 −0.172 0.256 −0.733 0.487 ADORA2B-Tango −0.541 0.007 0.157 −0.605 −0.202 0.084 −0.409 0.156 ADORA3-Tango −0.975 0.472 0.563 −0.607 −0.250 −0.198 −0.284 0.435 ADRA1A-Tango −0.619 0.040 −0.078 −0.797 −0.071 −0.060 −0.325 0.477 ADRA1B-Tango −1.350 −0.036 −0.108 −1.019 −0.269 −0.278 −0.410 −0.108 ADRA1D-Tango −0.913 −0.913 −0.712 −0.708 −0.431 −0.225 −0.754 −0.224 ADRA2A-Tango −0.346 1.298 −0.036 −1.064 −1.170 −0.488 −0.677 0.106 ADRA2B-Tango −1.585 −0.131 −1.251 −1.034 −1.287 −1.677 −0.958 −0.235 ADRA2C-Tango 0.313 0.255 0.196 −0.115 −0.678 −0.415 −0.345 −0.463 ADRB1-Tango −0.266 0.180 −0.106 −0.954 −0.564 −0.435 −0.426 −0.300 ADRB2-Tango −0.993 −0.241 −0.788 −1.068 −0.754 −0.595 −0.363 0.350 ADRB3-Tango −0.141 −0.174 −0.174 −0.361 −0.436 0.219 0.000 −1.094 AGTR1-Tango −0.517 −0.292 −0.508 −0.505 −0.056 0.041 0.184 0.742 AGTR2-Tango −0.455 0.149 0.099 −1.348 −0.359 −0.705 −0.341 −0.063 APJ-Tango −1.541 −0.211 −0.575 −1.221 −0.451 −0.655 −1.040 −0.277 AVPR1A-Tango −1.098 −0.034 −1.293 −0.863 −0.273 −0.021 −0.918 −0.436 AVPR1B-Tango −0.973 −0.053 −0.511 −1.277 −0.290 −0.557 −0.339 −0.147 AVPR2-Tango −1.156 −0.184 −0.755 −1.659 −0.685 −0.691 −1.075 −0.336 BB3-Tango −1.552 −0.789 −1.112 −1.397 −0.849 −0.734 −0.923 −1.273 BDKBR1-Tango −0.364 −0.146 −0.520 −0.947 −0.412 0.024 −0.746 −0.333 BDKBR2-Tango −0.717 0.266 −0.126 −1.209 −0.036 −0.107 0.034 0.052 C3AR1-Tango −0.755 −0.337 −0.455 −1.347 −0.678 −0.386 −0.420 −0.535 C5A-Tango −0.757 −0.002 −0.153 −0.612 0.254 −0.094 −0.122 −0.194 CALCRb-Tango −0.290 0.016 −0.380 −0.448 −0.034 −0.103 −0.397 −0.505 CALCRL-Tango 0.009 −0.009 −0.261 −0.265 −0.498 0.236 −0.354 −0.425 CASR-Tango −0.932 −0.286 −0.320 −0.928 −0.548 −0.086 −0.244 −1.303 CCKAR-Tango −0.819 −0.012 0.029 −0.654 −0.109 −0.051 −0.003 −0.422 CCKBR-Tango −0.777 −0.360 −0.427 −0.702 −0.366 −0.231 −0.732 −1.005 CCR10-Tango −0.813 −0.049 −0.511 −0.703 −0.724 −0.392 −0.744 −0.752 CCR2-Tango −0.849 −0.342 −0.449 −0.386 −0.135 −0.289 −0.606 −0.748 CCR3-Tango −1.572 −0.545 −1.136 −2.224 −0.064 −0.515 −0.689 0.801 CCR4-Tango −1.263 0.045 −0.242 −1.173 −0.408 0.314 0.032 −0.318 CCR5-Tango −0.841 0.016 −0.281 −0.930 −0.182 0.000 −0.139 0.227 CCR6-Tango −0.921 0.210 0.143 −1.218 0.193 −0.149 0.000 0.108 CCR7-Tango −1.052 −0.167 −0.447 −0.503 −0.847 0.360 0.120 0.108 CCR8-Tango −1.481 −0.030 −0.687 −0.553 −0.776 −0.435 −0.477 0.106 CCRL2-Tango −0.426 0.362 0.275 −0.126 −0.441 0.015 0.000 0.310 CD97-Tango −0.680 −0.435 0.089 −1.050 −0.985 −0.688 −1.284 −0.543 CHRM1-Tango −0.588 0.276 −0.153 0.040 0.195 −0.246 −0.754 −0.209 CHRM2-Tango −0.695 −0.209 −0.232 −0.846 −0.765 −0.525 −0.750 −1.267 CHRM3-Tango −0.222 0.039 −0.033 −0.503 −0.787 −0.111 −0.742 −0.226 CHRM4-Tango 0.036 −0.027 −0.183 −0.362 −0.324 0.126 0.214 −0.060 CHRM5-Tango −0.436 −0.148 −0.290 −0.982 −0.651 −0.264 0.220 −0.539 CMKLR1-Tango −0.431 −0.032 −0.326 −0.732 −0.230 −0.062 −0.331 −0.390 CNR1-Tango −0.867 0.046 −0.522 −0.855 −0.605 −0.209 −0.598 −0.189 CNR2-Tango −0.843 −0.053 −0.727 −0.876 −0.616 −0.224 −0.748 0.335 CRHR1-Tango −0.434 0.029 −0.229 −0.957 −0.186 −0.415 −1.211 0.675 CRHR2-Tango −0.348 0.262 0.039 −0.745 −0.011 −0.588 −0.475 0.905 CX3C1-Tango −0.750 −0.370 −0.775 −0.595 −0.105 −0.302 −0.230 −0.142 CXCR1-Tango −0.950 −0.551 −0.602 −0.235 −0.809 0.103 0.125 0.620 CXCR2-Tango 0.049 −0.043 −0.314 −0.613 −0.768 −0.804 −0.282 0.540 CXCR3-Tango −0.983 −0.090 −0.366 −0.578 −0.442 −0.309 −0.366 0.485 CXCR4-Tango −0.200 −0.027 −0.086 −1.078 −0.553 −0.231 0.026 0.541 CXCR5-Tango 0.231 0.221 0.147 −0.673 −0.322 0.105 −0.187 0.094 CXCR6-Tango −0.705 −0.459 −0.372 −0.871 −0.174 −1.007 −0.105 0.719 CXCR7-Tango −1.436 −0.724 −0.676 −1.624 −1.236 −1.089 −1.271 −0.121 CYSLTR1-Tango −1.168 −0.713 −0.698 −0.820 −0.337 −0.473 −0.074 0.253 CYSLTR2-Tango −0.490 0.532 −0.188 −0.075 −0.523 −0.097 −0.024 0.055 DRD1-Tango −2.149 −0.785 −1.190 −1.293 −1.293 −0.320 −0.110 0.795 DRD2-Tango −0.397 −0.101 1.657 −1.851 −1.222 −0.411 −0.726 −0.871 DRD3-Tango −0.824 −0.577 0.050 −0.577 −0.948 −0.434 0.537 0.565 DRD4-Tango 0.068 0.313 1.133 −0.824 −0.846 0.685 1.270 −0.667 DRD5-Tango −1.482 −0.585 −1.052 −0.604 −0.198 −0.086 −0.703 0.559 EDNRA-Tango −1.453 −0.341 −0.738 −0.682 −0.426 −0.150 −0.324 0.055 EDNRB-Tango −0.863 −0.337 −0.581 −0.309 −0.323 0.319 −0.278 −0.158 ELTD1-Tango −1.662 −1.508 −1.605 −1.213 −0.957 −0.460 −0.870 −1.027 F2R-Tango −1.622 −0.554 −1.437 −0.869 −0.230 −0.083 −0.163 0.071 F2RL1-Tango −1.453 −0.418 −0.795 −0.807 −0.396 −0.016 0.124 −0.079 F2RL2-Tango −1.546 0.068 −0.744 −1.147 −0.633 0.006 0.001 −0.227 F2RL3-Tango −1.595 −0.468 −0.934 −1.010 −0.423 −0.047 −0.307 −0.222 FFA1-Tango −0.952 −0.510 −0.639 −1.237 −0.662 −0.300 −0.393 −0.444 FFA2-Tango −0.904 −0.402 −0.706 −0.716 −1.011 0.141 −0.206 −0.175 FFA3-Tango −1.012 −0.014 −0.168 −0.881 −0.581 0.058 −0.011 0.096 FPR1-Tango −1.192 −1.543 −1.514 −1.531 −1.225 −0.994 −1.173 −1.003 FPR2-Tango −0.760 −0.243 −0.156 −0.407 −0.396 0.212 −0.518 0.051 FPR3-Tango −1.444 −0.546 −0.646 −1.103 −0.784 −0.632 −0.663 −0.215 FSHR-Tango −1.001 −0.256 −0.406 −0.636 −0.498 −0.149 −0.577 −0.423 GABBR1-Tango −0.483 −0.238 −0.601 −0.189 −0.341 0.110 −0.371 −0.308 GAL1-Tango −1.445 −0.933 −0.990 −2.013 −0.883 −0.629 −1.892 −1.470 GAL2-Tango −1.824 −0.497 −1.070 −0.761 −0.079 −0.753 −1.350 0.791 GAL3-Tango −1.367 −0.417 −0.969 −0.680 −0.523 −0.531 −1.148 −1.040 GCGR-Tango −1.473 −0.458 −0.771 −0.493 −0.505 −0.242 −1.136 −0.376 GHRHR-Tango −0.990 −0.422 −0.442 −1.014 −0.782 −0.505 −0.465 −0.671 GHSR-Tango −1.653 −0.830 −1.404 −2.119 −0.505 −0.645 −0.479 −0.313 GIPR-Tango −1.742 −1.021 −1.238 −1.203 −0.416 −0.200 −0.740 −0.122 GLP1R-Tango −1.757 −0.402 −0.864 −1.190 −0.423 −0.514 −1.059 −0.283 GLP2R-Tango −0.755 −0.052 −0.247 −1.477 0.023 −0.835 −0.496 0.080 GNRHR-Tango −0.518 0.264 0.000 −0.832 −0.209 0.062 −0.542 −0.377 GPBA-Tango −0.974 −0.204 −0.799 −1.196 −0.050 −0.890 −0.664 −0.345 GPER-Tango −0.798 −0.229 −0.628 −0.551 −0.156 −0.429 −0.350 −0.026 GPR1-Tango −1.148 −0.261 −0.493 −0.923 −0.755 −0.555 −0.372 −0.487 GPR101-Tango −0.699 −0.613 −0.530 −0.530 −0.482 −0.642 −0.370 −0.203 GPR110-Tango −1.108 −0.554 −0.685 −1.059 −0.482 −0.549 −0.699 −0.634 GPR111-Tango −0.585 −0.381 −0.567 −0.696 −0.107 −0.402 −1.052 −0.519 GPR113-Tango −1.051 −0.621 −0.792 −0.544 −0.735 −0.511 −0.540 0.092 GPR114-Tango −0.138 0.120 −0.005 −0.076 −0.460 0.076 −0.317 −0.227 GPR115-Tango −0.892 −0.458 −0.386 −0.651 −0.786 −0.356 −0.588 −0.812 GPR116-Tango −0.734 −0.238 −0.371 −1.076 −0.414 −0.402 −0.956 −0.861 GPR119-Tango −0.279 −0.108 −0.261 −0.679 −0.549 −0.189 −0.277 0.125 GPR12-Tango −0.901 −0.345 −0.504 −1.386 −0.599 −0.115 −0.108 −0.248 GPR120-Tango −0.190 0.045 −0.072 −0.436 −0.296 0.200 −0.214 −0.086 GPR123-Tango −0.740 0.598 0.199 −0.730 −0.218 −0.528 −0.073 0.487 GPR124-Tango −0.718 −0.081 −0.134 −1.772 −1.050 −0.036 −0.678 −0.184 GPR125-Tango −0.695 −0.447 −0.466 −1.631 −0.845 −0.744 −0.382 −0.297 GPR126-Tango −0.943 −0.531 −0.753 −1.358 −0.898 −0.352 −1.000 −0.603 GPR132-Tango −1.237 −0.078 −0.388 −1.443 −0.917 −0.025 −0.742 −0.411 GPR133-Tango −1.073 −0.218 −0.343 −1.196 −0.815 −0.200 −0.953 −0.617 GPR135-Tango −0.748 −0.407 −0.573 −0.867 −0.517 −0.432 −0.326 0.042 GPR141-Tango −0.641 −0.061 −0.182 −1.680 −0.439 0.164 −0.793 −0.402 GPR142-Tango −0.844 0.000 −0.447 −1.764 −1.128 −0.296 −0.874 −0.012 GPR143-Tango −1.436 −0.187 −0.328 −0.992 −0.717 −0.619 −0.410 −0.329 GPR144-Tango −0.627 −0.046 −0.443 −0.915 −0.708 −0.871 −1.148 −0.406 GPR146-Tango −0.471 0.846 0.451 −0.835 0.000 0.415 0.982 −0.217 GPR148-Tango −0.503 −0.181 −0.415 −1.273 0.866 −0.968 0.112 0.000 GPR149-Tango −1.331 −0.703 −1.102 −1.617 −1.541 −1.033 −1.138 0.060 GPR15-Tango −1.229 0.332 −0.708 −1.505 −0.828 −0.350 −0.923 0.455 GPR150-Tango −0.648 0.200 0.000 −1.307 −0.270 −0.255 −0.087 −0.255 GPR151-Tango −1.117 0.130 −0.348 −0.585 −0.694 −0.009 −0.162 −0.386 GPR152-Tango −0.556 0.184 0.144 −0.656 −0.607 −0.193 −0.613 −0.542 GPR153-Tango −0.847 −0.130 −0.071 −1.009 −0.697 −0.376 −0.118 −0.229 GPR156-Tango 0.021 0.166 0.329 −1.012 −0.547 −1.307 −1.071 −0.367 GPR157-Tango −0.613 0.033 −0.221 −0.695 −0.331 −0.348 −0.419 −0.361 GPR158-Tango −0.419 0.021 −0.141 −0.832 −0.258 −0.206 −0.833 −0.720 GPR160-Tango −0.347 0.365 0.296 −0.421 −0.650 0.011 −0.279 −0.046 GPR161-Tango −0.466 0.074 −0.236 −0.899 −0.467 −0.304 −0.135 0.060 GPR162-Tango −0.651 −0.294 −0.502 −0.704 −0.730 −0.313 −0.552 −0.354 GPR17-Tango −0.213 0.164 −0.060 −0.731 −0.902 −0.557 −0.116 −0.050 GPR171-Tango 0.263 0.606 0.485 −0.504 −0.688 0.085 −0.251 −0.247 GPR173-Tango −0.932 0.454 −0.162 −0.751 −0.316 −0.462 0.084 −0.098 GPR174-Tango −0.627 −0.018 −0.171 −0.906 −0.316 0.255 −0.110 0.451 GPR18-Tango −0.830 −0.108 −0.443 −0.738 −0.861 0.408 0.058 0.674 GPR182-Tango −0.502 0.152 −0.105 −1.003 −0.667 −0.194 0.038 −0.007 GPR183-Tango −0.556 0.045 −0.217 −0.480 −0.357 0.275 −0.194 0.018 GPR19-Tango −0.845 0.110 −0.273 −0.753 −0.514 0.256 −0.046 −0.461 GPR20-Tango −0.240 0.026 −0.335 −0.553 −0.204 1.216 0.170 0.104 GPR21-Tango −0.644 0.060 −0.355 −0.570 −0.344 −0.142 −0.468 0.160 GPR22-Tango −0.636 0.141 −0.046 −0.802 −0.146 −0.095 −0.286 0.064 GPR25-Tango −0.440 0.147 −0.331 −0.588 0.199 0.120 −0.427 −0.220 GPR26-Tango −1.145 −0.642 −0.657 −1.700 −0.318 −0.164 0.436 −0.564 GPR27-Tango −0.241 0.125 0.000 −0.560 0.221 0.160 −0.090 0.303 GPR3-Tango −0.724 −0.359 −0.384 −1.363 −0.779 −0.243 −0.507 0.105 GPR31-Tango −1.172 −0.481 −0.345 −1.237 −0.795 0.015 −0.436 −0.238 GPR32-Tango −1.449 −0.834 −0.958 −0.798 −0.128 −0.684 −1.154 −0.080 GPR34-Tango −1.050 −0.333 −0.720 −0.744 −0.340 −0.286 −0.771 0.000 GPR35-Tango −1.138 −0.229 −0.666 −1.689 −0.815 −0.476 −0.759 −0.147 GPR37-Tango −0.890 −0.011 −0.299 −1.424 −0.436 −0.282 −0.225 0.393 GPR37L1-Tango −0.572 0.017 −0.117 −1.042 −0.636 −0.240 −0.186 −0.249 GPR39-Tango −0.672 −0.368 −0.591 −0.958 −0.511 −0.325 −0.674 −0.342 GPR4-Tango −0.873 −0.251 −0.448 −1.189 −0.777 −0.370 −0.292 0.180 GPR44-Tango −0.405 −0.045 −0.369 −0.862 −0.504 −0.167 −0.445 −0.126 GPR45-Tango −1.108 −1.048 −0.672 −1.181 −0.124 −0.554 −0.756 −0.106 GPR50-Tango −0.572 0.405 0.124 −0.732 −0.026 −0.106 0.255 1.125 GPR52-Tango −0.589 0.144 −0.097 −1.097 −0.118 −0.386 −0.048 0.103 GPR55-Tango −0.337 0.433 0.111 −1.009 −0.159 −0.335 0.045 −0.009 GPR56-Tango −1.125 −0.033 −0.187 −1.623 −0.351 0.309 −0.016 0.116 GPR6-Tango −0.666 0.013 −0.195 −1.025 −0.231 0.164 0.285 0.353 GPR61-Tango −0.942 0.596 0.378 −0.985 −0.493 −0.131 0.043 0.478 GPR62-Tango −0.357 0.298 0.397 −0.442 −0.064 0.454 −0.081 −0.379 GPR63-Tango −0.450 0.080 −0.185 −0.338 −0.747 −0.552 −0.598 −0.696 GPR64-Tango −0.607 −0.181 −0.285 −0.535 −0.747 −0.542 −0.690 −0.326 GPR65-Tango −0.791 −0.170 −0.353 −0.437 −0.353 −0.551 −0.369 −0.240 GPR68-Tango −0.986 −0.261 −0.605 −0.654 −0.490 −0.634 −0.359 −0.302 GPR75-Tango −0.665 0.336 −0.093 −0.494 0.106 −0.270 −0.056 0.492 GPR77-Tango −0.804 −0.136 −0.371 −0.920 −0.425 −0.878 −0.674 −0.544 GPR78-Tango −0.924 0.212 −0.444 −0.725 −0.241 0.170 0.172 0.005 GPR82-Tango −1.790 −0.598 −1.036 −1.887 −0.570 −0.573 −0.636 0.171 GPR83-Tango −0.821 −0.002 0.007 −1.394 0.142 −0.303 −0.109 0.262 GPR84-Tango −1.221 −0.111 −0.228 −1.313 −0.268 −0.533 −0.685 0.093 GPR85-Tango −0.094 0.150 0.228 −0.586 0.136 0.016 −0.541 0.145 GPR87-Tango −0.672 0.114 0.048 −1.165 0.020 −0.571 −0.171 0.464 GPR88-Tango −0.396 0.084 −0.135 −0.358 0.236 −0.192 −0.179 0.386 GPR97-Tango −0.091 0.278 0.089 −0.372 −0.126 0.091 −0.213 −0.139 GPRC5A-Tango −1.108 0.002 −0.344 −1.420 −0.433 −0.290 −0.272 −0.219 GPRC5B-Tango −0.687 0.226 −0.149 −0.785 −0.316 0.128 −0.351 0.279 GPRC5C-Tango −0.788 0.446 0.331 −0.288 0.356 −0.029 −0.396 0.394 GPRC5D-Tango −0.408 0.146 0.049 −0.884 −0.054 −0.531 −0.539 −0.034 GPRC6A-Tango −0.614 0.123 0.018 −1.103 −0.198 −0.364 −0.937 −0.227 GRM1-Tango −0.748 −0.162 −0.245 −0.862 −0.286 −0.398 −0.951 −0.736 GRM2-Tango −0.400 0.160 −0.270 −0.588 −0.122 −0.158 −0.778 −0.407 GRM4-Tango −1.245 0.009 0.090 −0.806 −0.151 0.021 0.040 −0.185 GRM5-Tango −0.730 −0.186 −0.325 −1.140 −0.177 −0.280 −0.726 −0.714 GRM6-Tango −0.256 −0.452 −0.199 −1.069 −0.339 −0.835 −0.705 −0.115 GRM7-Tango −1.240 −0.881 −0.527 −2.117 0.029 −1.209 −1.571 −0.470 GRM8-Tango −0.754 −0.430 −0.411 −1.407 −0.489 −0.479 −0.469 −0.775 GRPR-Tango −0.849 0.110 −0.270 −1.394 0.312 −0.301 −0.156 0.658 HCA1-Tango −0.823 0.191 −0.315 −0.489 0.041 0.211 −0.144 0.028 HCA2-Tango −1.140 −0.114 −0.077 −0.930 0.210 −0.654 −0.404 0.233 HCA3-Tango −0.509 0.107 −0.299 −0.865 −0.178 0.479 −0.690 0.263 HCTR1-Tango −0.941 −0.941 −0.435 −0.437 0.125 0.350 0.255 −0.497 HCTR2-Tango −0.481 0.047 −0.415 −0.082 −0.719 −0.407 −0.255 0.000 HRH1-Tango −1.408 −0.709 −0.955 −1.530 −0.359 −0.840 −0.246 0.312 HRH2-Tango 0.077 0.182 0.083 −0.342 0.762 0.256 −0.081 0.520 HRH3-Tango 0.000 −0.132 −0.170 −0.523 −0.176 −0.913 −0.442 0.156 HRH4-Tango −0.339 −0.531 −0.740 −0.973 −0.390 −0.238 0.000 0.347 HTR1A-Tango −0.443 −0.107 −0.043 −1.248 0.084 0.348 0.355 0.374 HTR1B-Tango −0.925 −0.587 −0.633 −1.402 −0.484 −0.514 −0.034 0.500 HTR1D-Tango −1.051 −0.637 −0.806 −1.201 −0.607 −0.386 −0.317 0.359 HTR1E-Tango −0.712 −0.230 −0.456 −1.428 −0.189 −0.446 0.269 0.397 HTR1F-Tango −0.752 −0.227 −0.861 −1.305 −0.215 −0.538 −0.009 0.391 HTR2A-Tango −0.699 −0.276 −0.558 −0.886 −0.716 −0.254 −0.062 0.249 HTR2B-Tango −0.429 −0.229 −0.267 −0.899 −3.040 −0.033 −0.413 0.108 HTR2C-Tango −0.763 −0.141 −0.409 −0.777 −1.556 −0.084 −0.387 −0.308 HTR4-Tango −0.952 −0.061 −0.546 −0.180 −1.314 0.232 −0.091 0.595 HTR5-Tango −1.900 −0.214 −0.666 −1.230 −1.676 −0.425 0.047 1.159 HTR6-Tango −0.635 −0.118 −0.363 −0.942 −0.575 0.151 0.011 0.111 HTR7-Tango 0.157 0.531 0.166 −0.734 −2.230 −0.201 0.255 0.391 KISSPEPTIN-R- −0.792 −0.207 −0.833 −0.436 0.335 −0.219 0.326 0.122 Tango LHCGR-Tango −1.064 −0.401 −0.078 −0.932 0.230 −0.218 −0.310 −0.540 LPAR1-Tango −0.938 −0.325 −1.669 −1.210 0.061 −0.252 −0.407 −0.241 LPAR2-Tango −1.075 −0.230 −0.961 −0.892 −0.007 −0.292 −0.680 −0.427 LPAR4-Tango −1.394 −0.710 −1.799 −1.434 −0.153 −0.463 −0.393 −0.119 LPAR5-Tango −0.509 −0.082 0.128 −0.835 −0.225 −0.119 −0.289 0.043 LPAR6-Tango −0.915 0.192 0.873 −0.917 0.387 −0.192 0.085 0.470 LTB4R-Tango −0.507 0.299 0.358 −0.576 −0.089 0.076 −0.076 0.045 LTB4R2B-Tango −1.488 −0.478 −0.539 −0.639 −0.117 −0.024 −0.158 −0.078 MAS1-Tango −0.833 −0.170 −0.595 −1.116 −0.299 −0.149 −0.315 −0.060 MAS1L-Tango −1.096 −0.490 −0.513 −0.960 0.044 −0.665 −0.246 −0.673 MC1R-Tango −0.267 −0.012 −0.281 −0.331 −0.012 −0.161 0.009 −0.146 MC2R-Tango −0.314 0.126 −0.446 0.647 0.155 0.313 0.280 0.599 MC3R-Tango −0.974 0.423 −0.109 −0.669 0.499 −0.326 0.547 0.637 MC4R-Tango −0.877 0.400 −0.251 −0.445 0.110 0.396 0.086 0.629 MC5R-Tango −0.191 0.083 −0.395 −1.766 0.178 −0.471 0.226 0.514 MCHR1-Tango −0.674 −0.511 −0.523 −1.488 −1.331 −1.369 −0.696 −0.461 MCHR2-Tango −0.262 −0.199 −0.320 −1.075 −0.493 −0.390 0.102 −0.234 MLNR-Tango 0.112 0.226 −0.126 −0.615 −0.147 −0.272 −0.092 0.496 MRGPRD-Tango −1.014 −0.325 −0.629 −1.503 −0.843 −0.690 −0.195 −0.286 MRGPRE-Tango 0.054 0.255 0.153 −0.539 −0.467 −0.507 −0.209 0.126 MRGPRF-Tango −0.358 0.144 0.087 −0.812 −0.040 −0.526 −0.212 −0.216 MRGPRG-Tango −0.121 −0.180 0.034 −0.570 −0.660 −0.932 −0.040 −0.673 MRGPRX1-Tango −1.002 −0.168 −0.736 −1.630 −0.981 −0.766 0.008 −0.217 MRGPRX2-Tango −0.543 −0.140 −0.382 −1.123 −0.439 −0.626 −0.255 −0.282 MRGPRX3-Tango −0.206 −0.316 0.135 −0.646 −0.565 0.000 −0.392 −0.049 MRGPRX4-Tango −1.918 −0.845 −1.688 −1.776 −0.735 −1.283 −0.989 −0.495 MTNR1A-Tango −1.563 −0.377 −1.299 −1.323 0.232 −0.742 −0.643 0.205 MTNR1B-Tango −1.418 −0.411 −0.664 −2.198 −0.273 −0.972 −0.750 0.124 NMBR-Tango −0.385 −0.054 −0.228 −0.237 −0.011 −0.274 −0.355 0.490 NMUR1-Tango −0.893 −0.749 −0.861 −1.130 −0.044 0.821 −1.386 0.082 NMUR2-Tango −0.984 −0.045 −0.433 −1.916 −0.118 0.627 −1.040 0.628 NPBW1-Tango 0.097 −0.128 0.235 −0.923 −0.798 0.376 0.480 0.133 NPBW2-Tango −1.265 −0.893 −0.514 −1.797 −0.841 −1.129 −0.599 −1.352 NPFF1-Tango 0.293 0.519 0.235 −0.490 −0.124 −1.128 0.387 −0.662 NPFF2-Tango −0.720 0.249 −0.262 −0.608 0.337 −0.414 −0.146 −0.015 NPS-Tango −0.738 0.096 −0.125 −1.195 −0.112 −0.865 −0.045 −0.215 NPY1R-Tango −1.101 −0.192 −0.504 −0.544 0.089 −0.623 0.072 −0.426 NPY2R-Tango −0.579 0.477 0.523 −0.423 0.364 −0.163 0.069 −0.190 NPY4R-Tango −0.250 0.013 0.000 −0.643 −0.163 0.651 −0.577 0.493 NPY5R-Tango −0.644 0.453 0.091 −0.851 −0.033 −0.100 −0.450 −0.141 NTSR1-Tango −1.176 −0.459 −0.904 −1.128 −0.472 −0.529 −0.808 −0.189 NTSR2-Tango −1.130 −0.577 −1.055 −0.280 −0.043 0.156 −0.241 −0.308 OPN3-Tango −0.210 0.089 −0.197 −0.463 −0.346 −0.164 0.020 0.234 OPN5-Tango −1.856 −0.571 −1.120 −1.272 −0.513 −0.874 −0.839 −0.458 OPRD1-Tango −1.097 −0.610 −0.670 −1.089 −0.889 −0.628 −0.855 −0.055 OPRK1-Tango −1.039 −0.116 −0.272 −1.036 0.280 −0.218 0.236 0.157 OPRL1-Tango −0.704 −0.108 −0.220 −0.662 −0.125 −0.436 0.065 0.909 OPRM1-Tango −0.704 0.170 −0.730 −1.032 0.490 −0.488 −0.626 0.027 OXER1-Tango −0.892 −0.338 −0.092 −0.544 0.070 −0.424 −0.365 −0.458 OXGR1-Tango −1.415 −0.577 −0.714 −1.654 −0.507 −1.109 −0.737 −0.068 OXTR-Tango −0.376 0.090 −0.261 −0.705 0.040 0.144 −0.535 0.181 P2RY1-Tango −1.311 −0.490 −0.832 −1.657 −0.480 −0.430 −1.018 −0.184 P2RY10-Tango −0.882 −0.279 −0.392 −0.773 −0.070 −0.222 −0.421 0.112 P2RY11-Tango −1.192 −0.738 −0.674 −0.818 −0.478 0.090 −0.710 −0.550 P2RY12-Tango −1.497 −0.179 −0.320 −0.726 −0.533 −0.643 −1.517 −0.385 P2RY13-Tango −0.763 −0.138 0.040 −1.340 −0.727 −0.561 −0.478 0.155 P2RY14-Tango −0.932 −0.156 0.126 −1.009 −1.195 −0.787 −0.382 −0.227 P2RY2-Tango −1.224 −0.401 −0.343 −1.165 −0.734 −0.430 −0.710 −0.402 P2RY4-Tango −1.325 −0.703 −0.623 −1.881 −1.035 −0.524 −0.739 −0.169 P2RY6-Tango −0.945 −0.395 −0.364 −1.345 −1.136 −0.692 −0.739 −0.164 P2RY8-Tango −1.092 −0.147 −0.373 −1.250 −1.245 −0.832 −0.625 −0.056 PK1-Tango −0.579 −0.013 −0.183 −0.744 −0.635 −0.162 −0.043 0.118 PK2-Tango −0.436 0.183 −0.354 −0.354 −0.619 −0.374 −0.285 −0.346 PRRP-Tango −0.191 0.085 0.028 −0.442 −0.535 −0.280 −0.422 0.436 PTAFR-Tango −0.712 −0.055 0.033 −0.815 −0.660 −0.365 −0.707 0.397 PTGDR-Tango −0.773 −0.535 −0.499 −0.844 −0.350 −0.014 −0.377 −0.166 PTGER1-Tango −0.357 −0.085 −0.485 −0.202 −0.087 0.409 0.164 −0.220 PTGER2-Tango −0.241 −0.115 −0.241 −0.526 −0.076 −0.146 −0.327 0.761 PTGER3-Tango −0.806 −0.189 −0.147 −0.584 −0.436 −0.322 −0.745 0.367 PTGER4-Tango −0.529 0.173 −0.104 −0.710 −0.007 0.187 0.204 0.627 PTGFR-Tango −0.946 −0.409 −0.802 −0.900 −0.216 0.050 −0.322 0.435 PTGIR-Tango −0.280 −0.260 −0.665 −0.558 −0.842 0.788 −0.627 0.376 PTH1R-Tango −0.351 0.045 0.000 −0.638 −0.035 0.038 −0.702 0.412 PTH2R-Tango −0.794 −0.010 −0.023 −0.938 −1.281 −0.356 −0.769 0.177 QRFP-Tango −0.446 −0.085 −0.200 −0.815 −0.427 0.152 −0.758 0.073 RXFP1-Tango −1.146 −0.786 −0.599 −0.997 −0.759 −0.406 −0.693 −0.407 RXFP2-Tango −1.309 −0.275 −0.324 −1.204 −1.177 −0.447 −0.651 0.047 RXFP3-Tango −0.434 0.076 −0.260 −1.213 −0.498 −0.333 −1.138 −0.153 RXFP4-Tango −0.503 −0.478 −0.125 −0.391 −0.346 0.527 −0.888 0.260 S1PR1-Tango −0.776 0.141 0.089 0.452 −0.781 0.077 −0.416 0.074 S1PR2-Tango −1.680 −0.374 −0.508 −0.585 −0.785 0.230 −0.036 −0.031 S1PR3-Tango −1.172 0.308 −0.057 −0.190 −0.320 0.601 −0.170 0.253 S1PR4-Tango −1.560 −0.400 −0.636 −0.280 −1.012 −1.147 −0.813 −0.274 S1PR5-Tango −0.159 0.547 0.089 −0.094 −0.320 −0.087 −0.523 0.057 SCTR-Tango −0.384 −0.188 −0.016 0.000 0.000 −0.081 0.016 1.115 SSTR1-Tango −1.477 −0.050 −0.146 −1.433 −0.353 −0.105 −0.397 0.658 SSTR2-Tango −1.299 −0.404 −0.165 −0.545 −0.361 −0.325 −0.179 −0.766 SSTR3-Tango −0.650 0.288 −0.149 −0.118 0.212 0.000 0.303 1.250 SSTR4-Tango −0.153 0.496 0.265 −0.803 −0.638 −0.186 0.899 0.553 SSTR5-Tango 1.090 1.110 1.494 −0.153 −0.119 0.878 2.188 0.720 SUCNR1-Tango 3.693 3.615 3.868 1.245 2.167 2.407 3.913 2.771 TA1-Tango 0.380 0.537 0.651 −0.195 −0.878 −0.133 0.125 0.763 TAAR2-Tango −0.794 0.016 −0.118 −0.321 −0.345 −0.032 −0.394 0.596 TAAR5-Tango −0.163 −0.138 −0.739 −0.957 −0.503 −1.001 −0.351 −0.538 TAAR6-Tango −0.391 −0.252 −3.777 −1.014 −0.878 −0.931 −0.243 −0.475 TAAR8-Tango −0.447 −0.105 0.106 −0.774 −0.586 −0.714 −0.580 −0.727 TAAR9-Tango −0.807 −0.117 0.000 −0.978 −0.609 −0.636 −0.556 −0.532 TACR1-Tango −0.499 −0.095 0.035 −0.669 −0.999 −0.513 −0.632 0.094 TACR2-Tango −0.393 0.063 0.123 −0.327 −0.471 −0.450 −0.376 0.145 TACR3-Tango −0.642 0.244 −0.086 −0.550 −0.674 −0.333 −0.648 0.148 TBXA2R-Tango −0.387 0.160 −0.100 −0.753 −0.650 −0.177 −0.423 0.257 TSHR-Tango −1.215 −0.078 0.105 −1.284 −0.973 −0.602 −0.412 −0.331 UTS2R-Tango 0.041 0.000 0.133 −0.739 −0.366 0.093 −0.081 −1.286 VIPR1-Tango −0.681 −0.069 −0.098 −1.741 −0.142 −1.445 −0.264 −1.328 VIPR2-Tango 0.569 0.535 1.247 0.000 0.348 −0.145 1.636 1.133 PRESTO-Tango Bacteroides Bacteroides Bacteroides Bacteroides Bacteroides Bacteroides Bacteroides Bacteroides with GMM ovatus-1# ovatus-2# ovatus-3# uniformis-1# uniformis-2# uniformis-3# uniformis-4# spp-1# ADCYAP1R1- −0.685 −0.374 0.473 −0.193 −0.468 −0.420 −0.013 −0.679 Tango ADORA1-Tango −1.355 −1.171 −0.419 −0.775 −1.263 −1.014 −0.611 −1.225 ADORA2A-Tango −0.441 −0.472 −0.229 −0.322 −0.693 −0.817 −0.631 −0.034 ADORA2B-Tango −0.556 −0.365 −0.129 −0.173 −0.688 −0.392 −0.257 −0.288 ADORA3-Tango −0.114 −0.252 0.799 0.052 −0.566 −0.136 0.137 −0.239 ADRA1A-Tango −0.419 −0.632 0.157 −0.225 −0.539 −0.654 −0.351 −0.315 ADRA1B-Tango −0.475 −0.551 0.236 −0.304 −0.743 −0.806 −0.545 −0.475 ADRA1D-Tango −0.412 −0.676 −0.114 0.288 −0.222 −0.718 −1.361 −0.615 ADRA2A-Tango −0.110 −0.234 0.121 −0.971 −1.163 0.196 −0.211 −0.288 ADRA2B-Tango −1.034 −1.063 −0.222 −1.303 −1.500 −1.672 −1.226 −1.391 ADRA2C-Tango 0.281 −0.722 0.160 −0.931 −0.685 −1.046 −1.046 0.000 ADRB1-Tango −0.463 −0.470 0.477 −0.242 −0.605 −0.119 −0.264 −0.503 ADRB2-Tango −0.228 −0.044 0.344 0.603 −0.667 −0.425 −0.379 −0.613 ADRB3-Tango −0.516 −0.263 −0.167 −0.532 −1.063 −0.324 0.046 −0.733 AGTR1-Tango −0.324 0.000 0.157 −0.205 −0.415 −0.441 −0.316 −0.505 AGTR2-Tango −0.640 −0.241 0.835 0.098 −0.031 −0.386 −0.424 −0.519 APJ-Tango −0.241 −0.989 0.449 0.175 −0.238 −0.280 −0.381 −0.229 AVPR1A-Tango −0.059 −0.138 0.091 −0.140 −0.520 −0.128 0.180 −0.507 AVPR1B-Tango −0.642 −0.179 0.298 −0.639 −0.716 −0.478 −0.098 −0.493 AVPR2-Tango −0.586 −0.945 0.040 −1.100 −1.270 −0.189 −0.285 −0.687 BB3-Tango −0.528 −0.676 −0.049 −1.381 −1.605 −0.807 −0.835 −0.836 BDKBR1-Tango −0.733 −0.420 0.480 −0.835 −1.007 −0.234 −0.229 −0.219 BDKBR2-Tango −0.442 −0.095 0.769 0.341 −0.356 0.403 0.517 −0.477 C3AR1-Tango −1.002 −0.575 −0.192 −0.649 −0.939 −0.218 0.154 −0.793 C5A-Tango −0.262 −0.084 0.795 0.494 −0.586 −0.409 −0.182 −0.025 CALCRb-Tango −0.119 −0.133 −0.010 0.070 −0.745 −0.348 −0.325 −0.092 CALCRL-Tango 0.135 −0.189 −0.190 0.090 −0.245 −0.117 −0.132 0.018 CASR-Tango −0.413 0.082 0.433 −0.400 −0.854 −1.165 −0.518 −0.596 CCKAR-Tango −0.267 0.132 0.468 −0.070 −0.620 −0.335 0.048 −0.319 CCKBR-Tango −0.445 −0.788 0.072 −0.387 −0.779 −0.527 −0.518 −0.496 CCR10-Tango −0.616 −0.382 −0.162 −0.392 −0.786 −0.724 −0.284 −0.332 CCR2-Tango 0.049 −0.259 0.101 −0.171 −0.252 −0.468 −0.312 −0.231 CCR3-Tango −1.074 −0.635 0.109 0.377 −0.029 −0.649 −0.443 −0.786 CCR4-Tango −0.615 −0.197 0.805 0.094 −0.398 −1.014 −0.540 −0.096 CCR5-Tango −0.148 −0.013 0.193 −0.106 −0.660 −0.504 −0.270 −0.031 CCR6-Tango −0.654 0.010 0.221 0.812 0.256 −0.190 −0.090 −0.456 CCR7-Tango −0.353 −0.221 0.000 −0.125 −0.771 −0.504 −0.125 −0.159 CCR8-Tango −0.315 −0.350 0.429 0.086 −0.429 −0.842 −0.401 −0.058 CCRL2-Tango −0.387 −0.197 −0.393 −0.327 −0.585 −0.318 −0.631 −0.173 CD97-Tango −0.745 −0.939 0.290 −0.886 −0.886 −0.968 −0.752 −0.601 CHRM1-Tango −0.120 −0.564 0.113 −0.896 −0.645 −0.160 −0.482 0.192 CHRM2-Tango −0.610 −0.944 0.295 −0.793 −1.256 −0.353 −0.169 −0.435 CHRM3-Tango −0.230 −0.778 0.107 −0.598 −1.115 −0.939 −0.800 −0.516 CHRM4-Tango −0.293 0.047 0.285 −0.425 −0.572 −0.609 −0.261 −0.382 CHRM5-Tango −0.304 0.260 0.634 −0.365 −0.636 −0.149 −0.211 −0.254 CMKLR1-Tango −0.131 −0.074 0.403 −0.085 −0.444 −0.232 0.194 −0.224 CNR1-Tango −0.471 −0.316 0.709 −0.339 −0.821 −0.751 −0.213 −0.128 CNR2-Tango −0.526 −0.436 0.295 −0.392 −0.803 −0.405 −0.027 −0.549 CRHR1-Tango 0.000 −0.878 −0.234 −0.127 −0.127 −0.165 −0.335 −0.075 CRHR2-Tango −0.729 −0.503 0.538 −0.599 −0.371 −0.100 −0.049 −0.234 CX3C1-Tango −0.792 −0.712 0.552 −0.334 −0.298 0.091 0.037 −0.740 CXCR1-Tango −0.160 0.080 0.455 −0.985 −0.675 −0.402 −0.246 0.174 CXCR2-Tango −0.661 −0.149 0.429 −0.276 −0.309 −0.706 −0.462 −0.493 CXCR3-Tango −0.306 −0.545 0.337 −0.208 −0.252 −0.087 −0.072 −0.318 CXCR4-Tango −0.342 −0.083 0.332 −0.251 −0.298 −0.133 −0.057 −0.338 CXCR5-Tango −0.745 −0.254 0.247 0.031 −0.468 −0.271 −0.129 −0.525 CXCR6-Tango −0.434 −0.371 0.523 −0.457 −0.627 −0.144 0.142 −0.721 CXCR7-Tango −1.257 −1.182 −0.061 −1.029 −1.144 −1.119 −0.775 −1.011 CYSLTR1-Tango −0.807 −0.383 −0.091 −0.380 −0.487 −0.756 −0.787 −0.312 CYSLTR2-Tango 0.000 −0.405 0.416 −0.441 −0.463 −0.518 −0.568 −0.075 DRD1-Tango −0.174 −0.263 0.272 −0.426 −0.838 −0.710 −0.093 0.048 DRD2-Tango −0.823 −0.419 0.412 −1.616 −2.234 −1.672 −1.029 −1.010 DRD3-Tango 0.678 0.787 0.769 −2.481 −2.916 −1.449 −1.554 0.485 DRD4-Tango −1.322 0.404 0.126 −1.835 −2.867 −1.799 −1.174 −0.824 DRD5-Tango −0.478 −0.724 −0.111 −1.000 −0.715 −0.372 −0.189 −0.263 EDNRA-Tango −0.422 −0.340 0.541 −0.035 −0.606 −0.046 0.237 −0.385 EDNRB-Tango −0.180 −0.436 0.238 −0.670 −0.538 −0.238 −0.020 −0.167 ELTD1-Tango −0.722 −0.657 0.140 −0.497 −0.885 −0.952 −0.699 −0.819 F2R-Tango −0.503 −0.138 0.370 0.042 −0.428 −0.361 0.065 −0.540 F2RL1-Tango −0.448 0.249 0.682 0.064 −0.636 −0.319 −0.019 −0.296 F2RL2-Tango −0.682 0.208 0.341 −0.474 −0.989 −0.494 −0.311 −0.702 F2RL3-Tango −0.525 −0.284 0.336 −0.431 −0.718 −0.358 −0.200 −0.788 FFA1-Tango −0.520 −0.264 0.441 −0.147 −0.273 0.016 −0.075 −0.504 FFA2-Tango −0.228 −0.463 0.175 −0.581 −0.697 −0.321 −0.398 −0.615 FFA3-Tango −0.345 0.251 0.607 0.272 −0.721 −0.345 0.123 −0.234 FPR1-Tango −1.098 −0.567 0.070 −1.559 −1.536 −2.035 −1.119 −1.422 FPR2-Tango −0.372 −0.500 0.280 −0.709 −1.143 −0.289 0.022 −0.055 FPR3-Tango −0.462 −0.383 0.313 −0.576 −0.677 −0.154 −0.191 −0.471 FSHR-Tango −0.257 −0.437 0.314 −0.810 −0.850 −0.286 −0.199 −0.279 GABBR1-Tango −0.336 −0.242 0.124 −1.106 −0.863 −0.775 −0.243 −0.140 GAL1-Tango −0.733 −1.080 0.347 −0.819 −1.085 −0.836 −0.449 −1.328 GAL2-Tango −0.031 −0.719 0.357 −0.209 −1.040 −0.381 −0.171 −0.534 GAL3-Tango −0.827 −1.101 0.133 −0.385 −1.209 −0.836 0.075 −0.569 GCGR-Tango −0.189 −1.263 0.140 −0.878 −1.133 −0.645 −0.112 −0.289 GHRHR-Tango −0.492 −0.022 0.656 −0.457 −0.786 −0.135 0.198 −0.423 GHSR-Tango −1.211 −0.386 0.239 −0.628 −0.930 −0.212 −0.105 −1.295 GIPR-Tango −0.728 −0.177 −0.247 −0.574 −1.272 −0.214 0.000 −0.708 GLP1R-Tango −0.557 −0.491 0.246 −0.081 −1.289 −0.472 0.291 −0.863 GLP2R-Tango −0.041 −0.609 0.369 0.432 −0.216 −0.068 0.192 −0.189 GNRHR-Tango −0.160 −0.075 −0.114 −0.265 −0.988 −0.148 −0.191 0.018 GPBA-Tango −0.395 −1.122 0.242 −0.585 −0.628 −0.328 −0.822 −0.595 GPER-Tango −0.647 0.033 0.412 −0.618 −0.746 −0.408 −0.119 −0.760 GPR1-Tango −0.484 −0.527 0.118 −0.682 −1.545 −0.374 −0.579 −0.387 GPR101-Tango −0.222 −0.071 0.592 −0.662 −0.918 −0.047 −0.019 −0.246 GPR110-Tango −0.839 −0.718 0.103 −1.007 −0.897 −0.707 −0.667 −0.887 GPR111-Tango −0.711 −0.073 0.076 −1.015 −1.100 −0.260 −0.135 −0.430 GPR113-Tango −0.024 −0.540 −0.109 −0.292 −0.758 −0.461 0.110 −0.460 GPR114-Tango −0.069 −0.511 −0.071 −0.423 −0.941 0.000 −0.244 0.147 GPR115-Tango −0.637 −0.603 −0.063 −0.254 −0.717 −0.354 −0.375 −0.774 GPR116-Tango −0.800 −0.875 −0.163 −0.529 −0.780 −0.716 −0.544 −1.042 GPR119-Tango −0.376 −0.319 −0.269 −0.373 −0.610 −0.157 −0.181 −0.201 GPR12-Tango −0.706 −0.515 0.528 −0.558 −0.496 −0.070 0.023 −0.537 GPR120-Tango −0.177 −0.271 0.160 −0.622 −0.767 −0.634 −0.159 −0.292 GPR123-Tango −0.350 −0.315 0.250 −0.432 −0.517 −0.332 0.052 −0.232 GPR124-Tango −0.861 −0.467 0.046 −0.340 −0.653 −0.724 −0.715 −0.785 GPR125-Tango −1.163 −0.099 −0.130 −0.317 −0.497 −0.791 −0.824 −0.900 GPR126-Tango −0.795 −0.757 −0.191 −0.805 −0.958 −0.133 −0.365 −0.767 GPR132-Tango −0.813 −0.870 −0.048 −0.526 −0.795 −0.759 −0.553 −0.931 GPR133-Tango −0.579 −0.818 −0.318 −0.436 −0.668 −0.551 −0.419 −0.811 GPR135-Tango −0.488 −0.452 0.006 −0.241 −0.628 0.000 0.009 −0.745 GPR141-Tango −0.981 −0.691 0.720 −0.276 −0.619 −0.393 −0.451 −0.925 GPR142-Tango −0.887 −0.899 0.140 0.202 −0.646 −0.790 −0.696 −1.055 GPR143-Tango −0.453 −0.126 0.592 0.041 −1.160 −0.930 −0.848 −0.444 GPR144-Tango −0.605 −0.698 −0.102 −0.666 −1.387 −0.745 −0.870 −0.680 GPR146-Tango −0.358 0.000 0.358 −0.208 −1.270 0.068 −0.121 −0.204 GPR148-Tango −0.951 −0.122 −0.181 −1.511 −1.132 −0.828 −0.723 −1.025 GPR149-Tango −0.114 −0.050 0.334 −1.314 −1.257 −0.551 −0.855 −0.851 GPR15-Tango −0.120 −0.150 0.137 −0.229 −1.186 −0.052 0.000 −0.494 GPR150-Tango −0.420 −0.585 0.491 −0.531 −0.531 −0.093 −0.093 −0.220 GPR151-Tango −0.230 −0.019 0.730 −0.218 −0.857 −0.086 −0.086 −0.708 GPR152-Tango −0.166 −0.376 0.295 −0.061 −0.420 −0.560 −0.370 −0.301 GPR153-Tango −0.475 −0.140 0.324 −0.559 −0.699 −0.381 −0.225 −0.532 GPR156-Tango −0.480 −0.963 0.161 −0.413 −0.687 −0.127 −0.291 −0.571 GPR157-Tango −0.477 −0.145 0.201 0.053 −0.253 −0.556 −0.329 −0.214 GPR158-Tango 0.023 −0.987 0.080 −0.312 −0.606 −0.324 −0.108 −0.284 GPR160-Tango −0.176 −0.189 0.343 0.057 −0.581 −0.220 −0.202 −0.137 GPR161-Tango −0.483 0.026 0.356 −0.486 −0.651 −0.476 −0.568 −0.430 GPR162-Tango −0.456 −0.376 −0.072 −0.811 −1.017 −0.562 −0.466 −0.274 GPR17-Tango −0.335 −0.005 0.438 −0.036 −0.129 −0.697 −0.786 −0.472 GPR171-Tango −0.409 −0.215 0.415 −0.118 −0.550 −0.704 −0.797 −0.172 GPR173-Tango −0.400 0.084 0.138 −0.115 −0.720 −0.564 −0.795 −0.462 GPR174-Tango −0.328 −0.157 0.465 −0.327 −0.257 −0.490 −0.536 −0.301 GPR18-Tango −0.498 0.144 0.552 0.113 −0.409 −0.748 −0.362 −0.197 GPR182-Tango −0.471 0.061 0.134 −0.437 −0.482 −0.762 −0.676 −0.509 GPR183-Tango −0.404 −0.254 0.446 −0.014 −0.231 −0.197 −0.138 −0.352 GPR19-Tango −0.379 0.078 0.280 0.103 −0.489 −0.537 −0.352 −0.559 GPR20-Tango −0.191 0.700 0.000 −0.234 −0.415 −0.095 0.240 −0.463 GPR21-Tango 0.103 −0.515 −0.017 −0.459 −0.830 −0.175 −0.078 −0.312 GPR22-Tango −0.383 −0.005 0.527 0.017 −0.545 −0.263 −0.266 −0.277 GPR25-Tango −0.353 0.000 0.700 −0.484 −0.461 −0.404 −0.229 −0.060 GPR26-Tango −0.829 0.170 0.242 −0.064 −0.260 −0.066 −0.440 −1.003 GPR27-Tango 0.017 −0.187 0.709 −0.511 −0.435 −0.244 −0.200 0.034 GPR3-Tango −0.596 −0.447 0.794 −0.284 −0.342 0.212 0.056 −0.410 GPR31-Tango −0.979 −0.218 0.198 −0.425 −0.639 −0.315 0.018 −0.695 GPR32-Tango −0.503 −0.565 −0.184 −0.752 −0.426 −0.314 −0.248 −0.108 GPR34-Tango −0.798 −0.783 −0.011 −0.775 −1.040 −0.189 −0.016 −0.684 GPR35-Tango −0.812 −0.987 0.289 −0.761 −0.895 −0.244 −0.225 −0.826 GPR37-Tango −0.936 −0.508 0.389 −0.962 −1.204 −0.264 −0.035 −0.845 GPR37L1-Tango −0.534 −0.385 1.152 −0.180 −0.536 −0.194 −0.077 −0.688 GPR39-Tango −0.649 −0.736 0.558 −0.396 −0.466 −0.044 −0.248 −0.679 GPR4-Tango −0.781 −0.249 0.429 −0.799 −0.826 0.003 −0.200 −0.813 GPR44-Tango −0.433 −0.669 0.120 −0.637 −0.573 −0.136 0.000 −0.319 GPR45-Tango −0.315 −0.816 −0.194 −0.148 −0.335 −0.261 −0.097 −0.315 GPR50-Tango −0.567 0.378 0.391 0.238 −0.577 0.114 −0.216 −0.070 GPR52-Tango −0.454 0.013 0.619 −0.556 −1.358 0.103 −0.024 −0.345 GPR55-Tango −0.276 −0.134 0.281 −0.864 −1.792 0.069 −0.043 −0.622 GPR56-Tango −0.375 0.606 0.907 0.149 −0.960 0.115 −0.108 0.152 GPR6-Tango −0.487 −0.071 0.456 −0.183 −0.512 −0.294 −0.099 −0.362 GPR61-Tango −0.402 0.070 0.208 −0.180 −0.903 0.013 −0.040 −0.414 GPR62-Tango −0.539 0.000 −0.491 0.079 −0.330 0.158 0.133 −0.259 GPR63-Tango −0.046 −0.700 0.328 −0.480 −0.961 −0.757 −0.642 −0.238 GPR64-Tango −0.250 −0.573 −0.118 −1.095 −1.317 −0.330 −0.255 −0.372 GPR65-Tango −0.126 −0.266 0.149 −0.625 −0.790 −0.026 −0.140 −0.132 GPR68-Tango −0.617 −0.264 −0.165 −0.860 −1.123 −0.351 0.026 −0.627 GPR75-Tango −0.055 −0.027 0.200 −0.196 −0.889 −0.265 −0.142 0.114 GPR77-Tango −0.485 −0.591 0.087 −0.780 −1.437 −0.387 −0.227 −0.406 GPR78-Tango −0.006 0.298 0.495 −0.078 −0.822 −0.190 −0.116 0.174 GPR82-Tango −0.783 −0.095 0.148 −0.147 −0.703 −0.204 −0.242 −0.600 GPR83-Tango −0.600 −0.228 0.529 −0.670 −0.826 −0.532 −0.246 −0.686 GPR84-Tango −0.597 −0.485 0.497 −0.409 −0.936 −0.526 −0.318 −0.569 GPR85-Tango −0.274 −0.379 0.224 −0.339 −0.870 −0.396 −0.316 −0.284 GPR87-Tango −0.460 −0.258 0.313 −0.259 −0.389 −0.224 −0.254 −0.442 GPR88-Tango 0.112 −0.120 0.350 −0.651 −0.662 −0.263 −0.342 −0.242 GPR97-Tango −0.054 −0.253 −0.044 −0.681 −1.005 −0.550 −0.740 −0.120 GPRC5A-Tango −0.659 −0.158 0.182 −0.247 −0.907 −0.163 −0.277 −0.686 GPRC5B-Tango −0.374 −0.314 0.612 −0.083 −0.709 −0.511 −0.468 −0.281 GPRC5C-Tango −0.182 −0.319 0.708 0.054 −0.366 −0.381 −0.387 0.112 GPRC5D-Tango −0.314 −0.606 0.167 −0.569 −0.829 −0.553 −0.456 −0.443 GPRC6A-Tango −0.535 −0.943 0.353 −0.659 −0.729 −0.531 −0.302 −0.663 GRM1-Tango −0.706 −0.772 −0.079 −0.288 −0.731 −0.653 −0.375 −0.520 GRM2-Tango −0.313 −0.534 −0.049 −0.868 −1.341 −0.705 −0.533 −0.294 GRM4-Tango −0.361 −0.022 0.450 −0.783 −1.410 −0.543 −0.241 −0.433 GRM5-Tango −0.507 −0.782 −0.010 −1.035 −1.035 −0.305 −0.815 −0.638 GRM6-Tango −0.793 −0.769 0.277 −1.224 −0.806 −0.320 −0.391 −0.811 GRM7-Tango −0.744 −1.346 −0.119 −0.968 −1.321 −0.447 −0.741 −0.793 GRM8-Tango −0.773 −0.435 0.292 −0.762 −0.990 −0.966 −0.749 −0.590 GRPR-Tango −0.414 0.079 0.329 −0.615 −0.322 −0.331 −0.212 −0.431 HCA1-Tango −0.363 0.515 0.485 0.242 −0.327 −0.269 −0.515 −0.344 HCA2-Tango −0.505 −0.581 0.592 −0.223 −0.846 −0.914 −0.840 −0.216 HCA3-Tango −0.473 −0.442 0.557 0.280 −0.322 −0.785 −0.874 −0.585 HCTR1-Tango −0.553 0.675 0.626 0.585 −0.280 −0.160 0.434 −0.553 HCTR2-Tango 0.152 0.282 0.310 −0.112 −0.368 −0.497 −0.497 0.152 HRH1-Tango −0.533 −0.225 0.648 −0.625 −0.739 −1.182 −0.283 −0.667 HRH2-Tango −0.187 0.018 0.554 −0.329 −0.443 −0.558 −0.345 −0.282 HRH3-Tango −0.399 −0.380 0.645 −0.615 −0.615 −1.324 −1.039 −0.213 HRH4-Tango −0.697 −0.534 0.423 −0.750 −0.262 −0.468 −0.468 −0.697 HTR1A-Tango −0.404 0.094 0.500 −0.321 −0.561 −0.517 −0.620 −0.367 HTR1B-Tango −0.835 −0.189 0.611 −0.716 −0.686 −0.524 −0.464 −0.807 HTR1D-Tango −0.456 −0.418 0.476 −0.486 −0.470 −0.227 −0.364 −0.401 HTR1E-Tango −0.660 0.122 0.572 −0.359 −0.509 −0.518 −0.350 −0.815 HTR1F-Tango −0.510 0.029 −0.088 −0.398 −0.577 −1.011 −0.398 −0.542 HTR2A-Tango −0.341 −0.224 0.168 −0.723 −0.832 −1.272 −0.861 −0.398 HTR2B-Tango −0.442 −0.528 0.240 −0.959 −1.113 −0.625 −0.546 −0.474 HTR2C-Tango −0.265 −0.203 0.172 −0.671 −0.933 −0.662 −0.924 −0.255 HTR4-Tango 0.014 0.194 0.591 −0.475 −0.820 −0.177 −0.081 0.302 HTR5-Tango −0.669 0.221 0.384 0.000 −0.655 −0.038 0.195 −0.620 HTR6-Tango −0.597 −0.136 0.464 −0.268 −1.000 −0.736 −0.798 −0.401 HTR7-Tango −0.662 0.055 −0.193 −0.699 −1.058 −0.204 −0.499 −0.433 KISSPEPTIN-R- −0.094 −0.154 0.075 −0.068 −0.531 0.263 −0.282 0.108 Tango LHCGR-Tango −0.555 −0.202 0.547 0.054 −0.183 −1.304 −0.702 −0.828 LPAR1-Tango −0.227 −0.289 0.840 −0.363 −0.985 −0.518 −0.225 −0.787 LPAR2-Tango −0.542 −0.451 0.535 −0.149 −0.814 −0.681 −0.588 −0.671 LPAR4-Tango −0.838 −0.261 0.406 −0.409 −0.551 −0.870 −1.079 −0.718 LPAR5-Tango −0.670 −0.349 0.422 −0.599 −0.578 −1.000 −1.336 −0.356 LPAR6-Tango −0.594 0.281 0.516 0.037 −0.407 −1.250 −0.864 −0.395 LTB4R-Tango −0.194 0.097 0.426 0.180 −0.588 −0.520 −0.482 −0.239 LTB4R2B-Tango −0.371 −0.255 0.672 0.096 −0.569 −0.766 −0.444 −0.490 MAS1-Tango −0.463 −0.211 0.454 −0.639 −0.649 −0.274 −0.568 −0.311 MAS1L-Tango −0.486 −0.207 0.504 −0.728 −1.190 −0.703 −0.517 −0.398 MC1R-Tango −0.149 −0.183 0.266 −0.810 −0.670 −0.352 −0.466 −0.043 MC2R-Tango 0.715 0.147 0.569 −0.585 −0.982 0.144 −0.410 0.160 MC3R-Tango 0.256 0.529 1.348 0.516 −0.560 −1.064 −0.047 0.223 MC4R-Tango −0.445 0.230 0.741 0.225 −0.229 −0.031 −0.660 −0.286 MC5R-Tango −0.553 0.012 0.539 −0.189 −0.289 0.160 0.020 −0.617 MCHR1-Tango −0.853 −0.099 −0.157 −0.373 −0.561 −0.988 −0.951 −1.223 MCHR2-Tango −0.201 −0.158 −0.185 −0.604 −0.735 −0.705 −0.605 −0.609 MLNR-Tango −0.464 −0.265 0.110 −0.734 −0.883 −0.152 −0.727 −0.332 MRGPRD-Tango −0.481 −0.156 0.120 −0.539 −0.644 −0.887 −1.018 −0.578 MRGPRE-Tango −0.091 −0.420 0.298 −0.493 −0.837 −0.537 −0.751 −0.178 MRGPRF-Tango −0.595 −0.456 0.340 −0.614 −0.937 −0.435 −0.505 −0.320 MRGPRG-Tango −0.514 −0.209 0.403 −0.588 −0.988 −0.365 −0.628 −0.507 MRGPRX1-Tango −0.863 −0.133 0.295 −0.111 −0.771 −0.867 −0.498 −1.056 MRGPRX2-Tango −0.317 −0.418 −0.091 −0.977 −1.366 −1.446 −1.373 −0.400 MRGPRX3-Tango −0.197 −0.133 −0.079 −0.485 −1.000 −0.788 −0.968 −0.621 MRGPRX4-Tango −0.685 −0.420 0.312 −1.005 −1.548 −0.619 −0.452 −1.226 MTNR1A-Tango −0.301 −0.476 0.374 −0.762 −1.168 0.000 0.140 −0.119 MTNR1B-Tango −0.613 −0.735 0.051 −0.639 −0.756 −1.114 −0.816 0.931 NMBR-Tango 0.444 0.063 0.273 −0.480 −0.805 0.109 −0.100 0.479 NMUR1-Tango −0.626 −0.520 0.174 −0.087 −0.315 0.317 −0.341 −0.785 NMUR2-Tango −0.966 −0.642 0.533 0.000 −0.409 −0.170 −0.082 −1.118 NPBW1-Tango −0.298 0.260 −0.366 −0.640 −1.887 −0.350 −0.115 −0.554 NPBW2-Tango −0.716 −0.608 −0.976 −1.473 −1.619 −0.739 −0.776 −0.975 NPFF1-Tango −0.031 −0.350 0.160 −0.675 −1.663 −0.345 −0.467 −0.261 NPFF2-Tango −0.153 −0.439 0.363 0.227 −0.520 −0.028 0.023 −0.326 NPS-Tango −0.672 −0.015 −0.360 −0.133 −0.853 −0.422 −0.211 −0.722 NPY1R-Tango 0.000 0.061 −0.223 0.074 −0.706 −0.428 −0.193 −0.348 NPY2R-Tango −0.367 −0.255 0.169 −0.358 −1.109 −0.289 −0.346 −0.196 NPY4R-Tango −0.774 −0.149 −0.316 −0.585 −0.954 0.000 0.000 −0.798 NPY5R-Tango −0.126 −0.861 0.358 −0.509 −1.069 −0.763 −1.169 −0.325 NTSR1-Tango 0.036 −0.971 0.312 −0.915 −1.748 −0.408 −0.488 −0.298 NTSR2-Tango −0.221 −0.693 −0.110 −0.119 −1.082 −0.841 −0.743 −0.413 OPN3-Tango 0.267 0.212 0.425 −0.857 −1.066 −0.231 −0.357 −0.148 OPN5-Tango 0.207 −1.096 0.469 −0.813 −1.785 −1.037 −0.828 −0.543 OPRD1-Tango −1.027 −0.959 −0.352 −0.628 −0.582 −0.754 −0.637 −1.013 OPRK1-Tango −0.625 −0.186 0.604 −0.182 −0.501 −0.049 −0.054 −0.275 OPRL1-Tango −0.612 −0.128 0.241 −0.501 −0.857 −0.336 −0.411 −0.303 OPRM1-Tango −0.417 −0.334 0.055 −0.291 −0.599 −0.520 −0.054 −0.257 OXER1-Tango −0.150 −0.400 0.282 −0.558 −0.899 −0.253 −0.202 −0.191 OXGR1-Tango −0.657 −0.915 0.087 −0.623 −0.806 −0.686 −0.706 −0.608 OXTR-Tango 0.011 −0.729 0.307 −1.000 −1.363 −0.160 −0.256 −0.033 P2RY1-Tango −0.594 −1.031 0.220 −1.536 −1.571 −1.299 −1.160 −0.998 P2RY10-Tango −0.465 −0.742 0.275 −0.887 −0.787 −0.687 −0.543 −0.381 P2RY11-Tango −0.258 −0.995 0.254 −1.043 −1.213 −0.425 −0.410 −0.335 P2RY12-Tango −0.394 −1.488 0.160 −1.541 −2.094 −0.786 −0.717 −0.339 P2RY13-Tango −0.299 −0.535 0.492 −0.286 −0.799 −0.356 −0.336 −0.661 P2RY14-Tango −0.544 −0.491 0.281 −1.144 −1.302 −0.467 −0.533 −0.554 P2RY2-Tango −0.668 −0.910 0.202 −1.215 −1.272 −0.946 −0.943 −0.739 P2RY4-Tango −0.972 −0.813 0.152 −1.634 −1.512 −0.853 −0.805 −1.263 P2RY6-Tango −0.667 −0.812 0.282 −1.118 −1.480 −0.761 −0.786 −0.956 P2RY8-Tango −0.981 −0.888 0.105 −0.555 −1.028 −0.491 −0.414 −1.094 PK1-Tango −0.341 0.047 0.142 −0.608 −0.893 −0.814 −0.795 −0.330 PK2-Tango 0.000 0.044 0.234 −0.126 −0.281 −0.485 0.071 0.284 PRRP-Tango −0.141 −0.436 0.413 −0.732 −1.353 −0.529 −0.529 −0.016 PTAFR-Tango −0.155 −0.823 0.285 −1.206 −1.178 −0.838 −1.238 −0.370 PTGDR-Tango −0.159 −0.633 0.096 −1.071 −1.071 −0.643 −0.909 −0.422 PTGER1-Tango −0.092 0.164 0.000 −0.858 −0.573 −0.043 −0.165 0.065 PTGER2-Tango −0.296 −0.543 0.604 −0.241 −0.358 0.295 0.361 −0.184 PTGER3-Tango 0.123 −0.462 0.488 −0.436 −0.647 −0.882 −0.758 −0.210 PTGER4-Tango 0.023 0.503 0.842 0.000 −0.677 0.080 0.011 0.278 PTGFR-Tango −0.435 −0.387 0.611 −0.048 −0.420 −0.730 −0.458 −0.527 PTGIR-Tango −0.133 −0.193 −0.874 −0.521 −0.471 −1.866 −0.670 −0.382 PTH1R-Tango −0.498 −0.521 0.381 −0.737 −1.100 −0.230 −0.042 −0.306 PTH2R-Tango −0.641 −0.890 0.254 −0.627 −0.932 −0.390 −0.665 −0.363 QRFP-Tango −0.479 −0.381 0.393 −0.677 −0.487 −0.384 −0.188 −0.402 RXFP1-Tango −0.434 −0.336 −0.156 −0.664 −0.874 −0.956 −1.135 −0.458 RXFP2-Tango −0.844 −0.782 0.370 −0.691 −1.194 −0.770 −0.679 −0.834 RXFP3-Tango −0.784 −0.372 −0.151 −0.190 −0.335 −0.141 −0.786 −0.792 RXFP4-Tango 0.054 −0.840 0.216 0.000 0.102 −0.156 −0.156 −0.057 S1PR1-Tango 0.604 −0.659 1.282 0.619 0.369 −0.107 −0.605 0.505 S1PR2-Tango 0.687 −0.106 0.503 0.181 0.144 −0.729 −1.087 0.076 S1PR3-Tango 0.232 0.106 0.657 0.465 0.364 −0.200 −0.234 −0.258 S1PR4-Tango 0.383 −0.726 0.392 −0.425 −0.111 −1.035 −0.822 −0.164 S1PR5-Tango 0.326 −0.553 −0.126 −0.607 −0.220 −0.627 −0.521 −0.216 SCTR-Tango 0.670 0.063 −0.009 −0.156 −0.076 −0.335 −0.302 0.117 SSTR1-Tango −0.930 0.176 0.675 0.145 0.345 0.244 0.563 −0.832 SSTR2-Tango 0.531 0.037 0.519 0.705 −0.170 −0.661 −0.415 0.655 SSTR3-Tango 0.328 0.625 0.592 0.913 0.122 −0.415 −0.819 0.585 SSTR4-Tango −0.685 0.000 −0.186 −0.102 0.095 −0.665 −0.665 0.077 SSTR5-Tango 0.260 1.793 0.511 0.564 0.347 0.563 0.048 0.451 SUCNR1-Tango 2.335 3.592 3.198 2.538 2.624 2.500 2.186 2.453 TA1-Tango −0.423 −0.020 0.259 −0.365 −0.651 −0.429 −0.509 −0.150 TAAR2-Tango −0.141 −0.466 0.143 0.016 0.060 −0.673 −0.739 −0.029 TAAR5-Tango −0.517 −0.539 −0.381 −0.477 −0.442 −0.230 −0.541 −0.566 TAAR6-Tango −0.683 −0.380 −0.559 −0.662 −0.730 −0.342 −0.604 −0.901 TAAR8-Tango −0.336 −0.718 −0.071 −0.642 −0.503 −0.054 −0.085 −0.757 TAAR9-Tango −0.714 −0.616 0.266 −0.282 −0.952 −0.199 −0.217 −0.794 TACR1-Tango −0.516 −0.521 −0.092 −0.596 −1.064 −0.416 −0.421 −0.477 TACR2-Tango −0.245 −0.340 0.696 −0.049 −0.641 −0.544 −0.571 −0.010 TACR3-Tango −0.353 −0.304 0.319 −0.325 −0.938 −1.104 −0.875 −0.100 TBXA2R-Tango 0.040 −0.587 0.176 −0.531 −0.766 0.154 −0.672 0.120 TSHR-Tango −0.810 −0.412 −0.306 −0.906 −1.087 −0.414 −0.414 −0.674 UTS2R-Tango −0.144 0.086 −0.288 0.369 −0.982 −0.260 0.050 −0.334 VIPR1-Tango −0.863 −0.594 0.232 −0.550 −1.093 −1.188 −0.809 −0.767 VIPR2-Tango −0.333 0.990 1.354 −0.143 −0.503 −1.527 −0.645 −1.000 PRESTO-Tango Bacteroides Bacteroides Bacteroides Bacteroides Bacteroides Bacteroides Bacteroides Parabacteroides with GMM spp-2# spp-3# spp-4# spp-5# UC-1# UC-2# UC-3# distasonis-1# ADCYAP1R1- −0.583 −0.890 −0.495 −0.229 0.167 −0.190 −1.456 0.551 Tango ADORA1-Tango −0.995 −0.951 −0.768 −0.832 −0.621 −0.594 −0.921 −0.598 ADORA2A-Tango −0.225 −0.025 −0.533 −0.442 −0.387 −1.154 −0.052 −0.025 ADORA2B-Tango −0.365 −0.066 −0.303 −0.367 −0.281 −0.751 −0.215 −0.318 ADORA3-Tango −0.153 0.650 −0.387 −0.051 0.266 0.003 −0.499 0.019 ADRA1A-Tango −0.120 0.161 −0.681 0.000 −0.055 −0.355 −0.619 −0.142 ADRA1B-Tango −0.363 −0.254 −0.590 −0.368 0.032 −0.732 −0.501 −0.584 ADRA1D-Tango −0.549 −0.345 −0.521 −0.009 −0.616 0.018 −0.616 0.356 ADRA2A-Tango −0.430 −0.015 −0.197 −1.052 0.185 −1.309 −0.429 −0.357 ADRA2B-Tango −0.859 −1.026 −1.277 −1.103 −0.834 −1.144 −0.995 −0.383 ADRA2C-Tango −0.678 −0.077 −0.970 −0.208 −0.405 −1.036 0.037 −0.246 ADRB1-Tango −0.224 −0.188 0.354 −0.642 0.659 −0.705 −0.385 0.032 ADRB2-Tango −0.453 −0.974 −0.363 0.199 −0.170 0.333 −0.423 0.000 ADRB3-Tango 0.000 −1.000 −0.165 −0.405 −0.213 −0.405 −0.234 −0.397 AGTR1-Tango −0.244 0.147 −0.016 0.079 0.213 −0.415 0.226 0.409 AGTR2-Tango 0.039 0.264 −0.234 0.180 0.344 0.101 −0.567 −0.132 APJ-Tango −0.635 −0.141 −0.862 −0.215 −0.829 0.000 −0.628 −0.339 AVPR1A-Tango −0.642 0.034 −0.459 0.018 −0.290 −0.667 −0.023 −0.355 AVPR1B-Tango −0.496 −0.150 −0.489 −0.349 −0.112 −0.816 −0.111 0.040 AVPR2-Tango −0.665 −0.360 −0.814 −1.037 −0.651 −0.762 −0.501 −1.019 BB3-Tango −0.759 −0.770 −0.730 −1.178 −0.432 −0.949 −0.792 −0.617 BDKBR1-Tango 0.099 0.050 −0.588 −0.564 −0.259 −0.875 −0.180 −0.341 BDKBR2-Tango −0.166 0.555 −0.089 0.332 0.252 0.084 −0.283 −0.081 C3AR1-Tango −0.577 −0.497 −0.593 −0.612 −0.140 −0.809 −0.760 −0.711 C5A-Tango 0.150 0.123 −0.312 0.254 0.066 −0.034 −0.060 −0.278 CALCRb-Tango −0.163 −0.062 −0.149 −0.445 −0.256 −0.680 −0.376 −0.046 CALCRL-Tango −0.087 0.027 −0.454 0.127 −0.491 −0.569 −0.056 −0.206 CASR-Tango −0.481 −0.593 −0.378 −0.400 −0.265 −0.467 −0.082 −1.060 CCKAR-Tango −0.157 −0.228 −0.204 −0.119 0.217 −0.301 0.093 0.107 CCKBR-Tango −0.276 −0.336 −0.726 −0.266 −0.410 −0.351 −0.344 −0.936 CCR10-Tango −0.589 −0.511 −0.212 −0.531 −0.396 −0.622 −0.513 −0.806 CCR2-Tango 0.248 0.106 −0.671 0.184 −0.575 −0.252 −0.789 −1.661 CCR3-Tango 0.089 0.110 −0.197 0.313 0.080 −0.021 −0.859 −0.720 CCR4-Tango −0.362 −0.343 −0.383 −0.124 −0.078 −0.101 −0.650 −0.143 CCR5-Tango 0.073 0.219 −0.088 −0.012 0.095 −0.349 −0.180 0.280 CCR6-Tango 0.498 0.123 0.304 0.574 0.633 0.206 −0.201 0.376 CCR7-Tango −0.378 −0.470 −0.054 −0.125 0.120 −0.771 −0.497 −0.330 CCR8-Tango −0.265 −0.212 −0.300 −0.284 −0.187 −0.284 −0.708 0.181 CCRL2-Tango 0.017 −0.318 −0.341 −0.108 0.064 −0.585 0.613 −0.077 CD97-Tango −0.649 −0.329 −0.606 −0.780 −0.899 −0.468 −0.788 −0.937 CHRM1-Tango −0.627 −0.307 −0.384 −1.367 −1.117 −1.050 −0.027 0.325 CHRM2-Tango −1.099 −1.089 −0.815 −1.094 −0.589 −1.318 −0.492 −0.513 CHRM3-Tango −1.005 −1.198 −0.938 −0.643 −0.778 −1.264 −0.630 0.277 CHRM4-Tango −0.453 −0.217 0.115 −0.031 0.377 −0.490 −0.130 0.284 CHRM5-Tango −0.571 −0.529 0.133 −0.377 0.260 −0.255 −0.660 0.576 CMKLR1-Tango −0.296 −0.154 −0.074 −0.227 −0.017 −0.517 −0.039 −0.296 CNR1-Tango −0.641 −0.770 −0.450 −0.546 −0.645 −0.469 −0.036 −0.458 CNR2-Tango −0.192 −0.412 −0.908 −0.537 −0.495 −0.500 −0.109 0.202 CRHR1-Tango 0.108 0.037 −0.458 0.117 −0.506 −0.127 −0.703 0.255 CRHR2-Tango −0.052 0.165 −0.404 −0.819 0.172 −1.396 −0.537 −0.405 CX3C1-Tango 0.150 0.087 −0.750 −0.721 −0.390 −0.674 −0.471 −0.034 CXCR1-Tango −0.114 0.023 −0.018 −0.441 0.537 −0.400 0.067 0.573 CXCR2-Tango −0.500 −0.628 −0.138 −0.110 0.373 0.291 −0.956 0.700 CXCR3-Tango −0.410 −0.289 −0.215 0.134 −0.240 −0.100 0.155 0.326 CXCR4-Tango −0.610 −0.353 0.066 0.011 0.445 −0.258 −0.320 0.549 CXCR5-Tango −0.309 −0.143 −0.431 −0.157 0.036 −0.103 −0.146 0.292 CXCR6-Tango −0.372 −0.147 0.022 −0.819 −0.642 −0.920 0.196 1.000 CXCR7-Tango −1.169 −0.784 −1.397 −0.796 −1.099 −0.389 −1.048 −0.280 CYSLTR1-Tango −0.759 −0.229 −0.629 −0.171 0.003 −0.030 −0.598 0.490 CYSLTR2-Tango −0.315 −0.493 −0.114 0.079 0.023 −0.698 0.159 0.592 DRD1-Tango −0.813 −0.997 −0.371 −0.361 −0.229 −0.384 −0.925 0.809 DRD2-Tango −0.448 −0.187 −0.080 −1.275 −0.671 −2.112 −1.538 1.093 DRD3-Tango −1.091 −0.948 −0.087 −2.246 0.160 −2.709 0.136 1.381 DRD4-Tango −0.690 −0.690 1.062 −1.597 0.000 −3.121 −1.322 1.140 DRD5-Tango −0.358 −0.232 −0.661 −0.646 −0.587 −0.770 −0.584 −0.079 EDNRA-Tango −0.369 −0.414 −0.436 −0.350 −0.243 −0.303 −0.541 0.441 EDNRB-Tango −0.457 −0.323 −0.765 −0.445 −0.604 −0.445 −0.208 −0.376 ELTD1-Tango −0.922 −0.692 −0.870 −0.632 −0.725 −0.423 −1.002 −0.800 F2R-Tango −0.230 −0.057 −0.319 −0.131 −0.069 −0.306 −0.202 −0.105 F2RL1-Tango −0.160 −0.058 0.482 −0.161 −0.154 −0.332 −0.199 −0.057 F2RL2-Tango −0.722 −0.353 −0.133 −0.585 0.166 −0.773 −0.252 −0.281 F2RL3-Tango −0.775 −0.280 −0.361 −0.141 −0.168 −0.621 −0.486 −0.061 FFA1-Tango −0.727 −0.576 −0.088 −0.150 0.053 0.012 −0.258 −0.339 FFA2-Tango −0.963 −0.578 −0.367 −0.407 −0.417 −0.432 −0.121 −0.319 FFA3-Tango −0.575 −0.346 0.061 −0.120 0.000 −0.535 −0.396 0.367 FPR1-Tango −0.966 −0.455 −1.343 −1.430 −1.362 −1.301 −1.657 −0.546 FPR2-Tango −0.299 −0.212 −0.599 −0.640 −0.468 −1.067 0.077 0.488 FPR3-Tango −0.670 −0.355 −0.493 −0.403 −0.245 −0.428 −0.365 0.010 FSHR-Tango −0.645 −0.596 −0.548 −0.470 −0.482 −0.736 −0.534 −0.352 GABBR1-Tango 0.000 −0.139 −0.384 −0.790 −0.384 −0.727 −0.178 0.038 GAL1-Tango −0.740 −0.740 −1.430 −0.748 −2.062 −0.595 −0.893 −1.222 GAL2-Tango −0.514 −0.147 −0.878 −0.707 −1.216 −1.209 −0.084 0.885 GAU-Tango −0.672 −0.126 −1.383 −1.209 −2.101 −1.472 −0.491 −0.209 GCGR-Tango −0.849 −0.651 −0.826 −0.533 −0.465 −0.678 −0.702 −0.573 GHRHR-Tango −0.248 −0.802 −0.357 −0.399 −0.435 −1.177 −0.786 −0.408 GHSR-Tango −0.705 −0.389 −0.177 −0.508 −0.152 −0.499 −1.030 −2.232 GIPR-Tango −0.769 −0.359 −0.020 −0.607 −0.405 −0.607 −0.732 −0.491 GLP1R-Tango −0.462 −0.029 −1.175 −0.991 −0.844 −1.564 −0.996 −0.024 GLP2R-Tango −0.120 −0.364 −0.768 0.184 −0.768 −0.112 −0.372 0.089 GNRHR-Tango −0.253 −0.183 −0.122 −0.745 −0.480 −0.872 0.089 −1.000 GPBA-Tango −0.379 −0.379 −1.272 −0.399 −0.735 −0.077 0.347 −1.063 GPER-Tango −0.076 −0.034 −0.164 −0.245 −1.189 −0.569 −0.282 0.075 GPR1-Tango −0.508 −0.567 −0.353 −1.025 −0.527 −1.170 −1.166 −0.787 GPR101-Tango −0.411 −0.387 −0.295 −0.603 −0.025 −0.672 −0.174 −0.536 GPR110-Tango −0.345 −0.493 −0.978 −0.807 −0.709 −0.996 −0.369 −0.043 GPR111-Tango −0.057 −0.509 −0.627 −0.968 −0.579 −1.422 −0.468 0.083 GPR113-Tango −0.521 −0.847 −0.666 −0.021 −0.700 −0.521 −0.781 −0.511 GPR114-Tango −0.260 −0.400 −0.778 −0.391 −0.182 −0.493 −0.438 −0.262 GPR115-Tango −0.917 −1.032 −0.785 −0.367 −0.318 −0.472 −0.725 −0.287 GPR116-Tango −0.424 −0.616 −0.886 −0.485 −0.499 −0.351 −0.594 −0.613 GPR119-Tango −0.339 −0.278 −0.351 −0.401 −0.509 −0.821 −0.213 −0.384 GPR12-Tango −0.326 −0.300 −0.135 −0.106 0.255 −0.305 −0.718 −0.119 GPR120-Tango 0.272 0.016 −0.242 −0.401 −0.065 −0.524 −0.326 −0.105 GPR123-Tango −0.291 −0.019 −0.192 −0.448 0.221 −0.696 −0.486 0.131 GPR124-Tango −0.627 −0.818 −0.926 −0.494 −0.620 −0.229 −0.043 0.223 GPR125-Tango −0.914 −0.799 −0.588 −0.305 0.160 −0.139 −0.362 0.551 GPR126-Tango −0.743 −0.681 −1.017 −0.749 −0.762 −0.727 −0.366 −0.200 GPR132-Tango −0.810 −0.824 −1.028 −0.545 −0.611 −0.995 −0.268 −0.014 GPR133-Tango −0.866 −0.876 −0.724 −0.406 −0.449 −0.379 −0.277 −0.237 GPR135-Tango −0.478 −0.562 −0.604 −0.325 −0.199 −0.376 −0.339 0.147 GPR141-Tango −0.514 −0.339 −0.267 −0.308 −0.127 −0.130 −0.422 0.335 GPR142-Tango −0.831 −0.820 −0.738 −0.011 −0.501 −0.061 −0.243 −0.331 GPR143-Tango −0.672 −0.585 −0.511 −0.395 −0.143 −1.230 −0.283 −0.087 GPR144-Tango −0.858 −0.545 −1.222 −0.929 −1.033 −1.000 −0.560 −0.918 GPR146-Tango −0.565 −0.395 0.170 −0.685 0.104 −0.525 0.000 −0.472 GPR148-Tango 0.181 0.775 −1.122 0.074 −0.402 −0.407 0.828 0.778 GPR149-Tango −0.522 −0.785 −0.250 −0.939 −0.310 −1.064 −1.006 −0.658 GPR15-Tango −0.543 −0.477 −0.554 −0.229 0.077 −0.500 −0.483 −0.462 GPR150-Tango −0.091 −0.335 −0.350 −0.068 0.160 0.127 0.040 −0.686 GPR151-Tango −0.820 −0.526 −0.330 −0.475 −0.284 −0.503 0.095 −0.435 GPR152-Tango −0.480 −0.545 −0.756 0.005 −0.886 −0.208 0.510 −0.165 GPR153-Tango −0.694 −0.595 −0.240 −0.516 0.052 −0.446 0.461 0.250 GPR156-Tango −0.578 −0.372 −0.793 −0.652 −0.800 −0.430 −0.101 −0.319 GPR157-Tango −0.598 −0.402 0.242 −0.239 −0.269 −0.603 0.263 −0.102 GPR158-Tango −0.114 −0.258 −0.994 −0.471 −0.978 −0.174 0.196 −0.113 GPR160-Tango −0.695 −0.460 −0.238 −0.046 −0.209 −0.281 0.674 0.009 GPR161-Tango −0.308 −0.413 0.108 −0.385 0.130 −0.283 0.409 0.135 GPR162-Tango −0.623 −0.678 −0.258 −0.421 −0.326 −0.688 −0.064 −0.196 GPR17-Tango −0.910 −1.103 0.093 0.044 0.151 0.197 −0.187 0.117 GPR171-Tango −0.555 −0.854 −0.117 −0.009 −0.117 −0.078 −0.040 −0.137 GPR173-Tango −0.705 −0.352 −0.081 −0.045 −0.293 0.153 −0.129 −0.361 GPR174-Tango −0.306 −0.068 −0.060 0.308 −0.157 −0.212 −0.180 0.474 GPR18-Tango −0.369 −0.120 −0.145 −0.338 −0.177 −0.331 −0.094 −0.165 GPR182-Tango −0.916 −0.949 0.141 −0.387 0.066 −0.259 −0.321 −0.157 GPR183-Tango −0.404 −0.369 −0.376 −0.060 −0.284 −0.186 −0.149 0.106 GPR19-Tango −0.814 −0.576 −0.087 −0.086 0.069 −0.207 −0.219 −0.020 GPR20-Tango −0.098 −0.241 −0.112 −0.585 0.511 −0.481 0.489 0.170 GPR21-Tango 0.000 −0.146 −0.563 −0.437 −0.719 −0.905 −0.080 0.030 GPR22-Tango 0.044 0.194 −0.013 −0.147 −0.169 −0.428 −0.115 0.349 GPR25-Tango 0.132 −0.019 −0.178 −0.226 −0.537 −0.589 −0.211 0.265 GPR26-Tango −0.535 −0.307 0.436 −0.280 0.700 −0.280 −0.195 −0.429 GPR27-Tango −0.063 −0.063 0.308 −0.302 −0.016 −0.511 0.104 0.969 GPR3-Tango −0.707 −1.002 −0.081 −0.125 −0.150 0.328 −0.084 0.657 GPR31-Tango −0.737 −0.713 0.142 −0.336 −0.072 −0.453 −0.314 0.447 GPR32-Tango −0.204 −0.183 −0.103 −0.646 −0.255 −0.426 −0.527 −0.491 GPR34-Tango −0.431 −0.261 −1.090 −1.374 −0.628 −1.040 −0.704 −0.264 GPR35-Tango −0.663 −0.588 −0.572 −0.461 −0.167 −0.633 −0.525 −0.374 GPR37-Tango −0.289 −0.012 −0.040 −0.924 0.362 −0.899 −0.518 0.260 GPR37L1-Tango −0.327 −0.481 −0.382 −0.212 0.304 −0.397 −0.562 −0.292 GPR39-Tango −0.564 −0.344 −0.612 −0.390 −0.604 −0.414 −0.346 −0.446 GPR4-Tango −0.449 −0.572 −0.299 −0.758 0.264 −0.711 −0.669 0.471 GPR44-Tango −0.456 −0.545 −0.503 −0.520 −0.176 −0.964 −0.610 −0.014 GPR45-Tango −0.229 −0.387 −0.252 −0.403 −0.293 −0.525 −1.054 −0.824 GPR50-Tango 0.089 0.451 0.115 0.158 0.677 −0.206 −0.101 0.896 GPR52-Tango −0.089 0.081 0.289 −0.074 0.424 −0.713 −0.530 0.635 GPR55-Tango −0.266 −0.016 −0.046 −0.970 0.311 −0.782 −0.440 −0.090 GPR56-Tango 0.839 1.109 1.301 −0.628 1.224 −0.403 −0.475 0.217 GPR6-Tango −0.047 −0.047 0.294 −0.321 0.503 −0.261 −0.348 0.240 GPR61-Tango −0.408 0.315 0.088 −0.160 0.604 −0.640 −0.289 0.439 GPR62-Tango 0.048 −0.191 −0.200 −0.184 0.000 −0.292 −0.025 0.058 GPR63-Tango −0.612 −0.954 −0.790 −0.453 −0.358 −0.715 −0.452 −0.212 GPR64-Tango −0.544 −0.641 −0.629 −0.850 −0.538 −0.682 −0.775 −0.098 GPR65-Tango −0.148 −0.344 −0.446 −0.494 −0.026 −0.713 −0.307 0.322 GPR68-Tango −0.401 −0.441 −0.606 −0.764 −0.005 −1.123 −0.839 0.212 GPR75-Tango 0.242 0.526 0.016 −0.144 0.243 −0.733 −0.464 0.480 GPR77-Tango −0.162 0.039 −0.312 −0.754 −0.249 −1.176 −0.980 −1.180 GPR78-Tango 0.074 0.347 0.448 0.069 0.635 −0.481 −0.025 0.279 GPR82-Tango −0.534 0.028 −0.063 −0.255 0.015 −0.749 −0.755 0.092 GPR83-Tango −0.017 0.084 −0.382 −0.290 −0.095 −0.695 −0.372 0.114 GPR84-Tango −0.300 −0.231 −0.421 −0.369 0.225 −0.730 −0.573 0.305 GPR85-Tango 0.154 0.089 −0.610 −0.172 −0.298 −0.464 −0.265 1.017 GPR87-Tango −0.188 0.077 −0.140 −0.203 0.395 −0.312 −0.368 0.432 GPR88-Tango −0.007 0.104 −0.300 −0.490 0.089 −0.781 −0.038 0.411 GPR97-Tango −0.097 0.212 −0.295 −0.445 −0.165 −0.787 −0.578 −0.270 GPRC5A-Tango −0.174 0.153 −0.088 −0.339 0.145 −0.404 −0.581 −0.395 GPRC5B-Tango −0.271 0.272 −0.415 −0.293 −0.029 −0.467 −0.452 0.284 GPRC5C-Tango 0.410 0.241 −0.358 −0.023 0.048 −0.258 −0.378 0.473 GPRC5D-Tango 0.017 0.021 −0.807 −0.617 −0.460 −0.709 −0.429 −0.160 GPRC6A-Tango −0.002 −0.090 −1.281 −0.491 −0.790 −0.628 −0.648 0.205 GRM1-Tango 0.032 −0.309 −1.198 −0.153 −0.747 −0.178 −0.789 0.214 GRM2-Tango −0.350 −0.170 −0.856 −0.760 −0.688 −0.880 −0.709 −0.305 GRM4-Tango 0.114 0.119 −0.399 −0.698 −0.165 −1.096 −0.379 0.367 GRM5-Tango −0.403 −0.209 −1.166 −0.984 −0.378 −1.233 −0.309 0.236 GRM6-Tango −0.210 −0.330 −0.745 −0.850 −0.184 −0.877 −0.773 0.256 GRM7-Tango −0.190 −0.161 −1.645 −0.848 −1.463 −1.094 −1.253 −0.318 GRM8-Tango −0.539 −0.350 −0.829 −0.806 −0.361 −0.922 −0.531 −0.294 GRPR-Tango 0.335 0.086 0.014 0.074 −0.199 −0.615 −0.389 0.000 HCA1-Tango 0.163 −0.124 0.144 0.457 −0.230 −0.257 −0.481 −0.077 HCA2-Tango 0.241 0.272 −0.217 −0.356 0.028 −0.428 −0.433 0.246 HCA3-Tango −0.328 −0.440 −0.382 0.471 −0.755 0.077 −0.193 0.065 HCTR1-Tango −0.521 −0.111 −0.078 0.122 −0.623 0.000 −0.261 0.087 HCTR2-Tango −0.549 0.106 −0.686 −1.234 −0.255 −0.112 0.060 −1.280 HRH1-Tango −0.774 −0.605 −0.098 −0.473 0.329 −0.212 −0.203 0.174 HRH2-Tango −0.569 −0.253 −0.068 −0.069 −0.022 −0.377 0.132 0.511 HRH3-Tango −0.269 0.516 −0.442 −0.515 −0.456 0.454 −0.068 0.634 HRH4-Tango −0.075 0.228 −0.231 −0.310 0.160 −0.037 −0.144 0.716 HTR1A-Tango 0.080 −0.007 0.143 −0.215 0.587 −0.374 −0.078 0.736 HTR1B-Tango −0.079 −0.273 −0.111 −0.636 0.446 −0.618 −0.173 0.686 HTR1D-Tango −0.210 −0.476 −0.182 −0.374 0.168 −0.312 0.000 0.404 HTR1E-Tango −0.509 −0.293 0.189 −0.223 0.789 −0.297 −0.097 0.347 HTR1F-Tango −0.237 −0.224 0.182 −0.434 0.451 −0.454 −0.699 0.838 HTR2A-Tango −0.066 −0.277 −0.066 −0.747 0.148 −0.471 −0.534 0.276 HTR2B-Tango −0.210 −0.491 −0.585 −0.911 −0.317 −0.915 −0.598 0.607 HTR2C-Tango −0.032 −0.320 −0.101 −0.595 −0.259 −0.827 −0.360 −0.187 HTR4-Tango −0.264 −0.342 −0.208 −0.197 0.243 −0.795 −0.592 0.029 HTR5-Tango −0.443 0.035 0.373 0.209 0.504 −0.241 −0.566 0.768 HTR6-Tango 0.431 0.274 −0.259 −0.455 −0.136 −0.573 0.082 0.200 HTR7-Tango 0.275 0.743 0.041 −0.785 0.397 −0.605 −0.281 0.487 KISSPEPTIN-R- −0.267 0.477 0.204 0.127 0.000 −0.531 0.073 0.000 Tango LHCGR-Tango −0.231 −0.055 −0.716 −0.089 −0.422 −0.142 −0.541 −0.300 LPAR1-Tango −0.290 0.364 −0.352 −0.430 0.108 −0.529 −0.448 −0.155 LPAR2-Tango −0.384 −0.045 −0.540 −0.343 −0.421 −0.814 −0.138 −0.582 LPAR4-Tango −0.275 −0.161 −0.154 −0.670 −0.012 −1.036 −0.529 −0.477 LPAR5-Tango −0.287 −0.334 −0.439 −0.642 −0.017 −0.702 −0.542 −0.934 LPAR6-Tango −0.146 0.408 −0.132 −0.095 0.544 −0.251 −0.394 0.543 LTB4R-Tango −0.175 0.076 0.165 −0.153 0.537 −0.117 −0.178 −0.027 LTB4R2B-Tango −0.142 0.082 −0.407 0.115 0.188 −0.373 −0.450 0.129 MAS1-Tango −0.074 −0.162 −0.327 −0.338 0.006 −0.294 −0.582 0.256 MAS1L-Tango −0.213 −0.081 −0.303 −0.644 0.005 −0.721 −0.497 −0.459 MC1R-Tango 0.096 −0.079 −0.204 −0.354 0.278 −0.605 −0.127 −0.446 MC2R-Tango 0.107 0.171 −0.055 −0.482 −0.055 −0.317 −0.495 0.426 MC3R-Tango 0.239 0.995 0.364 0.132 0.267 −1.216 −0.720 0.560 MC4R-Tango 0.166 0.953 −0.100 −0.122 −0.429 −0.032 −0.467 0.395 MC5R-Tango 0.531 1.081 0.344 0.339 0.314 −0.585 −0.441 0.344 MCHR1-Tango −1.287 −0.819 −0.232 −0.177 −0.417 −0.368 −0.996 −0.599 MCHR2-Tango −0.430 −0.170 −0.018 −0.835 −0.129 −0.568 −0.312 −0.296 MLNR-Tango −0.134 −0.391 −0.363 −0.381 0.116 −1.064 0.280 0.244 MRGPRD-Tango −0.696 −0.723 −0.011 −0.357 0.357 −0.285 −0.438 −0.157 MRGPRE-Tango −0.209 −0.143 −0.604 −0.484 −0.329 −0.588 −0.238 −0.039 MRGPRF-Tango −0.113 0.008 −0.055 −0.520 0.461 −0.608 −0.779 0.038 MRGPRG-Tango −0.622 −0.815 −0.448 −0.793 0.199 −0.636 −0.831 0.027 MRGPRX1-Tango −0.766 −0.905 −0.334 −0.067 −0.029 −0.236 −0.807 0.013 MRGPRX2-Tango −0.220 −0.554 −0.185 −1.030 −0.160 −1.112 −0.832 −0.262 MRGPRX3-Tango −0.327 −0.420 −0.417 −0.485 −0.487 −1.340 −0.482 −0.141 MRGPRX4-Tango −0.882 −0.361 −1.011 −1.332 −0.372 −1.673 −1.183 −0.815 MTNR1A-Tango −0.126 0.126 −0.050 −0.784 −0.172 −0.700 −0.956 −0.109 MTNR1B-Tango −0.058 −0.352 0.117 −0.334 0.032 −0.443 −0.762 −0.273 NMBR-Tango 0.161 0.316 0.137 0.009 −0.182 −0.891 −0.594 0.119 NMUR1-Tango −0.564 −0.234 −0.545 0.328 −0.545 −0.315 −0.762 −0.406 NMUR2-Tango 0.357 0.290 −0.569 −0.290 −0.642 −0.097 −0.833 −0.363 NPBW1-Tango −0.463 −0.108 0.523 −0.955 0.480 −1.406 0.368 0.631 NPBW2-Tango −0.672 −0.195 −0.560 −1.247 −0.163 −1.266 −0.550 −0.323 NPFF1-Tango 0.220 −0.052 −0.128 −0.675 −0.267 −1.212 −0.331 −0.458 NPFF2-Tango 0.139 0.575 −0.388 0.171 −0.173 −0.237 −0.329 −0.776 NPS-Tango 0.005 0.163 −0.865 0.280 −0.030 −0.519 −0.489 −0.317 NPY1R-Tango 0.005 0.421 −0.143 −0.050 0.259 −0.876 −0.403 −1.168 NPY2R-Tango 0.014 0.416 −0.815 −0.822 0.104 −1.578 −0.336 −0.020 NPY4R-Tango −0.540 −0.126 −0.805 −0.628 −0.504 −1.350 −0.444 −0.148 NPY5R-Tango −0.213 −0.168 −0.710 −0.468 −0.344 −1.293 −0.452 −0.249 NTSR1-Tango −0.365 0.108 −1.005 −1.108 −0.544 −1.637 −0.715 −0.506 NTSR2-Tango 0.125 0.125 −0.561 −0.119 −0.229 −0.720 −0.544 −0.386 OPN3-Tango 0.000 −0.077 0.537 −0.311 0.165 −0.896 −0.155 0.027 OPN5-Tango −0.643 −0.748 −0.600 −0.863 −0.056 −0.966 −1.033 −0.608 OPRD1-Tango −0.325 −0.551 −0.749 −0.440 −0.595 −0.715 −0.586 −0.018 OPRK1-Tango 0.146 0.401 −0.113 −0.307 0.269 −0.277 0.160 0.294 OPRL1-Tango −0.206 −0.159 −0.202 −0.831 −0.092 −1.143 −0.205 0.585 OPRM1-Tango 0.242 0.463 −0.484 −0.493 −0.553 −0.084 −0.503 −1.406 OXER1-Tango −0.380 0.203 −0.322 −0.835 0.066 −0.683 −0.804 −0.288 OXGR1-Tango −0.587 −0.201 −0.480 −0.204 −0.343 −0.548 −0.759 −0.943 OXTR-Tango −0.036 0.348 −0.352 −0.509 −0.481 −0.848 −0.550 −0.085 P2RY1-Tango −0.410 −0.333 −0.844 −0.971 −0.793 −0.756 −0.177 −0.004 P2RY10-Tango −0.017 0.020 −0.805 −0.945 −0.832 −0.552 −0.216 −0.200 P2RY11-Tango −0.564 −0.286 −1.324 −0.585 −0.859 −0.852 −0.449 −0.284 P2RY12-Tango −0.246 0.121 −0.292 −1.455 −0.620 −1.290 −1.377 −0.199 P2RY13-Tango −0.576 −0.744 −0.389 −0.261 −0.169 −0.310 −0.336 −0.237 P2RY14-Tango −1.017 −0.653 −0.505 −0.996 −0.167 −0.852 −0.238 −0.273 P2RY2-Tango −0.910 −0.944 −0.784 −1.056 −0.617 −1.099 −0.517 −0.305 P2RY4-Tango −1.134 −1.261 −0.666 −1.008 −0.481 −0.957 −0.707 0.011 P2RY6-Tango −0.868 −1.128 −0.831 −0.952 −0.415 −1.037 −0.210 0.288 P2RY8-Tango −1.023 −1.245 −0.775 −0.372 −0.500 −0.849 −0.700 −0.026 PK1-Tango −0.627 −0.720 −0.110 −0.893 −0.300 −0.475 −0.220 0.036 PK2-Tango −0.377 −0.467 −1.317 −0.227 −0.585 −0.435 −0.013 0.355 PRRP-Tango −0.465 −0.472 −0.337 −1.183 −0.450 −0.832 −0.106 0.050 PTAFR-Tango −0.685 −0.468 −0.687 −1.056 −0.446 −0.850 −0.411 0.579 PTGDR-Tango −0.426 −0.534 −0.443 −0.780 −0.317 −0.496 −0.408 −0.185 PTGER1-Tango −0.023 0.188 0.292 −0.600 0.037 −0.366 −0.071 −0.420 PTGER2-Tango 0.183 −0.339 −0.463 0.165 −0.399 0.000 −0.146 0.397 PTGER3-Tango −0.589 −0.672 −0.419 −0.267 −0.397 −0.225 −0.612 0.134 PTGER4-Tango 0.250 0.667 0.339 −0.128 0.517 −0.019 −0.240 0.696 PTGFR-Tango −0.316 −0.036 −0.200 0.077 0.098 −0.923 −0.512 0.260 PTGIR-Tango −0.627 −1.093 −0.437 −0.073 −0.553 −0.423 0.045 1.148 PTH1R-Tango −0.091 −0.286 −0.931 −0.814 −0.301 −0.535 −0.436 0.477 PTH2R-Tango −1.150 −1.345 −1.214 −1.006 −0.738 −0.443 −0.921 0.606 QRFP-Tango −0.084 −0.350 −0.780 −0.627 −1.070 −0.647 −0.410 0.243 RXFP1-Tango −0.764 −1.139 −0.606 0.085 −0.408 −0.430 −0.871 −0.419 RXFP2-Tango −1.158 −1.117 −1.071 −0.587 −0.824 −0.857 −0.813 0.123 RXFP3-Tango −0.323 −0.167 −0.750 −0.277 −1.229 −0.556 −1.168 −0.483 RXFP4-Tango −0.790 −1.025 −0.888 −0.054 −1.122 −0.054 −0.907 −0.062 S1PR1-Tango −0.580 −0.410 −0.428 0.539 0.420 0.564 −0.439 −0.458 S1PR2-Tango −0.741 −0.391 −0.362 0.144 0.208 −0.144 −0.378 −0.183 S1PR3-Tango 0.215 −0.505 −0.240 0.465 0.100 0.000 −0.448 0.230 S1PR4-Tango −0.402 0.366 −0.234 −0.482 0.285 0.572 −0.869 −0.856 S1PR5-Tango −0.125 0.132 −0.523 0.000 0.000 0.336 −0.394 0.721 SCTR-Tango 0.077 0.263 0.284 −0.200 0.210 0.060 −0.009 0.766 SSTR1-Tango 0.000 0.189 0.364 −0.260 0.603 −0.052 −0.476 −0.538 SSTR2-Tango 0.547 0.493 0.091 0.078 0.883 −0.329 −0.593 0.179 SSTR3-Tango 0.272 0.077 0.564 0.497 0.411 −0.415 −0.316 1.148 SSTR4-Tango 0.164 0.000 0.729 0.255 0.940 −0.050 −0.378 0.913 SSTR5-Tango 0.733 0.489 2.043 0.807 2.316 0.198 0.651 0.684 SUCNR1-Tango 2.558 2.703 3.885 3.097 4.249 3.176 3.289 2.569 TA1-Tango −0.340 0.101 0.085 0.118 0.427 −0.595 −0.275 1.193 TAAR2-Tango 0.065 −0.076 −0.345 0.307 0.050 0.132 −0.420 0.143 TAAR5-Tango −0.552 −0.539 −0.562 −0.131 0.009 −0.230 −0.284 0.079 TAAR6-Tango −0.875 −0.770 −0.484 −0.607 0.373 −0.816 −0.759 −0.347 TAAR8-Tango −0.608 −0.750 −0.987 0.090 −0.335 −0.413 −0.556 −0.300 TAAR9-Tango −0.518 −0.716 −0.632 −0.315 −0.428 −0.422 −0.554 −0.581 TACR1-Tango −0.693 −0.624 −0.496 −0.621 −0.149 −0.933 −0.640 0.079 TACR2-Tango −0.503 −0.604 −0.233 −0.132 −0.063 −0.444 −0.368 0.050 TACR3-Tango −0.374 −0.652 −0.308 −0.348 −0.049 −0.730 −0.697 −0.126 TBXA2R-Tango −0.409 −0.693 −0.423 −0.503 −0.280 −0.788 −0.428 −0.392 TSHR-Tango −0.842 −1.096 −0.529 −0.491 −0.020 −0.816 −0.539 −0.192 UTS2R-Tango −0.086 −0.199 −0.050 0.251 0.260 −0.094 0.029 −1.097 VIPR1-Tango −0.256 −0.083 −0.148 −0.673 −0.089 −0.759 −0.924 −0.993 VIPR2-Tango −0.023 0.725 1.685 −0.387 1.578 −0.260 0.240 1.255 PRESTO-Tango Parabacteroides Fusobacterium Fusobacterium Collinsella Collinsella Collinsella Collinsella Collinsella with GMM distasonis-2# spp-1# spp-2# aerofaciens-1# aerofaciens-2# aerofaciens-3# stercoris-1# stercoris-2# ADCYAP1R1- −0.731 −0.891 −1.163 −0.043 −0.262 0.024 0.105 −0.159 Tango ADORA1-Tango −0.779 −1.830 −1.785 −1.003 −0.847 −0.492 −0.515 −0.310 ADORA2A-Tango −0.083 −0.711 −0.260 −0.227 −0.461 0.234 −0.440 −0.425 ADORA2B-Tango −0.437 −0.443 −0.706 −0.269 −0.432 0.011 −0.210 −0.433 ADORA3-Tango −0.260 −0.615 −0.703 0.240 0.035 0.443 −0.177 0.110 ADRA1A-Tango −0.111 −0.532 −0.786 −0.134 −0.474 −0.071 −0.133 −0.328 ADRA1B-Tango −0.002 −0.734 −0.881 0.154 0.181 0.252 −0.380 0.115 ADRA1D-Tango −0.427 −0.921 −0.699 −0.089 −0.363 −0.204 −0.470 0.180 ADRA2A-Tango −0.086 −0.346 −0.803 −0.175 −0.367 0.612 −0.412 −0.473 ADRA2B-Tango −1.200 −1.807 −0.993 −0.903 −0.720 −0.144 −0.750 −0.157 ADRA2C-Tango 0.626 −0.462 −0.267 −0.882 −0.197 0.908 −0.158 −0.030 ADRB1-Tango −0.886 −0.463 −1.303 0.032 −0.160 0.727 0.760 0.288 ADRB2-Tango −0.774 −0.993 −1.068 0.265 0.126 0.645 0.124 −0.086 ADRB3-Tango 0.352 −0.434 −0.148 0.350 0.507 −0.127 0.156 −0.104 AGTR1-Tango 0.419 −0.968 −0.934 0.011 −0.016 0.088 0.073 −0.154 AGTR2-Tango −0.069 −1.347 −1.307 −0.217 −0.233 0.049 0.325 0.190 APJ-Tango −0.455 −1.765 −1.070 −0.624 −0.716 0.437 −0.143 0.365 AVPR1A-Tango −0.390 −1.118 −0.955 0.401 0.348 0.459 −0.322 0.097 AVPR1B-Tango 0.025 −1.213 −1.214 0.216 0.051 0.233 −0.082 0.031 AVPR2-Tango −0.337 −1.248 −1.524 −0.396 −0.366 0.254 −0.050 0.234 BB3-Tango −0.689 −1.328 −1.706 −0.370 −0.447 −0.246 0.129 −0.065 BDKBR1-Tango 0.117 −0.473 −0.764 −0.313 −0.228 0.426 0.241 0.307 BDKBR2-Tango 0.150 −1.147 −1.200 0.193 0.287 0.434 0.399 0.266 C3AR1-Tango −0.665 −0.992 −1.255 −0.101 −0.032 0.060 −0.192 0.000 C5A-Tango 0.023 −0.887 −0.719 0.483 0.407 0.435 0.198 0.047 CALCRb-Tango −0.242 −0.056 −0.548 0.287 0.024 0.200 −0.021 −0.010 CALCRL-Tango 0.057 −0.147 −0.244 −0.144 −0.040 −0.112 −0.205 −0.373 CASR-Tango −0.046 −1.160 −1.011 0.181 0.385 0.276 0.081 0.330 CCKAR-Tango 0.059 −1.068 −0.878 0.323 0.274 0.129 0.058 0.305 CCKBR-Tango −0.344 −0.902 −0.725 −0.382 −0.218 0.231 −0.014 0.234 CCR10-Tango −0.363 −0.705 −0.938 0.048 0.138 0.234 −0.130 0.183 CCR2-Tango −0.555 −0.903 −0.666 −0.336 −0.147 0.562 0.067 0.413 CCR3-Tango −0.753 −1.669 −1.862 −0.426 −0.526 −0.268 −0.202 −0.091 CCR4-Tango −0.589 −1.184 −0.915 0.742 0.556 0.592 −0.074 0.310 CCR5-Tango 0.000 −0.948 −0.889 0.507 0.751 0.252 0.075 0.159 CCR6-Tango 0.000 −0.962 −1.307 0.758 0.657 0.531 0.057 0.435 CCR7-Tango −0.808 −1.187 −0.766 0.537 0.333 0.120 0.026 0.035 CCR8-Tango −0.818 −1.076 −0.585 −0.187 −0.320 0.623 −0.204 0.313 CCRL2-Tango −0.718 −0.932 −0.415 0.285 0.354 0.291 −0.468 −0.468 CD97-Tango −0.053 −1.285 −1.437 −1.234 −0.671 0.184 −0.140 −0.054 CHRM1-Tango 0.422 −0.198 −0.260 −0.184 −0.754 0.252 0.000 −0.337 CHRM2-Tango 0.471 −1.398 −1.469 −0.650 −0.403 0.259 0.196 0.262 CHRM3-Tango −0.041 −0.415 −0.807 −0.497 −0.628 0.343 0.021 0.121 CHRM4-Tango 0.171 −0.274 −0.442 0.151 0.327 0.208 0.034 0.067 CHRM5-Tango 0.092 −1.193 −1.101 0.220 0.355 0.112 0.458 0.196 CMKLR1-Tango 0.004 −0.677 −0.778 0.216 0.255 0.302 0.050 0.134 CNR1-Tango 0.064 −1.211 −0.742 0.112 0.148 0.392 −0.023 0.406 CNR2-Tango 0.000 −0.923 −1.101 −0.319 −0.300 0.126 −0.158 0.196 CRHR1-Tango −0.292 −1.079 −0.722 −0.146 −0.411 0.260 0.068 −0.234 CRHR2-Tango −0.112 −0.826 −0.845 0.039 −0.218 0.367 −0.069 0.217 CX3C1-Tango −0.444 −0.451 −0.520 −0.230 −0.451 0.552 0.220 −0.118 CXCR1-Tango −0.033 −1.210 0.094 0.479 0.418 0.705 0.109 0.024 CXCR2-Tango 0.028 −0.481 −0.343 −0.365 −0.419 −0.072 0.661 0.202 CXCR3-Tango −0.016 −0.994 −0.554 −0.348 −0.590 0.375 0.214 0.178 CXCR4-Tango 0.266 −0.949 −1.401 0.003 −0.135 −0.100 0.420 0.082 CXCR5-Tango 0.163 −0.198 −0.604 −0.121 −0.282 0.117 0.197 0.099 CXCR6-Tango 0.028 −0.705 −0.769 0.524 0.340 0.595 0.239 0.227 CXCR7-Tango −0.412 −1.854 −1.919 −0.746 −0.709 0.122 0.277 −0.028 CYSLTR1-Tango −0.006 −1.508 −0.679 −0.231 −0.288 −0.049 0.236 0.054 CYSLTR2-Tango 0.088 −0.815 −0.140 0.245 0.225 0.489 0.299 0.108 DRD1-Tango −0.562 −1.904 −1.199 0.231 0.271 0.866 −0.383 0.008 DRD2-Tango −0.882 −1.117 −2.062 −0.223 0.000 1.027 −0.140 0.060 DRD3-Tango 0.190 −0.918 −0.737 0.477 0.415 2.232 −0.068 −0.386 DRD4-Tango −0.070 −0.745 −1.322 0.867 1.122 2.078 0.290 −0.108 DRD5-Tango −0.666 −1.067 −0.388 −0.241 −0.347 −0.148 −0.315 −0.357 EDNRA-Tango −0.208 −1.255 −0.688 0.158 0.219 0.325 0.186 0.509 EDNRB-Tango 0.119 −0.366 −0.263 −0.245 −0.245 0.148 0.229 −0.206 ELTD1-Tango −0.658 −1.939 −1.141 −0.709 −0.555 −0.379 −0.055 0.013 F2R-Tango 0.054 −1.532 −0.859 0.389 0.575 0.319 −0.134 0.211 F2RL1-Tango 0.276 −1.596 −0.784 0.356 0.621 0.202 0.065 0.366 F2RL2-Tango 0.036 −2.049 −1.239 0.392 0.440 0.228 −0.024 0.278 F2RL3-Tango −0.393 −1.311 −1.161 0.139 0.286 0.280 0.077 0.476 FFA1-Tango 0.125 −1.446 −0.904 −0.168 −0.211 0.086 0.397 0.225 FFA2-Tango 0.166 −0.890 −0.615 −0.167 −0.083 0.249 −0.002 0.045 FFA3-Tango 0.242 −1.556 −0.809 0.796 0.735 0.537 0.076 0.336 FPR1-Tango 0.508 −1.664 −0.882 1.101 1.004 1.038 0.788 −0.064 FPR2-Tango 0.558 −0.879 −0.079 0.628 0.494 0.941 0.201 0.292 FPR3-Tango 0.170 −1.603 −1.043 −0.289 −0.249 0.014 0.161 0.255 FSHR-Tango −0.199 −1.615 −0.941 −0.196 −0.189 −0.039 −0.150 −0.066 GABBR1-Tango 0.124 −0.364 −0.140 −0.091 −0.125 0.455 −0.038 −0.276 GAL1-Tango −1.074 −1.293 −0.611 −1.174 −1.080 0.252 0.112 −0.373 GAL2-Tango −0.258 −1.737 −1.627 −0.094 −0.216 0.698 −0.335 0.026 GAL3-Tango −0.391 −0.827 −0.680 −0.197 −0.235 0.889 0.133 0.435 GCGR-Tango −0.618 −0.909 −0.493 0.072 −1.076 0.314 −0.655 −0.206 GHRHR-Tango −0.442 −0.780 −0.942 0.100 −0.180 0.494 0.365 0.177 GHSR-Tango −1.166 −1.640 −1.785 −0.101 0.030 0.114 −0.308 0.085 GIPR-Tango −0.834 −1.149 −1.132 0.415 0.430 −0.247 −0.850 −0.284 GLP1R-Tango −1.171 −1.572 −1.557 −0.014 0.189 0.416 −0.596 0.464 GLP2R-Tango −0.148 −1.191 −1.326 −0.179 −0.187 0.047 −0.289 0.088 GNRHR-Tango −0.379 −0.626 −0.285 0.043 0.229 0.038 −0.039 0.264 GPBA-Tango −0.081 −0.453 −0.690 −0.401 −0.607 0.432 −0.142 0.367 GPER-Tango −0.660 −0.758 −0.731 −0.393 −0.092 0.317 0.064 0.037 GPR1-Tango −0.702 −1.218 −0.853 −0.126 −0.072 0.004 0.033 0.369 GPR101-Tango 0.246 −0.970 −0.448 −0.439 −0.186 0.125 0.348 0.261 GPR110-Tango −0.274 −1.376 −1.679 −0.395 −0.169 0.154 −0.444 −0.079 GPR111-Tango −0.077 −0.781 −0.696 −0.550 −0.135 0.372 0.102 −0.087 GPR113-Tango −0.781 −1.037 −0.481 0.000 0.165 0.145 −0.383 −0.177 GPR114-Tango −0.266 −0.147 0.066 −0.010 −0.102 −0.099 −0.126 −0.306 GPR115-Tango 0.159 −0.930 −0.870 −0.226 −0.294 −0.191 −0.271 −0.136 GPR116-Tango −0.421 −0.656 −1.018 −0.640 −0.315 −0.256 −0.566 −0.586 GPR119-Tango 0.040 −0.347 −0.538 −0.060 −0.203 0.187 −0.097 −0.377 GPR12-Tango −0.562 −1.160 −1.314 −0.373 −0.166 0.361 0.268 0.473 GPR120-Tango 0.078 −0.072 −0.575 0.268 0.288 0.040 0.167 −0.109 GPR123-Tango −0.072 −1.045 −0.817 0.078 0.310 0.227 −0.291 0.050 GPR124-Tango 0.131 −0.649 −1.454 −0.170 −0.271 −0.106 −0.426 −0.371 GPR125-Tango 0.046 −0.921 −1.519 −0.171 −0.065 −0.484 −0.349 −0.482 GPR126-Tango −0.002 −0.878 −1.169 −0.318 −0.372 −0.143 −0.395 −0.210 GPR132-Tango −0.344 −1.343 −1.170 −0.380 −0.321 0.031 −0.647 0.094 GPR133-Tango 0.332 −1.308 −1.755 −0.367 −0.304 −0.337 −0.430 −0.353 GPR135-Tango −0.021 −0.866 −0.836 −0.168 −0.044 0.104 −0.038 −0.336 GPR141-Tango −0.269 −0.397 −1.515 −0.204 −0.596 0.652 0.526 0.449 GPR142-Tango −0.382 −0.913 −1.745 −0.174 0.067 0.512 −0.604 0.308 GPR143-Tango −0.530 −1.717 −0.850 0.444 0.569 0.729 −0.168 0.722 GPR144-Tango −0.298 −1.443 −1.142 −0.596 −0.501 0.192 0.000 0.087 GPR146-Tango −0.369 −0.982 −0.554 0.632 0.350 0.260 0.435 0.208 GPR148-Tango 0.700 −0.503 −1.904 −0.122 −1.122 0.000 −0.087 0.160 GPR149-Tango −0.051 −1.452 −1.374 0.000 0.541 0.262 0.193 −0.180 GPR15-Tango −0.700 −1.449 −0.705 0.206 0.458 0.377 −0.394 0.829 GPR150-Tango 0.140 −0.251 −0.420 0.415 0.282 0.752 0.251 0.156 GPR151-Tango 0.421 −1.532 −0.781 −0.128 0.118 0.755 0.404 0.816 GPR152-Tango −0.005 −0.603 −0.994 −0.004 −0.204 0.139 0.149 0.109 GPR153-Tango 0.133 −1.374 −1.290 0.293 0.250 0.042 0.275 0.113 GPR156-Tango 0.104 −0.228 −1.223 −0.789 −0.779 −0.126 −0.113 −0.486 GPR157-Tango 0.202 −0.287 −0.913 0.200 0.129 0.588 0.255 0.034 GPR158-Tango −0.093 −0.624 −0.844 −0.418 −0.023 0.115 −0.198 −0.364 GPR160-Tango 0.075 −0.532 −0.557 0.527 0.513 0.506 0.236 0.358 GPR161-Tango 0.134 −0.978 −1.103 0.337 0.398 0.148 0.402 0.142 GPR162-Tango −0.281 −0.575 −0.704 0.006 0.014 −0.089 −0.030 −0.219 GPR17-Tango 0.407 −0.929 −0.885 0.154 0.089 0.064 0.588 0.307 GPR171-Tango 0.247 −0.200 −0.436 0.063 0.214 0.521 0.312 0.392 GPR173-Tango 0.078 −0.476 −0.651 0.326 0.084 0.346 0.166 0.027 GPR174-Tango −0.006 −0.738 −0.705 −0.032 0.198 0.049 0.275 0.082 GPR18-Tango −0.143 −1.021 −0.672 0.125 −0.023 0.181 0.489 0.330 GPR182-Tango 0.002 −1.222 −1.007 0.076 0.190 −0.334 0.149 0.060 GPR183-Tango −0.184 −0.791 −0.646 0.069 0.020 0.394 0.311 0.130 GPR19-Tango −0.221 −1.096 −1.087 0.440 0.448 0.142 0.074 0.097 GPR20-Tango 0.285 −1.122 −0.312 0.350 0.433 0.222 0.186 0.082 GPR21-Tango 0.036 −0.670 −0.712 −0.267 −0.128 0.324 0.076 −0.185 GPR22-Tango 0.145 −0.953 −1.280 0.294 0.358 0.260 0.157 0.173 GPR25-Tango −0.077 −0.805 −0.473 −0.129 −0.335 0.700 0.666 0.551 GPR26-Tango −0.419 −1.225 −1.310 0.564 0.322 0.131 0.111 0.657 GPR27-Tango 0.192 0.143 −0.365 −0.160 0.070 0.408 0.677 0.512 GPR3-Tango 0.121 −1.521 −1.314 −0.271 −0.372 0.142 0.972 0.428 GPR31-Tango −0.187 −1.444 −0.993 −0.228 −0.038 0.151 0.190 0.319 GPR32-Tango −0.471 −0.508 −0.628 −0.160 −0.691 0.057 −0.578 −0.110 GPR34-Tango −0.246 −0.903 −0.798 −0.489 −0.735 0.134 0.085 0.245 GPR35-Tango −0.291 −1.580 −1.554 −0.366 −0.228 0.284 0.374 0.218 GPR37-Tango 0.067 −1.419 −1.506 −0.129 −0.200 0.265 0.013 0.598 GPR37L1-Tango −0.163 −0.629 −1.228 0.038 0.162 0.015 0.209 0.246 GPR39-Tango −0.375 −0.766 −0.744 −0.483 −0.328 0.237 0.694 0.407 GPR4-Tango −0.176 −1.198 −1.181 0.153 0.095 0.353 0.359 0.487 GPR44-Tango −0.383 −0.489 −0.899 −0.277 −0.270 0.093 0.168 −0.069 GPR45-Tango −1.020 −1.170 −0.463 −0.146 0.057 −0.131 −0.216 −0.216 GPR50-Tango 0.129 −0.619 −0.435 0.717 0.927 0.466 0.231 0.193 GPR52-Tango 0.119 −0.856 −0.856 −0.062 0.227 0.457 0.665 0.651 GPR55-Tango −0.152 −1.218 −1.280 0.023 0.204 0.299 0.194 0.597 GPR56-Tango 0.377 −1.540 −2.197 −0.118 −0.020 −0.165 0.111 0.521 GPR6-Tango 0.145 −1.101 −1.019 0.023 0.076 0.234 0.312 0.315 GPR61-Tango −0.022 −1.750 −1.151 0.429 0.609 0.472 0.464 0.637 GPR62-Tango 0.324 −0.275 −0.402 −0.692 0.158 −0.245 −0.159 −0.091 GPR63-Tango 0.072 −0.693 −1.011 −0.328 −0.334 −0.216 0.042 −0.338 GPR64-Tango −0.072 −0.892 −0.839 −0.154 −0.447 −0.375 −0.030 −0.288 GPR65-Tango −0.121 −0.881 −0.488 0.026 0.029 −0.248 −0.222 −0.156 GPR68-Tango −0.509 −1.082 −0.891 −0.148 −0.115 −0.407 −0.656 −0.367 GPR75-Tango −0.342 −1.034 −0.347 0.140 0.231 −0.095 −0.225 −0.048 GPR77-Tango −0.946 −1.011 −1.106 −0.216 −0.372 0.047 −0.387 0.096 GPR78-Tango −0.124 −1.050 −0.575 0.395 0.590 0.204 0.028 0.103 GPR82-Tango −0.729 −2.014 −2.025 −0.024 −0.076 −0.061 −0.084 −0.015 GPR83-Tango −0.380 −0.930 −0.972 0.177 0.098 0.504 0.003 0.130 GPR84-Tango −0.245 −1.284 −1.562 −0.320 −0.221 0.123 −0.014 0.277 GPR85-Tango −0.115 −0.323 −0.700 −0.325 −0.445 0.167 0.193 −0.062 GPR87-Tango −0.030 −1.054 −1.050 −0.060 −0.208 −0.058 0.370 0.029 GPR88-Tango −0.063 −0.197 −0.298 0.176 −0.017 0.205 0.184 0.053 GPR97-Tango −0.479 −0.263 −0.553 −0.050 −0.156 0.041 −0.042 −0.002 GPRC5A-Tango −0.289 −1.452 −1.362 0.117 0.030 −0.121 −0.076 −0.065 GPRC5B-Tango −0.169 −1.354 −0.973 0.005 −0.069 0.195 −0.019 0.295 GPRC5C-Tango −0.180 −0.621 −0.376 0.162 0.132 0.343 −0.145 0.077 GPRC5D-Tango −0.363 −0.487 −1.119 −0.240 −0.105 −0.095 −0.123 0.038 GPRC6A-Tango −0.291 −0.627 −1.784 −0.409 −0.464 0.039 −0.074 0.050 GRM1-Tango −0.301 −0.662 −1.549 −0.590 −0.360 0.013 −0.439 0.073 GRM2-Tango −0.447 −0.339 −0.826 −0.081 −0.263 0.024 −0.400 0.064 GRM4-Tango −0.431 −0.955 −1.206 0.554 0.545 0.642 −0.024 0.609 GRM5-Tango −0.257 −0.908 −1.191 −0.075 0.008 −0.111 −0.455 0.019 GRM6-Tango −0.577 −0.895 −1.242 −0.481 −0.269 0.032 −0.216 0.000 GRM7-Tango −0.837 −1.306 −2.074 −1.269 −0.812 −0.374 −0.497 −0.446 GRM8-Tango −0.278 −0.877 −1.908 −0.171 −0.222 −0.146 −0.425 −0.017 GRPR-Tango −0.209 −0.829 −0.773 0.478 0.508 0.414 −0.313 0.016 HCA1-Tango 0.151 −1.055 −0.606 0.034 0.112 0.309 −0.171 0.160 HCA2-Tango 0.539 −1.132 −0.880 −0.172 −0.181 0.375 0.479 0.726 HCA3-Tango −0.069 −0.957 −0.564 −0.332 −0.277 0.584 0.108 0.344 HCTR1-Tango −0.033 −0.265 −0.874 0.434 0.000 −0.038 0.261 0.037 HCTR2-Tango 0.117 −0.585 −0.082 0.623 0.623 0.044 −0.376 0.170 HRH1-Tango 0.330 −1.768 −1.611 −0.073 −0.081 0.417 0.902 0.521 HRH2-Tango 0.469 0.123 −0.554 −0.068 −0.034 0.356 0.464 0.300 HRH3-Tango 0.505 −0.067 −0.798 −0.108 0.000 0.567 1.007 0.345 HRH4-Tango 1.046 −0.711 −0.883 −0.254 −0.231 0.127 1.023 0.052 HTR1A-Tango 0.323 −0.363 −0.976 0.138 0.172 0.106 0.393 0.262 HTR1B-Tango 0.117 −1.364 −1.453 −0.273 −0.217 0.112 0.504 0.330 HTR1D-Tango 0.038 −1.134 −1.039 −0.131 −0.251 0.205 0.259 0.124 HTR1E-Tango 0.177 −1.172 −1.706 0.131 0.113 0.368 0.549 0.547 HTR1F-Tango −0.369 −1.405 −1.024 0.408 0.081 −0.031 −0.423 −0.582 HTR2A-Tango −0.314 −0.949 −0.824 0.045 −0.012 0.127 −0.111 0.141 HTR2B-Tango −0.084 −2.261 −1.518 −0.133 −0.255 0.024 0.261 0.223 HTR2C-Tango −0.066 −0.911 −0.755 0.138 0.088 0.143 −0.094 0.165 HTR4-Tango −0.443 −1.132 −0.358 0.249 0.076 0.570 0.460 0.098 HTR5-Tango −0.150 −2.148 −1.510 0.394 0.216 0.339 0.141 0.391 HTR6-Tango 0.015 −0.545 −0.957 0.220 0.145 0.506 0.278 0.221 HTR7-Tango −0.019 −1.227 −1.084 0.592 0.572 0.007 −0.183 −0.372 KISSPEPTIN-R- 0.214 −0.347 −0.355 0.000 0.106 0.041 0.075 −0.310 Tango LHCGR-Tango −0.325 −1.178 −1.429 0.038 0.038 0.334 0.128 0.175 LPAR1-Tango 0.036 −1.827 −0.738 0.198 0.265 0.530 0.531 0.609 LPAR2-Tango −0.241 −0.892 −0.881 −0.289 −0.262 0.538 0.353 0.263 LPAR4-Tango −0.263 −1.788 −1.153 0.422 0.469 0.357 0.122 0.434 LPAR5-Tango −0.425 −0.862 −0.932 0.132 0.284 0.604 0.151 0.493 LPAR6-Tango 0.180 −2.171 −1.307 0.636 0.791 0.468 0.333 0.459 LTB4R-Tango −0.188 −0.526 −0.649 0.601 0.323 0.316 0.301 0.284 LTB4R2B-Tango 0.037 −1.157 −0.973 0.424 0.144 0.423 −0.079 0.126 MAS1-Tango 0.068 −1.003 −0.952 −0.062 −0.058 −0.066 0.255 0.084 MAS1L-Tango 0.040 −1.217 −1.356 0.064 0.201 0.006 0.001 −0.022 MC1R-Tango 0.175 −0.165 −0.270 −0.006 0.306 0.069 0.153 −0.039 MC2R-Tango −0.206 −0.283 0.255 0.514 0.316 0.339 0.052 0.130 MC3R-Tango −0.149 −1.105 −0.475 0.916 0.776 0.938 0.154 0.648 MC4R-Tango −0.429 −0.449 −0.642 −0.074 0.324 0.566 0.359 0.559 MC5R-Tango −0.079 −1.545 −1.523 −0.058 0.634 0.060 0.663 0.472 MCHR1-Tango −0.492 −1.100 −2.041 0.368 0.498 0.041 0.089 −0.053 MCHR2-Tango 0.000 −0.627 −0.807 0.000 0.391 0.093 −0.298 −0.150 MLNR-Tango 0.135 −0.011 −0.404 0.136 −0.206 −0.024 0.129 −0.048 MRGPRD-Tango −0.232 −1.672 −1.310 −0.037 −0.168 0.073 0.320 0.184 MRGPRE-Tango 0.139 −0.218 −0.494 −0.196 −0.189 0.169 0.349 0.112 MRGPRF-Tango −0.291 −1.021 −0.639 −0.152 −0.023 0.128 0.435 0.189 MRGPRG-Tango 0.025 −0.631 −1.155 −0.171 −0.066 0.071 0.216 −0.195 MRGPRX1-Tango −0.457 −1.484 −1.645 0.290 0.215 0.231 −0.026 0.193 MRGPRX2-Tango −0.319 −1.371 −1.219 0.127 0.206 0.127 0.194 −0.004 MRGPRX3-Tango −0.345 −0.457 −0.906 0.000 −0.019 0.423 −0.403 −0.296 MRGPRX4-Tango −1.261 −1.965 −2.479 −0.372 −0.483 −0.213 −0.717 0.003 MTNR1A-Tango −0.941 −1.577 −1.487 −0.380 −0.178 0.319 0.340 0.640 MTNR1B-Tango −0.895 −1.503 −1.391 −0.419 0.049 0.272 −0.017 0.351 NMBR-Tango −0.474 −0.903 0.000 0.077 0.284 0.603 0.115 0.202 NMUR1-Tango −0.475 −0.861 −1.667 −0.340 −0.082 0.202 −0.508 −0.341 NMUR2-Tango −1.129 −1.723 −1.867 −0.016 0.130 0.643 0.217 0.655 NPBW1-Tango 0.156 0.263 −0.241 0.310 0.260 0.064 −0.429 −0.573 NPBW2-Tango 0.027 −1.131 −1.162 −0.934 −0.891 −1.005 −0.683 −0.792 NPFF1-Tango 0.166 0.189 −0.447 0.235 −0.308 0.824 0.310 −0.142 NPFF2-Tango 0.118 −1.720 −0.839 0.016 0.189 0.656 −0.054 0.411 NPS-Tango −0.336 −1.130 −0.972 0.157 0.015 −0.035 −0.780 −0.086 NPY1R-Tango 0.174 −1.267 −0.680 0.120 0.196 0.496 −0.369 −0.045 NPY2R-Tango −0.005 −0.401 −1.169 0.124 0.200 0.271 −0.347 −0.167 NPY4R-Tango −0.085 −0.398 −0.823 −0.412 −0.051 −0.038 −0.191 −0.290 NPY5R-Tango −0.185 −0.350 −0.716 −0.415 −0.312 0.280 0.000 0.239 NTSR1-Tango −0.250 −1.746 −1.160 −0.866 −0.821 0.056 0.213 0.139 NTSR2-Tango −0.156 −1.088 −0.260 0.010 −0.450 0.057 −0.134 −0.311 OPN3-Tango −0.138 −0.197 0.000 0.110 0.229 0.602 0.472 −0.053 OPN5-Tango −0.897 −1.889 −1.016 −0.683 −0.506 0.167 0.281 0.806 OPRD1-Tango −0.835 −1.169 −0.916 −0.670 −0.584 0.094 −0.455 0.183 OPRK1-Tango 0.412 −1.115 −0.868 0.436 0.720 0.567 0.016 0.315 OPRL1-Tango 0.304 −0.634 −0.953 0.065 −0.164 0.046 0.080 −0.334 OPRM1-Tango −0.309 −0.394 −1.197 −0.198 −0.334 0.320 −0.275 −0.285 OXER1-Tango 0.287 −1.190 −0.747 −0.034 0.007 0.229 0.117 0.186 OXGR1-Tango −0.786 −1.726 −1.236 −0.259 −0.412 0.077 −0.299 0.130 OXTR-Tango −0.028 −0.171 0.059 −0.441 −0.094 0.436 0.225 −0.050 P2RY1-Tango 0.230 −1.894 −1.362 −0.680 −0.794 −0.078 0.162 0.232 P2RY10-Tango −0.282 −0.770 −0.687 −0.318 −0.467 0.481 0.050 0.160 P2RY11-Tango −0.327 −1.059 −0.556 −0.250 −0.232 0.574 0.010 −0.050 P2RY12-Tango −1.153 −1.061 −0.577 −0.008 −0.262 0.884 −0.013 1.407 P2RY13-Tango −0.164 −1.172 −1.387 −0.221 −0.320 0.187 0.273 0.385 P2RY14-Tango 0.059 −1.121 −1.345 −0.348 −0.380 0.102 0.270 0.235 P2RY2-Tango −0.420 −1.286 −1.087 −0.165 −0.421 0.251 0.182 0.353 P2RY4-Tango −0.366 −1.382 −1.663 −0.172 −0.161 0.396 0.154 0.484 P2RY6-Tango −0.189 −1.290 −1.133 −0.450 −0.496 0.269 0.151 0.362 P2RY8-Tango −0.157 −1.207 −1.483 −0.219 −0.073 0.205 −0.020 0.092 PK1-Tango 0.036 −0.487 −0.664 0.031 0.482 0.107 −0.155 −0.304 PK2-Tango −0.316 −0.354 −0.152 −0.317 0.044 0.050 −0.118 0.109 PRRP-Tango −0.085 −0.478 −0.195 −0.312 −0.205 0.109 0.058 0.359 PTAFR-Tango 0.030 −1.465 −0.937 −0.531 −0.672 0.092 0.380 0.447 PTGDR-Tango −0.433 −1.025 −0.607 −0.523 −0.466 0.295 0.047 0.113 PTGER1-Tango −0.053 −0.485 −0.267 0.482 0.261 0.408 −0.033 0.556 PTGER2-Tango −0.067 −0.531 −0.564 −0.202 0.202 0.721 0.661 0.251 PTGER3-Tango −0.263 −1.393 −0.819 0.087 0.316 0.284 0.340 0.463 PTGER4-Tango 0.354 −0.831 −0.987 0.927 0.886 0.557 0.573 0.577 PTGFR-Tango −0.489 −1.028 −0.985 0.345 0.740 0.770 0.423 0.668 PTGIR-Tango −0.225 −0.280 −1.163 0.048 −0.050 −0.272 −0.420 −0.722 PTH1R-Tango −0.226 −0.436 −1.228 0.029 0.097 0.499 0.435 0.201 PTH2R-Tango −0.500 −0.892 −1.586 −0.403 −0.231 0.387 −0.009 0.327 QRFP-Tango −0.418 −0.408 −0.868 −0.398 −0.322 0.187 −0.174 0.187 RXFP1-Tango −0.231 −1.416 −1.188 −0.042 −0.763 0.148 −0.147 −0.129 RXFP2-Tango −0.468 −1.601 −1.842 −0.338 −0.245 0.193 −0.063 0.404 RXFP3-Tango −0.784 −0.544 −1.527 −0.212 −0.372 −0.085 −0.264 −0.270 RXFP4-Tango −0.535 −0.555 −1.076 −0.772 0.000 −0.085 −0.085 −0.063 S1PR1-Tango −0.619 −0.758 0.955 −0.352 −0.492 0.903 0.850 0.798 S1PR2-Tango −0.322 −1.517 −0.255 0.173 −0.032 0.481 0.388 0.423 S1PR3-Tango −0.510 −1.042 −0.245 0.242 0.080 0.754 0.123 0.485 S1PR4-Tango −1.062 −1.944 −0.368 −0.797 −0.813 −0.125 0.029 0.267 S1PR5-Tango −0.232 −0.074 −0.812 −0.189 −0.778 0.258 −0.315 −0.289 SCTR-Tango −0.310 −1.000 −0.243 −0.255 0.016 −0.023 0.159 0.054 SSTR1-Tango −0.422 −1.107 −1.507 0.503 0.395 0.720 0.160 0.698 SSTR2-Tango −0.374 −0.986 −0.247 0.447 0.318 0.422 −0.474 0.000 SSTR3-Tango 0.216 −0.553 −0.213 0.118 0.468 0.435 0.200 −0.180 SSTR4-Tango −0.193 −1.165 −1.603 0.405 0.405 0.165 −0.140 0.369 SSTR5-Tango 1.335 −0.100 −0.428 1.085 1.229 0.258 0.504 −0.029 SUCNR1-Tango 3.978 1.204 0.480 0.614 0.449 −0.119 3.054 −0.547 TA1-Tango −0.122 −0.993 −0.495 0.441 0.223 0.273 −0.140 0.247 TAAR2-Tango −0.239 −0.922 −0.584 0.101 0.101 0.150 0.071 0.205 TAAR5-Tango −0.081 −0.328 −1.137 −0.429 −0.297 −0.462 −0.242 −0.473 TAAR6-Tango −0.380 −0.864 −1.487 −0.189 −0.220 −0.489 −0.559 −0.354 TAAR8-Tango −0.293 −0.912 −1.473 −0.671 −0.411 0.100 −0.131 −0.197 TAAR9-Tango −0.167 −0.880 −1.384 −0.311 −0.381 0.149 −0.024 0.169 TACR1-Tango −0.211 −0.839 −1.018 −0.190 −0.157 0.229 0.570 0.133 TACR2-Tango −0.046 −0.745 −0.209 0.017 −0.262 0.298 0.356 0.397 TACR3-Tango −0.449 −0.909 −0.766 0.067 0.000 0.259 −0.066 0.194 TBXA2R-Tango −0.225 −0.413 −0.340 −0.064 0.099 0.317 0.045 −0.191 TSHR-Tango −0.578 −1.170 −1.804 −0.065 0.187 −0.112 −0.825 −0.178 UTS2R-Tango −0.269 −0.310 −0.836 −0.020 0.184 −0.133 −0.309 −0.647 VIPR1-Tango −0.505 −1.528 −1.306 −0.104 0.489 0.177 0.403 0.758 VIPR2-Tango 1.353 −0.050 0.467 0.298 0.649 0.531 1.343 1.464 Bifidobac- Bifidobac- Bifidobac- Bifidobac- Bifidobac- Bifidobac- Bifidobac- PRESTO-Tango Eggerthela terium terium terium terium terium terium terium with GMM lenta spp UC-1# UC-2# UC-3# UC-4# UC-5# UC-6# ADCYAP1R1- 0.114 0.510 0.141 −0.456 0.082 0.438 −0.302 0.213 Tango ADORA1-Tango −0.333 0.423 −0.340 −0.863 −0.291 0.356 0.160 −0.091 ADORA2A-Tango −0.663 0.694 −0.589 −0.223 0.076 0.309 −0.343 −0.256 ADORA2B-Tango −0.239 0.093 −0.057 −0.258 −0.268 −0.079 −0.418 0.024 ADORA3-Tango −0.077 0.905 0.518 −0.248 0.383 0.508 0.380 0.387 ADRA1A-Tango −0.211 0.554 0.029 −0.188 −0.005 0.214 −0.205 −0.120 ADRA1B-Tango −0.371 0.651 −0.009 −0.620 −0.676 0.263 −0.232 −0.853 ADRA1D-Tango −0.376 0.423 −0.309 −0.329 −0.691 0.335 −0.443 −0.834 ADRA2A-Tango 0.033 0.182 0.964 0.427 0.302 0.000 −0.533 0.745 ADRA2B-Tango 0.000 −0.306 −0.144 −0.178 0.000 −0.160 −1.178 −0.515 ADRA2C-Tango −0.291 0.733 0.505 −0.115 0.335 0.000 −1.133 −0.258 ADRB1-Tango 0.200 1.124 0.465 0.082 −0.292 −0.122 −0.577 0.551 ADRB2-Tango −0.234 0.555 −0.195 −0.228 0.065 −0.030 −0.360 −0.234 ADRB3-Tango 0.023 0.684 0.057 0.146 −0.411 −0.111 −0.136 0.225 AGTR1-Tango −0.091 0.256 −0.179 −0.306 −0.627 0.349 −0.331 −0.131 AGTR2-Tango 0.238 0.570 −0.148 −0.095 −0.133 0.030 −0.494 −0.065 APJ-Tango −0.168 0.486 −0.413 −0.010 0.131 −0.154 −0.073 0.164 AVPR1A-Tango −0.499 0.682 0.262 0.092 0.323 0.267 −0.124 0.428 AVPR1B-Tango −0.055 0.318 −0.339 −0.255 −0.207 0.166 −0.180 −0.062 AVPR2-Tango 0.228 0.466 0.109 −0.111 −0.331 0.297 −0.230 0.496 BB3-Tango 0.287 0.371 −0.815 −0.656 −0.340 0.348 −0.159 −0.539 BDKBR1-Tango 0.037 0.444 −0.428 −0.256 −0.524 0.321 −0.387 0.115 BDKBR2-Tango 0.430 0.974 −0.259 −0.217 −0.442 0.261 0.016 0.882 C3AR1-Tango 0.438 0.280 −0.710 −0.813 −0.533 0.280 −0.265 0.239 C5A-Tango −0.339 0.723 −0.023 0.230 0.313 0.463 −0.357 0.286 CALCRb-Tango −0.348 0.413 0.090 −0.037 −0.129 0.152 −0.628 0.177 CALCRL-Tango −0.250 0.246 0.009 −0.006 0.045 0.128 −0.341 −0.362 CASR-Tango −0.569 0.850 −0.054 −0.338 0.110 0.482 0.133 −0.662 CCKAR-Tango 0.239 0.856 −0.203 −0.166 0.134 0.264 0.110 0.145 CCKBR-Tango −0.031 0.621 −0.466 −0.091 −0.303 0.052 −0.334 −0.006 CCR10-Tango −0.162 −0.084 −0.269 0.025 −0.374 −0.208 −0.459 0.152 CCR2-Tango −0.206 0.456 −0.228 −0.346 −0.231 0.200 −0.248 0.189 CCR3-Tango −0.188 0.660 0.063 −0.596 −0.311 0.561 0.161 0.238 CCR4-Tango −0.379 0.820 −0.420 −0.242 −0.346 0.474 0.000 −0.417 CCR5-Tango −0.339 0.832 −0.461 −0.062 0.210 0.298 −0.443 −0.100 CCR6-Tango −0.270 0.706 −0.330 −0.470 −0.420 0.030 −0.315 0.107 CCR7-Tango −0.125 0.452 −0.727 −0.115 −0.608 0.137 −0.022 −0.619 CCR8-Tango −0.767 0.044 −0.395 −0.103 −0.263 −0.528 −1.062 −0.360 CCRL2-Tango −0.631 1.163 −0.341 −0.213 −0.263 0.193 −0.155 −0.077 CD97-Tango −0.426 0.368 −0.680 −0.459 −0.404 0.280 −0.284 −0.968 CHRM1-Tango −0.604 0.291 −0.176 0.155 0.234 0.210 −0.814 −0.284 CHRM2-Tango 0.164 0.612 −0.485 −0.126 0.568 0.090 −0.120 −0.195 CHRM3-Tango −0.153 0.501 −0.164 0.052 0.173 0.232 −0.277 −0.493 CHRM4-Tango −0.129 0.432 −0.115 −0.076 −0.086 0.175 −0.391 −0.142 CHRM5-Tango 0.070 0.725 −0.334 0.003 0.297 0.260 −0.163 0.025 CMKLR1-Tango −0.190 0.619 −0.141 −0.033 −0.071 0.232 −0.174 0.479 CNR1-Tango −0.048 0.657 −0.369 −0.324 0.105 0.097 −0.311 −0.213 CNR2-Tango −0.201 0.536 −0.496 −0.412 −0.334 0.058 −0.369 0.132 CRHR1-Tango −0.040 0.270 0.057 −0.722 −0.044 0.170 −0.373 −0.040 CRHR2-Tango −0.334 0.557 −0.178 −0.252 0.318 −0.232 −0.579 0.230 CX3C1-Tango 0.447 0.537 −0.985 −0.255 −0.520 0.026 −0.072 0.318 CXCR1-Tango −0.875 0.475 0.038 0.354 0.354 0.361 −0.370 −0.064 CXCR2-Tango −0.152 0.560 0.090 −0.273 0.074 0.248 −0.177 0.244 CXCR3-Tango 0.127 0.486 −0.138 0.357 0.130 0.040 −0.453 0.127 CXCR4-Tango 0.424 0.995 −0.098 0.124 0.076 0.627 0.033 0.221 CXCR5-Tango 0.223 0.703 0.184 −0.263 −0.129 0.303 −0.072 0.284 CXCR6-Tango 0.500 −0.209 −0.260 −0.079 −0.263 −0.093 −0.335 0.455 CXCR7-Tango 0.035 −0.155 −0.642 −0.635 −0.334 0.193 −0.372 −0.480 CYSLTR1-Tango −0.078 0.167 −0.414 −0.051 0.281 0.297 −0.169 −0.232 CYSLTR2-Tango −0.620 0.496 0.265 0.463 0.071 0.451 −1.212 −0.101 DRD1-Tango −0.342 0.743 −0.315 0.240 0.155 0.875 −0.605 0.362 DRD2-Tango −0.913 0.952 0.170 1.420 −0.293 1.091 0.049 −0.016 DRD3-Tango −1.303 0.733 −0.503 0.876 0.098 1.628 −0.360 0.302 DRD4-Tango −0.526 1.186 −0.369 0.521 0.305 1.328 0.594 −0.328 DRD5-Tango −0.234 −0.293 −0.687 −0.203 −0.378 −0.420 −0.984 0.154 EDNRA-Tango 0.037 0.696 −0.390 −0.448 0.032 0.294 −0.055 0.242 EDNRB-Tango −0.306 0.349 −0.571 −0.114 −0.160 0.157 −0.078 −0.012 ELTD1-Tango −0.438 0.254 −0.978 −0.665 −0.028 0.326 0.076 −0.608 F2R-Tango −0.192 0.497 −0.594 −0.501 −0.089 0.064 −0.343 0.392 F2RL1-Tango −0.165 0.445 −0.553 −0.371 −0.039 0.153 −0.316 0.018 F2RL2-Tango −0.118 0.854 −0.052 −0.537 −0.089 0.474 0.101 −0.114 F2RL3-Tango −0.202 0.644 −0.484 −0.740 −0.242 0.318 −0.201 −0.194 FFA1-Tango 0.118 0.671 −0.256 −0.101 0.172 0.502 0.720 0.219 FFA2-Tango −0.067 0.641 −0.025 0.095 0.316 0.450 0.251 0.164 FFA3-Tango 0.210 0.817 −0.022 −0.244 0.034 0.286 0.167 0.256 FPR1-Tango 0.203 −0.127 −0.609 −0.113 0.197 0.988 −0.283 −0.587 FPR2-Tango 0.106 0.571 −0.200 0.095 0.278 0.369 −0.072 0.153 FPR3-Tango 0.056 0.478 −0.280 0.018 0.142 0.324 0.043 −0.093 FSHR-Tango 0.324 0.569 −0.420 −0.081 0.396 0.556 0.126 −0.020 GABBR1-Tango −0.313 0.320 −0.103 −0.033 −0.109 0.244 −0.596 0.008 GAL1-Tango 0.073 −0.044 −0.790 0.267 −0.670 −0.328 −0.184 −0.399 GAL2-Tango −0.071 0.721 −0.234 0.265 0.428 0.721 −0.420 0.089 GAL3-Tango −0.354 −0.057 −0.303 −0.870 0.194 −0.136 −0.355 0.219 GCGR-Tango −0.050 0.431 −0.240 −0.043 0.339 0.377 −0.040 0.347 GHRHR-Tango 0.257 0.214 −0.422 −0.155 0.084 −0.063 −0.751 0.738 GHSR-Tango 0.159 0.488 −0.636 −0.667 −0.435 0.343 −0.110 0.533 GIPR-Tango −0.072 −0.090 −0.724 −0.567 −0.244 −0.141 −0.861 0.519 GLP1R-Tango −0.063 0.508 −0.451 −0.651 0.035 0.218 0.390 0.110 GLP2R-Tango −0.247 0.336 −0.158 −0.009 0.107 0.125 −0.139 0.324 GNRHR-Tango −0.106 0.170 0.086 −0.111 0.000 0.347 −0.229 −0.266 GPBA-Tango 0.206 0.165 −0.384 −0.094 0.108 0.000 −0.339 −0.304 GPER-Tango −0.178 0.131 −0.113 −0.225 −0.225 0.476 −0.461 0.128 GPR1-Tango 0.068 0.382 −0.392 −0.075 −0.071 0.386 −0.317 −0.280 GPR101-Tango −0.008 0.181 −0.506 0.157 0.131 0.275 −0.175 0.221 GPR110-Tango 0.019 0.365 −0.312 −0.723 −0.206 0.251 −0.332 −0.358 GPR111-Tango −0.191 0.193 −0.194 −0.643 −0.586 0.093 −0.636 −0.163 GPR113-Tango −0.262 0.141 −0.348 −0.306 −0.057 0.107 −0.180 −0.164 GPR114-Tango 0.018 −0.024 −0.015 0.141 0.230 −0.234 −0.491 −0.087 GPR115-Tango −0.104 0.352 −0.030 −0.547 −0.133 0.263 0.009 −0.331 GPR116-Tango −0.293 0.263 0.009 −0.531 −0.419 0.288 −0.089 −0.366 GPR119-Tango −0.225 0.280 0.066 0.028 −0.119 0.139 −0.473 −0.091 GPR12-Tango 0.134 0.566 −0.253 0.163 −0.299 0.446 −0.347 0.342 GPR120-Tango −0.144 0.260 0.057 −0.397 −0.256 0.349 −0.503 −0.293 GPR123-Tango 0.005 0.604 0.238 −0.394 0.432 0.354 −0.185 0.296 GPR124-Tango −0.368 0.577 0.172 −0.824 −1.003 0.442 0.117 −0.778 GPR125-Tango −0.442 0.060 0.017 −0.875 −0.919 −0.017 −0.310 −0.519 GPR126-Tango −0.210 0.275 −0.199 −0.678 −0.598 −0.005 −0.439 0.192 GPR132-Tango −0.402 0.821 0.094 −0.906 −0.169 0.525 −0.015 −0.204 GPR133-Tango −0.019 0.669 −0.089 −0.499 −0.157 0.541 −0.078 −0.149 GPR135-Tango −0.252 0.078 −0.163 −0.458 −0.363 −0.097 −0.902 0.583 GPR141-Tango −0.522 0.533 −0.009 −0.925 −0.806 0.381 −0.138 0.205 GPR142-Tango −0.573 0.771 −0.382 −0.940 −0.549 0.278 −0.115 −0.696 GPR143-Tango −0.528 0.861 0.044 −0.467 0.364 0.577 0.280 −0.670 GPR144-Tango −0.569 0.465 −0.158 −0.477 −0.381 0.485 −0.141 −0.786 GPR146-Tango −0.745 0.531 0.410 −1.082 −0.420 0.812 0.531 0.133 GPR148-Tango −0.477 0.093 −0.333 −0.591 −0.951 0.658 0.341 −0.828 GPR149-Tango −0.070 0.790 0.017 0.371 0.425 0.693 0.515 −0.902 GPR15-Tango −0.152 0.726 −0.186 0.501 0.471 0.692 0.033 −0.444 GPR150-Tango −0.778 0.181 −0.405 −0.220 −0.420 0.599 −0.881 0.170 GPR151-Tango −0.478 0.784 −0.087 0.167 0.361 0.784 0.260 −0.343 GPR152-Tango −0.060 0.148 0.369 −0.258 0.003 0.098 −0.130 −0.244 GPR153-Tango 0.241 0.149 0.367 −0.191 0.102 0.239 −0.061 0.000 GPR156-Tango 0.052 −0.287 0.464 −0.286 −0.296 −0.149 −0.244 0.059 GPR157-Tango −0.066 0.209 0.173 −0.295 −0.338 −0.087 −0.407 −0.407 GPR158-Tango −0.147 0.265 0.271 −0.126 0.062 0.244 −0.677 −0.126 GPR160-Tango 0.041 0.686 0.143 −0.137 0.276 0.377 −0.117 −0.266 GPR161-Tango −0.047 0.422 0.132 −0.268 −0.191 0.317 0.035 −0.484 GPR162-Tango −0.197 −0.105 −0.239 −0.449 −0.330 −0.134 −0.471 −0.257 GPR17-Tango −0.074 0.445 0.370 0.380 0.408 0.315 0.438 −0.778 GPR171-Tango −0.201 0.706 0.731 −0.081 0.131 0.474 0.058 −0.420 GPR173-Tango −0.047 −0.047 −0.055 −0.186 −0.157 0.201 −0.675 0.070 GPR174-Tango −0.254 1.075 −0.027 −0.225 −0.425 0.550 0.152 −0.254 GPR18-Tango −0.175 0.448 −0.370 −0.182 −0.531 0.256 −0.368 −0.125 GPR182-Tango 0.042 0.770 0.011 0.074 0.089 0.531 −0.055 −0.506 GPR183-Tango 0.102 0.425 −0.224 −0.184 0.096 0.353 0.064 0.040 GPR19-Tango −0.032 0.935 0.139 −0.483 0.138 0.473 0.219 −0.573 GPR20-Tango 0.030 0.497 0.261 −0.361 −0.191 0.404 0.234 0.200 GPR21-Tango −0.039 0.714 0.215 0.361 0.124 0.087 −0.142 0.104 GPR22-Tango 0.376 0.371 0.025 −0.318 0.123 0.398 0.362 −0.335 GPR25-Tango −0.179 0.184 −0.099 0.156 −0.373 0.275 −0.082 −0.078 GPR26-Tango 0.348 0.399 −0.408 −0.649 −0.578 0.197 −0.070 0.090 GPR27-Tango 0.014 0.484 0.547 0.225 −0.343 0.214 −0.142 0.125 GPR3-Tango −0.080 0.565 −0.501 0.105 −0.214 0.665 0.433 0.174 GPR31-Tango −0.098 0.807 −0.467 −0.185 −0.467 0.907 0.205 0.149 GPR32-Tango 0.263 0.203 −0.232 0.055 0.028 −0.064 −0.292 0.097 GPR34-Tango −0.182 0.500 −0.301 −0.447 −0.045 0.404 −0.197 0.009 GPR35-Tango −0.221 0.632 0.193 −0.189 −0.085 0.214 0.043 0.254 GPR37-Tango −0.179 0.702 0.068 −0.220 −0.291 0.436 −0.021 −0.037 GPR37L1-Tango 0.106 0.500 0.067 −0.592 −0.353 0.320 −0.155 −0.059 GPR39-Tango −0.177 0.158 −0.186 −0.452 −0.436 0.039 −0.496 −0.170 GPR4-Tango −0.078 0.940 0.083 −0.399 −0.455 0.607 0.084 0.013 GPR44-Tango 0.090 0.310 −0.233 −0.404 −0.200 0.222 −0.603 0.279 GPR45-Tango −0.224 0.292 −0.503 −0.315 0.222 0.427 −0.350 0.595 GPR50-Tango −0.216 1.007 0.592 −0.392 0.109 0.468 0.345 −0.143 GPR52-Tango −0.084 0.314 0.098 0.580 −0.599 −0.045 −0.449 0.259 GPR55-Tango 0.086 0.812 0.425 0.274 0.341 0.396 0.116 0.125 GPR56-Tango −0.090 0.619 0.229 −0.859 −0.528 0.884 0.468 −0.020 GPR6-Tango 0.044 1.132 0.375 0.136 0.058 0.767 0.233 0.020 GPR61-Tango −0.036 0.915 0.319 −0.163 −0.097 0.539 −0.423 0.038 GPR62-Tango 0.113 0.873 0.657 −0.455 −0.295 0.407 −0.405 0.340 GPR63-Tango −0.421 −0.255 0.320 0.037 0.209 0.003 −0.337 −0.490 GPR64-Tango −0.064 −0.180 0.166 −0.392 −0.020 −0.125 −0.346 −0.102 GPR65-Tango 0.091 0.365 0.125 0.023 0.255 0.136 −0.105 −0.064 GPR68-Tango 0.055 0.124 0.087 −0.571 −0.016 0.319 −0.165 −0.120 GPR75-Tango −0.176 0.565 0.138 0.213 0.238 0.236 −0.074 −0.135 GPR77-Tango −0.153 0.162 0.016 −0.227 −0.059 0.029 −0.745 −0.157 GPR78-Tango −0.197 0.856 0.212 0.080 −0.033 0.448 −0.077 −0.051 GPR82-Tango 0.048 0.500 −0.125 −0.551 −0.287 0.047 −0.222 0.084 GPR83-Tango 0.136 0.262 0.249 −0.581 0.126 0.149 0.101 −0.032 GPR84-Tango −0.033 0.483 0.020 −0.283 0.013 0.410 0.190 −0.552 GPR85-Tango −0.140 0.562 0.201 −0.218 −0.209 0.357 0.000 −0.154 GPR87-Tango −0.189 0.378 0.042 −0.029 −0.065 0.445 −0.153 0.051 GPR88-Tango 0.035 0.190 0.084 0.219 −0.057 0.106 −0.522 0.270 GPR97-Tango 0.144 0.535 0.209 −0.100 −0.190 0.215 −0.402 −0.510 GPRC5A-Tango −0.196 0.866 0.051 −0.482 0.042 0.679 0.350 −0.198 GPRC5B-Tango −0.004 0.888 0.008 −0.168 0.038 0.578 0.360 −0.539 GPRC5C-Tango −0.235 0.488 0.188 0.154 0.569 0.628 0.231 −0.335 GPRC5D-Tango −0.047 −0.042 0.230 −0.270 −0.229 0.056 −0.345 −0.208 GPRC6A-Tango 0.125 0.325 0.633 −0.471 −0.273 0.215 −0.284 −0.446 GRM1-Tango −0.006 0.288 −0.042 −0.556 −0.466 0.139 −0.360 −0.311 GRM2-Tango −0.306 0.211 0.175 −0.390 −0.269 −0.046 −0.457 −0.448 GRM4-Tango −0.119 0.879 0.426 −0.524 0.312 0.703 0.354 −0.356 GRM5-Tango −0.028 −0.118 0.159 −0.569 −0.261 −0.131 −0.726 −0.305 GRM6-Tango −0.427 −0.663 0.131 −0.332 −0.358 −0.478 −0.264 −0.876 GRM7-Tango −0.075 −0.468 0.084 −0.693 −0.682 −0.328 −0.752 −0.857 GRM8-Tango −0.140 −0.255 0.047 −0.534 −0.683 0.007 −0.363 −0.921 GRPR-Tango 0.348 0.406 0.066 −0.312 0.140 0.343 −0.060 0.200 HCA1-Tango −0.141 0.712 0.191 −0.297 0.198 0.381 −0.089 −0.421 HCA2-Tango −0.105 1.083 0.237 −0.017 0.241 0.892 0.387 −0.606 HCA3-Tango −0.280 1.059 0.000 −0.339 −0.095 0.842 0.253 −0.560 HCTR1-Tango 0.377 1.012 −0.387 −1.033 0.095 0.350 0.313 0.317 HCTR2-Tango −0.093 0.722 −0.234 0.078 0.705 0.377 −0.341 −0.497 HRH1-Tango 0.101 0.384 −0.347 −0.008 −0.038 0.370 0.061 −0.682 HRH2-Tango −0.080 0.277 0.193 0.404 −0.187 0.365 −0.086 −0.113 HRH3-Tango −0.374 −0.100 0.393 0.105 −0.327 −0.109 −0.874 −0.208 HRH4-Tango 0.492 0.393 −0.496 −0.345 −0.686 0.280 −0.327 0.221 HTR1A-Tango −0.162 0.974 0.300 0.051 −0.028 0.827 0.165 0.000 HTR1B-Tango 0.332 0.813 −0.236 −0.259 −0.178 0.598 0.239 −0.397 HTR1D-Tango −0.402 0.654 −0.381 −0.094 −0.139 0.480 −0.027 −0.221 HTR1E-Tango −0.266 0.742 −0.007 −0.206 −0.127 0.454 0.086 −0.264 HTR1F-Tango 0.105 0.619 −0.460 −0.168 0.226 0.435 0.066 −0.478 HTR2A-Tango −0.441 0.377 −0.353 −0.195 0.150 0.177 −0.359 −0.874 HTR2B-Tango −0.005 0.928 −0.141 −0.213 0.004 0.607 0.132 −0.766 HTR2C-Tango −0.290 0.318 0.174 −0.283 −0.287 0.073 −0.770 −0.703 HTR4-Tango 0.029 0.702 −0.415 0.099 0.032 0.242 −0.177 0.324 HTR5-Tango −0.195 0.701 0.000 0.052 0.295 0.550 0.070 0.110 HTR6-Tango −0.696 0.338 −0.280 −0.195 −0.499 0.350 −0.282 −1.044 HTR7-Tango −0.436 0.888 0.373 −0.430 −0.142 0.299 −0.004 −0.419 KISSPEPTIN-R- 0.000 −0.031 −0.397 0.030 −0.062 −0.094 −1.127 0.093 Tango LHCGR-Tango −0.543 1.003 −0.512 −0.669 −0.212 0.451 0.359 −0.634 LPAR1-Tango 0.123 0.997 −0.060 −0.244 0.392 0.735 0.280 −0.140 LPAR2-Tango −0.539 0.515 −0.042 −0.551 −0.165 0.218 −0.352 −0.153 LPAR4-Tango −0.178 0.886 0.057 −0.399 −0.010 0.277 −0.020 −0.290 LPAR5-Tango −0.622 0.634 0.122 −0.210 −0.082 0.380 −0.304 −0.733 LPAR6-Tango −0.059 0.799 0.165 −0.269 0.023 0.491 0.252 −0.817 LTB4R-Tango −0.140 0.259 0.267 −0.098 −0.435 0.135 −0.305 −0.520 LTB4R2B-Tango −0.152 0.816 −0.335 −0.144 0.210 0.552 0.384 −0.821 MAS1-Tango 0.351 0.387 −0.112 −0.293 −0.010 0.274 −0.373 −0.288 MAS1L-Tango −0.026 0.274 −0.302 −0.325 0.079 0.256 −0.051 −0.751 MC1R-Tango −0.262 0.131 −0.135 0.049 0.015 0.070 −0.475 −0.804 MC2R-Tango 0.041 0.432 −0.057 0.608 0.666 0.227 −0.537 −0.357 MC3R-Tango −0.292 0.884 −0.240 −0.053 0.160 0.213 −0.430 −0.371 MC4R-Tango −0.852 0.681 0.160 0.087 0.022 0.647 0.141 −0.325 MC5R-Tango 0.435 1.188 −0.606 −0.058 −0.189 0.415 −0.136 0.435 MCHR1-Tango 0.000 −0.808 0.150 −0.588 −0.413 −0.703 −1.192 −1.091 MCHR2-Tango −0.271 −0.413 0.115 −0.371 −0.511 −0.345 −0.602 −1.142 MLNR-Tango 0.085 −0.067 0.341 −0.357 −0.532 0.047 −0.272 −0.276 MRGPRD-Tango 0.043 0.019 −0.068 −0.307 −0.040 0.088 −0.142 −0.971 MRGPRE-Tango −0.144 0.056 0.327 −0.125 −0.009 −0.071 −0.345 −0.118 MRGPRF-Tango 0.052 0.581 −0.070 0.162 0.020 0.611 −0.307 −0.398 MRGPRG-Tango 0.050 −0.292 0.053 −0.239 −0.075 −0.485 −0.642 −0.310 MRGPRX1-Tango −0.071 0.064 −0.312 −0.801 −0.596 −0.417 −0.865 −0.720 MRGPRX2-Tango −0.653 0.043 0.065 0.174 0.352 0.121 0.052 −1.278 MRGPRX3-Tango −0.547 0.246 −0.275 −0.383 −0.095 0.034 −0.214 −0.426 MRGPRX4-Tango 0.182 0.062 0.012 −0.216 0.091 −0.494 −0.021 −1.103 MTNR1A-Tango 0.541 0.188 −0.250 −0.548 −0.259 0.219 −0.489 −0.365 MTNR1B-Tango −0.732 0.410 −0.146 −0.096 0.111 0.054 −0.643 −0.845 NMBR-Tango 0.131 0.262 −0.334 0.585 0.680 0.433 −0.523 −0.100 NMUR1-Tango 0.922 1.073 −0.539 −0.317 −0.374 0.883 −0.117 0.525 NMUR2-Tango −0.699 1.092 0.493 −0.415 −0.256 1.198 0.707 −0.195 NPBW1-Tango −0.115 0.758 −0.057 −0.241 0.077 0.501 0.625 0.187 NPBW2-Tango −0.046 0.426 0.093 −0.716 0.299 0.112 0.924 −0.708 NPFF1-Tango −0.095 −1.359 0.488 −0.261 −0.299 −0.966 −1.108 0.200 NPFF2-Tango −0.151 0.959 0.259 0.093 0.523 0.226 0.529 −0.066 NPS-Tango 0.123 0.076 −0.472 −0.504 −0.268 −1.212 −0.473 −0.506 NPY1R-Tango 0.094 0.539 −0.084 0.024 0.156 −0.902 −0.180 −0.073 NPY2R-Tango 0.067 0.895 0.454 −0.034 0.340 −1.628 0.507 −0.481 NPY4R-Tango 0.453 0.260 0.295 −0.297 −0.327 −0.098 −0.034 −0.073 NPY5R-Tango −0.058 0.357 0.262 0.098 0.636 0.236 0.295 −0.889 NTSR1-Tango 0.273 0.497 −0.323 0.819 0.974 0.216 0.078 −0.477 NTSR2-Tango −0.134 0.443 −0.322 0.531 0.452 0.234 −0.013 −0.462 OPN3-Tango −0.068 −0.207 0.280 0.465 0.146 −0.266 −0.266 −0.155 OPN5-Tango 0.198 0.540 0.026 0.199 0.918 0.231 0.347 −0.612 OPRD1-Tango −0.222 0.457 0.070 −0.192 −0.192 0.170 −0.673 −0.444 OPRK1-Tango −0.003 0.694 −0.109 −0.390 0.084 0.191 −0.319 −0.237 OPRL1-Tango −0.071 −0.194 −0.170 −0.588 −0.548 −0.064 −0.899 0.418 OPRM1-Tango −0.221 0.226 0.221 −0.303 0.348 0.398 −0.346 −0.082 OXER1-Tango 0.334 0.336 0.008 −0.107 0.547 0.163 0.122 −0.237 OXGR1-Tango −0.011 0.297 −0.018 −0.038 0.103 0.345 −0.069 −0.217 OXTR-Tango −0.060 0.457 0.120 0.459 0.027 0.384 −0.177 −0.210 P2RY1-Tango −0.038 0.592 −0.467 −0.433 −0.012 0.572 0.207 −0.811 P2RY10-Tango −0.063 0.284 −0.279 −0.357 −0.065 0.233 −0.308 −0.213 P2RY11-Tango −0.066 0.755 −0.446 −0.032 0.116 0.503 0.422 −0.262 P2RY12-Tango −0.239 1.486 0.357 0.218 0.578 0.564 0.381 0.501 P2RY13-Tango −0.098 0.232 0.219 −0.121 0.238 0.297 0.029 −0.252 P2RY14-Tango 0.063 −0.097 0.270 −0.165 0.105 −0.106 −0.134 −0.392 P2RY2-Tango −0.212 0.627 0.180 −0.403 −0.026 0.345 −0.003 −0.443 P2RY4-Tango 0.075 0.810 0.214 −0.739 −0.002 0.489 0.070 −0.338 P2RY6-Tango 0.134 0.186 −0.046 −0.261 −0.136 −0.122 −0.232 −0.473 P2RY8-Tango 0.333 0.160 −0.009 −0.734 −0.307 −0.037 −0.405 −0.494 PK1-Tango −0.081 0.017 −0.118 −0.541 −0.483 −0.049 −0.898 −0.907 PK2-Tango 0.000 0.430 0.021 0.025 0.114 0.515 0.074 0.000 PRRP-Tango −0.238 0.436 0.187 −0.044 0.487 0.365 0.118 −0.452 PTAFR-Tango 0.071 0.410 0.097 0.248 0.423 0.528 0.373 −0.914 PTGDR-Tango −0.346 0.646 −0.311 −0.196 −0.380 0.248 −0.305 −0.620 PTGER1-Tango 0.415 0.639 −0.122 −0.092 0.000 0.188 −0.216 −0.165 PTGER2-Tango 0.506 0.707 0.058 −0.071 −0.489 0.748 0.034 0.126 PTGER3-Tango −0.409 0.675 −0.211 0.016 0.380 0.675 0.385 0.118 PTGER4-Tango −0.422 0.902 0.005 0.326 −0.129 0.439 −0.027 −0.422 PTGFR-Tango −0.087 0.449 −0.716 −0.180 −0.386 0.819 0.220 −0.628 PTGIR-Tango −0.463 0.733 −0.076 −0.382 −0.640 0.913 0.699 −0.611 PTH1R-Tango 0.280 0.512 0.035 −0.363 −0.147 −0.166 −0.354 0.054 PTH2R-Tango −0.182 0.406 0.313 −0.316 −0.308 0.357 0.252 −0.852 QRFP-Tango −0.425 0.550 −0.072 −0.357 −0.347 0.474 −0.038 −0.425 RXFP1-Tango −0.400 0.049 −0.307 −0.266 −0.100 −0.264 −0.757 −0.689 RXFP2-Tango −0.243 0.489 0.067 −0.785 −0.159 0.210 0.296 −0.895 RXFP3-Tango −0.060 0.000 0.103 −0.595 −0.814 −0.012 −0.753 −0.572 RXFP4-Tango 0.399 0.148 −0.627 0.261 0.284 0.328 0.000 −0.627 S1PR1-Tango 0.067 0.608 0.111 0.888 1.598 0.447 0.242 −0.413 S1PR2-Tango −0.325 0.513 −0.254 0.734 0.726 0.263 0.047 −0.507 S1PR3-Tango 0.000 1.035 0.024 0.272 −0.075 0.208 0.238 −0.425 S1PR4-Tango 0.414 0.337 −0.101 0.260 0.124 0.048 −0.004 −0.813 S1PR5-Tango −0.073 0.681 −0.304 0.000 0.106 0.505 0.141 −0.376 SCTR-Tango −0.239 0.749 0.086 0.435 0.154 0.260 0.086 −0.358 SSTR1-Tango 0.485 0.592 −0.341 −0.482 −1.130 0.672 −0.541 0.127 SSTR2-Tango 0.062 0.239 0.050 0.445 0.333 0.405 0.221 −0.503 SSTR3-Tango 0.115 0.670 −0.080 0.000 0.556 0.564 0.564 0.047 SSTR4-Tango −0.092 0.322 0.428 −0.045 −0.100 −0.038 −0.399 −1.531 SSTR5-Tango 0.126 0.775 1.253 0.475 0.108 0.820 −0.287 0.541 SUCNR1-Tango −0.248 0.174 0.935 2.152 −0.505 0.776 −0.198 0.105 TA1-Tango −0.191 0.558 0.313 −0.386 0.110 0.513 −0.307 −0.460 TAAR2-Tango −0.064 0.340 −0.354 −0.090 −0.162 0.474 −0.802 −0.520 TAAR5-Tango −0.040 −0.896 −0.454 −0.443 −0.543 −0.731 −1.400 −0.491 TAAR6-Tango 0.066 −0.217 0.000 −0.629 −0.121 −0.112 −0.186 −0.703 TAAR8-Tango −0.216 −0.358 −0.144 −0.299 −0.132 −0.165 −0.291 −0.258 TAAR9-Tango 0.299 −0.140 −0.275 −0.477 −0.382 −0.246 −0.284 −0.452 TACR1-Tango 0.015 0.052 −0.030 −0.116 −0.021 0.041 −0.004 −0.601 TACR2-Tango −0.353 0.055 −0.082 −0.016 −0.045 0.084 −0.364 −0.366 TACR3-Tango −0.147 0.589 −0.034 0.028 0.257 0.388 0.258 −0.675 TBXA2R-Tango −0.149 −0.022 0.193 −0.095 −0.176 −0.125 −0.153 −0.080 TSHR-Tango −0.071 0.121 0.102 −0.849 −0.413 −0.257 0.061 −0.753 UTS2R-Tango −0.052 0.327 0.318 0.037 −0.266 −0.142 −0.160 −0.143 VIPR1-Tango −0.696 −0.786 0.042 −0.200 −0.200 −0.975 −1.097 −0.759 VIPR2-Tango −0.896 0.340 1.100 0.000 0.000 0.303 0.758 −0.615 PRESTO-Tango Bifidobacterium Bifidobacterium Bifidobacterium Morganella Morganella Morganella Morganella Morganella with GMM adolescentis-1# adolescentis-2# adolescentis-3# spp-1# spp-2# spp-3# spp-4# spp-5# ADCYAP1R1- −0.021 −0.014 −0.160 −0.862 −0.387 −0.531 0.025 −0.248 Tango ADORA1-Tango −0.262 −0.045 −0.393 −1.367 −0.177 −0.366 −0.237 −0.359 ADORA2A-Tango −0.178 −0.430 −0.270 −1.277 0.021 −0.017 0.209 −0.540 ADORA2B-Tango −0.146 −0.342 −0.152 −0.770 −0.376 −0.279 −0.089 −0.260 ADORA3-Tango 0.333 0.168 0.251 −0.347 −0.369 −0.549 −0.369 −0.416 ADRA1A-Tango −0.216 −0.203 −0.114 −0.623 −0.235 −0.285 0.012 0.098 ADRA1B-Tango −0.416 −0.327 0.294 −1.219 −0.538 −0.672 −0.891 −0.808 ADRA1D-Tango −0.748 −0.150 −0.138 −0.886 −0.229 −0.434 0.174 0.017 ADRA2A-Tango 0.073 0.136 −0.106 0.125 0.793 0.816 1.246 1.216 ADRA2B-Tango 0.078 −0.055 −0.515 −1.660 0.252 0.662 0.258 0.679 ADRA2C-Tango 0.065 −0.531 −0.176 −1.431 0.000 1.482 1.186 1.042 ADRB1-Tango 0.028 −0.077 0.138 −0.266 0.553 0.669 0.230 0.429 ADRB2-Tango −0.057 −0.308 −0.564 −2.203 −0.799 −0.285 0.133 −0.528 ADRB3-Tango −0.235 −0.411 −0.292 −1.644 −0.595 0.125 −0.471 0.106 AGTR1-Tango −0.164 −0.259 −0.147 −1.490 −0.262 0.015 0.382 0.593 AGTR2-Tango −0.104 −0.091 0.288 −0.508 0.246 −0.242 0.442 0.442 APJ-Tango 0.312 0.010 0.096 −1.393 −0.255 −0.442 −0.318 0.208 AVPR1A-Tango 0.547 0.296 0.280 −2.619 −0.642 −0.514 −0.520 −0.595 AVPR1B-Tango −0.160 −0.150 −0.098 −1.362 −0.328 −0.365 −0.188 −0.427 AVPR2-Tango −0.227 −0.222 −0.247 −1.982 −0.232 −0.221 −0.347 −0.270 BB3-Tango −0.302 −0.231 −0.276 −0.806 −0.469 0.071 −0.520 −0.476 BDKBR1-Tango −0.409 −0.198 −0.116 −1.122 0.176 −0.018 −0.341 −0.026 BDKBR2-Tango −0.230 −0.267 0.247 −1.050 −0.061 −0.197 0.021 0.191 C3AR1-Tango −0.416 −0.451 −0.067 −0.723 −0.314 0.083 0.219 −0.054 C5A-Tango 0.106 0.024 0.175 −1.125 −0.273 −0.262 −0.591 −0.647 CALCRb-Tango −0.073 −0.139 −0.043 −1.348 −0.191 −0.023 −0.698 −0.183 CALCRL-Tango −0.015 −0.135 −0.117 −1.096 −0.143 −0.012 −0.242 −0.224 CASR-Tango 0.221 0.112 −0.379 −1.494 −0.905 −0.503 −1.313 −1.405 CCKAR-Tango 0.037 −0.030 0.294 −1.243 −0.401 −0.449 −0.503 −0.534 CCKBR-Tango −0.089 −0.179 0.003 −1.218 −0.009 −0.106 −0.559 −0.650 CCR10-Tango −0.264 −0.578 −0.402 −1.876 −0.394 −0.580 −0.549 −0.564 CCR2-Tango −0.038 −0.038 0.106 −1.648 −0.373 0.147 −0.853 −0.817 CCR3-Tango −0.155 0.100 0.225 −2.027 0.139 −0.065 0.213 0.445 CCR4-Tango 0.022 −0.008 0.090 −1.684 −1.247 −0.153 −0.815 −0.318 CCR5-Tango 0.149 −0.372 −0.036 −1.419 0.107 −0.566 0.308 −0.454 CCR6-Tango −1.048 −0.567 0.345 −1.169 0.097 0.064 0.540 0.666 CCR7-Tango −0.288 −0.020 −0.681 −1.422 −0.532 −0.471 0.878 −0.565 CCR8-Tango 0.258 −0.289 −0.487 −2.045 −0.412 −0.435 0.080 0.466 CCRL2-Tango −0.289 −0.021 −0.297 −1.122 −0.339 −0.045 −0.077 0.415 CD97-Tango −0.705 −0.705 −0.302 −0.177 0.262 0.327 −0.621 −0.451 CHRM1-Tango 0.000 −0.102 0.266 −0.384 −0.069 −0.081 0.463 0.355 CHRM2-Tango 0.117 0.022 −0.492 −0.717 −0.495 −0.147 −0.101 −0.207 CHRM3-Tango 0.019 −0.148 −0.130 −0.372 −0.012 0.058 0.628 0.521 CHRM4-Tango 0.078 −0.055 0.135 −1.099 0.059 −0.310 0.229 0.265 CHRM5-Tango −0.014 −0.091 −0.298 −1.106 −0.389 −0.162 −0.024 −0.312 CMKLR1-Tango −0.046 −0.330 −0.032 −1.368 −0.432 −0.386 −0.170 −0.342 CNR1-Tango −0.021 −0.030 −0.172 −1.180 −0.589 −0.478 −0.011 −0.241 CNR2-Tango −0.537 −0.580 −0.046 −1.461 −0.272 −0.381 −0.077 −0.058 CRHR1-Tango −0.170 0.000 0.261 −1.396 −0.399 −0.730 0.796 −0.341 CRHR2-Tango 0.433 0.239 0.417 −1.546 −0.298 −0.919 −0.095 0.533 CX3C1-Tango 0.050 −0.477 −0.069 −1.420 0.358 −0.643 −0.034 0.180 CXCR1-Tango 0.302 −0.033 0.149 −1.902 0.069 0.023 0.942 0.336 CXCR2-Tango −0.336 −0.129 −0.212 −0.503 0.530 0.828 1.120 1.024 CXCR3-Tango 0.010 0.165 −0.330 −1.438 −0.139 −0.152 0.326 0.252 CXCR4-Tango 0.036 −0.203 0.031 −1.075 −0.141 −0.117 0.371 0.357 CXCR5-Tango −0.263 −0.511 0.037 −0.475 −0.297 0.294 0.365 0.123 CXCR6-Tango 0.155 −0.229 −0.147 −1.505 0.087 −0.133 1.000 1.129 CXCR7-Tango −0.154 0.130 −0.428 −1.316 −0.271 −0.076 0.609 0.456 CYSLTR1-Tango 0.321 0.068 0.180 −1.455 0.209 0.475 0.646 0.599 CYSLTR2-Tango 0.224 0.540 −0.058 −0.852 0.062 0.510 0.615 0.500 DRD1-Tango 0.143 −0.050 −0.442 −2.319 −0.680 0.875 1.191 1.025 DRD2-Tango 0.025 −0.259 −0.659 2.541 3.090 3.791 4.037 4.196 DRD3-Tango −0.655 0.098 −1.091 1.890 1.730 3.375 3.895 3.843 DRD4-Tango −1.087 −0.052 −1.000 2.421 1.149 2.979 2.966 3.231 DRD5-Tango −0.015 0.015 −0.367 −1.975 −0.386 −0.556 −0.029 0.000 EDNRA-Tango 0.292 0.220 0.182 −2.107 −0.583 −0.548 −0.176 0.081 EDNRB-Tango 0.069 −0.012 −0.316 −1.840 −0.342 −0.064 −0.326 −0.353 ELTD1-Tango −0.022 −0.013 −0.271 −2.942 −0.422 0.041 −0.481 −0.587 F2R-Tango −0.038 −0.046 0.294 −3.510 −0.442 −0.339 −0.356 −0.165 F2RL1-Tango 0.042 −0.020 0.098 −3.035 −0.586 −0.493 −0.227 −0.073 F2RL2-Tango −0.059 −0.030 −0.067 −3.104 −0.864 −0.402 −0.426 −0.547 F2RL3-Tango 0.038 −0.044 0.064 −2.733 −0.556 −0.603 −0.114 −0.090 FFA1-Tango 0.343 0.318 −0.224 −1.180 −0.173 −0.094 −0.223 −0.158 FFA2-Tango 0.549 0.566 −0.271 −1.681 −0.498 0.121 −0.079 −0.065 FFA3-Tango 0.239 0.322 −0.056 −1.266 −0.581 −0.050 0.256 0.245 FPR1-Tango 0.543 0.420 −0.033 −0.471 0.495 0.841 1.034 1.068 FPR2-Tango 0.131 0.106 −0.239 −1.179 −0.212 0.202 0.458 0.386 FPR3-Tango 0.386 0.357 −0.121 −1.524 −0.265 −0.388 0.006 0.136 FSHR-Tango 0.268 0.370 −0.132 −1.948 −0.858 −0.435 −0.495 −0.437 GABBR1-Tango 0.098 0.154 −0.111 −0.149 0.581 0.516 0.589 0.576 GAL1-Tango −0.411 −0.553 −0.395 −2.499 −0.219 −0.799 −0.501 −0.383 GAL2-Tango 0.166 0.496 0.362 −2.824 −0.671 −1.016 0.885 0.885 GAL3-Tango −0.300 −0.300 0.322 −1.617 −0.650 −1.198 −1.040 −0.772 GCGR-Tango 0.372 0.154 −0.140 −1.878 −0.777 −0.534 −0.258 −0.301 GHRHR-Tango −0.042 −0.244 0.228 −1.560 −0.195 −0.635 −0.606 −0.575 GHSR-Tango 0.043 −0.144 −0.127 −2.110 −0.842 −0.614 −0.086 0.270 GIPR-Tango −0.132 −0.341 0.043 −2.087 −0.476 −0.893 −0.335 −0.040 GLP1R-Tango 0.039 −0.086 0.369 −2.126 −0.519 −0.960 −0.743 −0.330 GLP2R-Tango 0.407 0.091 −0.279 −1.840 −0.059 −0.647 −0.075 0.414 GNRHR-Tango −0.054 −0.667 −0.307 −1.217 −0.627 −0.333 0.200 0.250 GPBA-Tango −0.395 −0.330 0.140 −0.924 0.260 −0.391 0.645 1.075 GPER-Tango −0.267 −0.246 0.246 −1.463 −0.339 0.040 0.000 0.330 GPR1-Tango 0.256 0.033 −0.317 −1.195 −0.459 −0.579 −0.341 −0.311 GPR101-Tango −0.188 0.074 −0.117 −0.749 −0.077 −0.494 0.069 −0.072 GPR110-Tango −0.410 −0.473 0.019 −1.462 −0.566 −0.488 −0.480 −0.480 GPR111-Tango −0.643 −0.511 −0.432 −1.176 −0.493 −0.126 −0.225 0.258 GPR113-Tango −0.255 0.219 −0.510 −1.285 −0.820 −0.609 −0.441 −0.185 GPR114-Tango 0.239 0.261 −0.172 −0.852 −0.135 −0.443 −0.368 −0.053 GPR115-Tango 0.095 0.142 −0.398 −1.433 −0.603 −0.532 −0.598 −0.553 GPR116-Tango −0.232 −0.255 −0.326 −1.131 −0.315 −0.256 −0.509 −0.358 GPR119-Tango −0.082 −0.035 −0.230 −1.510 −0.268 −0.126 −0.018 0.141 GPR12-Tango −0.537 −0.363 −0.132 −1.745 0.266 0.015 0.194 0.236 GPR120-Tango −0.347 −0.490 −0.190 −0.754 0.126 −0.164 −0.198 0.054 GPR123-Tango 0.339 0.345 0.151 −1.147 −0.494 −0.600 0.131 0.188 GPR124-Tango −0.503 −0.612 −0.748 −1.302 −0.748 −0.259 −0.640 −0.578 GPR125-Tango −0.334 −0.383 −0.980 −1.027 −0.687 −0.711 −0.140 −0.289 GPR126-Tango −0.323 −0.366 −0.857 −1.516 −0.145 −0.359 −0.307 −0.437 GPR132-Tango −0.066 −0.011 −0.643 −1.841 −0.985 −0.844 −0.736 −0.762 GPR133-Tango 0.004 0.031 −0.826 −1.622 −0.947 −0.546 −0.864 −0.683 GPR135-Tango −0.041 −0.297 −0.316 −1.647 −0.350 −0.440 −0.267 −0.267 GPR141-Tango −0.925 −0.937 −0.598 −0.738 0.023 0.525 0.411 0.203 GPR142-Tango −0.678 −0.696 −0.722 −1.612 −0.602 −0.828 −0.133 0.017 GPR143-Tango 0.396 0.141 0.074 −2.535 −0.769 −0.704 −0.368 −0.461 GPR144-Tango 0.024 −0.242 −0.449 −2.271 −0.803 −0.174 −0.523 −0.840 GPR146-Tango −0.241 −0.485 0.117 −1.780 0.358 0.812 −0.217 0.481 GPR148-Tango −0.649 −0.904 0.341 −1.387 −0.404 −0.170 0.147 1.496 GPR149-Tango 0.454 0.106 −1.300 −2.534 −0.522 −0.160 0.523 0.208 GPR15-Tango 0.040 0.435 −0.305 −2.273 −0.326 0.177 0.133 0.403 GPR150-Tango −0.542 −0.420 −0.054 −1.063 0.052 0.144 0.282 1.200 GPR151-Tango 0.388 0.117 −0.663 −1.873 −0.198 −0.471 −0.302 −0.559 GPR152-Tango 0.060 0.034 0.010 −0.983 −0.480 −0.664 −0.591 −0.644 GPR153-Tango 0.173 0.233 0.244 −1.114 −0.601 −0.404 −0.173 −0.051 GPR156-Tango −0.082 −0.027 −0.189 −0.180 0.026 −0.706 0.082 0.250 GPR157-Tango −0.295 −0.231 −0.148 −0.916 −0.387 −0.143 −0.478 0.028 GPR158-Tango −0.050 −0.068 0.058 −1.272 −0.100 −0.394 −0.570 −0.440 GPR160-Tango 0.238 0.327 −0.362 −0.936 −0.683 −0.194 0.000 0.160 GPR161-Tango −0.106 −0.123 −0.518 −1.177 −0.195 −0.030 0.246 0.550 GPR162-Tango −0.400 −0.230 −0.701 −1.383 −0.608 −0.299 −0.287 −0.424 GPR17-Tango 0.684 0.693 −0.658 −0.358 −0.288 0.048 0.544 0.415 GPR171-Tango 0.355 0.212 −0.475 −0.263 −0.378 0.265 0.013 0.159 GPR173-Tango −0.420 −0.157 −0.441 −1.135 −0.298 −0.047 0.000 0.076 GPR174-Tango −0.120 −0.497 −0.187 −1.503 −0.088 0.164 0.365 0.239 GPR18-Tango −0.192 −0.477 −0.581 −1.757 −0.508 −0.344 −0.112 0.320 GPR182-Tango 0.179 0.123 −0.542 −1.088 −0.652 −0.080 −0.126 −0.005 GPR183-Tango −0.027 −0.180 −0.541 −1.381 −0.336 −0.205 −0.051 −0.295 GPR19-Tango 0.170 0.121 −0.518 −0.851 −0.847 −0.371 −0.580 −0.480 GPR20-Tango −0.361 −0.312 −0.753 −1.303 0.047 0.000 0.511 −0.621 GPR21-Tango −0.110 0.124 −0.146 −1.314 0.064 0.150 0.075 0.340 GPR22-Tango −0.118 −0.489 −0.234 −1.339 −0.038 −0.270 0.405 0.547 GPR25-Tango −0.415 −0.016 −0.131 −1.540 0.031 −0.190 −0.220 0.371 GPR26-Tango −0.241 −0.696 −0.402 −1.851 −0.054 −0.288 0.093 −0.374 GPR27-Tango 0.166 −0.118 0.221 −0.857 0.330 0.310 0.880 0.829 GPR3-Tango 0.223 0.156 −0.247 −1.470 −0.498 0.079 0.478 0.423 GPR31-Tango −0.040 −0.090 −0.419 −1.670 −0.465 0.192 0.339 0.154 GPR32-Tango 0.266 0.290 −0.353 −1.689 0.000 −0.710 0.064 0.100 GPR34-Tango 0.239 −0.143 −0.993 −1.366 −0.385 −0.145 0.125 0.300 GPR35-Tango 0.047 0.127 −0.298 −2.127 −0.502 −0.614 −0.152 −0.085 GPR37-Tango −0.108 −0.026 −0.155 −1.302 −0.147 −0.401 0.143 0.242 GPR37L1-Tango −0.372 −0.379 −0.330 −1.620 −0.188 −0.631 −0.367 −0.374 GPR39-Tango −0.399 −0.309 −0.259 −1.698 −0.212 −0.546 −0.636 −0.263 GPR4-Tango −0.136 −0.222 −0.144 −1.464 −0.061 −0.349 0.123 0.236 GPR44-Tango −0.410 −0.283 −0.534 −0.997 −0.202 −0.298 0.194 −0.141 GPR45-Tango 0.293 −0.087 −0.515 −2.048 −0.551 −0.144 −0.455 −0.737 GPR50-Tango 0.252 0.214 0.477 −1.364 −0.264 0.061 0.720 0.593 GPR52-Tango −0.401 −0.222 −0.215 −0.695 0.812 −0.102 0.805 0.565 GPR55-Tango 0.084 0.337 0.012 −1.227 −0.359 −0.215 0.551 0.372 GPR56-Tango −0.271 −0.387 −0.016 −1.318 −0.379 0.114 0.173 0.217 GPR6-Tango 0.063 0.221 −0.286 −1.572 0.284 0.242 0.236 0.010 GPR61-Tango 0.373 0.334 −0.038 −1.380 −0.146 −0.598 0.020 −0.018 GPR62-Tango 0.096 0.119 −0.264 −0.615 −0.248 0.245 −0.269 −0.790 GPR63-Tango 0.010 −0.225 −0.166 −0.862 −0.554 −0.769 −0.437 −0.351 GPR64-Tango −0.022 −0.187 −0.338 −1.198 −0.384 −0.939 −0.009 −0.349 GPR65-Tango 0.414 0.453 0.032 −1.372 0.015 −0.473 −0.129 −0.332 GPR68-Tango 0.211 −0.111 −0.217 −1.639 −0.442 −0.760 −0.163 −0.649 GPR75-Tango 0.137 0.258 0.139 −1.441 0.115 −0.436 0.229 0.000 GPR77-Tango 0.080 0.035 −0.184 −1.661 −0.318 −0.529 −0.525 −0.468 GPR78-Tango 0.272 0.077 −0.159 −1.695 0.053 0.043 −0.096 −0.571 GPR82-Tango −0.048 −0.164 −0.362 −2.910 −0.478 −0.410 −0.082 −0.311 GPR83-Tango 0.148 −0.056 0.130 −1.472 −0.044 −0.680 −0.176 −0.234 GPR84-Tango 0.056 0.051 0.079 −1.156 −0.066 −0.351 −0.220 −0.340 GPR85-Tango −0.121 −0.130 0.023 −0.929 0.133 0.059 0.023 0.051 GPR87-Tango −0.139 0.036 0.304 −0.620 0.129 −0.034 0.246 0.588 GPR88-Tango 0.030 −0.168 −0.165 −0.804 0.277 −0.297 0.121 0.346 GPR97-Tango 0.182 0.228 −0.032 −1.045 −0.018 −0.203 −0.030 0.036 GPRC5A-Tango 0.069 0.114 0.142 −1.396 0.041 −0.337 −0.391 −0.144 GPRC5B-Tango 0.190 −0.010 0.002 −1.344 −0.235 −0.109 −0.150 −0.487 GPRC5C-Tango 0.508 0.258 0.693 −0.732 0.497 −0.343 −0.002 0.098 GPRC5D-Tango −0.515 −0.448 0.320 −1.124 −0.122 −0.946 −0.230 −0.230 GPRC6A-Tango −0.431 −0.557 0.469 −1.150 −0.016 −0.827 −0.347 −0.481 GRM1-Tango −0.559 −0.628 0.049 −1.376 −0.162 −0.512 −0.327 −0.052 GRM2-Tango −0.541 −0.449 0.062 −1.132 0.050 −0.282 −0.073 −0.236 GRM4-Tango 0.293 0.267 0.579 −1.356 −0.108 −0.163 −0.319 −0.314 GRM5-Tango −0.325 −0.354 0.229 −1.146 −0.362 −0.923 −0.347 −0.309 GRM6-Tango −0.597 −0.607 −0.514 −0.957 −0.250 −0.717 0.089 0.039 GRM7-Tango −0.804 −0.749 0.316 −1.681 −0.067 −0.980 −0.434 −0.198 GRM8-Tango −0.534 −0.913 0.042 −0.956 −0.261 −0.434 0.158 0.267 GRPR-Tango −0.023 −0.531 0.485 −1.659 −0.097 −0.446 0.420 −0.219 HCA1-Tango 0.028 −0.399 −0.149 −1.726 −0.023 −0.019 −0.138 −0.108 HCA2-Tango 0.366 0.190 0.806 −1.168 0.495 −0.332 0.574 0.930 HCA3-Tango −0.056 −0.212 0.000 −1.831 −0.440 0.037 0.097 0.158 HCTR1-Tango −0.156 −0.310 −0.376 −0.745 −0.036 0.760 0.248 0.682 HCTR2-Tango 0.267 0.152 −0.094 −0.953 −0.471 −0.078 0.122 0.234 HRH1-Tango −0.033 0.277 0.092 −1.485 0.311 −0.449 0.770 0.552 HRH2-Tango 0.026 0.197 0.274 −0.316 0.045 0.230 0.669 0.607 HRH3-Tango −0.381 −0.213 0.050 1.247 1.510 0.805 2.290 2.201 HRH4-Tango −0.670 −0.582 −0.656 0.447 1.187 1.163 1.909 2.005 HTR1A-Tango 0.298 −0.042 0.096 −1.166 0.581 0.797 0.906 0.711 HTR1B-Tango −0.011 0.029 0.255 −1.618 0.120 −0.129 0.767 0.762 HTR1D-Tango −0.206 −0.041 −0.164 −1.400 0.061 0.110 0.705 0.472 HTR1E-Tango −0.022 −0.003 −0.176 −1.088 −0.132 −0.385 0.342 0.278 HTR1F-Tango 0.357 0.203 0.536 −1.610 −0.482 −0.345 0.685 0.465 HTR2A-Tango 0.138 0.100 0.037 −1.489 −0.316 −0.169 0.063 0.188 HTR2B-Tango 0.085 0.105 −1.322 −1.148 −0.148 −0.234 0.263 0.287 HTR2C-Tango 0.140 −0.072 −1.476 −1.607 −0.042 −0.430 −0.240 −0.176 HTR4-Tango 0.125 0.089 −1.597 −1.676 −0.156 0.195 0.181 0.648 HTR5-Tango 0.318 0.157 −1.249 −1.972 −0.053 −0.021 0.670 0.386 HTR6-Tango −0.380 −0.195 −1.114 −1.252 0.264 0.508 −0.047 −0.047 HTR7-Tango 0.093 −0.078 −2.023 −0.548 0.060 −0.098 0.268 0.424 KISSPEPTIN-R- −0.031 −0.330 −0.092 −1.962 −0.308 −0.127 −1.000 −0.333 Tango LHCGR-Tango −0.541 −0.410 0.562 −2.806 −0.426 −0.472 −0.486 −0.540 LPAR1-Tango 0.191 0.256 0.591 −3.406 −0.037 0.046 0.165 0.036 LPAR2-Tango 0.006 −0.141 0.246 −2.775 −0.434 −0.459 −0.503 −0.481 LPAR4-Tango 0.064 0.047 0.344 −2.953 −0.022 −0.280 −0.334 −0.521 LPAR5-Tango −0.046 −0.039 −0.078 −2.420 0.007 0.065 −0.142 −0.012 LPAR6-Tango 0.106 0.057 0.165 −1.407 −0.166 −0.164 0.500 0.553 LTB4R-Tango −0.022 −0.194 −0.468 −1.629 0.000 −0.212 0.338 0.455 LTB4R2B-Tango 0.118 0.316 0.486 −1.530 0.022 −0.457 0.086 −0.153 MAS1-Tango −0.025 0.198 0.331 −1.505 0.017 0.189 −0.051 0.000 MAS1L-Tango 0.069 0.059 0.432 −1.646 −0.224 −0.806 −0.588 −0.461 MC1R-Tango 0.082 0.173 0.160 −1.223 0.047 −0.323 −0.198 −0.384 MC2R-Tango 0.160 0.435 0.374 −1.605 −0.214 −0.089 0.894 0.458 MC3R-Tango −0.115 0.160 0.391 −1.439 −0.117 −0.284 −0.122 0.112 MC4R-Tango 0.229 −0.069 0.110 −0.840 0.532 0.225 0.503 0.503 MC5R-Tango −0.333 0.135 0.229 −2.578 0.754 −0.260 0.768 0.514 MCHR1-Tango −0.372 −0.672 −0.850 −2.015 −1.113 −1.061 −0.298 −0.269 MCHR2-Tango −0.037 −0.395 −0.568 −0.913 −0.568 −0.780 −0.071 −0.098 MLNR-Tango 0.012 −0.170 −0.126 −1.172 0.030 −0.364 0.177 0.117 MRGPRD-Tango 0.088 0.056 −0.171 −1.924 −0.517 −0.402 0.100 0.229 MRGPRE-Tango 0.034 0.110 −0.442 −1.053 −0.024 −0.429 0.216 0.090 MRGPRF-Tango 0.148 0.003 0.711 −0.687 0.423 −0.258 0.323 0.433 MRGPRG-Tango −0.340 −0.358 0.018 −0.678 −0.552 −0.667 −0.177 0.050 MRGPRX1-Tango −0.922 −0.703 −0.739 −1.386 −0.879 −0.693 0.454 0.261 MRGPRX2-Tango 0.126 0.083 −0.357 −0.935 −0.189 −0.588 0.371 0.450 MRGPRX3-Tango −0.463 −0.672 −0.157 −1.316 −0.204 −0.457 0.155 −0.029 MRGPRX4-Tango −0.062 −0.079 −0.231 −2.759 −0.569 −1.255 −0.289 −0.474 MTNR1A-Tango −0.227 0.084 0.389 −2.351 0.415 −0.547 0.255 −0.771 MTNR1B-Tango 0.218 0.376 0.216 −1.344 0.530 −0.330 0.875 0.643 NMBR-Tango 0.831 0.742 0.695 −1.362 −0.123 −0.876 0.105 0.022 NMUR1-Tango −0.626 −0.140 −0.071 −1.591 −0.361 −0.179 0.042 −0.766 NMUR2-Tango 0.329 0.236 0.357 −2.693 −0.313 −0.596 −0.272 −0.480 NPBW1-Tango −0.485 −0.298 −0.798 −0.613 −0.077 0.340 −0.047 0.350 NPBW2-Tango 0.148 0.201 −0.880 −0.932 −0.230 0.579 0.413 0.196 NPFF1-Tango 0.221 0.265 −0.124 −0.850 0.138 −1.408 0.214 −0.411 NPFF2-Tango 0.543 0.407 0.337 −2.348 −0.125 −0.755 −0.392 −0.183 NPS-Tango −0.239 −0.361 −0.865 −1.779 −0.478 −1.363 −0.407 −1.153 NPY1R-Tango 0.285 0.389 −0.528 −2.304 −0.077 −1.066 −0.499 −0.642 NPY2R-Tango −0.782 0.571 −0.358 −0.913 −0.183 −0.754 −0.365 −0.628 NPY4R-Tango −0.710 −0.523 −0.589 −1.260 −0.260 −0.671 −0.065 0.000 NPY5R-Tango 0.636 0.591 −0.117 −0.789 0.132 −0.123 0.096 −0.035 NTSR1-Tango 1.125 0.938 −0.011 −2.297 −0.111 −0.250 −0.029 0.019 NTSR2-Tango 0.572 0.849 0.354 −2.188 −0.111 0.112 −0.565 −0.635 OPN3-Tango 0.772 0.873 0.132 −1.160 0.031 −0.474 −0.382 −0.347 OPN5-Tango 1.172 1.249 0.015 −2.310 −0.286 −1.200 −0.107 −0.113 OPRD1-Tango −0.020 −0.058 −0.228 −1.962 −0.308 −1.218 0.098 −0.125 OPRK1-Tango 0.020 −0.074 0.538 −1.777 0.006 −0.630 0.000 −0.152 OPRL1-Tango 0.032 −0.213 −0.261 −1.152 −0.405 −0.575 0.505 0.420 OPRM1-Tango 0.333 0.031 0.886 −1.267 0.577 −0.617 −0.291 0.000 OXER1-Tango 0.489 0.614 0.644 −1.392 −0.260 −1.331 −0.861 −0.348 OXGR1-Tango 0.335 0.203 0.094 −2.241 −0.174 −1.158 −0.488 −0.280 OXTR-Tango 0.145 0.213 0.000 −0.850 0.180 −0.149 0.034 0.000 P2RY1-Tango 0.200 0.233 0.315 −2.084 −0.206 −0.173 −0.054 −0.107 P2RY10-Tango −0.041 −0.145 0.121 −1.635 −0.132 −0.616 −0.015 −0.392 P2RY11-Tango 0.331 −0.075 0.140 −2.242 −0.261 −0.033 −0.237 −0.627 P2RY12-Tango 0.684 0.678 0.630 −1.816 −0.060 −1.005 0.535 0.768 P2RY13-Tango 0.293 0.200 −0.110 −0.915 −0.392 −0.400 0.167 0.275 P2RY14-Tango 0.382 0.204 −0.340 −0.999 −0.710 −0.609 0.041 0.338 P2RY2-Tango 0.218 0.059 −0.261 −1.691 −0.483 −0.334 −0.145 −0.150 P2RY4-Tango −0.021 −0.073 −0.148 −2.040 −0.886 −0.761 −0.027 0.007 P2RY6-Tango 0.080 0.144 −0.362 −1.877 −0.196 −0.600 0.240 0.165 P2RY8-Tango −0.149 −0.125 −0.234 −1.669 −0.827 −0.917 0.007 0.074 PK1-Tango −0.312 −0.459 −0.491 −1.302 −0.267 −0.811 −0.089 −0.162 PK2-Tango 0.339 0.220 −0.247 −0.799 −0.262 −0.255 −0.573 −0.376 PRRP-Tango 0.304 0.088 −0.707 −1.325 −0.274 −0.038 0.436 0.242 PTAFR-Tango 0.385 0.383 0.096 −1.303 −0.307 −0.114 0.449 0.646 PTGDR-Tango −0.183 −0.446 −0.291 −1.682 −0.072 0.076 0.017 −0.017 PTGER1-Tango −0.022 −0.515 −0.087 −1.410 0.167 0.092 −0.170 −0.122 PTGER2-Tango −0.155 −0.026 −0.391 −1.328 −0.055 −0.336 0.698 0.397 PTGER3-Tango 0.146 0.348 0.023 −1.478 −0.212 −0.126 0.598 0.654 PTGER4-Tango 0.008 0.092 0.197 −1.536 0.317 0.048 0.296 0.204 PTGFR-Tango −0.155 −0.045 −0.338 −2.019 −0.085 −0.106 0.405 −0.127 PTGIR-Tango −0.113 −0.291 −0.348 −1.193 −0.450 −0.057 0.545 0.697 PTH1R-Tango −0.125 −0.376 −0.329 −1.147 −0.122 −0.461 0.650 0.671 PTH2R-Tango −0.072 −0.563 −0.746 −1.199 −0.832 −0.573 0.379 0.308 QRFP-Tango −0.227 −0.546 −0.140 −1.669 −0.140 0.000 0.353 0.092 RXFP1-Tango −0.151 −0.405 −0.410 −2.068 −0.495 −0.017 −0.419 −0.543 RXFP2-Tango −0.125 −0.351 −0.750 −1.538 −1.033 −0.804 −0.183 −0.208 RXFP3-Tango −0.460 −0.773 −0.312 −1.206 −0.084 −0.555 −0.198 −0.428 RXFP4-Tango 0.284 0.027 −0.533 −1.018 −0.560 0.000 0.117 0.060 S1PR1-Tango 1.595 1.306 −0.251 −0.704 −0.500 0.030 0.308 0.064 S1PR2-Tango 1.018 0.991 −0.245 −2.812 −0.643 0.017 0.101 −0.619 S1PR3-Tango 0.548 0.508 −0.157 −1.835 −0.119 0.154 0.591 −0.285 S1PR4-Tango 0.406 0.729 −0.601 −2.428 −0.231 −0.699 0.089 −0.035 S1PR5-Tango −0.094 0.491 −0.240 −0.993 −0.219 −0.087 0.000 0.148 SCTR-Tango 0.435 0.000 −0.081 −1.490 −0.136 −0.305 0.807 0.951 SSTR1-Tango −0.710 −0.252 −0.766 −1.541 −0.241 −0.286 0.462 0.263 SSTR2-Tango 0.939 1.072 −0.754 −1.368 0.026 −0.660 −1.000 −0.333 SSTR3-Tango 0.258 0.827 −0.129 −1.406 0.052 −0.243 0.745 0.623 SSTR4-Tango −0.537 −0.208 −0.939 −0.097 −0.042 −0.554 0.601 0.722 SSTR5-Tango −0.359 −0.033 −0.657 0.730 0.614 0.665 0.639 −0.111 SUCNR1-Tango −0.455 −0.483 −0.529 3.142 2.409 2.875 2.663 2.606 TA1-Tango 0.283 0.248 −0.300 −1.276 −0.188 −0.133 0.538 0.461 TAAR2-Tango 0.177 0.063 −0.250 −1.073 −0.076 −0.256 0.256 −0.020 TAAR5-Tango −0.457 −0.642 −0.512 −1.404 −0.189 −1.031 0.011 −0.513 TAAR6-Tango −0.136 −0.290 −0.059 −0.611 −0.881 −0.810 −0.447 −0.514 TAAR8-Tango −0.677 −0.385 −0.143 −1.075 −0.508 −0.952 −0.488 −0.488 TAAR9-Tango −0.481 −0.473 0.080 −1.166 −0.641 −0.826 −0.455 −0.373 TACR1-Tango −0.012 0.144 −0.092 −0.559 −0.255 −0.344 0.191 0.300 TACR2-Tango 0.067 −0.107 −0.558 −0.842 −0.448 −0.319 −0.036 −0.302 TACR3-Tango 0.331 0.194 0.074 −0.842 −0.104 −0.467 −0.165 −0.206 TBXA2R-Tango −0.064 −0.164 −0.446 −0.993 −0.364 −0.502 −0.427 −0.045 TSHR-Tango −0.427 −0.184 0.073 −1.295 −0.660 −1.062 −0.933 −0.619 UTS2R-Tango −0.243 0.136 −0.262 −1.064 −0.125 −0.445 −0.909 −1.364 VIPR1-Tango −0.031 −0.236 −0.299 −1.059 −0.453 −1.604 −1.074 −0.448 VIPR2-Tango 1.700 2.072 0.556 0.778 −0.112 0.731 1.840 2.165 Morganella Morganella morganii- morganii- (Enterobac- (Enterobac- (Enterobac- (Enterobac- (Enterobac- PRESTO-Tango Morganella ATCC ATCC teria)UC/ teria)UC/ teria)UC/ teria)UC/ teria)UC/ with GMM spp-6# 25830 49948 UC-1# UC-2# UC-3# UC-4# UC-5# ADCYAP1R1- −0.371 0.026 0.093 −0.937 −1.444 0.108 −0.892 −0.549 Tango ADORA1-Tango −0.116 0.085 −0.037 −1.873 −2.027 −0.896 −1.359 −1.677 ADORA2A-Tango 0.094 −0.146 −0.071 −1.246 −1.627 −0.942 −0.488 −0.602 ADORA2B-Tango −0.364 −0.155 −0.342 −0.982 −1.525 −0.295 −0.820 −0.858 ADORA3-Tango −0.376 −0.125 −0.265 −0.837 −1.150 0.560 −0.670 −0.431 ADRA1A-Tango −0.252 −0.247 −0.226 −1.041 −1.386 −0.136 −0.684 −0.681 ADRA1B-Tango −0.555 −0.545 −1.010 −1.143 −1.643 −0.648 −0.775 −0.942 ADRA1D-Tango −0.374 −0.540 −0.647 −1.021 −1.455 −1.019 −0.570 −0.306 ADRA2A-Tango 1.760 1.651 1.806 −3.386 −3.281 0.249 −1.144 −0.892 ADRA2B-Tango 0.349 −0.037 0.488 −3.332 −2.654 −1.514 −1.194 −1.163 ADRA2C-Tango 1.361 0.618 1.167 −1.405 −1.515 −0.121 −0.267 −0.531 ADRB1-Tango 0.461 1.040 1.302 −1.875 −1.497 0.379 −0.954 −0.585 ADRB2-Tango −0.239 −0.103 −0.425 −1.712 −2.332 −0.304 −0.563 −1.813 ADRB3-Tango 0.423 0.353 0.334 −1.153 −1.203 0.129 −0.987 −0.670 AGTR1-Tango 0.429 −0.012 −0.027 −1.375 −1.826 0.230 −1.164 −0.990 AGTR2-Tango 0.045 −0.143 −0.001 −1.233 −1.298 0.012 −1.506 −0.770 APJ-Tango −0.222 0.009 0.294 −1.961 −1.843 −0.931 −1.364 −1.237 AVPR1A-Tango −0.390 0.342 0.173 −1.845 −2.306 −0.451 −1.227 −0.972 AVPR1B-Tango −0.005 −0.255 −0.358 −1.485 −1.780 −0.192 −1.298 −0.670 AVPR2-Tango 0.031 0.021 0.059 −2.189 −2.077 −0.189 −1.769 −1.197 BB3-Tango 0.037 −0.077 −0.083 −1.286 −1.429 −0.549 −1.336 −0.473 BDKBR1-Tango 0.240 −0.020 0.220 −0.608 −1.269 −0.109 −1.345 −0.524 BDKBR2-Tango −0.013 0.784 0.467 −0.567 −0.897 0.081 −1.048 −0.257 C3AR1-Tango −0.296 0.214 0.360 −0.940 −1.174 −0.327 −1.472 −1.216 C5A-Tango −0.185 −0.309 −0.318 −0.924 −1.600 0.079 −1.026 −0.571 CALCRb-Tango −0.634 −0.087 −0.222 −0.894 −1.368 −0.359 −0.448 −0.736 CALCRL-Tango −0.148 0.202 −0.294 −0.655 −1.012 −0.166 −0.288 −0.397 CASR-Tango −0.456 −0.785 −1.061 −0.886 −1.158 −0.367 −0.805 −0.648 CCKAR-Tango −0.296 −0.118 −0.261 −0.563 −0.735 −0.138 −0.578 −0.405 CCKBR-Tango 0.226 −0.100 0.318 −1.024 −1.291 −0.499 −0.725 −0.847 CCR10-Tango −0.206 0.166 0.126 −1.314 −1.350 −1.067 −0.502 −0.922 CCR2-Tango −0.805 0.056 0.000 −1.344 −1.268 −0.803 −0.362 −0.588 CCR3-Tango −0.455 −0.379 −0.334 −2.076 −2.362 −0.930 −1.973 −1.273 CCR4-Tango −0.744 −0.666 −0.831 −1.440 −1.845 −0.076 −0.947 −0.666 CCR5-Tango −0.384 −0.068 −0.533 −1.350 −1.459 0.153 −0.844 −0.163 CCR6-Tango 0.756 0.141 0.260 −0.729 −1.535 0.248 −1.282 −0.896 CCR7-Tango −0.549 −0.031 −0.206 −1.399 −1.903 −0.098 −1.203 −0.328 CCR8-Tango −0.502 −0.767 −0.375 −1.906 −2.741 −0.076 −0.958 −0.360 CCRL2-Tango 1.096 0.000 0.728 −1.382 −1.774 0.536 −0.493 −0.707 CD97-Tango 1.045 −0.261 0.094 −1.455 −1.673 −0.255 −1.000 −0.639 CHRM1-Tango 0.496 0.060 0.198 −1.106 −1.232 −0.096 −0.372 −0.310 CHRM2-Tango 0.532 0.168 0.354 −1.170 −1.041 0.007 −1.012 −0.254 CHRM3-Tango 0.497 −0.071 0.382 −1.370 −1.776 0.173 −1.064 −0.609 CHRM4-Tango 0.120 −0.069 −0.048 −0.708 −1.373 −0.012 −0.642 −0.773 CHRM5-Tango −0.110 0.007 0.000 −0.938 −1.106 0.048 −0.574 −0.234 CMKLR1-Tango −0.058 0.022 0.055 −1.417 −1.641 0.259 −0.674 −0.773 CNR1-Tango −0.207 −0.455 −0.618 −1.413 −1.543 −0.233 −1.105 −0.454 CNR2-Tango −0.155 0.326 −0.064 −1.327 −1.882 −0.020 −1.084 −0.720 CRHR1-Tango −0.292 0.100 0.100 −1.801 −2.005 −0.229 −0.480 −0.480 CRHR2-Tango −0.028 −0.461 −0.304 −0.989 −1.211 0.228 −0.889 −0.659 CX3C1-Tango −0.728 0.664 0.528 −1.160 −1.482 −0.750 −0.792 −1.196 CXCR1-Tango 0.902 0.125 0.305 −1.451 −1.729 −0.515 −0.160 −0.033 CXCR2-Tango 1.691 1.101 1.282 −0.699 −1.775 −0.087 −0.278 −0.048 CXCR3-Tango 0.457 0.043 0.169 −1.621 −2.372 −0.339 −0.786 −0.990 CXCR4-Tango 0.121 0.189 0.204 −0.924 −1.309 −0.083 −0.405 −0.913 CXCR5-Tango 0.357 0.172 0.447 −0.930 −1.580 0.276 −0.714 −1.065 CXCR6-Tango 0.244 0.500 1.019 −0.555 −1.519 −0.231 −0.819 −0.434 CXCR7-Tango 0.449 0.418 0.616 −1.593 −1.282 −0.737 −1.744 −0.566 CYSLTR1-Tango 1.076 0.403 0.505 −1.551 −1.996 −0.750 −0.478 0.460 CYSLTR2-Tango 0.655 −0.017 0.142 −1.155 −1.722 −0.325 −0.037 0.193 DRD1-Tango 0.564 1.913 1.759 −2.292 −3.201 −0.956 −1.349 −0.676 DRD2-Tango 3.630 4.089 4.073 −2.278 −2.826 −0.045 −1.922 −0.869 DRD3-Tango 3.863 2.887 4.096 −1.863 −2.326 0.385 −0.387 −0.983 DRD4-Tango 2.678 2.627 3.559 −2.415 −2.065 0.455 −1.200 −1.087 DRD5-Tango −0.560 0.007 0.007 −1.411 −1.748 −1.367 −0.672 −0.624 EDNRA-Tango −0.443 −0.129 −0.349 −0.990 −1.298 −1.596 −0.548 −0.244 EDNRB-Tango 0.401 0.133 −0.198 −0.837 −1.352 −0.897 −0.487 −0.281 ELTD1-Tango −0.120 −0.712 −0.643 −1.052 −1.208 −1.361 −0.959 −0.446 F2R-Tango −0.087 −0.101 −0.345 −1.338 −1.776 −0.944 −0.890 −0.675 F2RL1-Tango −0.302 −0.197 −0.562 −0.872 −1.313 −0.742 −0.712 −0.705 F2RL2-Tango −0.331 −0.558 −0.631 −0.880 −1.372 −0.632 −0.953 −0.694 F2RL3-Tango −0.342 −0.362 −0.445 −1.287 −1.600 −1.028 −0.996 −0.829 FFA1-Tango 0.268 0.276 0.355 −0.944 −0.814 −0.535 −0.804 −0.159 FFA2-Tango 0.471 −0.079 0.241 −1.454 −1.798 −0.495 −0.807 −0.505 FFA3-Tango 0.027 0.035 0.027 −1.150 −1.418 −0.118 −0.597 −0.654 FPR1-Tango 1.057 0.787 0.921 −0.533 −0.448 −1.413 −0.508 0.097 FPR2-Tango 0.802 0.344 0.347 −1.074 −1.541 −0.251 −0.357 −0.090 FPR3-Tango 0.135 −0.090 0.159 −1.208 −1.293 −0.608 −0.780 −0.378 FSHR-Tango −0.208 −0.315 −0.416 −0.656 −0.945 −0.360 −0.563 −0.109 GABBR1-Tango 1.022 0.225 1.355 −0.490 −0.896 −0.350 −0.731 −0.484 GAL1-Tango 0.160 0.261 0.712 −2.705 −2.395 −1.293 −1.235 −0.913 GAL2-Tango −0.853 −0.252 0.320 −1.845 −1.812 −0.840 −1.534 −1.031 GAL3-Tango −0.391 −0.873 −0.671 −1.382 −1.239 −0.739 −1.090 −1.142 GCGR-Tango −0.547 0.210 0.284 −1.359 −1.690 −0.413 −0.523 −0.778 GHRHR-Tango −0.771 0.325 0.288 −1.948 −1.990 −0.313 −1.070 −1.119 GHSR-Tango −0.683 −0.004 0.226 −1.751 −1.710 −1.270 −1.738 −1.525 GIPR-Tango −1.076 −0.035 0.063 −1.493 −2.076 −1.103 −1.336 −0.969 GLP1R-Tango −1.332 −0.631 −0.841 −1.974 −2.572 −0.915 −1.775 −1.157 GLP2R-Tango 0.027 −0.021 −0.134 −2.178 −1.757 −0.143 −0.843 −0.553 GNRHR-Tango −0.053 0.202 0.267 −1.377 −1.555 0.086 −0.374 −0.667 GPBA-Tango 0.677 −0.304 0.019 −1.423 −1.556 0.026 −0.436 −0.792 GPER-Tango 0.218 0.020 0.371 −1.244 −1.737 −0.246 −0.810 −0.501 GPR1-Tango −0.232 0.192 0.057 −1.462 −2.014 −0.343 −1.000 −0.364 GPR101-Tango 0.304 0.287 0.118 −0.840 −0.823 −0.271 −0.324 0.233 GPR110-Tango −0.524 −0.638 −0.576 −0.918 −1.029 −0.573 −1.142 −0.575 GPR111-Tango 0.016 −0.215 −0.135 −0.916 −1.307 −0.504 −1.098 −0.577 GPR113-Tango −0.487 −0.298 −0.368 −1.559 −1.660 −0.751 −0.709 −0.592 GPR114-Tango −0.196 −0.314 −0.244 −0.665 −1.296 −0.312 −0.287 −0.325 GPR115-Tango −0.467 −0.475 −0.507 −0.689 −1.002 −0.499 −0.658 −0.105 GPR116-Tango 0.059 −0.230 −0.149 −1.042 −1.529 −0.534 −1.162 −0.839 GPR119-Tango 0.184 0.249 0.198 −1.059 −1.593 −0.562 −0.448 −0.201 GPR12-Tango 0.150 0.548 0.772 −1.226 −1.697 −0.448 −1.039 −0.350 GPR120-Tango 0.740 0.086 0.605 −0.809 −1.565 0.204 −0.417 −0.681 GPR123-Tango −0.045 0.366 0.165 −0.908 −1.248 −0.088 −0.959 −0.279 GPR124-Tango −0.085 −0.543 −0.388 −1.375 −1.469 −0.173 −1.180 −0.531 GPR125-Tango 0.072 −0.658 −0.806 −0.456 −0.668 −0.340 −0.810 −0.429 GPR126-Tango 0.165 0.097 −0.065 −0.892 −1.408 −0.648 −1.084 −0.853 GPR132-Tango −0.462 −0.837 −0.877 −0.180 −0.792 −0.421 −1.458 −1.065 GPR133-Tango −0.123 −0.326 −0.376 −0.442 −0.907 −0.078 −0.906 −0.667 GPR135-Tango −0.371 0.153 0.170 −0.636 −1.161 −0.523 −0.975 −0.858 GPR141-Tango 0.445 0.124 0.415 −0.748 −0.609 0.247 −1.325 −1.515 GPR142-Tango −0.200 −0.459 −0.476 −1.851 −2.695 −0.483 −1.976 −1.535 GPR143-Tango −0.861 −1.073 −1.370 −2.461 −2.461 −0.306 −1.055 −0.544 GPR144-Tango −0.691 −0.705 −1.196 −1.850 −2.765 −0.662 −1.040 −0.827 GPR146-Tango −0.523 −0.369 −0.528 −1.991 −2.935 0.204 −1.186 −1.298 GPR148-Tango 0.766 −0.127 −0.658 −1.501 −2.446 −0.503 −2.158 −1.771 GPR149-Tango 0.966 −0.902 −0.855 −2.038 −2.809 −0.390 −1.016 0.046 GPR15-Tango −0.347 0.074 0.160 −1.811 −2.223 −0.474 −0.952 −0.310 GPR150-Tango 0.176 0.369 0.170 −0.702 −1.271 −0.145 −0.675 −1.203 GPR151-Tango 0.813 −0.627 −0.478 −1.550 −1.902 −0.227 −0.886 0.178 GPR152-Tango −0.290 −0.200 −0.282 −0.914 −0.998 −0.238 −0.981 −0.503 GPR153-Tango 0.086 −0.099 −0.442 −0.794 −1.035 −0.254 −0.986 −0.240 GPR156-Tango 0.337 0.099 0.247 −0.470 −0.887 0.276 −1.016 −0.377 GPR157-Tango 0.490 −0.175 −0.154 −1.010 −1.803 −0.145 −0.984 −0.662 GPR158-Tango −0.202 −0.093 −0.434 −0.762 −1.225 −0.092 −0.546 −0.461 GPR160-Tango 0.083 −0.271 −0.330 −0.608 −2.772 0.467 −0.349 0.129 GPR161-Tango 0.328 −0.399 −0.345 −0.315 −0.635 −0.016 −0.796 −0.350 GPR162-Tango −0.406 −0.412 −0.483 −0.686 −1.123 −0.496 −0.894 −0.749 GPR17-Tango 0.656 −0.446 −0.516 −1.029 −0.982 −0.074 −0.669 0.121 GPR171-Tango 0.386 −0.279 −0.380 −1.099 −1.377 0.478 −0.814 −0.400 GPR173-Tango 0.176 0.013 −0.290 −1.305 −1.265 0.015 −0.140 −0.906 GPR174-Tango −0.116 −0.144 −0.186 −1.311 −1.390 −0.627 −0.589 −0.623 GPR18-Tango 0.047 −0.228 −0.093 −1.174 −1.368 −0.544 −0.834 −0.866 GPR182-Tango 0.013 −0.584 −0.631 −0.836 −1.007 −0.378 −0.742 −0.459 GPR183-Tango −0.411 −0.089 0.052 −1.105 −1.618 −0.283 −0.635 −0.698 GPR19-Tango −0.493 −0.895 −1.076 −0.936 −1.061 −0.480 −0.966 −0.493 GPR20-Tango 0.605 0.200 0.000 −0.808 −1.514 −0.491 −0.866 −0.463 GPR21-Tango 0.148 0.509 0.133 −1.073 −1.262 −0.170 −0.675 −0.046 GPR22-Tango −0.042 −0.394 −0.405 −0.683 −0.962 0.095 −0.659 −0.087 GPR25-Tango 0.000 −0.197 −0.485 −1.073 −1.515 −0.245 −0.604 −0.518 GPR26-Tango 0.424 0.522 0.636 −1.946 −2.172 −0.959 −1.098 −0.759 GPR27-Tango 0.143 −0.112 −0.028 −1.018 −1.002 0.089 −0.795 −0.206 GPR3-Tango 0.567 0.267 0.408 −1.057 −0.977 −0.733 −0.745 −0.083 GPR31-Tango 0.078 0.048 0.253 −1.704 −2.307 −0.864 −1.477 −0.581 GPR32-Tango −0.259 −0.076 0.110 −1.633 −1.592 −1.093 −0.628 −0.628 GPR34-Tango 0.284 0.239 0.424 −1.580 −1.875 −0.446 −0.945 −0.684 GPR35-Tango −0.080 −0.014 0.005 −1.701 −1.766 −0.832 −1.338 −0.431 GPR37-Tango 0.061 0.011 −0.070 −1.182 −1.446 −0.237 −1.346 −0.701 GPR37L1-Tango −0.110 −0.169 −0.117 −1.336 −1.653 −0.099 −1.182 −0.622 GPR39-Tango −0.260 −0.096 −0.220 −1.003 −1.602 −0.461 −0.958 −0.872 GPR4-Tango −0.313 −0.115 −0.064 −0.620 −0.948 −0.409 −1.073 −0.676 GPR44-Tango −0.166 0.130 0.099 −0.774 −1.367 −0.352 −0.908 −0.677 GPR45-Tango −0.624 −0.119 0.340 −1.462 −1.416 −0.399 −0.650 −0.523 GPR50-Tango 0.218 0.114 −0.085 −1.748 −1.679 0.100 −0.850 0.090 GPR52-Tango 0.459 0.496 0.777 −1.174 −1.399 0.154 −0.930 −0.269 GPR55-Tango 0.440 −0.040 0.097 −1.513 −1.563 0.377 −1.138 −0.307 GPR56-Tango 0.080 0.024 0.097 −1.103 −1.765 −0.431 −1.499 −1.059 GPR6-Tango 0.151 −0.036 0.078 −0.995 −1.362 −1.077 −0.471 −0.012 GPR61-Tango −0.168 −0.024 0.093 −1.012 −1.255 −1.213 −0.838 0.014 GPR62-Tango −0.015 0.133 0.125 −0.727 −1.103 −0.068 −0.643 −0.295 GPR63-Tango −0.130 −0.356 −0.301 −0.335 −0.252 0.041 −0.187 −0.081 GPR64-Tango −0.148 −0.155 −0.040 −0.696 −0.945 −0.189 −0.443 −0.361 GPR65-Tango −0.141 −0.192 −0.100 −0.597 −0.668 −0.172 −0.312 0.002 GPR68-Tango −0.501 −0.421 −0.327 −1.125 −1.380 −0.502 −0.998 −0.899 GPR75-Tango −0.291 −0.155 −0.105 −0.774 −1.200 0.182 −0.362 −0.521 GPR77-Tango −0.473 −0.428 −0.246 −1.730 −2.039 −0.219 −0.945 −0.753 GPR78-Tango 0.141 −0.150 0.025 −1.266 −1.792 −0.527 −0.290 0.124 GPR82-Tango −0.347 −0.123 0.063 −1.640 −1.862 −0.970 −1.356 −0.994 GPR83-Tango −0.634 −0.571 −0.450 −1.015 −1.501 −0.344 −1.228 −0.920 GPR84-Tango −0.090 −0.225 −0.080 −1.164 −1.129 −0.205 −1.297 −0.717 GPR85-Tango 0.128 0.094 0.031 −1.003 −1.468 −0.049 −1.177 −0.516 GPR87-Tango −0.043 0.052 0.076 −0.731 −0.967 −0.137 −0.632 −0.476 GPR88-Tango 0.162 0.111 0.182 −0.869 −1.491 −0.041 −0.564 −0.610 GPR97-Tango 0.056 −0.262 −0.201 −0.796 −1.030 0.037 −0.717 −0.336 GPRC5A-Tango −0.144 −0.193 −0.086 −1.019 −1.205 −0.142 −1.311 −0.826 GPRC5B-Tango −0.155 −0.459 −0.417 −0.787 −0.901 −0.068 −1.143 −0.446 GPRC5C-Tango −0.342 −0.510 −0.714 −0.602 −0.849 0.099 −0.670 −0.109 GPRC5D-Tango −0.146 −0.347 −0.429 −0.811 −1.153 −0.049 −0.894 −0.878 GPRC6A-Tango −0.472 −0.442 −0.757 −0.789 −0.887 0.057 −1.006 −0.419 GRM1-Tango −0.441 −0.284 −0.182 −0.632 −0.863 −0.284 −1.040 −0.688 GRM2-Tango −0.112 −0.415 −0.212 −0.866 −1.157 −0.203 −0.789 −0.762 GRM4-Tango −0.695 −0.616 −0.431 −0.510 −0.749 −0.157 −0.790 −0.334 GRM5-Tango −0.520 −0.351 −0.752 −0.116 −0.060 −0.284 −0.794 −0.220 GRM6-Tango −0.385 −0.285 −0.465 −1.510 −1.404 −0.430 −1.048 −0.567 GRM7-Tango −0.798 −0.585 −0.641 −0.921 −1.287 −0.759 −1.274 −0.217 GRM8-Tango −0.100 −0.511 −0.250 −1.134 −1.561 −0.662 −1.443 −0.696 GRPR-Tango −0.675 0.051 −0.153 −1.309 −2.068 0.161 −1.659 −0.925 HCA1-Tango −0.076 −0.352 0.000 −1.363 −2.254 0.109 −0.758 −1.363 HCA2-Tango 0.444 −0.685 −0.785 −1.040 −1.378 −0.012 −1.003 −0.674 HCA3-Tango 0.100 −0.482 −0.520 −1.355 −1.832 −0.359 −1.042 −0.473 HCTR1-Tango −0.089 0.189 −0.078 −1.228 −2.079 −0.100 −1.138 −1.250 HCTR2-Tango −0.127 0.000 −0.093 −1.217 −1.552 −0.585 −0.545 −0.545 HRH1-Tango 0.535 −0.595 −0.542 −1.425 −1.510 −0.708 −1.297 −0.273 HRH2-Tango 0.634 0.233 0.560 −0.868 −1.317 0.138 −0.820 −0.010 HRH3-Tango 1.883 1.289 1.318 −0.295 −1.260 0.214 −0.053 0.343 HRH4-Tango 1.912 1.740 1.631 −0.096 −0.901 −0.241 −0.638 0.056 HTR1A-Tango 0.583 0.068 0.756 −0.856 −1.621 −0.037 −1.213 −0.512 HTR1B-Tango 0.157 −0.480 −0.366 −0.998 −1.285 −0.524 −1.281 −0.381 HTR1D-Tango 0.419 −0.139 −0.102 −1.232 −1.526 −0.673 −1.235 −0.553 HTR1E-Tango 0.149 −0.282 −0.325 −0.963 −1.146 −0.179 −1.217 −0.403 HTR1F-Tango −0.314 −0.274 −0.136 −1.980 −1.475 −0.480 −1.146 −0.753 HTR2A-Tango −0.145 −0.702 −0.779 −1.055 −2.038 −0.461 −0.950 −0.915 HTR2B-Tango −0.072 −0.698 −0.627 −0.900 −1.067 −0.293 −0.768 −0.405 HTR2C-Tango 0.176 −0.703 −0.389 −1.525 −2.147 −0.547 −0.980 −0.809 HTR4-Tango −0.281 0.347 0.324 −0.426 −1.472 −0.732 −0.682 0.099 HTR5-Tango −0.222 0.186 0.308 −0.934 −1.157 −0.541 −1.462 −1.202 HTR6-Tango 0.098 −0.810 −0.475 −0.169 −1.099 −0.293 −0.860 −0.608 HTR7-Tango 0.417 0.045 0.632 0.791 −0.661 0.079 −1.069 −0.283 KISSPEPTIN-R- 0.196 0.181 1.063 −1.145 −1.525 −0.689 −0.520 −0.094 Tango LHCGR-Tango −0.436 −0.835 −1.011 −1.042 −1.432 −0.589 −1.114 −0.870 LPAR1-Tango 0.207 −0.087 −0.262 −1.110 −1.505 −0.501 −0.690 −0.781 LPAR2-Tango −0.079 0.058 −0.245 −1.204 −1.654 −0.622 −1.117 −0.903 LPAR4-Tango −0.073 −0.098 −0.256 −1.683 −1.545 −1.716 −1.153 −1.153 LPAR5-Tango 0.079 −0.575 −0.318 −1.094 −1.392 −0.906 −1.033 −1.021 LPAR6-Tango 0.087 −0.425 −0.519 −0.555 −1.030 −0.240 −1.185 −0.804 LTB4R-Tango −0.178 −0.520 −0.340 −0.928 −1.509 −0.075 −0.900 −0.949 LTB4R2B-Tango 0.163 −0.984 −0.833 −0.922 −1.384 −0.509 −0.723 −0.519 MAS1-Tango 0.630 0.105 0.181 −1.349 −1.752 −0.575 −0.769 −0.344 MAS1L-Tango 0.422 −0.634 −0.818 −0.841 −1.327 −0.262 −0.669 −0.498 MC1R-Tango 0.589 −0.551 −0.539 −0.837 −1.458 −0.135 −0.472 −0.472 MC2R-Tango 0.069 −0.206 0.000 −1.029 −1.550 −0.247 0.229 0.413 MC3R-Tango 0.175 −0.834 −0.504 −1.022 −1.412 0.115 −0.304 −0.264 MC4R-Tango 0.602 0.000 0.532 −1.484 −1.822 −0.506 −0.541 −1.167 MC5R-Tango 0.869 0.449 0.939 −1.777 −2.139 −1.100 −1.463 −1.087 MCHR1-Tango −0.707 −0.666 −0.880 −1.409 −1.869 −0.167 −1.472 −0.782 MCHR2-Tango −0.094 −0.915 −0.992 −1.230 −1.739 −0.145 −0.695 −1.100 MLNR-Tango 0.445 −0.077 −0.216 −0.914 −1.243 0.052 −0.615 −0.597 MRGPRD-Tango −0.164 −0.689 −0.694 −1.125 −1.309 −0.229 −1.120 −0.307 MRGPRE-Tango 0.214 −0.175 0.036 −0.605 −0.987 0.330 −0.462 −0.531 MRGPRF-Tango 0.079 0.293 0.326 −0.531 −1.094 0.276 −0.608 −0.397 MRGPRG-Tango −0.008 −0.288 −0.170 −0.035 −0.111 0.249 −0.482 −0.142 MRGPRX1-Tango −0.037 −0.473 0.108 −0.836 −1.283 −0.191 −1.563 −1.288 MRGPRX2-Tango −0.101 −0.660 −0.582 −1.647 −1.448 0.049 −0.772 −0.461 MRGPRX3-Tango −0.106 −0.426 −0.300 −1.371 −1.991 −0.163 −1.125 −1.033 MRGPRX4-Tango −0.654 −0.720 −1.138 −2.281 −2.738 −1.216 −2.033 −1.854 MTNR1A-Tango 0.064 0.263 0.354 −2.107 −2.164 −0.425 −2.050 −1.102 MTNR1B-Tango 0.103 −0.186 0.639 −2.001 −2.390 −0.770 −1.166 −0.634 NMBR-Tango −0.603 −0.365 −0.171 −1.257 −1.579 0.249 −0.054 −0.143 NMUR1-Tango −0.888 0.000 −0.160 −1.476 −1.940 −0.691 −0.930 −1.507 NMUR2-Tango −0.881 −0.835 −0.629 −1.497 −1.364 −0.295 −2.108 −1.966 NPBW1-Tango 0.597 0.437 0.532 −1.657 −1.038 −0.228 −0.675 −0.420 NPBW2-Tango 0.541 0.000 0.424 −1.186 −1.067 −0.637 −1.439 −0.194 NPFF1-Tango 0.439 0.266 0.200 −0.998 −1.131 0.519 −0.611 −0.815 NPFF2-Tango −0.519 −0.274 −0.497 −1.434 −1.454 −0.212 −0.990 −0.301 NPS-Tango −0.452 −0.535 −0.679 −1.234 −1.589 −0.232 −1.741 −0.744 NPY1R-Tango −0.126 −0.750 −0.474 −1.237 −1.381 −0.518 −1.239 0.097 NPY2R-Tango −0.272 −0.580 −0.351 −0.807 −0.990 0.418 −0.856 −0.159 NPY4R-Tango −0.142 0.188 0.249 −0.926 −1.348 −0.110 −1.703 −0.509 NPY5R-Tango −0.051 −0.446 −0.312 −1.109 −1.596 0.262 −0.656 −0.613 NTSR1-Tango 0.005 −0.218 −0.200 −1.214 −1.198 −0.654 −0.144 −0.083 NTSR2-Tango 0.196 −0.324 −0.462 −1.371 −1.830 −0.711 −0.504 −0.644 OPN3-Tango 0.028 0.120 0.000 −1.182 −1.614 −0.229 −0.065 −0.411 OPN5-Tango −0.171 −0.679 −0.525 −1.580 −1.939 −1.036 −0.534 −0.111 OPRD1-Tango −0.201 −0.283 −0.465 −1.560 −2.095 −0.689 −1.169 −1.068 OPRK1-Tango 0.509 −0.291 −0.327 −0.828 −0.933 −0.348 −0.935 −0.545 OPRL1-Tango −0.048 0.089 −0.411 −0.870 −1.173 −0.193 −0.892 −0.922 OPRM1-Tango −0.150 −0.374 0.070 −1.201 −1.544 −0.518 −0.367 −0.647 OXER1-Tango −0.084 −0.470 −0.822 −0.801 −1.156 −0.061 −0.358 −0.294 OXGR1-Tango −0.469 −0.645 −0.510 −2.202 −2.582 −0.912 −1.250 −1.117 OXTR-Tango 0.281 −0.117 −0.193 −0.960 −1.573 −0.194 −0.218 −0.067 P2RY1-Tango 0.185 −0.914 −1.038 −1.254 −1.388 −0.614 −1.108 −0.650 P2RY10-Tango 0.258 −0.177 −0.562 −0.885 −1.629 −0.401 −0.927 −0.705 P2RY11-Tango −0.174 −0.835 −0.365 −1.106 −1.701 −1.394 −0.669 −0.431 P2RY12-Tango −0.240 −0.111 −0.496 −1.404 −1.840 −0.747 −0.550 −0.586 P2RY13-Tango 0.071 −0.255 −0.365 −1.730 −1.699 −0.516 −1.253 −0.821 P2RY14-Tango 0.304 −0.538 −0.612 −1.201 −1.635 −0.341 −1.060 −0.305 P2RY2-Tango −0.112 −0.648 −0.685 −1.684 −1.949 −0.840 −1.104 −0.702 P2RY4-Tango −0.322 −0.543 −0.744 −1.806 −2.051 −1.043 −1.602 −1.299 P2RY6-Tango 0.074 −0.607 −0.634 −1.664 −2.068 −0.296 −1.023 −0.776 P2RY8-Tango −0.148 −0.397 −0.637 −1.313 −1.845 −0.527 −1.476 −0.815 PK1-Tango 0.168 −0.246 −0.637 −1.323 −1.908 −0.527 −0.788 −0.919 PK2-Tango −0.013 0.255 −0.181 −1.328 −1.574 −0.079 −0.444 −0.444 PRRP-Tango 0.100 −0.334 −0.202 −1.525 −1.978 −0.286 −0.442 −0.323 PTAFR-Tango 0.029 −0.840 −0.902 −0.986 −1.366 −0.258 −0.586 0.079 PTGDR-Tango −0.133 −0.580 −0.822 −1.355 −1.777 −0.646 −0.674 −0.958 PTGER1-Tango −0.021 0.293 0.762 −0.755 −1.296 −0.085 −0.613 −0.756 PTGER2-Tango 0.356 0.471 0.898 −1.303 −1.389 −0.074 −0.564 −0.328 PTGER3-Tango −0.010 −0.409 −0.125 −1.164 −1.462 −0.057 −0.825 −0.226 PTGER4-Tango 0.026 −0.343 −0.051 −1.074 −1.699 −0.038 −0.796 −0.499 PTGFR-Tango −0.136 −0.580 −0.032 −1.795 −2.596 −0.592 −1.942 −0.615 PTGIR-Tango −0.027 −0.411 −1.394 −1.215 −1.412 −1.156 −1.291 −0.493 PTH1R-Tango −0.436 −0.401 −0.457 −1.095 −2.021 −0.094 −1.147 −0.867 PTH2R-Tango −0.425 −0.678 −0.696 −0.921 −1.248 −0.049 −1.130 −0.201 QRFP-Tango −0.474 −0.142 −0.206 −1.147 −1.787 −0.091 −0.971 −0.601 RXFP1-Tango −0.588 −0.476 −0.792 −0.573 −0.866 −0.636 −0.697 −0.400 RXFP2-Tango −0.926 −1.037 −1.199 −1.230 −1.536 −0.460 −1.215 −1.015 RXFP3-Tango −0.436 −0.525 −0.922 −0.943 −1.548 −0.121 −1.285 −1.442 RXFP4-Tango −0.495 −0.437 −0.505 −1.070 −1.461 −0.284 −0.465 −0.355 S1PR1-Tango −0.455 −0.038 0.215 −0.812 −1.141 −0.171 0.592 1.055 S1PR2-Tango −0.578 −0.465 −0.927 −1.865 −2.063 −0.718 0.023 0.248 S1PR3-Tango −0.529 −0.160 0.080 −1.458 −1.597 0.032 −0.389 −0.155 S1PR4-Tango −0.446 −0.483 −0.334 −2.274 −2.361 −0.383 −0.817 −0.524 S1PR5-Tango −0.114 0.125 −0.136 −1.222 −1.897 0.284 −1.350 −0.216 SCTR-Tango 0.132 0.620 1.201 −1.684 −1.808 −0.224 0.154 −0.723 SSTR1-Tango −0.690 −0.068 −0.068 −1.867 −2.063 −0.490 −1.252 −0.545 SSTR2-Tango −0.729 0.322 −0.333 −1.075 −1.403 −0.228 0.140 0.000 SSTR3-Tango 0.000 −0.697 0.243 −1.029 −1.305 −0.021 −0.718 0.082 SSTR4-Tango 0.287 −0.468 0.127 −1.688 −1.442 0.428 −1.148 −0.392 SSTR5-Tango 1.129 0.660 0.399 −0.773 −0.985 1.286 −0.268 −0.284 SUCNR1-Tango 3.428 2.283 2.551 2.860 2.371 3.911 1.794 2.560 TA1-Tango −0.359 −0.509 −0.614 −0.632 −1.625 0.602 −0.653 −0.289 TAAR2-Tango −0.050 −0.764 −0.856 −0.571 −1.527 −0.109 −0.379 −0.657 TAAR5-Tango 0.305 −0.491 −0.676 −1.021 −1.668 −0.057 −1.000 −0.843 TAAR6-Tango −0.480 −0.750 −0.866 −0.734 −0.894 0.032 −0.962 −0.373 TAAR8-Tango −0.306 −0.573 −0.447 −0.792 −1.145 0.030 −0.623 −0.336 TAAR9-Tango −0.210 −0.261 −0.657 −0.713 −1.020 −0.231 −1.152 −0.463 TACR1-Tango −0.197 −0.666 −0.471 −0.841 −1.058 −0.068 −0.790 0.021 TACR2-Tango −0.086 −0.246 −0.360 −0.831 −1.397 0.084 −0.416 −0.409 TACR3-Tango −0.407 −0.764 −0.723 −1.293 −1.867 0.139 −0.577 −0.238 TBXA2R-Tango −0.184 −0.333 −0.315 −0.917 −1.925 −0.051 −0.451 −0.567 TSHR-Tango −0.745 −1.244 −1.178 −1.271 −1.865 −0.428 −1.503 −0.845 UTS2R-Tango −0.054 0.176 −0.503 −1.025 −1.816 −0.101 −0.858 −0.629 VIPR1-Tango −0.060 −0.550 −0.266 −1.823 −1.948 −0.242 −1.677 −0.842 VIPR2-Tango 1.222 −0.305 −0.405 −1.361 −1.604 0.300 0.270 1.372 (Enterobac- (Enterobac- (Enterobac- (Enterobac- (Enterobac- (Enterobac- (Enterobac- (Enterobac- PRESTO-Tango teria)UC/ teria)UC/ teria)UC/ teria)UC/ teria)UC/ teria)UC/ teria)UC/ teria)UC/ with GMM UC-6# UC-7# UC-8# UC-9# UC-10# UC-11# UC-12# UC-13# ADCYAP1R1- −0.406 −0.401 −0.354 0.212 −0.306 0.159 0.457 0.389 Tango ADORA1-Tango −1.118 −0.770 −0.603 0.241 −1.284 −0.643 −0.026 −0.028 ADORA2A-Tango 0.021 −0.458 −0.154 0.434 −0.651 −0.331 0.089 0.327 ADORA2B-Tango −0.358 −0.248 −0.156 −0.133 −0.372 −0.534 −0.223 0.022 ADORA3-Tango −0.130 0.139 0.241 0.508 −0.303 0.343 −0.064 0.537 ADRA1A-Tango −0.188 −0.006 0.209 0.155 −0.409 −0.451 −0.284 0.404 ADRA1B-Tango −0.119 −0.095 0.078 0.262 −0.467 0.154 −0.808 −0.129 ADRA1D-Tango −0.178 −0.082 0.019 0.104 −0.467 −0.425 −0.224 0.411 ADRA2A-Tango −0.316 −0.220 −0.060 −0.038 −1.032 −0.058 0.181 0.405 ADRA2B-Tango −1.089 −0.916 −0.887 −0.360 −1.423 −1.044 −0.501 −0.906 ADRA2C-Tango −0.830 −0.631 −0.563 0.062 −0.600 −0.288 0.282 0.631 ADRB1-Tango −0.224 −0.325 0.474 −0.316 0.202 0.574 0.032 0.468 ADRB2-Tango −0.974 −0.629 −0.669 −0.360 −0.044 −0.542 0.455 0.133 ADRB3-Tango −0.330 −0.690 −0.792 0.035 0.122 −0.087 −0.397 −0.549 AGTR1-Tango −0.040 −0.461 −0.227 0.328 −0.199 −0.430 0.453 0.616 AGTR2-Tango −0.500 0.010 −0.222 0.207 −0.369 0.151 0.493 0.730 APJ-Tango −0.659 −0.472 −0.023 −0.014 −1.107 −1.162 0.232 −0.034 AVPR1A-Tango −0.615 −0.389 −0.092 0.021 −0.189 −0.962 −0.395 −0.127 AVPR1B-Tango −0.280 −0.233 −0.204 0.100 −0.265 −0.424 −0.464 −0.374 AVPR2-Tango −1.137 −0.847 −0.579 0.053 −0.985 −0.834 0.232 0.359 BB3-Tango −0.451 −0.311 −0.258 0.155 −0.298 −0.213 −0.581 −0.549 BDKBR1-Tango −0.081 0.236 −0.210 0.150 −0.106 −0.672 −0.559 −0.613 BDKBR2-Tango 0.271 0.161 0.291 0.209 0.020 0.368 0.052 0.364 C3AR1-Tango −0.384 −0.407 −0.119 0.174 −0.149 −0.266 −0.467 −0.105 C5A-Tango 0.187 0.086 0.548 0.458 −0.047 −0.027 −0.530 0.308 CALCRb-Tango −0.107 0.000 0.068 0.293 −0.060 −0.705 −0.413 −0.474 CALCRL-Tango −0.251 −0.087 0.027 0.355 −0.120 −0.389 −0.468 −0.047 CASR-Tango −0.327 −0.389 −0.147 0.227 0.297 −0.135 −1.413 −1.094 CCKAR-Tango 0.064 0.258 0.271 0.262 −0.067 0.207 −0.588 0.094 CCKBR-Tango −0.336 −0.053 −0.234 −0.023 −0.425 −0.851 −0.448 −0.739 CCR10-Tango −0.628 −0.693 −0.363 −0.315 −0.369 −0.632 −0.384 −0.299 CCR2-Tango −0.284 0.038 −0.059 −0.013 −0.210 −0.575 −0.993 −0.748 CCR3-Tango 0.026 0.451 0.073 0.364 −0.810 −0.237 0.266 0.604 CCR4-Tango −0.429 0.130 −0.188 0.335 −0.341 −0.179 −0.172 0.142 CCR5-Tango 0.203 0.366 0.479 0.131 0.297 0.203 0.371 0.457 CCR6-Tango 0.148 0.469 0.469 0.093 0.203 0.297 0.489 0.616 CCR7-Tango −0.470 −0.328 −0.101 0.175 −0.221 −0.160 −0.094 −0.330 CCR8-Tango −0.487 −0.265 −0.302 −0.355 −0.642 −0.705 0.181 0.373 CCRL2-Tango −0.135 0.108 0.218 0.575 −0.547 −0.055 0.415 0.565 CD97-Tango −0.302 −0.435 −0.022 0.627 −0.514 −0.354 −0.687 −0.994 CHRM1-Tango −0.537 −0.963 −0.413 −0.118 −0.876 −0.589 0.136 0.463 CHRM2-Tango −0.916 −0.880 −0.827 0.026 −0.852 −0.966 −0.452 −0.835 CHRM3-Tango −0.990 −0.754 −0.826 0.394 −0.483 −1.493 0.249 0.415 CHRM4-Tango −0.415 −0.011 −0.205 0.062 0.070 −0.065 −0.082 0.216 CHRM5-Tango −0.399 −0.238 −0.263 0.180 0.350 0.460 −0.133 0.154 CMKLR1-Tango −0.220 −0.212 0.000 0.159 −0.535 −0.493 −0.228 −0.170 CNR1-Tango −0.477 −0.422 −0.416 0.280 −0.107 −1.473 −0.218 −0.046 CNR2-Tango −0.288 −0.201 −0.183 0.311 −0.678 −0.704 0.055 0.601 CRHR1-Tango −0.064 0.455 0.037 0.225 0.057 −0.336 0.525 0.000 CRHR2-Tango −0.702 −0.348 0.088 0.040 −0.195 −0.353 −0.345 1.160 CX3C1-Tango 0.119 −0.286 0.055 −0.045 −0.186 −0.604 −0.445 −0.245 CXCR1-Tango 0.371 −0.040 −0.089 −0.160 0.504 0.327 −0.129 1.017 CXCR2-Tango 0.670 1.019 0.660 −0.052 0.625 0.867 0.859 1.344 CXCR3-Tango −0.289 −0.077 −0.485 0.104 −0.394 −0.441 0.404 0.850 CXCR4-Tango −0.020 −0.146 −0.052 0.604 −0.017 0.158 0.661 1.264 CXCR5-Tango 0.139 −0.143 0.066 0.375 −0.130 −0.196 0.048 0.610 CXCR6-Tango −0.705 −0.665 −0.120 −0.413 0.500 −0.445 1.415 1.595 CXCR7-Tango −0.823 −0.740 −0.565 0.057 −0.720 −0.828 0.564 0.584 CYSLTR1-Tango −0.073 0.134 −0.023 0.345 0.019 −0.037 0.575 0.951 CYSLTR2-Tango 0.021 0.096 −0.473 0.031 0.211 −0.405 0.313 0.960 DRD1-Tango −1.165 −1.047 −0.986 −0.016 0.295 −0.819 0.928 1.006 DRD2-Tango −0.391 −0.640 −1.126 0.299 −0.628 −1.011 0.084 0.318 DRD3-Tango −0.100 −0.728 −1.451 0.447 0.594 −0.246 0.565 0.437 DRD4-Tango −0.792 −0.642 −0.520 0.787 0.234 0.234 −0.433 0.455 DRD5-Tango −0.478 −0.392 −0.273 −0.284 −0.040 −0.682 0.029 0.318 EDNRA-Tango −0.254 −0.019 −0.031 0.369 −0.218 −0.301 −0.060 0.226 EDNRB-Tango −0.214 −0.277 −0.218 0.191 −0.278 −0.684 −0.138 −0.081 ELTD1-Tango −0.437 −0.485 −0.432 0.308 −0.561 −1.097 −0.604 −0.844 F2R-Tango −0.149 −0.086 −0.252 0.179 −0.226 −0.865 −0.222 −0.033 F2RL1-Tango −0.335 −0.211 −0.366 0.100 0.037 −0.449 −0.336 −0.090 F2RL2-Tango −0.609 −0.448 −0.405 0.377 0.050 −0.071 −0.153 −0.282 F2RL3-Tango −0.280 −0.416 −0.379 0.330 −0.184 −0.598 0.003 −0.005 FFA1-Tango −0.505 −0.206 −0.230 0.670 −0.036 0.028 −0.029 −0.117 FFA2-Tango −0.729 −0.735 −0.782 0.413 −0.417 −0.644 −0.050 0.013 FFA3-Tango −0.893 −0.585 −0.379 0.248 0.297 −0.257 0.020 0.351 FPR1-Tango −0.061 −0.005 0.224 0.446 0.766 0.030 0.975 0.210 FPR2-Tango −0.264 −0.120 −0.109 0.096 0.677 −1.000 0.313 0.104 FPR3-Tango −0.857 −0.442 −0.323 0.291 −0.394 −0.410 0.298 0.170 FSHR-Tango −0.650 −0.432 −0.267 0.443 −0.196 −0.444 −0.601 −0.480 GABBR1-Tango −0.097 0.038 −0.369 −0.062 0.026 −0.572 0.123 −0.260 GAL1-Tango −0.940 −0.330 −0.940 −0.361 −1.117 −1.296 −0.470 −0.698 GAL2-Tango −0.124 0.267 −0.079 0.515 −1.094 −0.982 0.791 1.322 GAL3-Tango −0.444 −0.415 −0.263 −0.198 −0.501 −0.968 −0.962 0.026 GCGR-Tango −0.366 −0.240 −0.289 0.194 −0.285 −0.876 −0.301 0.125 GHRHR-Tango −0.467 −0.115 −0.284 0.241 −0.131 −0.942 −0.210 −0.079 GHSR-Tango −0.977 −0.721 −0.458 0.429 −0.650 −0.622 0.169 0.532 GIPR-Tango −0.677 −0.544 −0.299 −0.054 −0.342 −1.245 0.000 0.000 GLP1R-Tango −0.753 −0.303 −0.354 0.276 −0.970 −1.348 −0.571 −0.348 GLP2R-Tango −0.178 0.309 0.309 0.005 −0.422 −0.579 0.261 0.614 GNRHR-Tango −0.592 −0.235 −0.393 −0.293 −0.542 −1.044 0.000 −0.060 GPBA-Tango −0.990 −0.527 −0.379 0.371 −0.510 −0.220 0.945 0.803 GPER-Tango −0.034 0.165 0.229 0.343 −0.057 −0.468 −0.312 0.330 GPR1-Tango −0.543 −0.366 −0.169 0.141 −0.343 −0.265 0.050 −0.089 GPR101-Tango −0.058 0.036 −0.082 0.261 0.059 0.065 0.359 0.291 GPR110-Tango −0.312 −0.217 −0.042 −0.018 −0.432 −0.408 −0.538 −0.460 GPR111-Tango −0.195 −0.120 −0.048 −0.201 −0.135 −0.579 −0.377 −0.467 GPR113-Tango −0.963 −0.582 −0.510 0.089 −0.331 −0.820 −0.441 −0.153 GPR114-Tango −0.281 −0.322 −0.400 −0.204 −0.274 −0.938 −0.053 −0.421 GPR115-Tango −0.227 −0.445 −0.497 0.050 −0.519 −0.272 −0.631 −0.493 GPR116-Tango −0.221 −0.176 −0.237 0.101 −0.835 −0.778 −0.609 −0.771 GPR119-Tango −0.402 −0.101 −0.268 0.094 −0.509 −0.687 0.000 0.188 GPR12-Tango −0.335 0.084 −0.089 0.376 0.149 −0.098 0.349 0.518 GPR120-Tango −0.109 0.072 −0.296 0.015 −0.477 −0.500 −0.355 −0.105 GPR123-Tango −0.319 −0.115 0.231 0.291 −0.113 0.015 0.202 0.778 GPR124-Tango −0.292 −0.664 −0.623 0.099 −0.130 −0.423 −0.335 0.133 GPR125-Tango −0.500 −0.562 −0.300 −0.209 0.256 0.076 −0.147 0.109 GPR126-Tango −0.748 −0.489 −0.689 −0.088 −0.431 −0.924 −0.548 −0.586 GPR132-Tango −1.342 −0.897 −0.878 0.397 −1.119 −1.261 −0.601 −0.372 GPR133-Tango −0.644 −0.629 −0.634 0.281 −0.307 −0.436 −0.527 −0.181 GPR135-Tango −0.473 −0.562 −0.409 −0.185 −0.019 −0.727 −0.040 0.100 GPR141-Tango −0.023 −0.301 −0.414 0.635 −0.093 −0.422 0.286 0.314 GPR142-Tango −0.492 −0.593 −0.501 0.125 −0.657 −0.785 0.068 0.575 GPR143-Tango −0.736 −0.579 −0.390 0.400 −0.694 −0.763 −0.605 −0.651 GPR144-Tango −0.927 −0.358 −0.449 −0.029 −0.846 −1.968 −0.840 −0.523 GPR146-Tango 0.154 −0.395 0.745 0.531 0.766 −0.368 −0.104 0.000 GPR148-Tango 0.341 0.968 0.968 −0.066 0.282 0.000 1.026 −0.284 GPR149-Tango −0.260 0.038 −0.135 0.144 0.506 0.126 −0.317 0.816 GPR15-Tango −0.243 −0.170 −0.132 0.361 −0.358 −0.554 0.313 0.718 GPR150-Tango 0.437 0.102 0.357 −0.160 0.000 −0.463 0.112 0.376 GPR151-Tango −0.414 −0.165 −0.526 0.544 0.195 0.054 −0.329 −0.302 GPR152-Tango −0.612 −0.392 −0.574 −0.077 −0.384 −0.908 −0.607 −0.398 GPR153-Tango −0.370 −0.434 −0.446 0.087 0.063 0.147 0.016 0.301 GPR156-Tango −0.570 −0.536 −0.678 −0.232 −0.793 −1.106 −0.002 0.023 GPR157-Tango −0.423 −0.528 −0.331 0.215 −0.169 −0.956 0.058 0.150 GPR158-Tango 0.029 −0.114 −0.114 0.065 −0.178 −0.934 −0.672 −0.526 GPR160-Tango −0.315 −0.390 −0.315 0.247 0.041 −0.219 −0.158 0.218 GPR161-Tango −0.356 −0.332 −0.237 0.176 0.441 0.115 0.227 0.618 GPR162-Tango −0.601 −0.386 −0.714 −0.092 −0.098 −0.431 −0.287 0.203 GPR17-Tango −0.854 −0.774 −0.750 0.463 0.363 0.393 0.446 0.603 GPR171-Tango −0.631 −0.503 −0.592 0.190 −0.341 −0.547 0.200 0.411 GPR173-Tango −0.614 −0.371 −0.494 −0.174 0.326 −0.532 0.163 0.186 GPR174-Tango −0.043 −0.306 −0.360 0.526 −0.211 −0.495 0.265 0.688 GPR18-Tango −0.469 −0.196 −0.405 0.316 −0.218 −0.672 0.278 0.404 GPR182-Tango −0.692 −0.671 −0.716 0.602 0.107 0.075 0.106 0.241 GPR183-Tango −0.344 −0.279 −0.661 0.507 −0.165 −0.591 0.204 0.000 GPR19-Tango −0.774 −0.823 −0.749 0.652 0.132 0.079 −0.488 −0.351 GPR20-Tango −0.048 0.423 0.000 1.322 1.036 −0.368 0.104 0.350 GPR21-Tango 0.105 0.022 −0.344 0.629 0.033 −0.613 0.000 0.118 GPR22-Tango 0.016 0.236 0.125 0.293 0.082 −0.118 0.642 0.660 GPR25-Tango −0.299 0.226 0.132 0.070 0.077 −0.728 0.165 0.301 GPR26-Tango −0.431 −0.259 −0.243 0.332 0.627 0.031 0.054 0.216 GPR27-Tango 0.221 0.023 −0.428 0.214 0.214 −0.534 0.803 0.595 GPR3-Tango −0.762 −0.852 −0.812 0.559 −0.179 −0.185 0.620 0.668 GPR31-Tango −0.842 −1.057 −0.998 0.750 −0.403 −0.218 0.154 0.503 GPR32-Tango −0.019 −0.307 −0.162 0.428 −0.379 −0.799 0.293 0.171 GPR34-Tango −0.549 −0.288 −0.350 0.288 −0.669 −0.650 0.172 0.372 GPR35-Tango −1.176 −0.564 −0.596 0.396 −0.783 −0.480 0.225 0.168 GPR37-Tango −0.526 −0.068 0.119 0.482 −0.274 0.173 0.539 0.839 GPR37L1-Tango −0.259 −0.092 −0.087 0.106 0.205 0.063 −0.329 −0.256 GPR39-Tango −0.259 −0.129 −0.185 −0.035 −0.304 −0.705 −0.446 −0.570 GPR4-Tango 0.100 0.065 0.083 0.372 −0.135 0.138 0.389 0.606 GPR44-Tango −0.385 −0.345 −0.350 0.023 −0.090 −0.566 0.050 −0.057 GPR45-Tango −0.409 −0.515 −0.655 0.108 −0.411 −0.293 0.385 0.145 GPR50-Tango 0.516 0.436 0.206 0.475 0.160 0.365 0.453 1.368 GPR52-Tango 0.216 −0.020 0.233 −0.211 0.108 0.175 0.898 0.502 GPR55-Tango −0.410 −0.164 0.009 0.260 −0.560 −0.117 0.490 0.358 GPR56-Tango −0.366 0.039 −0.026 0.352 −0.432 −0.192 −0.369 −0.006 GPR6-Tango −0.052 −0.058 0.357 0.540 0.037 0.444 0.626 0.915 GPR61-Tango −0.357 −0.153 −0.185 0.250 0.025 0.160 0.397 0.381 GPR62-Tango −0.324 0.013 0.144 0.303 −0.269 −0.112 −0.379 0.140 GPR63-Tango −0.246 0.188 −0.213 −0.252 −0.156 −0.123 −0.486 −0.224 GPR64-Tango −0.124 −0.091 −0.177 −0.293 −0.031 −0.247 −0.143 0.196 GPR65-Tango 0.325 0.483 0.198 −0.035 −0.044 0.153 0.048 0.251 GPR68-Tango −0.111 −0.121 −0.139 −0.055 −0.403 −0.545 −0.181 0.149 GPR75-Tango 0.179 0.510 0.619 0.219 0.069 −0.003 0.586 1.155 GPR77-Tango −0.561 −0.245 −0.221 −0.058 −0.495 −0.547 −0.052 0.315 GPR78-Tango −0.285 0.189 0.224 0.208 0.318 −0.088 −0.008 0.378 GPR82-Tango −1.345 −0.674 −0.334 −0.066 −0.491 −0.331 0.098 1.013 GPR83-Tango −0.115 −0.004 0.181 0.239 −0.326 −0.423 0.009 0.428 GPR84-Tango −0.037 0.157 0.248 0.215 −0.633 −0.459 0.369 0.831 GPR85-Tango 0.210 0.063 0.179 0.110 −0.605 −0.513 0.183 0.454 GPR87-Tango −0.138 0.404 0.472 0.230 −0.218 0.277 0.612 1.513 GPR88-Tango −0.029 0.178 −0.022 0.090 −0.370 −0.417 0.524 0.631 GPR97-Tango −0.286 −0.025 −0.025 0.335 −0.347 −0.655 0.272 0.305 GPRC5A-Tango −0.538 −0.157 −0.118 0.470 −0.417 −0.188 0.251 0.480 GPRC5B-Tango −0.339 −0.085 0.131 0.516 −0.513 −0.239 0.054 0.645 GPRC5C-Tango 0.240 0.596 0.584 0.523 −0.317 −0.227 0.597 0.553 GPRC5D-Tango −0.054 0.007 0.028 −0.040 −0.077 −0.667 −0.209 −0.110 GPRC6A-Tango 0.187 0.356 0.637 −0.069 −0.579 −0.813 −0.112 0.014 GRM1-Tango 0.070 −0.038 0.252 −0.202 0.036 −0.896 −0.071 −0.450 GRM2-Tango 0.000 −0.052 0.043 −0.125 −0.386 −1.006 −0.226 −0.116 GRM4-Tango 0.065 0.307 0.408 0.598 −0.115 0.176 −0.372 0.125 GRM5-Tango 0.251 0.259 0.937 −0.349 0.198 −0.602 0.061 −0.107 GRM6-Tango −0.339 −0.124 −0.035 −0.478 0.218 −0.556 −0.159 0.125 GRM7-Tango 0.367 0.312 0.230 −0.476 −0.775 −1.329 −0.548 −0.443 GRM8-Tango −0.359 −0.180 −0.171 −0.154 −0.335 −0.865 −0.117 −0.650 GRPR-Tango −0.075 0.226 0.817 0.313 −0.232 −0.614 0.745 1.136 HCA1-Tango −0.111 −0.023 −0.055 0.211 −0.122 −0.457 0.202 0.550 HCA2-Tango 0.247 0.671 0.752 0.925 −0.553 −0.400 1.186 0.741 HCA3-Tango −0.793 −0.381 −0.165 0.597 −0.643 −1.040 0.291 0.437 HCTR1-Tango −0.376 −0.216 −0.136 0.840 0.577 −0.248 0.719 0.322 HCTR2-Tango −1.350 −0.350 −0.154 −0.248 0.545 0.282 −0.387 −0.133 HRH1-Tango −0.526 −0.255 −0.467 0.201 −0.138 0.307 0.708 0.845 HRH2-Tango −0.087 −0.365 1.440 0.145 −0.056 −0.081 0.276 0.343 HRH3-Tango 0.251 0.452 0.820 −0.568 0.162 0.338 0.817 1.741 HRH4-Tango 0.650 1.103 0.871 0.081 0.625 0.436 0.511 1.860 HTR1A-Tango 0.198 0.406 0.221 0.278 0.041 0.556 0.395 1.002 HTR1B-Tango 0.022 0.243 0.056 0.398 −0.201 0.526 0.731 1.170 HTR1D-Tango −0.381 −0.088 −0.106 0.249 −0.474 0.263 0.472 0.824 HTR1E-Tango −0.132 0.111 0.138 0.396 −0.006 0.797 0.397 1.073 HTR1F-Tango −1.334 −0.763 −0.212 0.691 −0.365 0.344 0.498 0.741 HTR2A-Tango −0.183 0.052 0.241 −0.118 −0.248 −0.396 0.443 0.419 HTR2B-Tango −0.149 0.127 0.269 0.336 −0.243 −0.420 0.336 0.359 HTR2C-Tango −0.262 −0.220 −0.266 −0.087 −0.065 −0.681 −0.402 −0.208 HTR4-Tango 0.100 −0.071 0.090 0.286 0.306 0.194 0.579 0.513 HTR5-Tango −1.302 −0.684 −0.146 0.528 0.108 0.121 0.991 1.311 HTR6-Tango 0.120 0.187 0.413 0.399 0.053 −0.149 0.159 0.495 HTR7-Tango 0.312 0.625 0.694 0.400 0.367 0.439 0.304 0.604 KISSPEPTIN-R- −0.092 0.127 −0.092 −0.642 0.326 −0.631 0.122 −0.065 Tango LHCGR-Tango −0.457 −0.357 −0.006 0.303 −0.489 −0.284 −0.379 −0.038 LPAR1-Tango −0.917 −0.262 0.078 0.410 −0.140 −0.230 0.306 0.602 LPAR2-Tango −1.047 −0.624 −0.223 0.035 −0.312 −0.753 −0.120 −0.015 LPAR4-Tango −1.946 −1.012 −0.545 0.215 −0.173 −0.296 0.100 0.360 LPAR5-Tango −0.656 −0.486 −0.392 −0.030 −0.152 −0.816 −0.048 −0.012 LPAR6-Tango −0.386 −0.086 0.049 0.428 0.460 0.610 0.720 0.992 LTB4R-Tango −0.468 −0.089 −0.295 0.036 0.246 −0.097 0.257 0.635 LTB4R2B-Tango 0.182 0.222 0.178 0.360 −0.132 0.030 0.244 0.328 MAS1-Tango −0.191 0.246 0.108 −0.037 −0.242 −0.008 0.089 0.162 MAS1L-Tango 0.001 0.161 0.311 −0.123 0.050 −0.009 −0.273 −0.544 MC1R-Tango −0.162 0.252 0.047 −0.010 −0.025 −0.568 0.102 0.065 MC2R-Tango 0.123 −0.200 0.096 0.242 0.171 −0.163 0.520 0.628 MC3R-Tango −0.100 −0.117 0.155 0.078 −0.234 −0.098 −0.122 0.049 MC4R-Tango 0.000 0.405 0.381 0.681 −0.049 −0.429 0.705 0.603 MC5R-Tango −0.130 0.085 0.442 0.509 0.273 0.031 0.390 0.435 MCHR1-Tango −0.709 −0.691 −0.670 −1.153 0.153 −0.124 −0.441 −0.094 MCHR2-Tango −0.209 −0.518 0.157 −0.246 0.235 −0.178 0.026 −0.418 MLNR-Tango −0.310 −0.023 −0.310 −0.067 −0.215 −0.252 0.321 0.117 MRGPRD-Tango −0.471 −0.170 −0.410 0.068 −0.169 0.071 0.033 0.244 MRGPRE-Tango −0.213 −0.106 −0.332 −0.169 −0.040 −0.159 0.325 0.331 MRGPRF-Tango 0.035 0.327 0.295 0.312 −0.132 −0.012 0.319 0.412 MRGPRG-Tango 0.183 0.058 0.038 −0.483 0.046 0.238 −0.271 −0.203 MRGPRX1-Tango −0.459 −0.527 −0.599 −0.555 0.215 −0.046 −0.100 0.229 MRGPRX2-Tango −0.518 −0.205 0.242 −0.118 −0.012 −0.156 0.355 0.480 MRGPRX3-Tango −0.366 −0.386 0.165 −0.167 −0.078 −1.367 0.364 −0.079 MRGPRX4-Tango −1.078 −0.797 −0.588 −0.459 −1.064 −1.628 −0.149 −0.053 MTNR1A-Tango −0.497 −0.014 −0.023 0.171 −0.714 −0.483 0.173 0.748 MTNR1B-Tango −0.324 0.242 −0.172 0.214 −0.261 −0.532 0.612 0.733 NMBR-Tango 0.141 0.074 0.455 0.134 −0.291 −0.257 0.284 0.733 NMUR1-Tango −0.418 0.116 0.186 0.318 −0.930 −1.130 −0.650 −0.087 NMUR2-Tango −0.780 0.357 −0.145 1.170 −0.445 −0.992 0.894 1.085 NPBW1-Tango −0.543 −0.848 −0.750 0.564 −0.127 −0.196 0.313 −0.199 NPBW2-Tango −0.592 −0.249 −0.498 0.640 −0.733 −0.011 −0.570 −0.429 NPFF1-Tango −0.052 0.074 −0.070 −1.074 0.033 −0.665 −0.608 −0.293 NPFF2-Tango −0.358 −0.194 0.124 0.062 −0.262 −0.480 −0.041 0.673 NPS-Tango −0.429 −0.311 −0.202 −0.426 −0.292 −0.149 −0.215 −0.087 NPY1R-Tango 0.093 0.026 −0.130 −0.031 −0.087 −0.236 −0.381 −0.326 NPY2R-Tango 0.262 −0.178 −0.055 0.155 −0.449 −0.088 −0.171 0.265 NPY4R-Tango −0.423 −0.486 −0.177 −0.017 −0.435 −0.720 0.379 0.360 NPY5R-Tango −0.047 0.012 0.208 0.065 −0.778 −0.790 0.063 0.197 NTSR1-Tango −0.298 0.029 0.085 0.401 −1.176 −0.789 0.323 0.263 NTSR2-Tango 0.064 0.413 0.265 0.396 −0.492 −0.863 0.137 0.401 OPN3-Tango 0.103 0.000 −0.152 0.153 −0.055 0.000 0.000 −0.347 OPN5-Tango −0.695 −0.262 −0.281 0.477 −0.865 −0.871 0.122 0.321 OPRD1-Tango −0.521 −0.390 −0.616 0.299 −0.636 −1.390 −0.219 0.330 OPRK1-Tango 0.514 0.532 0.356 0.437 −0.151 0.082 0.402 0.560 OPRL1-Tango 0.026 0.243 −0.091 −0.336 −0.202 −0.563 0.846 0.505 OPRM1-Tango 0.299 0.012 0.610 0.299 −0.284 −0.365 −0.313 −0.291 OXER1-Tango 0.255 0.199 0.355 0.261 −0.595 0.241 −0.324 −0.386 OXGR1-Tango −1.044 −0.437 −0.165 0.194 −0.935 −1.101 0.203 0.298 OXTR-Tango 0.074 −0.160 0.027 0.157 −0.094 −1.110 0.162 0.162 P2RY1-Tango −0.480 0.099 −0.090 0.421 −1.167 −0.780 0.019 0.432 P2RY10-Tango −0.121 0.304 0.149 −0.222 −0.619 −1.031 0.224 0.069 P2RY11-Tango −0.179 −0.248 −0.050 0.287 −0.645 −1.214 −0.260 0.019 P2RY12-Tango −0.230 −0.081 0.069 0.494 −1.236 −0.937 0.401 0.468 P2RY13-Tango −0.770 −0.433 −0.664 0.293 −0.451 −0.261 0.436 0.526 P2RY14-Tango −0.517 −0.755 −0.729 −0.059 −0.449 −0.067 0.244 −0.035 P2RY2-Tango −0.660 −0.387 −0.750 0.187 −0.777 −0.842 −0.002 −0.207 P2RY4-Tango −0.975 −0.965 −0.920 0.326 −0.631 −0.820 0.190 0.318 P2RY6-Tango −0.719 −0.881 −0.708 −0.080 −0.728 −0.648 0.333 0.625 P2RY8-Tango −0.553 −0.745 −0.471 −0.306 −0.434 −0.581 0.012 0.460 PK1-Tango −0.500 −0.635 −0.384 −0.255 0.087 −0.373 −0.128 0.118 PK2-Tango −0.128 −0.330 0.118 0.263 0.000 −0.626 0.125 0.125 PRRP-Tango −0.375 −0.444 −0.395 0.301 −0.727 −0.070 0.848 0.379 PTAFR-Tango −0.175 −0.177 −0.115 0.364 −0.685 −0.020 0.715 0.823 PTGDR-Tango −0.008 −0.080 −0.222 0.076 −0.532 −0.792 0.033 0.164 PTGER1-Tango 0.401 0.413 0.082 0.553 −0.273 −0.229 0.191 0.085 PTGER2-Tango 0.165 0.296 −0.156 0.494 −0.798 −0.703 0.822 0.792 PTGER3-Tango −0.099 0.147 0.219 0.453 −0.553 −0.367 0.680 0.748 PTGER4-Tango 0.386 0.780 0.788 0.494 0.372 0.431 0.648 0.615 PTGFR-Tango −0.054 −0.258 −0.143 0.848 −0.106 −0.200 0.523 0.310 PTGIR-Tango −0.785 −0.073 −0.550 0.288 −0.372 −1.250 0.623 0.463 PTH1R-Tango −0.274 −0.010 −0.010 0.038 −0.521 −0.731 0.177 0.570 PTH2R-Tango −0.523 −0.603 −0.561 0.087 −0.533 −0.928 0.265 0.327 QRFP-Tango −0.039 −0.147 −0.155 0.266 −0.817 −1.152 0.053 0.196 RXFP1-Tango −0.205 −0.209 −0.320 0.102 −0.310 −1.234 −0.706 −0.817 RXFP2-Tango −1.088 −0.893 −1.081 0.056 −0.782 −0.904 −0.141 −0.086 RXFP3-Tango −0.624 −0.262 −0.702 −0.093 −0.425 −1.194 −0.020 −0.139 RXFP4-Tango −0.362 −0.386 −0.666 0.350 −0.645 −0.645 0.208 0.405 S1PR1-Tango −0.070 0.020 0.240 0.589 −0.663 −0.235 0.038 0.218 S1PR2-Tango −0.655 −0.118 −0.264 0.503 −0.515 −0.185 −0.019 0.258 S1PR3-Tango 0.375 0.478 0.527 0.610 −0.684 −0.543 0.230 0.537 S1PR4-Tango −1.248 −0.733 −0.488 0.180 −0.918 −0.737 0.398 0.553 S1PR5-Tango −0.453 −0.713 −0.199 0.000 −0.585 −0.415 0.376 0.376 SCTR-Tango −0.064 −0.246 0.150 0.535 −0.100 0.109 1.212 1.243 SSTR1-Tango 0.143 0.143 0.372 0.500 −0.170 −0.082 0.596 0.918 SSTR2-Tango 0.246 0.076 0.628 0.340 −0.244 0.318 −0.661 0.000 SSTR3-Tango 0.377 0.357 0.077 0.645 0.501 0.376 0.940 1.250 SSTR4-Tango −0.021 0.114 0.255 −0.064 0.206 0.545 0.303 0.848 SSTR5-Tango 0.562 0.543 0.460 0.640 1.000 1.918 0.742 0.857 SUCNR1-Tango 2.594 2.560 2.697 −0.075 3.681 4.105 2.733 3.230 TA1-Tango −0.090 −0.265 −0.135 0.313 0.047 0.273 0.603 0.852 TAAR2-Tango 0.057 0.014 0.165 −0.160 −0.422 −0.415 0.070 0.520 TAAR5-Tango −0.241 −0.271 −0.438 −0.860 −0.390 −0.526 −0.157 −0.157 TAAR6-Tango −0.269 −0.258 −0.334 −0.484 −0.073 −0.037 −0.356 −0.316 TAAR8-Tango −0.625 0.191 0.135 −0.721 −0.225 −0.213 −0.238 −0.365 TAAR9-Tango −0.376 −0.196 −0.380 −0.715 −0.030 −0.311 −0.357 −0.426 TACR1-Tango −0.255 −0.218 −0.417 −0.412 −0.261 0.158 0.457 0.370 TACR2-Tango −0.119 −0.175 −0.206 −0.299 −0.318 −0.252 0.015 0.330 TACR3-Tango −0.086 0.047 0.119 −0.043 −0.675 −0.484 0.479 0.514 TBXA2R-Tango −0.243 −0.220 −0.419 −0.192 −0.131 −0.176 0.212 −0.100 TSHR-Tango −0.403 −0.395 −0.295 −0.167 −0.295 −0.142 −0.662 −0.662 UTS2R-Tango −0.055 0.190 −0.343 −0.116 0.260 −0.144 −0.490 −0.299 VIPR1-Tango −0.156 −0.090 0.053 −1.117 −0.331 −0.023 −0.700 −0.700 VIPR2-Tango 0.348 0.221 0.401 0.030 0.545 1.250 0.718 1.196 (Enterobac- (Enterobac- (Enterobac- (Enterobac- (Enterobac- (Enterobac- (Enterobac- PRESTO-Tango teria)UC/ teria)UC/ teria)UC/ teria)UC/ teria)UC/ teria)UC/ teria)UC/ with GMM UC-14# UC-15# UC-16# UC-17# UC-18# UC-19# UC-20# ADCYAP1R1- 0.475 −0.223 −0.137 0.864 0.380 0.245 0.210 Tango ADORA1-Tango −0.981 −0.212 −1.163 0.945 0.035 −0.363 −0.545 ADORA2A-Tango −0.457 −0.700 −0.396 0.258 0.080 −0.178 −0.248 ADORA2B-Tango −0.194 −0.877 −0.210 −0.134 −0.087 −0.462 −0.285 ADORA3-Tango −0.036 −0.379 −0.060 0.066 0.016 −0.245 −0.373 ADRA1A-Tango 0.093 −0.389 −0.118 0.664 −0.023 −0.282 −0.268 ADRA1B-Tango −0.692 −1.055 −0.200 0.295 −0.213 −0.351 −0.494 ADRA1D-Tango −0.292 −0.244 −0.318 0.731 −0.450 −0.754 −0.543 ADRA2A-Tango −0.410 −0.129 −0.490 3.260 0.375 0.226 0.236 ADRA2B-Tango −1.033 −0.788 −1.467 1.971 0.009 −0.442 −0.273 ADRA2C-Tango 0.477 0.631 −0.125 3.097 0.733 0.367 0.304 ADRB1-Tango −0.383 0.559 0.165 2.055 0.961 0.982 0.877 ADRB2-Tango −0.528 −0.047 0.054 0.463 0.663 −0.028 0.709 ADRB3-Tango 0.204 −0.812 −0.022 0.452 0.262 0.280 0.207 AGTR1-Tango 0.157 0.298 −0.125 0.334 0.370 0.499 0.313 AGTR2-Tango 0.051 0.689 0.185 0.932 0.735 0.628 0.625 APJ-Tango −0.441 −0.297 −0.275 1.096 0.655 0.644 0.175 AVPR1A-Tango −0.520 −0.940 −0.296 0.097 0.086 −0.191 −0.494 AVPR1B-Tango −0.219 −0.132 0.135 0.611 0.293 0.116 −0.351 AVPR2-Tango −0.661 0.113 −0.611 1.026 0.267 −0.108 −0.098 BB3-Tango −0.560 −0.229 −0.235 1.159 0.489 0.284 0.064 BDKBR1-Tango −0.057 −0.426 0.172 1.408 0.671 0.540 0.342 BDKBR2-Tango 0.046 0.264 0.272 0.309 0.739 0.481 0.195 C3AR1-Tango −0.187 −0.259 −0.354 0.469 0.097 0.088 0.012 C5A-Tango −0.720 −0.478 0.245 0.270 0.604 0.311 0.124 CALCRb-Tango −0.443 −0.838 −0.042 −0.031 −0.071 −0.329 −0.212 CALCRL-Tango −0.047 −0.779 −0.180 −0.072 0.021 0.037 −0.008 CASR-Tango −1.354 −1.444 −0.055 0.276 0.267 0.184 −0.010 CCKAR-Tango −0.383 −0.317 −0.074 −0.051 0.115 0.028 −0.324 CCKBR-Tango −1.082 −0.885 −0.362 1.348 0.015 0.118 −0.096 CCR10-Tango −1.091 −0.398 −0.255 0.444 0.159 0.310 0.114 CCR2-Tango −1.444 −1.194 −0.065 1.065 0.314 0.262 0.171 CCR3-Tango −0.326 1.462 −0.672 0.795 0.313 0.050 −0.244 CCR4-Tango −0.594 0.238 −0.100 0.670 0.584 0.190 0.177 CCR5-Tango −0.029 0.784 −0.095 −0.033 0.357 −0.039 −0.230 CCR6-Tango 0.209 1.039 0.011 0.994 0.352 0.079 −0.122 CCR7-Tango −0.292 0.208 −0.219 0.069 0.156 −0.174 −0.185 CCR8-Tango −0.042 0.181 0.073 1.044 0.175 −0.144 −0.230 CCRL2-Tango 0.044 −0.318 −0.267 0.317 0.158 0.033 0.592 CD97-Tango −0.660 0.091 −0.160 2.573 0.795 0.748 0.160 CHRM1-Tango 0.136 −0.040 −0.472 0.801 0.379 0.477 −0.026 CHRM2-Tango −0.859 −0.556 0.082 1.375 0.718 0.534 0.457 CHRM3-Tango −0.280 0.109 −0.062 1.679 0.552 0.469 0.314 CHRM4-Tango 0.348 −0.106 0.122 0.345 0.507 0.436 0.403 CHRM5-Tango 0.000 0.436 0.238 0.174 0.881 0.643 0.423 CMKLR1-Tango −0.304 −0.607 −0.033 0.341 0.328 0.384 0.226 CNR1-Tango −0.086 −0.189 −0.146 0.144 0.113 −0.234 −0.251 CNR2-Tango 0.055 −0.257 −0.345 −0.047 −0.047 −0.012 −0.362 CRHR1-Tango −0.160 −0.407 0.000 0.802 0.167 0.188 0.147 CRHR2-Tango 0.445 −0.047 0.118 0.491 0.207 0.129 0.140 CX3C1-Tango −0.069 −0.445 −0.102 0.521 0.514 0.302 0.082 CXCR1-Tango 0.620 0.602 0.239 0.455 0.624 0.557 0.224 CXCR2-Tango 1.019 1.652 0.490 0.448 1.035 0.887 0.648 CXCR3-Tango 0.205 0.282 −0.050 0.835 0.788 0.513 0.313 CXCR4-Tango 0.185 0.660 0.118 0.244 0.424 0.467 0.359 CXCR5-Tango −0.010 −0.559 −0.032 0.785 0.371 0.398 0.413 CXCR6-Tango 1.235 1.310 −0.010 0.946 0.859 0.588 0.693 CXCR7-Tango −0.206 0.896 −0.411 1.194 0.945 0.980 0.653 CYSLTR1-Tango 0.731 1.218 −0.284 0.621 0.492 0.487 0.148 CYSLTR2-Tango 0.570 0.150 0.311 0.890 0.775 0.738 0.473 DRD1-Tango 0.750 0.360 −0.099 0.986 0.384 0.350 0.051 DRD2-Tango 0.629 0.506 −0.073 4.161 1.146 0.789 3.080 DRD3-Tango 1.534 0.624 0.604 4.937 1.926 1.339 1.193 DRD4-Tango 0.655 1.040 0.877 5.482 2.078 0.887 1.028 DRD5-Tango 0.255 0.111 −0.367 0.143 −0.298 −0.373 −0.272 EDNRA-Tango −0.085 0.409 0.097 0.159 0.318 0.210 −0.050 EDNRB-Tango −0.051 −0.517 −0.063 0.181 0.496 0.173 0.249 ELTD1-Tango −0.788 −0.521 −0.334 0.803 0.586 0.361 0.141 F2R-Tango −0.042 −0.243 −0.200 0.156 −0.027 0.058 −0.350 F2RL1-Tango −0.039 −0.230 −0.090 0.003 0.177 0.031 −0.183 F2RL2-Tango −0.285 −0.009 −0.089 0.014 −0.035 −0.050 −0.257 F2RL3-Tango −0.135 0.000 −0.141 0.232 0.049 −0.116 −0.141 FFA1-Tango −0.423 0.051 0.152 0.642 0.718 0.542 0.513 FFA2-Tango −0.400 0.176 −0.242 0.837 0.231 0.021 −0.013 FFA3-Tango −0.023 0.384 0.059 0.678 0.254 0.555 0.471 FPR1-Tango 0.540 1.109 0.644 1.231 1.381 1.147 1.013 FPR2-Tango 0.111 0.313 0.483 2.992 0.836 0.508 0.301 FPR3-Tango −0.334 0.402 −0.270 0.574 0.266 0.145 0.144 FSHR-Tango −0.539 −0.262 −0.202 0.194 0.221 0.118 −0.234 GABBR1-Tango 0.038 −0.284 0.024 2.995 0.513 0.241 0.176 GAL1-Tango −0.646 −1.222 −0.293 1.984 0.700 0.225 0.412 GAL2-Tango 0.885 0.254 −0.072 0.670 0.102 −0.208 −0.392 GAL3-Tango −0.707 −0.491 −0.147 1.518 −0.280 0.170 −0.120 GCGR-Tango −0.521 −0.471 −0.402 0.553 0.077 −0.402 −0.402 GHRHR-Tango −0.336 −0.575 0.198 0.679 0.234 0.118 0.234 GHSR-Tango −0.247 −0.217 −0.816 0.470 0.020 −0.234 −0.294 GIPR-Tango −1.122 −0.840 −0.455 0.091 −0.455 −0.467 −0.591 GLP1R-Tango −0.592 −0.958 −0.705 −0.065 −0.504 −0.914 −1.447 GLP2R-Tango −0.029 −0.116 −0.325 0.142 −0.136 −0.164 −0.378 GNRHR-Tango 0.148 0.094 0.221 0.141 0.051 0.327 0.337 GPBA-Tango −0.532 0.855 0.000 1.069 0.328 0.118 0.298 GPER-Tango −0.191 0.146 0.125 1.039 0.412 0.252 0.176 GPR1-Tango −0.547 0.128 −0.191 0.650 0.424 0.424 0.281 GPR101-Tango −0.163 0.271 0.137 0.530 0.520 0.369 0.226 GPR110-Tango −0.201 −0.354 −0.434 0.814 −0.172 −0.418 −0.563 GPR111-Tango −0.108 −0.417 0.102 0.776 0.429 0.076 −0.070 GPR113-Tango −0.185 −0.166 −0.152 0.138 −0.206 −0.226 −0.491 GPR114-Tango −0.436 −0.634 −0.108 0.248 0.179 0.079 −0.036 GPR115-Tango −0.455 −0.173 −0.346 0.134 0.026 −0.023 −0.371 GPR116-Tango −0.671 −0.496 −0.405 1.028 0.042 −0.100 −0.377 GPR119-Tango 0.114 −0.123 0.013 0.462 0.212 0.055 −0.038 GPR12-Tango −0.380 0.310 0.096 1.154 0.357 0.133 0.050 GPR120-Tango −0.355 −0.571 0.008 1.719 0.466 0.448 0.514 GPR123-Tango 0.196 0.047 −0.067 0.825 0.123 −0.193 −0.196 GPR124-Tango −0.272 −0.025 −0.185 0.104 0.214 0.052 −0.024 GPR125-Tango −0.010 0.157 −0.295 −0.253 0.218 0.073 −0.085 GPR126-Tango −0.502 −0.565 −0.229 0.879 0.077 −0.159 −0.127 GPR132-Tango −0.762 −0.667 −0.551 0.482 −0.507 −0.371 −0.647 GPR133-Tango −0.458 −0.331 −0.499 −0.077 0.032 −0.111 −0.391 GPR135-Tango −0.289 −0.343 0.043 0.255 0.195 0.037 −0.116 GPR141-Tango −0.570 0.162 0.484 1.873 0.858 0.541 0.555 GPR142-Tango −0.225 −0.065 −0.119 0.301 0.179 0.019 −0.180 GPR143-Tango −0.560 0.044 −0.145 0.000 0.099 −0.090 −0.496 GPR144-Tango −1.718 −0.315 0.040 0.155 0.453 0.024 0.064 GPR146-Tango −0.217 0.246 −0.477 0.405 −0.062 0.000 −0.395 GPR148-Tango 0.280 0.684 0.160 0.631 0.282 0.082 0.234 GPR149-Tango 0.000 0.670 −0.155 0.932 0.262 0.489 0.193 GPR15-Tango −0.121 0.350 −0.311 0.938 −0.127 −0.564 −0.192 GPR150-Tango 0.112 0.000 −0.037 0.954 −0.216 −0.037 0.204 GPR151-Tango −0.592 0.212 0.524 0.459 0.501 0.392 0.347 GPR152-Tango −0.419 −0.739 −0.096 0.499 0.253 0.357 0.105 GPR153-Tango −0.136 0.239 −0.191 0.134 0.447 0.332 0.166 GPR156-Tango −0.343 −0.148 −0.132 1.433 0.274 0.328 0.094 GPR157-Tango −0.061 −0.050 −0.025 0.791 0.442 0.255 0.377 GPR158-Tango −0.400 −0.526 −0.126 0.526 −0.102 0.130 −0.140 GPR160-Tango 0.000 0.205 0.210 0.278 0.353 0.043 −0.066 GPR161-Tango −0.061 0.538 0.224 0.435 0.246 0.237 0.014 GPR162-Tango −0.424 −0.471 −0.142 0.255 0.004 −0.109 −0.145 GPR17-Tango 0.289 0.515 0.351 0.747 0.664 0.670 0.632 GPR171-Tango −0.165 0.058 0.096 0.754 0.277 0.560 0.455 GPR173-Tango −0.489 −0.184 0.209 0.561 0.535 0.614 0.407 GPR174-Tango 0.061 0.428 −0.095 0.674 0.788 0.625 0.564 GPR18-Tango −0.009 −0.249 −0.020 0.493 0.544 0.410 0.372 GPR182-Tango −0.263 0.211 −0.172 0.293 0.630 0.620 0.407 GPR183-Tango −0.369 −0.592 0.059 0.487 0.609 0.556 0.441 GPR19-Tango −0.330 −0.372 −0.001 0.333 0.560 0.219 −0.068 GPR20-Tango 0.170 −0.368 −0.263 0.737 0.350 0.328 0.328 GPR21-Tango −0.405 −0.648 0.372 0.469 0.017 −0.318 0.232 GPR22-Tango 0.358 0.295 0.204 0.148 0.543 0.168 −0.054 GPR25-Tango 0.191 −0.542 0.385 0.863 0.577 0.910 0.526 GPR26-Tango −1.410 −0.564 −0.440 0.947 0.142 0.472 0.198 GPR27-Tango 0.266 0.501 0.326 0.547 0.651 0.748 0.781 GPR3-Tango −0.033 0.722 0.112 0.655 0.536 0.646 0.716 GPR31-Tango −0.289 0.431 −0.326 0.791 0.115 0.186 0.247 GPR32-Tango −0.437 −0.080 −0.889 0.482 0.146 −0.201 −0.461 GPR34-Tango −0.766 0.300 −0.308 −0.203 −0.125 −0.299 −0.136 GPR35-Tango −0.688 0.480 0.301 1.155 0.704 0.485 0.490 GPR37-Tango −0.280 0.622 −0.221 1.371 0.288 0.499 0.358 GPR37L1-Tango −0.285 −0.360 −0.234 1.262 0.015 −0.145 −0.465 GPR39-Tango −0.743 −0.765 0.149 0.408 0.521 0.547 0.817 GPR4-Tango −0.176 0.775 0.060 0.328 0.516 0.697 0.388 GPR44-Tango −0.166 −0.096 −0.157 0.070 0.120 0.218 0.193 GPR45-Tango −0.106 −0.918 −0.436 0.987 −0.019 0.031 −0.131 GPR50-Tango 0.720 −0.258 −0.105 0.171 0.067 0.044 0.000 GPR52-Tango 0.379 −0.370 0.388 1.352 0.816 0.532 0.803 GPR55-Tango −0.516 −0.566 −0.159 1.362 0.135 0.385 0.177 GPR56-Tango −0.176 −0.759 −0.180 1.193 −0.363 −0.576 −0.941 GPR6-Tango −0.248 −0.696 0.283 0.376 0.392 0.319 0.318 GPR61-Tango 0.183 −0.073 0.284 0.531 0.536 0.528 0.503 GPR62-Tango −0.896 −0.896 −0.120 0.086 −0.400 −0.091 −0.174 GPR63-Tango 0.027 −0.594 −0.176 0.607 0.738 0.142 0.136 GPR64-Tango −0.174 −0.269 −0.413 0.315 0.335 0.004 0.016 GPR65-Tango 0.122 −0.191 −0.306 −0.030 −0.074 −0.103 −0.134 GPR68-Tango 0.035 −0.580 −0.711 −0.208 −0.493 −0.536 −0.717 GPR75-Tango 0.392 −0.490 −0.477 0.498 0.134 −0.412 −0.389 GPR77-Tango −1.013 −0.286 −0.656 0.684 −0.031 −0.260 −0.252 GPR78-Tango −1.025 −0.344 0.024 0.591 0.414 0.240 0.299 GPR82-Tango −0.566 0.671 −0.766 0.389 0.212 −0.313 −0.340 GPR83-Tango −0.424 −0.566 0.016 1.313 0.544 0.101 0.124 GPR84-Tango −0.616 0.269 −0.466 1.016 0.609 0.162 0.034 GPR85-Tango −0.226 −0.870 −0.074 0.681 0.253 0.264 0.226 GPR87-Tango −0.273 0.315 −0.129 0.427 0.572 0.340 0.281 GPR88-Tango −0.076 −0.754 0.015 0.540 0.432 0.264 0.184 GPR97-Tango −0.617 −0.058 −0.288 0.945 0.264 −0.010 −0.141 GPRC5A-Tango −0.886 −0.567 −0.419 0.942 0.074 0.026 0.013 GPRC5B-Tango −0.388 −0.450 −0.129 0.461 0.360 0.122 0.097 GPRC5C-Tango 0.218 0.368 −0.014 0.411 0.123 −0.041 0.131 GPRC5D-Tango −0.262 −0.869 −0.321 0.983 0.030 −0.064 −0.042 GPRC6A-Tango 0.020 −0.476 −0.131 0.072 0.038 −0.089 −0.243 GRM1-Tango 0.099 −0.423 0.077 0.358 0.024 −0.147 −0.332 GRM2-Tango −0.510 −0.720 −0.027 1.328 −0.225 −0.348 −0.170 GRM4-Tango −0.148 −0.468 −0.113 0.597 −0.111 −0.353 −0.507 GRM5-Tango −0.132 −0.451 −0.245 0.299 −0.205 −0.297 −0.448 GRM6-Tango 0.000 0.000 −0.204 0.166 0.235 0.294 0.166 GRM7-Tango −0.144 0.232 −0.517 −0.169 −0.169 −0.169 −0.336 GRM8-Tango −0.250 −0.862 −0.305 0.953 −0.188 −0.068 −0.630 GRPR-Tango −0.127 −0.219 −0.359 0.153 0.130 −0.257 −0.288 HCA1-Tango −0.327 −0.628 −0.246 0.582 0.382 0.465 0.153 HCA2-Tango −0.012 0.792 −0.019 1.527 0.595 0.658 0.518 HCA3-Tango 0.127 0.065 −0.376 0.716 0.470 0.280 0.462 HCTR1-Tango −0.778 −0.159 0.261 0.482 0.712 0.322 0.292 HCTR2-Tango −0.766 0.339 0.087 0.273 −0.281 −0.497 −0.126 HRH1-Tango 0.245 0.508 0.276 0.426 1.106 0.723 0.823 HRH2-Tango 0.382 0.045 0.281 0.544 0.665 0.751 1.018 HRH3-Tango 0.960 1.303 0.961 0.715 1.444 1.148 2.341 HRH4-Tango 0.782 1.451 0.726 0.671 1.567 1.281 2.012 HTR1A-Tango 0.345 0.298 0.058 1.479 0.498 0.184 0.352 HTR1B-Tango 0.708 0.907 0.110 0.694 0.669 0.545 0.400 HTR1D-Tango 0.142 0.434 0.122 0.620 0.453 0.263 0.359 HTR1E-Tango 0.061 0.661 0.052 0.459 0.484 0.379 0.238 HTR1F-Tango 0.172 0.343 −0.256 0.353 0.037 0.020 0.177 HTR2A-Tango −0.136 0.025 −0.354 1.172 0.271 0.155 0.049 HTR2B-Tango 0.143 0.235 −0.109 0.516 0.234 0.455 0.138 HTR2C-Tango −0.512 −0.939 −0.164 1.055 0.161 0.035 0.029 HTR4-Tango 0.057 −0.141 0.312 1.445 0.577 0.507 0.467 HTR5-Tango 0.320 0.817 −0.277 0.595 0.319 0.254 0.213 HTR6-Tango −0.133 −0.460 0.019 1.115 0.520 0.471 0.573 HTR7-Tango −0.033 0.130 −0.170 1.024 0.410 0.328 0.216 KISSPEPTIN-R- −0.596 −0.596 0.496 0.378 0.219 0.451 −0.194 Tango LHCGR-Tango −0.647 −1.177 −0.086 0.394 0.153 0.120 −0.075 LPAR1-Tango −0.458 0.189 0.182 1.022 0.736 0.903 0.708 LPAR2-Tango −0.766 −1.120 0.081 0.469 0.469 0.333 0.326 LPAR4-Tango −0.759 −0.849 −0.196 0.789 0.304 0.342 0.066 LPAR5-Tango −0.416 −0.677 0.276 1.460 0.636 0.523 0.382 LPAR6-Tango 0.543 0.338 0.130 0.321 0.234 0.197 0.290 LTB4R-Tango 0.234 −0.194 −0.103 0.528 0.242 0.284 0.000 LTB4R2B-Tango −0.115 0.197 0.029 0.079 0.342 −0.031 −0.129 MAS1-Tango 0.016 0.054 −0.337 0.566 0.412 0.170 0.155 MAS1L-Tango −0.613 −0.329 −0.053 −0.021 0.347 0.247 −0.036 MC1R-Tango −0.549 −0.713 0.153 0.455 0.257 0.127 0.167 MC2R-Tango 0.265 0.165 0.328 0.676 0.392 0.000 0.248 MC3R-Tango −0.025 0.112 0.245 0.916 0.541 −0.245 0.280 MC4R-Tango −0.295 0.140 0.141 1.538 0.679 0.566 0.653 MC5R-Tango −0.672 0.255 −0.057 0.633 0.788 0.336 0.170 MCHR1-Tango −0.383 0.093 0.047 0.573 0.556 0.226 0.182 MCHR2-Tango −0.328 −0.184 −0.191 −0.025 0.112 −0.088 −0.121 MLNR-Tango 0.075 −0.413 −0.128 0.273 0.243 0.230 0.168 MRGPRD-Tango 0.031 0.366 −0.223 0.226 0.502 0.133 0.171 MRGPRE-Tango 0.024 −0.147 0.563 0.636 0.557 0.571 0.356 MRGPRF-Tango −0.105 0.056 0.120 1.195 1.074 0.909 0.942 MRGPRG-Tango −0.133 −0.039 0.072 0.148 0.616 0.779 0.599 MRGPRX1-Tango −0.137 0.094 −0.053 0.779 0.470 0.224 0.136 MRGPRX2-Tango −0.296 0.536 0.098 1.086 0.638 0.401 0.349 MRGPRX3-Tango 0.295 −0.298 0.185 0.621 0.344 0.306 0.260 MRGPRX4-Tango −0.799 0.057 −1.082 1.117 0.149 −0.713 −0.968 MTNR1A-Tango −0.660 0.444 −0.238 1.103 0.806 0.364 0.239 MTNR1B-Tango −0.405 0.303 −0.511 2.202 0.575 0.407 0.272 NMBR-Tango 0.085 0.064 −0.150 1.157 0.851 0.288 0.355 NMUR1-Tango −0.263 −0.840 0.164 0.379 0.307 0.089 −0.321 NMUR2-Tango −0.874 0.710 −0.347 0.346 0.034 −0.028 −0.548 NPBW1-Tango 0.068 −0.199 0.081 0.964 0.855 0.018 −0.145 NPBW2-Tango −0.090 −0.082 −1.050 1.433 0.553 −0.086 −0.239 NPFF1-Tango 0.057 −1.012 0.293 1.033 0.854 0.410 0.425 NPFF2-Tango −0.372 −0.115 −0.135 0.390 0.447 −0.102 0.139 NPS-Tango −0.481 −1.175 −0.326 −0.042 −0.035 −0.461 −0.152 NPY1R-Tango −0.742 −0.966 −0.051 0.436 0.699 0.139 0.011 NPY2R-Tango −0.384 −0.645 −0.530 0.161 0.116 −0.263 −0.227 NPY4R-Tango −0.235 −0.798 0.012 −0.126 −0.163 −0.177 −0.260 NPY5R-Tango −0.356 −0.098 −0.135 1.087 0.454 0.316 0.254 NTSR1-Tango −0.143 0.468 −0.339 0.552 0.622 0.288 0.341 NTSR2-Tango −0.386 −0.520 −0.348 0.338 0.194 0.009 −0.097 OPN3-Tango −0.258 −0.455 0.044 0.555 0.286 0.131 0.051 OPN5-Tango −0.720 0.064 −0.475 1.664 −0.158 −0.496 −0.709 OPRD1-Tango −0.656 −0.453 −0.785 0.906 −0.215 −0.428 −0.489 OPRK1-Tango −0.058 −0.024 0.059 0.693 0.294 0.443 0.269 0PRL1-Tango 0.846 0.505 −0.035 0.486 0.196 −0.122 0.063 OPRM1-Tango −0.558 −0.518 −0.285 1.466 0.190 0.339 −0.195 OXER1-Tango −0.598 0.133 0.121 0.549 0.453 0.226 0.171 OXGR1-Tango −0.913 0.234 −0.609 1.142 0.231 −0.020 −0.297 OXTR-Tango −0.042 −0.692 0.125 0.650 0.563 0.393 0.295 P2RY1-Tango −0.295 0.267 −0.424 0.944 0.393 0.272 0.381 P2RY10-Tango −0.314 −0.392 −0.080 1.065 0.370 0.214 0.197 P2RY11-Tango −0.193 −0.658 0.124 0.739 0.433 0.124 0.085 P2RY12-Tango −0.068 0.860 −0.231 1.820 0.780 0.575 0.488 P2RY13-Tango −0.127 0.670 0.056 0.599 0.358 0.318 0.280 P2RY14-Tango −0.111 0.749 −0.067 0.569 0.391 0.276 0.297 P2RY2-Tango −0.417 −0.228 −0.065 0.735 0.317 0.239 0.091 P2RY4-Tango −0.341 0.225 −0.313 0.901 0.229 0.111 0.001 P2RY6-Tango −0.096 0.489 −0.045 0.905 0.211 0.144 0.162 P2RY8-Tango −0.093 −0.023 −0.191 0.665 0.168 −0.073 0.003 PK1-Tango −0.183 −0.619 0.086 0.384 0.258 0.265 0.265 PK2-Tango −0.216 −0.036 0.440 0.314 0.341 0.050 0.263 PRRP-Tango 0.138 −0.026 −0.371 0.778 0.287 0.140 −0.099 PTAFR-Tango 0.231 0.617 −0.218 0.615 0.585 0.549 0.355 PTGDR-Tango −0.098 −0.956 0.063 0.846 0.232 0.128 0.000 PTGER1-Tango −0.044 −0.722 −0.485 1.238 0.037 0.045 −0.063 PTGER2-Tango 0.272 0.150 0.362 1.314 0.938 0.676 0.284 PTGER3-Tango 0.019 0.019 0.179 1.363 0.530 0.634 0.363 PTGER4-Tango −0.156 0.123 0.348 0.902 0.780 0.906 0.860 PTGFR-Tango 0.310 0.117 −0.366 0.904 0.622 0.808 0.395 PTGIR-Tango −0.122 0.623 −0.122 −0.170 −0.957 −0.675 −1.138 PTH1R-Tango 0.335 −0.164 −0.240 0.465 0.262 0.174 0.109 PTH2R-Tango 0.313 0.617 −0.219 0.967 0.115 −0.119 −0.391 QRFP-Tango 0.111 0.214 0.098 0.026 0.143 0.187 −0.245 RXFP1-Tango −0.454 −0.758 0.098 0.287 −0.234 −0.369 −0.460 RXFP2-Tango −0.281 −0.101 −0.130 0.312 −0.025 0.136 −0.246 RXFP3-Tango −0.681 −0.974 −0.319 0.156 0.095 −0.426 −0.280 RXFP4-Tango 0.000 0.405 0.162 −0.214 0.020 −0.144 −0.280 S1PR1-Tango −0.309 0.179 0.792 1.324 0.686 0.575 0.717 S1PR2-Tango −0.877 0.211 0.296 0.919 0.396 0.267 0.388 S1PR3-Tango −0.160 0.144 0.130 0.522 0.261 −0.034 −0.011 S1PR4-Tango −0.873 0.904 −0.224 1.311 0.373 0.004 0.040 S1PR5-Tango 0.345 0.611 0.447 0.186 0.517 −0.103 −0.126 SCTR-Tango 0.722 1.861 0.273 1.414 1.078 0.678 0.874 SSTR1-Tango −0.282 0.415 0.255 0.873 1.224 0.363 0.675 SSTR2-Tango −0.133 −0.503 −0.298 −0.079 0.000 0.000 −0.474 SSTR3-Tango 0.697 1.057 0.500 0.484 0.484 −0.206 0.087 SSTR4-Tango 0.303 0.397 −0.276 0.837 0.025 −0.174 −0.047 SSTR5-Tango 0.742 0.529 0.042 0.714 0.737 0.246 0.354 SUCNR1-Tango 2.847 3.291 2.872 1.134 3.876 0.185 −0.125 TA1-Tango 0.642 0.534 −0.090 0.589 0.043 −0.300 −0.077 TAAR2-Tango 0.284 −0.404 0.049 0.042 0.008 0.104 −0.156 TAAR5-Tango −0.373 −0.661 −0.354 −0.431 −0.273 −0.237 −0.258 TAAR6-Tango −0.568 0.025 −0.541 −0.541 −0.088 −0.479 −0.071 TAAR8-Tango −0.379 −0.578 −0.039 0.087 0.373 0.528 0.129 TAAR9-Tango −0.581 −0.809 −0.030 0.353 0.495 0.450 0.179 TACR1-Tango −0.094 0.474 0.318 1.168 0.689 0.697 0.557 TACR2-Tango −0.125 −0.280 0.317 1.122 0.731 0.731 0.387 TACR3-Tango −0.618 0.278 −0.236 1.012 0.364 0.156 0.032 TBXA2R-Tango −0.302 −0.358 0.154 0.317 0.442 0.081 0.081 TSHR-Tango −0.752 −0.803 −0.562 −0.187 −0.534 −0.609 −0.886 UTS2R-Tango −0.969 −1.447 −0.246 0.051 −0.115 −0.525 −0.413 VIPR1-Tango −1.033 −0.822 0.091 1.576 0.232 0.633 0.341 VIPR2-Tango 1.600 2.101 0.177 1.014 1.105 1.118 1.447

TABLE 3 PRESTO-Tango with Gifu Medium Erysipelo- Erysipelo- Erysipelo- PRESTO-Tango Gifu trichaceae trichaceae trichaceae Ruminococcus Ruminococcus Ruminococcus Blautia with Gifu Medium Medium spp-1# spp-2# spp-3# gnavus-1# gnavus-2# torques producta GPR1-Tango 0 0.411 0.307 0.319 0.978 0.562 −0.184 0.156 GPR101-Tango 0 0.544 0.454 0.671 0.603 0.682 0.225 0.033 GPR110-Tango 0 0.292 0.262 0.203 −0.025 0.065 0.220 0.125 GPR111-Tango 0 0.745 0.373 0.252 0.786 0.252 0.286 0.341 GPR113-Tango 0 −0.127 0.171 −0.947 0.448 0.046 −0.149 −0.384 GPR114-Tango 0 −0.420 0.515 0.325 0.759 −0.186 0.165 −0.554 GPR115-Tango 0 −0.014 −0.100 −0.570 0.028 0.070 −0.108 −0.057 GPR116-Tango 0 0.250 0.263 −0.039 0.301 0.291 0.165 0.151 GPR119-Tango 0 0.911 0.189 1.029 −0.152 0.566 −0.059 0.310 GPR12-Tango 0 −0.232 0.106 0.141 1.208 0.828 −0.508 −0.124 GPR120-Tango 0 0.878 −0.122 1.112 1.463 1.000 −0.122 1.463 GPR123-Tango 0 0.590 0.590 0.126 0.403 0.340 −0.376 0.298 GPR124-Tango 0 −0.048 0.492 1.405 1.165 0.927 0.047 0.122 GPR125-Tango 0 −0.103 −0.154 0.211 −0.067 −0.097 −0.145 −0.260 GPR126-Tango 0 0.333 0.588 1.096 0.688 0.620 −0.412 0.008 GPR132-Tango 0 −0.168 −0.139 −0.788 −0.666 −0.557 0.444 −0.258 GPR133-Tango 0 0.064 0.129 −0.412 −0.018 0.123 −0.047 −0.067 GPR135-Tango 0 0.053 0.218 −0.383 −0.231 −0.156 −0.674 −0.846 GPR141-Tango 0 0.200 −0.104 −0.449 1.532 0.971 −0.449 −0.449 GPR142-Tango 0 0.293 0.509 −0.209 0.960 0.415 0.920 0.472 GPR143-Tango 0 0.235 0.312 −1.274 0.037 −0.109 0.297 0.129 GPR144-Tango 0 −0.032 0.323 2.738 −0.560 −0.272 0.196 −0.112 GPR146-Tango 0 −0.234 0.000 0.000 1.350 0.485 −0.234 0.000 GPR148-Tango 0 0.783 0.274 −0.104 0.381 0.381 0.528 −0.104 GPR149-Tango 0 1.034 1.126 2.198 1.034 0.899 0.336 0.695 GPR15-Tango 0 −0.252 0.392 −0.381 −0.556 −0.506 −1.012 −1.133 GPR150-Tango 0 −0.241 1.000 0.387 −0.241 1.107 0.107 0.823 GPR151-Tango 0 −0.076 −0.144 0.410 0.311 −0.426 −0.426 −0.191 GPR152-Tango 0 0.116 −0.329 −0.187 −0.139 −0.057 0.317 0.466 GPR153-Tango 0 0.011 0.042 −0.891 0.405 0.286 0.213 −0.024 GPR156-Tango 0 0.890 1.129 1.355 1.000 0.809 0.224 0.737 GPR157-Tango 0 −0.039 0.384 −0.640 1.147 0.508 0.082 0.541 GPR158-Tango 0 −0.102 0.168 0.010 −0.295 0.096 −0.040 0.068 GPR160-Tango 0 0.351 0.261 −0.041 0.116 0.260 −0.203 −0.071 GPR161-Tango 0 0.022 −0.129 0.100 0.344 0.273 −0.195 −0.207 GPR162-Tango 0 −0.140 0.193 −0.140 0.819 0.059 −0.685 0.059 GPR17-Tango 0 0.111 0.127 −0.352 0.404 0.342 0.008 −0.006 GPR171-Tango 0 0.564 0.655 −0.520 −0.097 0.044 0.090 0.395 GPR173-Tango 0 1.246 0.381 −0.104 1.407 0.783 0.528 0.381 GPR174-Tango 0 0.387 0.300 −0.136 0.072 −0.331 0.429 0.300 GPR18-Tango 0 0.273 0.808 −0.450 0.637 0.553 0.273 0.839 GPR182-Tango 0 0.508 0.447 0.613 0.690 0.399 0.020 −0.290 GPR183-Tango 0 0.340 0.114 −0.297 0.095 −0.080 −0.231 −0.161 GPR19-Tango 0 0.440 0.475 0.137 −0.006 0.010 −0.092 0.302 GPR20-Tango 0 −0.801 −0.170 −1.231 −0.329 −0.801 −0.545 −0.082 GPR21-Tango 0 1.000 0.888 0.888 0.632 −0.515 −1.737 1.263 GPR22-Tango 0 0.422 0.414 0.458 0.313 0.590 0.070 0.060 GPR25-Tango 0 1.112 1.697 1.112 1.217 −0.122 0.491 1.697 GPR26-Tango 0 0.868 0.846 0.519 0.791 0.742 −0.022 0.611 GPR27-Tango 0 −1.369 −1.369 −2.369 −0.147 −0.784 −0.147 −0.495 GPR3-Tango 0 0.361 0.368 0.521 1.234 0.895 −0.131 −0.017 GPR31-Tango 0 0.419 0.386 0.543 0.978 0.747 −0.129 −0.370 GPR32-Tango 0 1.007 0.732 −0.486 2.107 1.519 1.052 1.308 GPR34-Tango 0 0.255 0.476 0.787 1.255 1.393 0.850 0.476 GPR35-Tango 0 0.445 0.413 −0.296 1.177 0.752 0.404 0.396 GPR37-Tango 0 0.468 0.491 0.068 1.221 1.132 0.243 0.503 GPR37L1-Tango 0 0.474 0.454 −0.287 0.290 0.575 0.403 0.593 GPR39-Tango 0 0.087 0.629 0.267 −0.328 0.087 0.150 0.022 GPR4-Tango 0 0.160 0.138 0.352 0.586 0.379 −0.155 0.190 GPR44-Tango 0 0.609 0.544 0.205 1.577 0.702 0.790 0.609 GPR45-Tango 0 0.190 0.455 −1.400 0.982 0.236 −0.407 0.331 GPR50-Tango 0 0.553 0.620 0.125 0.813 0.093 0.291 0.345 GPR52-Tango 0 0.885 0.796 0.338 0.830 0.830 −0.148 0.188 GPR55-Tango 0 −0.049 0.130 −0.227 0.408 0.021 −0.117 0.052 GPR56-Tango 0 0.606 0.612 0.825 0.772 0.433 −0.209 0.411 GPR6-Tango 0 0.825 0.989 1.181 1.443 1.158 −0.092 −0.037 GPR61-Tango 0 −0.557 −0.187 −0.164 −0.426 −0.383 −0.784 −0.459 GPR62-Tango 0 0.485 −0.152 0.485 0.485 0.485 −0.152 0.138 GPR63-Tango 0 0.170 0.187 −1.100 0.254 0.280 0.008 −0.058 GPR64-Tango 0 −0.151 −0.134 0.407 0.057 −0.003 −0.281 −0.328 GPR65-Tango 0 −0.065 −0.080 −0.040 −0.198 −0.210 −0.009 −0.157 GPR68-Tango 0 0.026 0.068 −0.544 −0.519 −0.638 0.166 −0.150 GPR75- Tango 0 0.107 0.132 −0.281 0.294 0.076 −0.872 0.105 GPR77-Tango 0 0.595 0.380 0.681 0.738 0.943 −0.587 0.322 GPR78-Tango 0 −0.187 0.282 0.245 0.442 0.357 −0.257 0.018 GPR82-Tango 0 0.310 0.068 0.638 1.036 0.842 −0.360 −0.284 GPR83-Tango 0 0.011 0.079 −1.500 −0.006 0.095 0.320 0.253 GPR84-Tango 0 0.321 0.140 0.030 0.561 0.543 0.042 0.099 GPR85-Tango 0 0.459 0.551 −0.163 0.677 0.722 0.311 0.336 GPR87-Tango 0 0.049 0.131 −0.482 0.805 0.634 0.109 0.223 GPR88-Tango 0 −0.163 0.252 0.218 0.636 0.339 −0.915 −0.391 GPR97-Tango 0 1.023 1.149 1.521 1.564 1.127 −0.016 0.191 GPRC5A-Tango 0 −0.206 −0.144 −0.047 0.093 −0.124 0.011 0.075 GPRC5B-Tango 0 0.045 0.203 −0.143 0.311 0.328 −0.016 −0.348 GPRC5C-Tango 0 0.265 0.047 −1.061 −0.158 0.054 −0.153 0.023 GPRC5D-Tango 0 0.413 0.472 −0.665 0.369 0.277 0.071 0.273 GPRC6A-Tango 0 −0.048 −0.064 −2.030 −0.245 −0.118 0.044 −0.233 GRM7-Tango 0 −0.202 −0.231 −0.957 −0.213 −0.261 −0.528 −0.240 GRM8-Tango 0 −0.113 −0.321 −0.333 −0.122 −0.109 −0.129 −0.125 GRPR-Tango 0 0.013 −0.308 −1.919 −0.200 0.079 −0.394 −0.269 PRESTO-Tango Faecalibacterium Oscillospira Oscillospira Oscillospira Lactobacillus Lactobacillus Lactobacillus Lactobacillus with Gifu Medium prausnitzii spp-1# spp-2# spp-3# reuteri-1# reuteri-2# reuteri-3# UC GPR1-Tango −0.284 −0.022 −0.559 −1.170 0.123 0.361 0.107 −0.241 GPR101-Tango −0.102 −0.045 −0.045 −0.349 0.159 0.074 0.074 0.268 GPR110-Tango −0.272 −0.279 −0.563 −0.587 0.224 −0.226 0.112 −0.011 GPR111-Tango 0.485 1.297 0.436 0.543 0.695 0.809 0.216 0.308 GPR113-Tango −0.172 −0.007 −0.242 −0.686 0.487 −0.149 −0.172 −0.258 GPR114-Tango 0.165 0.580 −0.016 0.247 −0.298 0.325 −0.700 0.077 GPR115-Tango 0.017 −0.290 −0.413 −0.524 −0.056 −0.123 −0.097 −0.120 GPR116-Tango −0.072 0.259 −0.317 −0.515 0.265 0.165 0.134 0.070 GPR119-Tango 0.263 1.287 0.660 0.864 0.986 0.623 0.444 0.506 GPR12-Tango −0.083 −0.215 −0.099 −0.330 −0.367 −0.417 −0.156 −0.628 GPR120-Tango 0.112 1.217 1.282 −0.402 1.000 0.491 0.314 −0.122 GPR123-Tango −0.294 −0.211 −1.015 −0.633 0.015 −0.051 −0.294 −0.316 GPR124-Tango 0.165 0.547 0.206 0.580 0.000 0.750 0.122 0.672 GPR125-Tango −0.029 −0.344 −0.335 −0.628 −0.203 −0.163 −0.111 −0.388 GPR126-Tango 0.259 0.081 −0.256 −0.751 −0.349 0.126 0.024 0.725 GPR132-Tango −0.168 −0.490 −0.960 −1.133 0.050 0.170 0.204 −0.192 GPR133-Tango −0.045 −0.347 −0.235 −0.594 −0.050 0.024 −0.008 −0.097 GPR135-Tango −1.011 −1.733 −1.885 −1.231 −1.181 −1.490 −0.820 −0.585 GPR141-Tango −0.104 −0.636 −0.449 −0.571 −0.825 −0.354 −0.199 −0.508 GPR142-Tango 0.472 0.415 0.160 0.960 −0.472 0.228 0.835 0.415 GPR143-Tango −0.146 −0.004 −0.534 −0.664 0.615 0.250 0.140 −0.136 GPR144-Tango −0.006 0.229 −0.617 0.173 −0.154 0.373 −0.072 −0.367 GPR146-Tango 0.485 −0.515 −0.234 −0.515 1.170 −0.234 0.202 0.379 GPR148-Tango −0.339 0.528 0.783 −0.104 0.097 0.274 0.528 0.783 GPR149-Tango −0.157 1.213 0.336 0.771 0.791 0.637 0.203 0.899 GPR15-Tango −1.012 −0.878 −1.878 −0.816 −0.381 −0.608 −0.381 −0.293 GPR150-Tango 1.107 −0.241 0.823 0.823 0.387 −1.115 0.387 0.621 GPR151-Tango −0.513 −0.144 −0.191 −0.265 0.206 −0.672 −0.144 0.531 GPR152-Tango −0.259 0.735 0.356 0.174 0.344 0.247 0.293 0.123 GPR153-Tango 0.096 −0.110 −0.328 −0.501 −0.070 0.379 0.122 −0.048 GPR156-Tango 0.525 0.779 0.674 −0.048 0.694 0.768 0.539 0.394 GPR157-Tango −0.459 0.811 −0.087 0.167 −0.337 0.126 −0.138 0.082 GPR158-Tango −0.635 0.294 −0.517 −1.040 −0.259 0.068 0.294 −0.071 GPR160-Tango 0.025 −0.411 −1.085 −1.008 0.210 0.264 0.186 −0.255 GPR161-Tango −0.100 −0.839 −0.959 −1.130 −0.280 −0.093 −0.067 −0.338 GPR162-Tango −0.685 −0.807 −1.807 −1.573 −0.310 −0.469 −0.573 0.012 GPR17-Tango −0.078 −0.028 0.061 −0.456 0.118 0.160 0.038 −0.054 GPR171-Tango −0.077 0.631 −0.275 −0.027 0.740 0.600 0.141 −0.253 GPR173-Tango −0.339 1.066 0.097 −0.339 −0.339 −0.104 0.097 0.528 GPR174-Tango 0.269 0.469 −0.613 0.018 0.174 0.222 −0.453 −0.331 GPR18-Tango 0.171 0.678 0.126 0.292 0.470 0.139 0.207 −0.280 GPR182-Tango −0.316 −0.187 −0.191 −0.667 −0.063 −0.311 −0.316 −0.116 GPR183-Tango −0.911 −0.446 −0.937 −0.184 −0.231 0.076 0.017 −0.272 GPR19-Tango 0.234 −0.790 −1.294 −1.186 0.391 0.274 0.310 −0.065 GPR20-Tango 0.078 0.267 −0.801 −0.948 −1.433 −0.433 −0.801 −0.329 GPR21-Tango 0.766 1.511 1.000 1.350 1.433 0.379 0.632 1.104 GPR22-Tango 0.142 −0.201 −0.424 −0.537 0.060 0.255 −0.247 0.152 GPR25-Tango −0.122 0.745 0.112 0.878 0.598 1.000 1.463 0.314 GPR26-Tango 0.235 0.640 0.550 0.198 0.658 0.566 0.595 0.725 GPR27-Tango −0.147 −0.495 0.133 0.368 −0.495 0.746 0.368 −1.369 GPR3-Tango 0.120 −0.340 −0.264 −0.774 −0.170 −0.295 −0.136 −0.136 GPR31-Tango 0.258 −0.614 −1.549 −0.370 −0.549 −0.730 −1.052 0.036 GPR32-Tango 0.708 0.862 −0.312 0.189 1.114 1.026 0.997 0.720 GPR34-Tango 0.365 1.255 0.286 0.962 0.728 0.943 0.829 0.683 GPR35-Tango 0.330 0.132 −0.271 −0.285 0.663 0.787 0.436 0.261 GPR37-Tango 0.415 0.184 0.245 0.006 0.187 0.414 0.296 0.173 GPR37L1-Tango 0.432 0.319 −0.326 −0.222 0.626 0.607 0.552 0.277 GPR39-Tango −0.046 −0.093 −0.093 0.585 −0.093 −0.167 −0.046 −0.328 GPR4-Tango 0.213 −0.667 −0.617 −0.845 −0.312 −0.038 0.021 −0.070 GPR44-Tango 0.790 1.421 0.205 −0.561 −0.182 0.609 −0.075 0.609 GPR45-Tango 0.117 0.023 −0.835 −0.867 0.381 0.117 0.040 −0.470 GPR50-Tango 0.263 0.966 −0.200 −0.487 0.774 0.635 0.291 0.319 GPR52-Tango −0.082 0.040 −0.744 0.134 0.338 0.097 −0.263 0.624 GPR55-Tango −0.081 −0.216 −0.322 −0.766 −0.089 0.458 0.094 −0.216 GPR56-Tango 0.149 −0.220 −0.508 −0.592 0.375 0.230 0.259 0.661 GPR6-Tango 0.287 0.026 −0.272 −0.473 −0.072 0.114 0.054 −0.224 GPR61-Tango −0.862 −0.754 −1.299 −0.992 −0.215 −0.643 −0.773 −0.537 GPR62-Tango 1.202 0.766 1.202 1.379 0.766 0.485 −0.737 −0.152 GPR63-Tango 0.104 −0.127 −0.001 −0.168 0.113 0.135 0.158 −0.229 GPR64-Tango −0.182 −0.095 −0.261 −0.419 0.083 −0.085 0.013 −0.343 GPR65-Tango −0.076 −0.413 −0.440 −0.608 −0.204 −0.145 −0.252 −0.152 GPR68-Tango −0.406 −0.599 −1.205 −0.838 0.073 −0.083 −0.044 −0.321 GPR75- Tango −0.399 −0.636 −1.242 −1.074 0.057 −0.016 −0.151 −0.293 GPR77-Tango 0.022 −0.736 −1.021 −1.030 0.025 0.127 0.008 −0.184 GPR78-Tango −0.150 −0.678 −0.475 −0.939 −0.491 −0.228 −0.609 0.103 GPR82-Tango 0.011 −0.478 −0.901 −0.814 −0.535 −0.129 −0.348 −0.334 GPR83-Tango −0.171 0.009 −0.720 −0.651 0.301 0.187 0.262 −0.190 GPR84-Tango 0.067 −0.313 −0.470 −0.568 0.152 0.214 0.067 0.059 GPR85-Tango 0.160 0.251 −0.363 −0.564 0.224 0.405 0.311 0.023 GPR87-Tango −0.010 −0.104 −0.165 −0.234 0.007 0.186 0.126 −0.106 GPR88-Tango −0.391 −0.726 −0.726 −1.539 −0.391 −1.322 −0.163 −0.539 GPR97-Tango −0.064 0.407 0.031 −0.569 0.149 0.442 0.532 0.499 GPRC5A-Tango −0.583 −0.483 −0.861 −1.118 −0.202 −0.121 −0.090 −0.159 GPRC5B-Tango −0.097 −0.741 −1.073 −1.051 0.110 0.040 0.176 −0.354 GPRC5C-Tango −0.047 −0.044 −0.240 −0.318 −0.082 −0.053 −0.030 −0.286 GPRC5D-Tango 0.174 −0.075 −0.361 −0.533 0.413 0.453 0.267 −0.422 GPRC6A-Tango −0.126 −0.493 −0.673 −0.851 −0.118 −0.057 −0.071 −0.315 GRM7-Tango −0.178 −0.613 −0.786 −0.790 −0.059 −0.140 −0.242 −0.528 GRM8-Tango −0.386 −0.177 −0.671 −0.914 0.046 0.156 −0.162 −0.359 GRPR-Tango 0.256 0.234 −0.736 0.241 0.557 0.761 0.487 −0.065 PRESTO-Tango (Clostridiales) Pediococcus Pediococcus Pediococcus Streptococcus Streptococcus Streptococcus Streptococcus with Gifu Medium UC/UC UC-1# UC-2# UC-3# luteciae spp-1# spp-2# spp-3# GPR1-Tango −0.446 −0.439 −0.487 −0.439 0.297 0.588 −0.125 0.552 GPR101-Tango 0.039 −0.004 0.499 0.130 0.234 0.725 0.422 1.613 GPR110-Tango 0.167 0.112 −0.100 0.194 0.499 0.775 0.020 1.932 GPR111-Tango 1.753 −0.160 0.466 0.626 −0.357 0.543 0.669 −0.357 GPR113-Tango 0.046 −0.195 0.104 −0.090 −0.402 0.085 −0.384 −1.157 GPR114-Tango 0.165 −0.807 −0.016 −0.298 0.077 0.515 0.077 −0.807 GPR115-Tango −0.196 −0.241 −0.154 −0.129 −0.125 0.121 −0.024 −0.758 GPR116-Tango 0.082 0.009 0.542 0.185 0.157 0.695 0.295 −0.230 GPR119-Tango 0.566 −0.434 −0.837 0.660 1.111 0.189 1.322 0.566 GPR12-Tango −0.312 −0.986 0.429 −0.039 −0.348 −0.571 −0.886 0.798 GPR120-Tango −0.402 0.745 0.598 0.112 −0.402 1.112 1.545 −0.122 GPR123-Tango 0.214 −0.606 −0.606 −0.033 0.654 0.706 −0.746 1.975 GPR124-Tango 0.077 0.458 0.165 0.000 −0.241 1.697 0.312 3.000 GPR125-Tango −0.246 −0.158 −0.198 −0.232 −0.035 0.237 0.062 0.014 GPR126-Tango −0.256 0.208 −0.564 0.112 0.346 1.978 −0.103 2.965 GPR132-Tango −0.533 −0.027 −0.570 0.035 0.066 −0.203 −0.312 −0.513 GPR133-Tango −0.104 −0.043 0.059 0.120 −0.068 −0.023 0.137 −0.486 GPR135-Tango −0.885 −0.846 −0.674 −0.206 −0.300 −0.411 −0.585 −0.283 GPR141-Tango −0.249 −1.166 0.162 −0.249 −0.449 −0.903 −0.354 2.270 GPR142-Tango −0.368 0.293 0.293 0.509 0.760 1.325 0.665 1.954 GPR143-Tango 0.163 −0.023 −0.508 0.325 0.391 −0.102 0.067 −1.379 GPR144-Tango −0.006 −0.367 −0.006 −0.367 −0.112 −0.006 0.196 1.445 GPR146-Tango 0.485 −0.515 −0.862 0.632 0.000 1.000 −1.152 −0.234 GPR148-Tango 0.896 −1.841 −0.104 1.246 0.097 0.097 −0.619 −1.256 GPR149-Tango 0.280 −0.305 0.737 0.652 0.849 1.198 1.017 1.604 GPR15-Tango −0.943 −1.878 −1.012 −0.878 −0.171 −0.756 −1.540 1.409 GPR150-Tango 1.000 −0.241 0.107 1.107 0.387 1.107 0.107 1.387 GPR151-Tango 0.052 −0.191 0.111 0.111 −0.265 0.797 −0.144 1.833 GPR152-Tango 0.435 0.116 −0.148 0.472 0.028 −0.021 0.192 −0.078 GPR153-Tango 0.084 −0.177 0.459 0.035 0.127 −0.063 −0.043 −0.925 GPR156-Tango 0.565 −0.158 0.490 0.690 0.586 1.906 0.752 2.074 GPR157-Tango 0.682 −1.055 0.682 −0.280 −0.189 0.541 −0.337 −0.243 GPR158-Tango 0.545 −0.476 −0.370 0.039 0.245 1.314 −0.190 2.020 GPR160-Tango 0.020 −0.207 −0.461 −0.092 0.086 0.287 −0.144 0.832 GPR161-Tango −0.847 −0.188 −0.242 −0.461 −0.128 0.764 −0.479 1.147 GPR162-Tango −0.062 −0.573 −0.685 −0.469 0.128 −0.807 −0.685 0.128 GPR17-Tango −0.011 −0.002 0.099 −0.032 0.194 0.269 0.033 −0.563 GPR171-Tango 0.677 −0.849 −0.190 0.807 0.564 0.168 0.027 −0.420 GPR173-Tango 0.661 −0.339 0.274 0.783 0.097 0.661 −0.104 −0.619 GPR174-Tango 0.645 −0.076 0.072 −0.136 0.573 0.429 0.072 −0.331 GPR18-Tango 0.894 −0.874 −0.119 0.628 0.669 0.678 0.171 −0.874 GPR182-Tango 0.128 −0.149 0.966 −0.218 0.149 0.263 0.137 0.963 GPR183-Tango 0.263 −0.873 0.163 −0.348 −0.102 −0.297 −1.017 0.631 GPR19-Tango −0.063 0.149 −0.484 0.102 0.289 0.335 −0.090 1.119 GPR20-Tango 0.199 −1.231 0.000 0.333 0.000 −0.329 0.078 −0.948 GPR21-Tango 1.982 0.485 −0.515 0.632 1.433 −0.234 0.379 1.000 GPR22-Tango 0.180 −0.037 −0.247 0.080 0.770 1.031 −0.226 1.885 GPR25-Tango 0.598 0.598 0.491 1.745 1.376 −0.122 1.000 1.000 GPR26-Tango 0.734 0.150 0.530 0.884 0.630 0.782 0.323 0.150 GPR27-Tango −0.784 −1.369 −0.147 −0.495 0.133 0.133 −0.147 0.746 GPR3-Tango −0.222 −0.656 0.818 −0.080 −0.247 −0.097 −0.109 1.086 GPR31-Tango 0.158 −1.145 0.294 −0.333 0.036 −0.427 −0.487 2.699 GPR32-Tango 0.732 0.273 0.638 1.657 0.828 0.074 0.684 −0.806 GPR34-Tango 0.545 −0.279 0.620 0.765 −0.842 0.808 0.316 0.335 GPR35-Tango 0.127 0.037 0.088 0.706 0.341 0.139 0.014 −0.564 GPR37-Tango 0.407 0.221 0.643 0.455 0.167 0.471 0.456 −0.062 GPR37L1-Tango 0.316 0.261 0.391 0.547 0.555 0.387 0.590 −0.431 GPR39-Tango 0.304 0.022 −0.245 0.022 0.409 0.555 0.022 1.229 GPR4-Tango −0.110 −0.205 −0.030 0.371 0.259 0.405 0.239 1.191 GPR44-Tango 0.872 −0.561 0.072 0.609 1.672 0.790 −0.561 2.925 GPR45-Tango −0.282 −0.614 −0.147 0.190 0.190 −0.282 0.317 −2.199 GPR50-Tango 0.505 −0.585 −0.487 0.813 0.851 −0.064 0.489 0.319 GPR52-Tango −0.337 −0.263 −0.082 −0.148 0.290 −0.263 −0.848 1.874 GPR55-Tango 0.181 −0.044 −0.202 0.253 0.352 0.495 0.145 0.114 GPR56-Tango 0.205 −0.016 −0.123 0.256 0.291 1.235 0.418 1.732 GPR6-Tango 0.472 −0.211 1.190 −0.026 0.696 0.683 0.553 1.371 GPR61-Tango −0.199 −0.932 −0.613 −0.754 −0.493 −0.172 −0.819 0.733 GPR62-Tango 0.138 0.138 0.485 −1.737 0.485 1.379 1.000 1.379 GPR63-Tango −0.112 −0.342 0.385 −0.045 −0.168 0.323 0.262 −1.169 GPR64-Tango −0.229 −0.274 −0.125 −0.167 −0.316 0.113 −0.035 −0.215 GPR65-Tango −0.357 −0.095 −0.327 −0.092 0.019 0.211 −0.081 0.448 GPR68-Tango −0.195 −0.206 −0.625 0.205 0.185 −0.144 −0.173 −0.715 GPR75- Tango −0.656 −1.039 −0.900 −0.171 0.102 0.088 −0.401 0.804 GPR77-Tango −0.354 −0.627 −0.044 0.103 −0.178 0.163 −0.469 1.288 GPR78-Tango −0.064 −0.414 −0.537 −0.311 −0.281 −0.414 −0.475 1.312 GPR82-Tango −0.185 −0.549 1.050 −0.159 −0.268 −0.318 −0.224 1.545 GPR83-Tango 0.079 −0.157 −0.202 0.239 0.028 −0.149 −0.176 −1.751 GPR84-Tango 0.187 0.071 0.100 0.074 0.220 0.452 0.332 0.000 GPR85-Tango −0.253 −0.418 0.323 0.731 0.393 0.843 0.459 −0.008 GPR87-Tango 0.084 −0.065 0.169 0.158 0.078 0.261 −0.011 −0.636 GPR88-Tango −0.794 −2.225 −0.339 −0.794 −0.240 −0.794 −0.726 0.799 GPR97-Tango −0.257 −0.114 0.231 0.595 0.407 1.303 0.532 2.905 GPRC5A-Tango −0.452 −0.142 −0.500 −0.235 0.078 0.759 −0.030 1.279 GPRC5B-Tango −0.278 −0.525 −0.146 −0.642 0.176 0.620 −0.217 1.382 GPRC5C-Tango 0.053 −0.520 −0.267 0.160 0.099 0.067 −0.133 −1.202 GPRC5D-Tango −0.048 −0.215 0.064 0.388 0.424 0.363 0.158 −0.543 GPRC6A-Tango −0.400 −0.164 −0.291 −0.101 −0.037 −0.333 0.019 −1.629 GRM7-Tango −0.438 −0.432 −0.479 −0.202 −0.288 −0.194 −0.372 −1.321 GRM8-Tango −0.399 −0.194 −0.363 0.037 0.064 0.388 −0.180 −0.371 GRPR-Tango 0.256 −0.544 −0.993 0.445 −0.200 0.062 −0.230 −2.356 Acidamino- Acidamino- Acidamino- Acidamino- PRESTO-Tango coccus coccus coccus coccus Megasphaera Megasphaera Peptoniphilus Peptoniphilus with Gifu Medium spp-1# spp-2# spp-3# spp-4# spp-1# spp-2# spp-1# spp-2# GPR1-Tango −0.301 0.091 0.152 −0.027 −0.739 −0.660 0.148 −0.145 GPR101-Tango 0.045 0.261 0.274 0.189 0.164 0.093 0.242 0.164 GPR110-Tango 0.009 0.035 0.267 0.229 −0.434 0.271 0.009 0.224 GPR111-Tango 0.770 1.420 1.357 0.937 0.643 1.430 0.958 1.181 GPR113-Tango 0.066 0.153 0.516 0.303 0.558 0.346 0.607 0.189 GPR114-Tango −0.016 0.247 0.247 0.077 0.077 0.952 0.077 −0.016 GPR115-Tango −0.203 −0.180 −0.146 −0.145 −0.323 −0.168 −0.313 −0.151 GPR116-Tango 0.338 0.234 0.264 0.345 −0.131 0.234 0.317 0.282 GPR119-Tango 0.766 1.356 0.941 1.251 0.029 1.411 1.176 0.766 GPR12-Tango −1.063 −0.436 0.065 −0.312 −0.014 −0.199 −0.199 −0.109 GPR120-Tango −0.122 −1.040 0.491 −0.122 1.282 1.217 0.598 0.314 GPR123-Tango −0.353 0.110 0.126 0.298 −0.858 0.786 −0.316 0.032 GPR124-Tango 0.580 −0.601 0.547 0.515 0.832 0.721 1.016 1.016 GPR125-Tango −0.397 −0.124 −0.184 −0.213 −0.216 −0.103 −0.265 −0.350 GPR126-Tango −0.103 0.056 0.272 0.126 −0.069 0.495 0.096 0.371 GPR132-Tango −0.592 −0.449 −0.594 −0.650 −0.953 −0.468 −0.635 −0.601 GPR133-Tango 0.100 −0.208 −0.073 −0.086 −0.542 −0.366 −0.132 −0.234 GPR135-Tango −1.055 −0.383 −0.283 −1.011 −0.640 −0.710 −0.640 −0.846 GPR141-Tango −0.986 −0.150 −0.704 −0.073 −0.903 −0.508 −0.508 −0.104 GPR142-Tango −0.209 0.791 0.713 0.835 0.665 0.563 0.835 1.100 GPR143-Tango −0.219 0.052 −0.036 0.055 −0.620 0.008 −0.048 −0.129 GPR144-Tango 0.031 0.229 0.196 0.068 0.104 0.292 0.031 0.402 GPR146-Tango 0.379 −0.234 0.632 0.766 0.485 1.000 0.632 0.202 GPR148-Tango 0.097 1.159 1.481 0.097 1.246 0.661 −0.619 0.097 GPR149-Tango 0.252 1.331 1.252 1.266 2.306 1.784 1.167 1.167 GPR15-Tango −0.816 −1.211 −1.012 −0.699 −0.556 −1.059 −0.252 −0.381 GPR150-Tango 0.107 −0.241 −0.241 0.621 1.387 0.107 −0.531 0.387 GPR151-Tango 0.722 0.111 0.410 1.082 0.696 0.833 0.111 0.206 GPR152-Tango 0.389 0.404 0.389 0.617 −0.611 0.258 0.323 0.415 GPR153-Tango −0.076 0.093 −0.133 −0.170 −0.084 −0.104 −0.215 −0.166 GPR156-Tango 0.079 0.272 0.698 0.662 0.596 0.677 0.748 0.775 GPR157-Tango 0.653 1.147 0.953 0.977 1.401 0.888 0.633 0.541 GPR158-Tango −0.010 0.787 0.294 0.245 0.010 0.294 0.096 0.194 GPR160-Tango −0.559 −0.425 −0.215 −0.163 −0.418 −0.314 −0.269 −0.205 GPR161-Tango −0.588 −0.489 −0.422 −0.563 −0.357 −0.521 −0.441 −0.405 GPR162-Tango −0.807 0.059 −0.573 −0.062 0.059 −0.310 −0.807 −0.310 GPR17-Tango −0.208 −0.024 −0.048 −0.036 0.271 −0.102 0.005 0.048 GPR171-Tango −0.077 0.271 0.480 0.518 0.215 0.769 0.480 0.729 GPR173-Tango 0.896 1.066 0.097 0.783 0.661 0.274 0.783 0.274 GPR174-Tango 0.174 0.834 0.802 0.943 0.300 0.770 0.737 0.609 GPR18-Tango 0.014 0.808 1.250 1.120 1.020 0.824 0.694 0.655 GPR182-Tango 0.083 0.156 0.194 0.146 0.218 −0.142 0.123 0.037 GPR183-Tango −0.374 0.127 0.217 0.372 −0.080 −0.102 −0.161 −0.117 GPR19-Tango −0.366 −0.144 0.129 −0.045 −0.619 −0.061 −0.205 −0.123 GPR20-Tango 0.267 −0.082 0.395 0.918 −0.667 0.152 0.199 0.333 GPR21-Tango 1.104 1.350 0.379 1.104 1.170 1.982 1.511 1.945 GPR22-Tango 0.214 −0.177 0.296 0.385 0.536 0.430 0.107 0.369 GPR25-Tango 0.491 1.000 1.376 0.745 0.878 1.745 1.000 1.376 GPR26-Tango 0.904 0.904 1.325 1.296 0.734 1.262 1.117 1.358 GPR27-Tango 0.133 0.133 −1.369 −0.147 0.000 −0.147 −0.784 0.133 GPR3-Tango −0.295 −0.034 0.013 −0.113 0.507 −0.222 0.180 0.138 GPR31-Tango −1.427 −0.177 −0.804 −0.487 −0.082 −0.370 −0.007 0.158 GPR32-Tango 0.055 0.752 1.123 0.922 0.472 0.625 1.223 1.090 GPR34-Tango 0.659 1.096 0.904 0.981 0.943 0.808 0.924 1.067 GPR35-Tango −0.070 0.355 0.603 0.463 0.355 0.501 0.287 0.253 GPR37-Tango 0.087 0.369 0.323 0.272 0.421 0.200 0.219 0.355 GPR37L1-Tango 0.146 0.357 0.518 0.451 0.144 0.573 0.233 0.290 GPR39-Tango −0.678 0.672 −0.167 0.820 −0.415 0.150 −0.678 0.209 GPR4-Tango −0.165 −0.215 0.265 −0.061 −0.162 −0.182 −0.170 0.083 GPR44-Tango 0.439 0.544 0.205 1.140 0.609 −0.795 0.439 0.072 GPR45-Tango −0.166 0.538 0.149 1.112 −0.431 −0.325 0.221 0.265 GPR50-Tango 0.620 0.678 1.181 1.150 0.754 1.000 0.720 0.954 GPR52-Tango −0.497 0.585 0.338 −0.082 0.188 0.134 −0.585 0.415 GPR55-Tango −0.349 0.090 −0.196 −0.247 0.553 −0.109 −0.143 −0.156 GPR56-Tango −0.265 −0.116 0.166 0.044 0.114 0.099 0.306 0.314 GPR6-Tango 0.338 0.735 0.748 0.560 0.802 0.097 0.609 0.772 GPR61-Tango −0.566 −0.418 −0.243 −0.199 −0.512 −0.743 −0.692 −0.426 GPR62-Tango −0.152 0.766 1.000 0.138 0.000 1.202 −0.152 1.632 GPR63-Tango −0.143 −0.099 −0.078 −0.047 −0.165 0.086 −0.089 −0.054 GPR64-Tango −0.132 −0.129 −0.145 0.046 −0.468 −0.111 −0.092 −0.046 GPR65-Tango −0.266 −0.163 −0.199 −0.163 −0.366 −0.433 −0.399 −0.409 GPR68-Tango −0.532 −0.261 −0.318 −0.381 −0.825 −0.453 −0.372 −0.310 GPR75- Tango −0.385 −0.546 −0.149 −0.125 −0.497 −0.065 −0.103 −0.106 GPR77-Tango −0.494 −0.549 −0.108 −0.525 0.284 −0.280 −0.030 −0.178 GPR78-Tango −0.609 0.018 −0.137 −0.006 −0.257 0.165 −0.111 0.187 GPR82-Tango 0.246 0.415 0.291 0.143 −0.139 −0.043 0.140 0.307 GPR83-Tango −0.520 0.028 −0.190 −0.299 −0.557 −0.222 −0.087 −0.133 GPR84-Tango 0.032 0.149 0.052 0.158 0.128 −0.108 0.296 0.201 GPR85-Tango −0.163 0.034 0.405 0.211 0.826 0.582 −0.056 0.311 GPR87-Tango 0.042 0.392 0.089 0.017 0.331 0.231 0.031 0.097 GPR88-Tango −0.444 −0.598 −0.993 −0.726 −0.240 −0.661 −0.794 −0.661 GPR97-Tango 0.106 0.334 0.061 0.626 0.705 0.734 0.476 1.149 GPRC5A-Tango −0.657 −0.308 −0.243 −0.414 −0.803 −0.492 −0.555 −0.421 GPRC5B-Tango −0.616 −0.293 −0.348 −0.417 −0.699 −0.525 −0.411 −0.367 GPRC5C-Tango −0.222 −0.138 −0.142 −0.106 −0.195 0.035 −0.339 −0.336 GPRC5D-Tango −0.477 −0.125 0.053 −0.007 0.019 −0.148 0.032 −0.110 GPRC6A-Tango −0.494 −0.265 −0.290 −0.434 −0.834 −0.621 −0.519 −0.462 GRM7-Tango −0.399 −0.396 −0.427 −0.425 −0.622 −0.395 −0.422 −0.469 GRM8-Tango −0.077 −0.045 −0.026 −0.125 −0.746 −0.478 0.019 0.095 GRPR-Tango −0.383 −0.383 −0.089 0.309 −0.319 0.349 0.214 0.030 PRESTO-Tango Peptoniphilus Bacteroids Bacteroids Bacteroids Bacteroides Bacteroides Bacteroides Bacteroides with Gifu Medium spp-3# fragilis-1# fragilis-2# fragilis-3# ovatus-1# ovatus-2# ovatus-3# ovatus-4# GPR1-Tango −0.106 −0.726 0.188 −0.871 −0.054 −0.154 −0.633 −0.149 GPR101-Tango 0.070 0.372 0.282 0.381 0.656 0.501 0.579 0.429 GPR110-Tango 0.167 0.402 −0.121 0.162 0.429 0.379 0.312 0.229 GPR111-Tango 1.357 0.080 0.181 −2.392 0.571 0.373 1.181 1.682 GPR113-Tango 0.393 0.377 −0.562 −1.438 0.580 0.346 0.620 0.794 GPR114-Tango 0.165 0.165 −0.700 −0.420 0.325 0.580 0.796 0.446 GPR115-Tango −0.309 −0.173 −0.289 −2.399 0.077 0.282 0.145 −0.208 GPR116-Tango 0.224 0.249 −0.024 −2.192 0.414 0.661 0.479 0.347 GPR119-Tango 0.714 0.941 −0.218 −0.434 1.251 0.816 0.986 1.379 GPR12-Tango 0.224 0.042 0.327 −0.099 0.440 −0.068 0.401 0.212 GPR120-Tango 0.491 0.314 0.745 1.112 1.112 0.491 0.878 0.598 GPR123-Tango 0.099 0.776 −0.399 −0.033 1.150 0.601 0.786 0.510 GPR124-Tango 0.611 0.805 0.850 1.143 0.850 1.165 0.642 0.750 GPR125-Tango −0.364 −0.224 −0.346 −2.458 0.060 0.297 0.080 −0.149 GPR126-Tango 0.071 0.483 0.245 1.061 0.630 1.227 0.570 0.668 GPR132-Tango −0.514 0.383 −1.123 −2.801 0.151 0.192 0.313 −0.259 GPR133-Tango −0.268 −0.081 −0.345 −1.810 0.059 0.193 0.044 −0.127 GPR135-Tango −0.257 0.011 −0.745 −0.585 0.032 −1.011 −0.300 −0.257 GPR141-Tango −0.636 −0.150 0.725 0.364 0.308 0.776 0.522 0.397 GPR142-Tango 1.064 0.563 0.415 0.835 0.713 0.563 0.760 1.136 GPR143-Tango 0.149 0.492 −1.030 −2.396 0.526 0.173 0.660 0.325 GPR144-Tango 0.323 −0.617 −0.319 −2.303 −0.272 −0.367 0.486 0.513 GPR146-Tango −0.234 0.766 0.202 −2.737 0.379 −0.515 1.263 0.766 GPR148-Tango 0.528 −0.339 0.783 −0.967 −0.104 0.097 −0.967 0.274 GPR149-Tango 1.331 1.078 0.953 −0.568 1.213 1.294 0.791 1.674 GPR15-Tango −0.146 0.057 −1.133 −1.474 0.392 −0.146 0.347 −0.506 GPR150-Tango 0.621 0.823 0.387 1.000 0.823 1.254 1.000 1.387 GPR151-Tango 0.616 0.924 0.260 0.410 1.052 0.989 0.722 0.657 GPR152-Tango 0.477 0.521 −0.498 −2.031 0.317 −0.049 0.424 0.555 GPR153-Tango −0.229 0.115 −0.027 −2.151 0.185 0.204 0.220 −0.057 GPR156-Tango 0.298 0.877 0.815 1.121 0.771 0.834 0.616 0.790 GPR157-Tango 0.739 0.953 0.008 −1.846 0.710 0.710 1.082 1.126 GPR158-Tango −0.010 0.741 −0.435 −0.224 0.425 0.787 0.294 0.220 GPR160-Tango −0.157 0.141 −0.810 −0.373 0.241 0.275 0.226 0.034 GPR161-Tango −0.719 −0.278 −0.459 −0.434 −0.013 0.313 0.074 −0.243 GPR162-Tango −0.403 0.193 −0.807 0.128 0.819 −0.062 0.193 0.690 GPR17-Tango −0.053 0.092 −0.024 −2.296 0.271 0.245 0.108 −0.006 GPR171-Tango 0.543 1.192 −1.046 −2.319 1.018 0.677 1.438 0.817 GPR173-Tango 0.274 0.896 −0.104 −0.619 1.066 0.783 1.000 1.246 GPR174-Tango 0.802 0.535 −0.263 −0.963 1.483 0.300 1.214 1.503 GPR18-Tango 1.114 1.184 −0.314 −1.849 1.399 0.816 1.433 1.027 GPR182-Tango −0.184 0.289 0.806 0.130 0.524 0.390 0.474 −0.067 GPR183-Tango 0.076 0.199 −1.177 −0.391 0.444 0.246 0.356 0.497 GPR19-Tango −0.406 0.369 −0.993 −0.441 0.737 0.324 0.771 0.254 GPR20-Tango 0.267 −0.263 −1.054 −4.170 0.395 0.000 0.267 0.267 GPR21-Tango 1.350 1.263 −0.234 −2.737 1.632 0.888 2.036 1.433 GPR22-Tango 0.002 0.735 0.107 0.486 0.661 0.844 0.747 0.474 GPR25-Tango 0.745 1.000 0.491 −1.040 0.491 0.878 0.598 −0.122 GPR26-Tango 0.904 0.791 0.292 −1.354 0.765 0.891 0.928 1.482 GPR27-Tango 1.000 −0.147 0.569 −1.369 −0.495 −0.784 −0.495 0.368 GPR3-Tango −0.277 0.130 1.042 0.197 0.711 0.091 0.762 0.423 GPR31-Tango −0.333 −0.007 −0.370 −0.129 0.184 0.649 0.451 −0.227 GPR32-Tango 1.214 0.223 −0.078 −1.939 0.651 0.537 1.657 1.295 GPR34-Tango 1.130 0.751 0.191 −1.655 0.850 0.683 1.365 1.130 GPR35-Tango 0.280 0.429 −0.105 −2.209 0.479 0.147 0.644 0.533 GPR37-Tango 0.262 0.269 0.422 −1.728 0.456 0.669 0.764 0.288 GPR37L1-Tango 0.416 0.321 −0.185 −1.049 0.705 0.831 0.534 0.431 GPR39-Tango −0.245 0.858 −0.415 −0.508 0.714 0.087 1.000 1.180 GPR4-Tango −0.138 0.164 −0.072 −0.045 0.551 0.577 0.458 0.129 GPR44-Tango 0.872 0.072 0.609 −0.359 1.072 0.439 0.872 −0.075 GPR45-Tango 0.331 0.265 −0.362 −2.528 0.006 −0.431 0.538 1.009 GPR50-Tango 0.657 0.966 −0.409 −1.907 0.900 0.415 1.564 1.050 GPR52-Tango 0.415 0.852 1.377 0.338 1.115 −0.337 0.585 1.010 GPR55-Tango −0.227 0.550 −0.401 −1.858 0.684 0.740 0.628 0.021 GPR56-Tango 0.224 0.463 0.174 0.421 0.719 0.572 0.699 0.519 GPR6-Tango 0.144 1.060 1.051 0.103 1.275 1.070 0.918 0.434 GPR61-Tango −0.313 −0.116 −0.351 −0.484 0.248 0.055 0.074 0.049 GPR62-Tango −1.737 0.138 0.138 −0.737 0.138 0.485 0.766 0.485 GPR63-Tango −0.187 −0.138 −0.112 −2.561 0.145 0.379 0.225 0.099 GPR64-Tango −0.093 −0.453 −0.356 −2.602 −0.074 0.259 −0.184 −0.060 GPR65-Tango −0.388 −0.002 −0.553 −2.106 0.088 0.354 0.065 −0.277 GPR68-Tango −0.244 0.107 −1.270 −2.578 0.128 0.473 0.231 −0.296 GPR75- Tango 0.036 −0.149 −0.722 −0.506 −0.069 −0.335 −0.097 −0.118 GPR77-Tango −0.300 0.022 0.144 −0.178 0.259 0.444 0.360 0.135 GPR78-Tango −0.177 0.689 −0.772 0.525 0.745 0.427 0.312 0.385 GPR82-Tango 0.087 0.316 0.489 0.070 0.503 0.415 0.413 0.018 GPR83-Tango −0.123 0.132 −0.687 −2.626 0.250 0.075 0.429 −0.020 GPR84-Tango −0.035 0.085 0.189 −2.181 0.398 0.400 0.400 −0.033 GPR85-Tango 0.356 0.188 −0.008 −1.807 0.470 0.775 0.817 0.593 GPR87-Tango 0.004 0.237 −0.257 −0.925 0.153 0.588 0.289 0.122 GPR88-Tango −0.119 0.183 −0.444 −0.661 0.432 −0.240 0.451 −0.240 GPR97-Tango 0.031 0.061 0.676 0.860 1.225 0.626 0.911 0.834 GPRC5A-Tango −0.344 0.316 −0.509 −0.541 0.424 0.326 0.436 0.042 GPRC5B-Tango −0.562 0.327 −0.528 −0.626 0.345 0.309 0.358 −0.092 GPRC5C-Tango −0.073 0.375 −0.612 −2.665 0.386 0.351 0.575 0.147 GPRC5D-Tango 0.053 0.108 −0.062 −2.334 0.570 0.399 0.723 0.249 GPRC6A-Tango −0.395 −0.119 −0.702 −2.958 −0.220 −0.107 −0.008 −0.370 GRM7-Tango −0.495 −0.381 −0.783 −2.520 −0.142 0.130 −0.161 −0.369 GRM8-Tango 0.103 0.226 −0.824 −2.174 −0.051 0.440 0.207 −0.109 GRPR-Tango 0.175 0.062 −0.870 −3.356 0.183 −0.179 0.013 0.206 PRESTO-Tango B. B. B. B. B. B. Bacteroides Bacteroides with Gifu Medium ovatus-5# thetao-1# thetao-2# eggerthii Caccae copri uniformis-1# uniformis-2# GPR1-Tango 0.512 −0.284 −0.718 −1.254 −0.564 −0.682 −0.981 −0.335 GPR101-Tango 0.565 0.251 0.224 0.262 0.130 −0.375 0.649 0.081 GPR110-Tango 0.739 0.083 0.271 0.267 0.415 −0.330 0.837 0.189 GPR111-Tango 1.112 0.373 0.373 0.515 0.958 2.305 0.466 0.833 GPR113-Tango 0.104 0.870 −0.258 0.726 −0.007 −0.921 0.418 0.207 GPR114-Tango 0.642 0.399 0.580 0.325 0.077 0.952 0.446 0.515 GPR115-Tango 0.016 −0.095 −0.161 −0.052 −0.135 −0.424 0.126 −0.165 GPR116-Tango 0.253 0.283 0.310 0.367 0.166 0.932 0.303 0.231 GPR119-Tango 0.864 1.029 1.176 0.714 0.660 −0.837 0.766 1.029 GPR12-Tango 0.564 −0.068 −0.436 0.280 0.212 0.014 0.000 −0.039 GPR120-Tango 0.314 1.000 1.217 0.878 0.112 1.697 −0.122 0.598 GPR123-Tango 1.279 0.403 0.158 0.796 0.546 0.214 0.698 −0.069 GPR124-Tango 0.952 0.805 0.165 0.805 0.077 −0.485 0.832 0.515 GPR125-Tango −0.187 −0.205 −0.119 −0.077 −0.290 −0.263 0.035 −0.307 GPR126-Tango 1.516 0.285 −0.115 −0.103 −0.187 0.588 0.891 −0.019 GPR132-Tango 0.326 0.401 0.399 0.479 0.013 −1.115 0.196 −0.068 GPR133-Tango −0.044 −0.025 −0.105 −0.192 −0.211 −0.331 −0.006 −0.146 GPR135-Tango −0.411 −0.441 −0.411 −0.140 −0.521 −1.086 −0.327 −0.411 GPR141-Tango 0.956 0.237 0.014 0.097 −0.301 0.522 0.541 −0.199 GPR142-Tango 0.355 0.713 0.293 0.160 0.112 −0.303 0.791 0.878 GPR143-Tango 0.547 0.580 0.192 0.539 0.370 −0.436 0.455 0.530 GPR144-Tango −0.523 −0.112 −0.367 0.173 0.373 −0.560 −0.319 0.104 GPR146-Tango 0.202 −1.152 0.766 1.104 0.379 −0.862 −0.234 0.485 GPR148-Tango 0.097 0.381 1.066 −0.339 −1.256 0.097 1.066 1.066 GPR149-Tango 0.652 −0.047 0.280 0.523 0.570 0.045 0.849 1.266 GPR15-Tango 0.234 −0.336 −0.662 −0.146 −0.411 −0.336 −0.146 −0.458 GPR150-Tango 0.823 0.107 −2.115 1.107 1.000 −0.241 1.387 1.254 GPR151-Tango 0.531 0.574 −0.011 1.052 0.487 −0.076 0.989 0.361 GPR152-Tango 0.441 0.477 0.430 0.607 0.497 −0.195 0.327 0.516 GPR153-Tango 0.255 0.157 0.200 0.194 −0.146 −0.432 0.191 −0.072 GPR156-Tango 1.322 0.646 0.185 0.424 0.490 1.097 0.858 0.831 GPR157-Tango 0.811 0.453 0.837 1.643 0.508 −0.459 1.167 0.633 GPR158-Tango 0.770 0.656 0.294 0.068 −0.395 −0.517 1.068 0.687 GPR160-Tango 0.436 0.047 0.016 0.015 −0.139 −0.705 0.199 −0.098 GPR161-Tango 0.412 −0.170 −0.025 −0.171 −0.197 −0.435 0.124 −0.391 GPR162-Tango 0.372 0.860 0.409 0.900 0.315 −0.941 0.012 −0.469 GPR17-Tango 0.276 −0.097 −0.181 0.010 −0.051 −0.141 −0.049 0.033 GPR171-Tango 0.631 0.576 0.685 1.162 0.600 −0.404 0.929 0.564 GPR173-Tango 0.528 0.896 0.661 0.896 0.896 −0.967 0.274 0.381 GPR174-Tango 0.509 0.509 0.429 0.509 1.054 −0.453 0.834 0.429 GPR18-Tango 0.975 0.867 0.499 1.190 1.325 −0.104 0.975 0.949 GPR182-Tango 0.197 0.323 0.085 0.153 −0.116 −0.401 0.480 0.089 GPR183-Tango 0.340 0.217 0.388 0.773 0.577 −0.418 0.199 −0.038 GPR19-Tango 0.539 0.163 0.260 0.216 −0.118 −0.923 0.598 0.195 GPR20-Tango 0.800 −0.329 −1.433 −1.231 0.152 −1.948 0.152 0.333 GPR21-Tango 1.104 1.000 1.170 1.585 1.104 −0.515 1.433 1.000 GPR22-Tango 1.129 0.692 −0.081 0.136 0.180 −0.346 0.786 0.296 GPR25-Tango 1.000 1.463 0.112 0.598 −0.122 −2.624 1.463 1.813 GPR26-Tango 0.904 0.450 0.461 0.509 0.683 0.658 0.605 0.710 GPR27-Tango −0.495 −0.147 0.133 0.133 0.368 0.500 0.368 −0.495 GPR3-Tango 0.507 0.344 0.295 0.284 0.033 −0.978 0.304 0.300 GPR31-Tango 1.036 0.118 −0.730 −0.007 −0.279 0.563 0.621 0.036 GPR32-Tango 1.564 0.828 0.625 0.939 0.932 0.720 1.191 1.445 GPR34-Tango 1.049 0.421 1.113 1.239 1.084 0.545 1.067 0.659 GPR35-Tango 0.784 0.269 0.472 0.569 0.398 −0.889 0.475 0.648 GPR37-Tango 0.565 0.252 0.191 0.398 0.183 −0.394 0.487 0.559 GPR37L1-Tango 0.367 0.091 0.063 0.399 0.374 −0.020 0.321 0.484 GPR39-Tango 0.672 0.555 0.629 0.409 0.714 0.150 0.672 0.629 GPR4-Tango 0.491 0.221 0.048 0.164 0.062 −0.601 0.440 0.414 GPR44-Tango 0.950 0.790 0.072 0.327 −0.075 0.544 1.072 0.439 GPR45-Tango 0.750 −0.303 −0.431 −0.078 −0.041 −0.641 0.250 0.101 GPR50-Tango 0.882 0.936 0.291 0.983 0.864 −0.440 0.794 1.077 GPR52-Tango 1.115 0.239 −0.585 −0.082 −0.020 0.980 0.040 0.239 GPR55-Tango 0.676 0.392 0.323 0.640 0.241 0.267 0.342 0.005 GPR56-Tango 1.220 0.646 0.278 −0.066 0.349 0.725 1.103 0.327 GPR6-Tango 0.752 0.329 −0.115 0.575 0.306 −0.463 0.434 0.602 GPR61-Tango 0.183 −0.050 −0.343 −0.006 −0.172 −0.743 0.097 −0.537 GPR62-Tango 1.202 1.202 1.000 1.202 −0.737 −0.737 −0.152 −0.737 GPR63-Tango 0.138 −0.052 −0.052 0.066 −0.429 −0.171 −0.035 −0.077 GPR64-Tango −0.290 −0.440 −0.252 −0.108 −0.350 −0.009 −0.132 −0.189 GPR65-Tango −0.015 0.095 −0.074 0.104 −0.133 −0.613 0.042 −0.229 GPR68-Tango −0.070 0.034 0.183 0.224 0.034 −1.656 0.148 0.174 GPR75- Tango 0.142 −0.219 −0.416 −0.072 0.057 −0.215 −0.346 0.181 GPR77-Tango 0.571 0.151 −0.274 −0.018 −0.139 −0.393 −0.004 0.343 GPR78-Tango 0.757 0.347 0.018 −0.039 −0.431 −0.609 0.486 0.245 GPR82-Tango 0.379 0.042 −0.081 0.450 0.093 −0.174 0.513 −0.224 GPR83-Tango 0.322 0.304 0.267 0.488 0.241 −0.199 0.362 0.205 GPR84-Tango 0.199 0.063 0.043 0.057 −0.102 0.166 0.067 0.127 GPR85-Tango 0.866 0.620 0.522 0.908 0.843 0.369 0.600 0.712 GPR87-Tango 0.324 0.137 −0.110 0.075 0.080 −0.781 0.146 0.294 GPR88-Tango 0.402 0.007 −0.119 −0.339 −0.240 0.451 −0.391 −0.539 GPR97-Tango 1.436 0.499 0.476 −0.016 0.297 0.656 1.091 0.564 GPRC5A-Tango 0.917 0.163 −0.327 0.172 −0.274 0.070 0.591 −0.135 GPRC5B-Tango 0.711 0.107 −0.152 −0.108 −0.064 −0.758 0.382 −0.215 GPRC5C-Tango 0.523 0.359 0.187 0.476 0.271 −0.342 0.299 0.062 GPRC5D-Tango 0.495 0.095 0.078 0.091 0.078 −0.262 0.419 0.014 GPRC6A-Tango 0.068 −0.087 −0.002 −0.020 −0.324 −0.413 0.038 −0.249 GRM7-Tango −0.105 −0.057 −0.122 −0.093 −0.326 −0.735 −0.463 −0.225 GRM8-Tango 0.081 0.075 0.184 0.149 0.218 1.132 0.292 0.365 GRPR-Tango 0.170 −0.004 −0.544 −0.394 0.183 −0.519 −0.190 0.633 PRESTO-Tango Bacteroides Bacteroides Bacteroides Bacteroides Parabacteroides Parabacteroides Fusobacterium Fusobacterium with Gifu Medium uniformis-3# UC-1# UC-2# UC-3# distasonis-1# distasonis-2# spp-1# spp-2# GPR1-Tango −0.906 0.160 −0.154 0.128 −0.073 −0.645 −0.817 −0.344 GPR101-Tango 0.302 0.870 0.231 0.191 0.295 0.164 −0.587 0.168 GPR110-Tango 0.316 0.495 0.283 0.489 0.267 −0.025 −0.870 −0.100 GPR111-Tango 0.958 1.594 0.915 1.162 0.786 0.405 −0.505 1.181 GPR113-Tango 0.393 0.681 0.558 0.594 0.668 −0.242 −1.332 0.478 GPR114-Tango 0.165 0.515 0.446 0.702 0.796 0.580 −2.338 −0.082 GPR115-Tango −0.090 0.142 −0.027 0.088 −0.141 −0.265 −0.958 −0.304 GPR116-Tango 0.388 0.482 0.322 0.446 0.222 0.253 −0.359 0.077 GPR119-Tango 0.766 0.444 0.941 0.864 0.816 0.766 1.614 1.251 GPR12-Tango −0.188 −0.140 0.368 0.338 0.273 0.106 −1.529 −0.455 GPR120-Tango −0.402 1.000 0.112 1.623 0.314 0.598 −0.402 −0.402 GPR123-Tango 0.142 0.716 1.017 0.489 0.390 0.312 −1.588 0.126 GPR124-Tango 0.000 0.547 0.458 0.547 0.287 0.287 1.469 0.287 GPR125-Tango −0.163 0.078 −0.190 −0.085 −0.077 −0.300 −0.311 −0.071 GPR126-Tango 0.359 0.346 0.384 0.071 0.236 −0.295 0.040 0.165 GPR132-Tango −0.188 −0.184 0.157 0.178 −0.349 −0.145 −1.243 −0.607 GPR133-Tango −0.112 0.185 −0.081 0.027 −0.029 −0.336 −0.724 −0.230 GPR135-Tango −0.926 −0.156 −0.026 −0.521 −0.490 −0.521 −1.807 −1.132 GPR141-Tango −0.825 0.200 0.056 0.308 −0.825 −0.104 −0.825 −0.508 GPR142-Tango 0.760 0.878 0.791 0.713 0.760 0.112 −0.303 0.509 GPR143-Tango 0.029 0.322 0.375 0.486 −0.008 0.135 −1.874 −0.225 GPR144-Tango 0.373 0.196 0.323 0.292 −0.242 −0.417 1.743 0.557 GPR146-Tango 0.000 0.632 0.202 −1.152 0.202 −1.152 −1.737 0.766 GPR148-Tango 0.783 0.783 0.661 0.274 −0.104 0.097 1.000 0.661 GPR149-Tango 1.266 1.034 1.280 0.811 1.227 0.868 1.126 1.884 GPR15-Tango −0.171 −0.293 0.034 −0.211 −0.381 −0.556 −2.265 −0.699 GPR150-Tango 0.621 0.621 −0.241 0.823 0.621 −1.115 0.387 0.387 GPR151-Tango 0.052 0.797 0.574 0.574 −0.343 0.260 −0.672 0.052 GPR152-Tango 0.205 −0.049 0.602 0.714 0.404 0.629 −1.233 −0.139 GPR153-Tango 0.079 0.132 0.138 0.141 0.021 −0.085 −1.651 −0.161 GPR156-Tango 0.666 0.931 0.801 0.764 0.662 0.023 0.670 0.600 GPR157-Tango 0.937 0.311 1.167 1.126 0.862 0.888 0.126 0.653 GPR158-Tango 0.504 0.504 0.245 0.403 0.123 −0.259 0.096 0.245 GPR160-Tango −0.326 0.357 0.025 −0.089 −0.102 −0.462 −1.161 −0.366 GPR161-Tango −0.357 0.075 −0.261 −0.260 −0.626 −0.601 −1.329 −0.708 GPR162-Tango 0.315 0.613 0.255 0.255 −0.469 −0.140 −2.435 0.059 GPR17-Tango −0.004 0.378 0.152 0.215 0.133 −0.151 −1.250 0.114 GPR171-Tango 0.432 1.042 0.769 0.769 0.913 0.354 −1.882 0.838 GPR173-Tango 1.407 1.000 1.159 −0.104 0.896 0.783 −2.841 0.097 GPR174-Tango 0.895 0.429 0.645 1.198 1.072 1.098 −0.963 0.387 GPR18-Tango 1.084 1.071 0.543 1.120 1.014 0.499 −0.808 1.229 GPR182-Tango 0.021 0.639 0.065 0.304 0.182 −0.238 −0.600 −0.042 GPR183-Tango −0.446 0.356 0.127 0.246 0.095 0.199 −1.374 −0.418 GPR19-Tango 0.238 0.323 0.305 0.535 −0.103 −0.332 −1.182 −0.396 GPR20-Tango −0.667 −0.329 0.333 −0.170 −0.170 0.000 −1.667 −0.433 GPR21-Tango 1.511 1.000 0.485 1.433 0.379 0.888 −1.152 1.888 GPR22-Tango 0.255 0.569 0.576 0.398 0.180 −0.234 0.142 −0.019 GPR25-Tango 1.112 0.598 1.217 0.878 1.745 1.217 −0.750 1.463 GPR26-Tango 1.012 0.799 0.978 1.117 1.167 0.665 −0.124 1.031 GPR27-Tango −0.495 −0.495 −0.495 0.853 0.133 −0.495 −1.369 0.368 GPR3-Tango −0.125 0.587 0.105 0.170 0.211 −0.086 −1.328 −0.164 GPR31-Tango −0.227 0.847 −0.129 0.386 −0.427 −0.804 −1.682 −0.804 GPR32-Tango 1.045 1.231 1.557 1.571 1.308 0.872 −2.099 0.388 GPR34-Tango 0.520 0.765 1.270 0.904 0.871 0.476 −1.109 0.924 GPR35-Tango 0.571 0.458 0.849 0.788 0.544 0.317 −1.840 0.201 GPR37-Tango 0.329 0.510 0.696 0.523 0.323 0.239 −0.685 0.299 GPR37L1-Tango 0.372 0.780 0.525 0.755 0.287 0.081 0.146 0.403 GPR39-Tango 0.304 0.672 0.267 0.304 0.672 0.459 0.087 0.629 GPR4-Tango 0.136 0.352 0.426 0.406 0.128 −0.190 −1.001 −0.459 GPR44-Tango 0.439 0.205 0.544 0.872 0.702 −0.075 −2.298 0.205 GPR45-Tango 0.149 0.206 0.468 0.275 0.275 −0.059 −1.282 −0.339 GPR50-Tango 0.832 0.291 1.000 0.657 0.774 0.389 −0.239 0.882 GPR52-Tango 0.544 0.830 0.188 0.761 −0.082 −0.216 −0.263 −0.337 GPR55-Tango −0.221 0.600 0.383 0.628 0.204 −0.232 −0.477 −0.017 GPR56-Tango 0.317 0.500 0.852 0.437 0.631 0.166 −0.786 −0.116 GPR6-Tango 0.065 0.825 0.226 0.443 0.582 0.443 −0.859 0.306 GPR61-Tango −0.335 −0.123 0.019 −0.484 −0.267 −0.267 −1.703 −0.296 GPR62-Tango 0.766 1.000 1.485 1.485 −0.152 0.485 −0.737 0.138 GPR63-Tango 0.000 0.369 −0.018 −0.066 −0.152 −0.341 −0.927 0.000 GPR64-Tango −0.270 −0.021 −0.087 −0.245 −0.346 −0.254 −1.869 −0.019 GPR65-Tango −0.183 0.033 −0.117 0.103 −0.155 −0.112 −0.974 −0.333 GPR68-Tango −0.056 0.044 0.100 0.165 −0.063 −0.139 −1.255 −0.396 GPR75- Tango 0.007 −0.113 0.220 −0.127 −0.192 −0.114 −1.559 −0.823 GPR77-Tango −0.452 0.346 0.578 −0.004 0.079 −0.219 −1.423 −0.736 GPR78-Tango −0.257 0.682 −0.177 −0.018 −0.257 0.158 −1.856 −0.018 GPR82-Tango −0.182 0.368 0.302 0.223 0.299 −0.074 −1.033 −0.060 GPR83-Tango −0.011 0.265 0.360 0.378 −0.235 0.172 −1.772 −0.533 GPR84-Tango 0.028 0.392 0.265 0.164 0.366 −0.241 −1.468 0.092 GPR85-Tango 0.344 0.459 0.650 0.784 0.750 0.412 −0.961 0.079 GPR87-Tango −0.040 0.301 0.219 0.280 0.299 −0.075 −1.398 −0.054 GPR88-Tango −0.339 0.307 −0.726 −0.841 −0.391 −0.481 −2.225 −1.256 GPR97-Tango 0.499 1.091 0.960 0.676 0.789 0.334 −0.257 0.271 GPRC5A-Tango 0.067 0.288 0.060 0.267 −0.444 −0.478 −1.348 −0.723 GPRC5B-Tango −0.186 0.247 −0.097 0.050 −0.279 −0.389 −1.165 −0.798 GPRC5C-Tango 0.423 −0.030 0.351 0.308 0.010 0.117 −1.418 −0.036 GPRC5D-Tango 0.294 0.419 0.458 0.181 0.277 −0.193 −1.270 0.273 GPRC6A-Tango −0.253 −0.044 −0.204 0.013 −0.375 −0.340 −1.818 −0.705 GRM7-Tango −0.274 −0.245 −0.161 −0.049 −0.283 −0.267 −0.881 −0.498 GRM8-Tango 0.089 0.086 0.089 0.469 −0.136 −0.055 −1.314 −0.529 GRPR-Tango 0.290 −0.532 0.567 0.214 0.249 −0.248 −2.312 0.036 Bifidobac- Bifidobac- PRESTO-Tango Collinsella Collinsella Collinsella Collinsella Collinsella Collinsella terium terium with Gifu Medium aerofaciens-1# aerofaciens-2# aerofaciens-3# stercoris-1# stercoris-2# stercoris-3# spp UC GPR1-Tango 1.226 1.484 0.882 0.184 0.052 0.267 −0.335 −0.215 GPR101-Tango 1.008 0.900 0.877 0.232 0.205 0.221 −0.251 0.330 GPR110-Tango 1.126 1.135 0.851 0.489 0.211 0.308 −1.123 −0.053 GPR111-Tango 0.571 0.720 1.246 0.252 0.599 0.373 0.571 0.373 GPR113-Tango 0.104 0.448 0.303 −0.542 −0.307 −0.112 −0.384 0.487 GPR114-Tango 0.247 0.515 0.796 −0.298 0.399 −0.420 0.247 0.399 GPR115-Tango 0.324 0.358 0.231 0.068 −0.081 −0.167 −0.128 −0.156 GPR116-Tango 0.672 0.663 0.587 0.115 0.344 0.168 −0.704 0.296 GPR119-Tango 1.214 1.287 0.816 −0.059 0.379 0.506 −0.837 0.379 GPR12-Tango 0.574 0.679 0.408 0.292 0.338 0.280 −1.277 −0.743 GPR120-Tango 0.491 0.598 0.314 1.112 0.878 0.878 0.112 0.491 GPR123-Tango 1.596 1.732 1.365 0.214 0.099 0.214 0.982 −0.316 GPR124-Tango 1.441 1.611 1.487 −0.048 0.350 0.122 −1.554 0.515 GPR125-Tango 0.287 0.247 0.105 −0.042 −0.126 −0.129 0.466 −0.010 GPR126-Tango 1.840 1.659 1.798 0.112 0.040 0.071 −0.654 0.416 GPR132-Tango 0.208 0.301 0.008 −0.175 −0.094 −0.209 −2.009 −0.274 GPR133-Tango 0.281 0.293 0.080 0.039 −0.077 0.144 −0.550 −0.160 GPR135-Tango 0.255 0.290 0.205 −0.490 0.074 −0.327 −0.846 −1.618 GPR141-Tango 1.110 0.776 0.364 −0.301 0.136 −0.354 −0.073 −0.986 GPR142-Tango 1.228 1.463 1.261 0.509 0.472 0.791 1.136 0.228 GPR143-Tango 0.541 0.769 0.492 0.302 0.532 0.414 −0.265 −0.407 GPR144-Tango 0.139 0.031 0.173 0.104 0.031 0.031 −0.154 −0.560 GPR146-Tango −0.234 0.379 1.263 0.766 0.379 0.485 −0.862 −0.862 GPR148-Tango 0.896 0.896 1.066 0.274 −0.104 0.381 −0.339 0.274 GPR149-Tango 1.398 1.503 1.445 0.380 0.652 0.546 1.674 0.203 GPR15-Tango 0.861 0.822 0.707 −0.381 0.000 0.000 −1.878 −1.381 GPR150-Tango 0.107 0.387 0.823 −2.115 0.387 0.387 −2.115 −2.115 GPR151-Tango 1.260 1.159 0.797 0.111 0.487 0.169 −0.076 −0.513 GPR152-Tango 0.531 0.617 0.579 0.241 0.356 0.404 1.032 −0.358 GPR153-Tango 0.303 0.525 0.161 0.143 0.117 0.174 −0.223 −0.215 GPR156-Tango 1.327 1.457 1.397 0.345 0.677 0.783 −0.609 −0.858 GPR157-Tango 0.888 1.598 1.541 0.384 0.475 −0.039 −0.243 0.082 GPR158-Tango 1.326 1.194 0.578 −0.071 0.039 −0.259 −0.780 −0.102 GPR160-Tango 0.681 0.729 0.613 0.077 0.152 0.086 −1.803 −0.416 GPR161-Tango 0.731 0.967 0.586 −0.105 −0.038 −0.149 −1.908 −0.255 GPR162-Tango 0.660 0.193 0.565 0.012 0.372 −0.062 −1.371 −1.087 GPR17-Tango 0.526 0.443 0.475 0.087 0.033 −0.007 0.181 −0.215 GPR171-Tango 0.957 1.042 0.982 0.445 0.858 0.779 0.009 −0.136 GPR173-Tango 1.000 0.896 1.000 0.783 −0.104 0.274 0.896 1.066 GPR174-Tango 1.000 1.645 1.149 0.645 0.864 0.469 −0.136 −0.076 GPR18-Tango 1.303 1.394 1.199 1.051 1.143 1.114 0.239 0.262 GPR182-Tango 0.657 0.541 0.146 0.376 −0.043 −0.142 −1.356 −0.154 GPR183-Tango 0.340 0.540 0.280 0.114 −0.139 0.340 −1.017 −0.709 GPR19-Tango 1.246 1.346 0.977 0.541 0.639 0.093 −1.689 −0.631 GPR20-Tango 0.395 0.512 0.333 −0.545 −0.263 0.333 −1.054 −1.667 GPR21-Tango 1.170 1.982 1.585 1.433 1.263 1.104 −0.234 0.485 GPR22-Tango 1.447 1.360 1.183 0.377 0.070 0.195 0.583 0.273 GPR25-Tango 0.598 1.282 1.463 1.217 0.314 −0.402 −0.122 0.491 GPR26-Tango 0.822 1.296 0.838 0.765 0.808 0.640 0.716 0.335 GPR27-Tango 1.000 2.133 1.538 −2.369 −0.495 0.133 0.569 0.133 GPR3-Tango 0.611 0.682 0.842 0.003 −0.034 0.266 −0.400 −0.136 GPR31-Tango 1.341 1.240 1.057 −0.333 −0.487 −0.227 −0.882 −0.427 GPR32-Tango 1.686 1.539 1.571 0.472 1.431 0.939 −0.684 −0.914 GPR34-Tango 1.326 1.255 1.520 0.570 0.924 1.067 −0.714 −0.405 GPR35-Tango 1.102 1.211 0.798 0.575 0.505 0.523 −0.355 −0.299 GPR37-Tango 0.759 0.851 0.713 0.531 0.314 0.262 −0.666 0.268 GPR37L1-Tango 0.967 0.967 0.802 0.487 0.727 0.776 0.287 0.132 GPR39-Tango 1.087 0.755 0.714 1.000 0.150 0.672 0.555 −0.046 GPR4-Tango 0.807 0.908 0.834 0.518 0.082 0.009 −0.937 −0.101 GPR44-Tango 0.950 1.072 0.327 0.205 0.609 −0.561 −0.561 −0.182 GPR45-Tango 1.049 1.071 0.863 0.513 0.317 0.468 −2.000 −1.059 GPR50-Tango 1.263 1.050 1.225 0.553 0.919 0.851 −0.365 −0.409 GPR52-Tango 1.030 1.503 0.885 0.544 0.544 −0.263 0.097 0.134 GPR55-Tango 0.589 0.738 0.692 0.267 0.121 0.285 −0.267 0.072 GPR56-Tango 1.424 1.326 1.276 0.394 0.492 0.477 −1.320 0.183 GPR6-Tango 1.750 1.203 0.983 0.844 0.504 0.443 −0.659 −0.819 GPR61-Tango 0.704 0.571 0.216 −0.199 −0.329 −0.111 −0.819 −0.418 GPR62-Tango 0.766 0.766 0.766 −0.152 1.000 0.766 0.000 0.138 GPR63-Tango 0.461 0.368 0.256 0.033 0.103 0.011 −1.029 −0.092 GPR64-Tango 0.333 0.198 0.123 −0.272 −0.224 −0.229 0.073 −0.149 GPR65-Tango 0.314 0.411 0.150 −0.031 −0.130 0.069 −0.254 0.018 GPR68-Tango 0.706 0.676 0.463 0.004 0.159 −0.014 −0.150 −0.261 GPR75- Tango 0.532 0.651 0.503 −0.472 0.199 −0.067 −1.447 −0.926 GPR77-Tango 1.229 0.741 0.845 0.312 0.340 0.291 −1.423 −0.111 GPR78-Tango 0.331 0.731 0.158 −0.064 −0.177 0.061 −0.458 0.256 GPR82-Tango 0.789 1.098 0.832 0.220 −0.068 0.015 −1.410 −0.174 GPR83-Tango 0.662 0.750 0.515 0.241 0.145 0.092 −1.979 −0.642 GPR84-Tango 0.482 0.619 0.476 0.215 0.171 −0.052 −0.021 0.174 GPR85-Tango 0.731 0.960 0.722 0.356 0.447 0.630 −0.652 0.023 GPR87-Tango 0.457 0.601 0.582 0.213 0.294 0.258 0.220 0.007 GPR88-Tango 0.636 0.799 0.636 −0.539 −0.163 0.147 −1.941 −0.841 GPR97-Tango 2.293 2.076 1.825 0.789 0.789 0.960 1.211 −0.501 GPRC5A-Tango 0.992 1.146 0.950 −0.159 0.004 −0.090 0.137 −0.421 GPRC5B-Tango 1.048 1.194 0.988 0.369 0.097 0.147 −0.498 −0.699 GPRC5C-Tango 0.949 0.666 0.409 0.018 0.253 0.325 −1.527 −0.741 GPRC5D-Tango 0.681 0.904 0.819 0.614 0.369 0.239 0.639 −0.245 GPRC6A-Tango 0.137 0.165 −0.016 −0.008 −0.213 −0.082 −1.953 −0.369 GRM7-Tango 0.409 0.334 0.141 −0.267 −0.198 −0.367 −0.783 −0.445 GRM8-Tango 0.587 0.759 0.371 −0.081 0.123 0.019 −0.145 −0.153 GRPR-Tango 0.363 0.590 0.606 0.138 0.342 0.363 −1.326 −0.930 PRESTO-Tango Bifidobacterium Bifidobacterium Bifidobacterium Morganella Morganella Morganella Morganella Morganella with Gifu Medium adolescentis-1# adolescentis-2# adolescentis-3# spp-1# spp-2# spp-3# spp-4# spp-5# GPR1-Tango 0.431 0.283 −0.823 0.374 0.509 0.594 0.487 0.573 GPR101-Tango 0.465 0.420 0.332 0.881 0.359 0.553 0.503 0.459 GPR110-Tango 0.203 0.130 0.130 0.736 −0.062 0.340 0.300 0.287 GPR111-Tango 0.308 0.543 0.436 0.695 0.856 1.000 1.216 1.420 GPR113-Tango −0.048 −0.195 1.101 −0.149 0.007 0.007 0.135 0.681 GPR114-Tango 0.325 0.446 −0.082 −0.082 0.399 0.580 0.902 1.077 GPR115-Tango −0.138 −0.145 −0.024 −0.300 −0.286 −0.141 −0.171 −0.227 GPR116-Tango 0.407 0.476 0.365 0.685 0.664 0.576 0.641 0.674 GPR119-Tango 0.816 −0.152 0.566 0.506 0.623 0.379 1.176 0.941 GPR12-Tango 0.401 0.924 0.554 0.590 0.408 0.760 0.419 0.751 GPR120-Tango 0.491 0.745 0.598 0.878 1.623 0.112 1.112 0.598 GPR123-Tango 1.702 1.596 0.958 0.312 −0.316 −0.033 0.110 0.259 GPR124-Tango 0.805 1.791 0.927 0.611 0.312 0.312 0.312 0.077 GPR125-Tango −0.007 −0.197 0.043 0.041 −0.326 −0.159 −0.271 −0.277 GPR126-Tango 0.259 0.678 0.384 0.858 0.715 0.698 0.769 0.882 GPR132-Tango 0.098 −0.005 −0.394 −0.145 −0.311 −0.301 −0.327 −0.273 GPR133-Tango −0.055 0.080 −0.190 −0.033 −0.134 −0.122 −0.095 −0.133 GPR135-Tango −0.460 −0.181 −0.327 −0.521 −0.885 −0.411 −0.181 −0.490 GPR141-Tango 0.776 1.255 0.571 0.397 −0.391 0.461 0.461 0.541 GPR142-Tango 1.026 1.000 0.112 0.760 −0.368 0.791 0.355 −0.368 GPR143-Tango 0.494 0.219 −0.023 −0.008 −0.149 −0.077 0.135 0.173 GPR144-Tango 0.139 −0.154 0.173 −0.367 −0.417 −0.072 0.486 0.608 GPR146-Tango −0.234 0.888 −0.234 0.000 0.379 0.485 1.104 0.485 GPR148-Tango 0.381 −0.104 0.274 1.066 0.097 −0.339 0.661 0.528 GPR149-Tango 2.438 2.645 2.333 1.034 1.308 1.017 0.363 0.791 GPR15-Tango −0.506 0.034 0.089 −0.458 −0.878 −0.293 −0.816 0.320 GPR150-Tango 0.621 0.387 1.000 0.823 0.387 0.387 −0.241 −0.241 GPR151-Tango 0.760 1.472 1.595 0.531 −0.343 −0.011 0.260 −0.343 GPR152-Tango 0.327 0.925 0.612 −0.348 −0.139 0.097 −0.110 0.057 GPR153-Tango −0.013 0.156 −0.001 0.204 −0.016 −0.290 0.026 0.004 GPR156-Tango 0.904 0.939 0.939 1.316 1.373 1.424 1.380 1.243 GPR157-Tango 0.311 0.384 0.248 0.311 0.710 0.419 1.000 1.221 GPR158-Tango 1.723 1.942 1.015 0.310 −0.605 −0.190 −0.295 0.194 GPR160-Tango 0.150 0.393 0.254 −0.260 −0.405 −0.207 −0.328 −0.145 GPR161-Tango −0.284 0.015 −0.348 0.062 −0.154 −0.192 −0.250 0.000 GPR162-Tango 1.036 0.409 0.059 −0.222 0.193 −0.573 −0.140 −1.087 GPR17-Tango 0.307 0.121 −0.228 0.307 0.451 0.352 0.288 0.215 GPR171-Tango 0.696 1.059 0.666 −0.097 0.493 0.817 1.018 0.991 GPR173-Tango −0.104 1.159 0.274 1.066 1.246 0.783 1.066 0.896 GPR174-Tango 1.054 0.737 0.222 −0.263 −0.379 0.509 0.509 0.509 GPR18-Tango 0.686 0.568 0.930 0.423 0.385 0.816 1.107 1.047 GPR182-Tango 0.705 0.722 0.777 0.715 0.282 0.738 0.718 0.639 GPR183-Tango −0.038 0.163 0.163 −0.161 −0.551 −0.612 −0.184 −0.017 GPR19-Tango 0.823 1.225 0.837 −0.118 −0.443 −0.184 −0.297 −0.095 GPR20-Tango 0.199 −0.801 −0.667 −0.433 −0.329 0.395 0.512 0.455 GPR21-Tango 1.585 1.104 1.170 0.632 1.263 1.766 2.087 1.982 GPR22-Tango 0.521 0.782 0.811 0.107 −0.424 0.195 0.097 0.296 GPR25-Tango 1.813 −0.122 −0.402 0.598 1.813 1.217 1.000 1.545 GPR26-Tango 1.045 0.760 1.384 0.605 1.019 1.245 1.085 1.373 GPR27-Tango −0.784 −0.495 0.133 0.569 0.133 −0.495 0.746 0.569 GPR3-Tango 0.266 0.105 −0.097 0.486 0.456 0.427 0.464 0.561 GPR31-Tango 0.933 0.258 −0.082 0.543 −0.333 0.676 0.649 1.276 GPR32-Tango −0.099 −0.023 −0.414 0.663 0.638 1.500 1.621 2.128 GPR34-Tango 0.829 0.787 0.520 0.476 1.444 0.962 1.640 1.444 GPR35-Tango −0.394 −0.503 0.048 0.095 0.361 0.554 0.724 0.460 GPR37-Tango 0.506 0.465 0.505 0.594 0.491 0.729 0.612 0.518 GPR37L1-Tango 0.643 0.791 0.527 0.264 0.178 0.164 0.432 0.290 GPR39-Tango 0.672 0.358 1.087 0.672 0.459 0.209 0.267 0.629 GPR4-Tango 0.531 0.752 0.392 0.276 −0.197 0.439 0.251 0.541 GPR44-Tango 0.205 2.236 1.527 −0.075 0.072 1.327 0.702 0.205 GPR45-Tango 0.074 0.275 −0.186 0.165 0.006 0.932 1.009 0.657 GPR50-Tango 1.314 1.000 0.851 −0.365 0.319 0.155 0.774 1.034 GPR52-Tango 1.214 0.688 1.377 0.761 0.239 0.688 0.830 0.624 GPR55-Tango 0.526 0.231 0.582 0.306 0.555 0.458 0.428 0.396 GPR56-Tango 0.372 0.568 0.725 0.522 0.418 0.884 0.824 0.852 GPR6-Tango 1.266 1.185 0.994 1.309 1.065 1.457 0.951 1.115 GPR61-Tango 0.171 −0.304 −0.026 −0.603 −0.623 −0.215 −0.682 −0.603 GPR62-Tango 2.104 1.632 1.000 1.202 −0.737 −0.152 0.766 0.485 GPR63-Tango 0.646 0.625 −0.237 0.063 −0.023 0.093 0.008 −0.041 GPR64-Tango −0.254 −0.063 −0.030 0.155 −0.041 0.001 −0.152 −0.038 GPR65-Tango −0.136 0.168 −0.284 −0.130 −0.185 −0.228 −0.291 −0.349 GPR68-Tango 0.120 0.216 −0.104 −0.453 −0.702 −0.347 −0.428 −0.358 GPR75- Tango −0.122 0.217 −0.035 −0.675 −0.360 −0.383 −0.263 0.131 GPR77-Tango 0.774 0.645 0.543 −0.071 −0.198 0.451 0.192 0.871 GPR78-Tango 0.427 0.366 0.906 −0.187 −0.039 0.208 −0.006 −0.064 GPR82-Tango 0.179 0.625 −0.491 0.895 0.206 0.717 0.672 0.657 GPR83-Tango −0.133 −0.461 −0.728 −0.262 −0.190 −0.209 −0.066 −0.215 GPR84-Tango 0.018 0.144 0.145 0.255 0.359 0.272 0.555 0.296 GPR85-Tango 0.470 0.424 0.034 0.731 0.784 0.722 0.765 0.696 GPR87-Tango 0.089 0.219 0.095 0.169 −0.041 0.173 0.351 0.325 GPR88-Tango 0.565 0.402 0.565 −0.289 −0.598 −0.194 0.565 0.481 GPR97-Tango 1.023 1.149 1.651 0.334 1.170 1.636 1.371 1.454 GPRC5A-Tango 0.803 0.585 0.310 0.004 −0.251 0.057 −0.060 −0.073 GPRC5B-Tango 0.103 0.727 0.615 0.005 −0.465 0.001 −0.316 −0.245 GPRC5C-Tango −0.181 −0.418 −0.524 −0.387 −0.194 0.022 −0.104 −0.268 GPRC5D-Tango 0.158 0.472 0.910 −0.014 −0.352 0.158 0.128 0.164 GPRC6A-Tango −0.084 −0.192 −1.129 −0.303 −0.546 −0.398 −0.412 −0.457 GRM7-Tango −0.107 −0.073 −0.311 −0.703 −0.572 −0.589 −0.367 −0.528 GRM8-Tango −0.042 0.052 −0.149 0.297 0.269 0.176 0.267 0.272 GRPR-Tango −0.566 −0.947 −1.371 −0.886 −0.153 −0.383 0.363 0.170 PRESTO-Tango Morganella (Enterobacteria) (Enterobacteria) (Enterobacteria) (Enterobacteria) (Enterobacteria) with Gifu Medium spp-6# UC/UC-1# UC/UC-2# UC/UC-3# UC/UC-4# UC/UC-5# GPR1-Tango 0.361 0.727 0.371 0.632 0.406 −2.054 GPR101-Tango 0.224 0.419 0.304 0.405 0.217 −1.395 GPR110-Tango 0.415 −0.316 −0.341 0.267 −0.117 −1.813 GPR111-Tango 1.461 −0.085 −0.206 0.571 0.599 −2.014 GPR113-Tango −0.090 0.303 0.085 −0.384 0.236 −2.069 GPR114-Tango 0.642 −0.082 −0.700 0.399 0.247 −2.048 GPR115-Tango −0.351 −0.130 −0.336 0.025 0.063 −2.158 GPR116-Tango 0.490 0.394 −0.028 0.346 0.175 −1.862 GPR119-Tango 1.070 −0.322 −0.556 0.111 0.310 1.322 GPR12-Tango 0.661 0.889 0.292 0.751 0.924 −1.093 GPR120-Tango 0.314 0.112 1.112 0.745 0.745 −0.402 GPR123-Tango −0.069 −0.177 −0.422 0.416 0.082 −2.039 GPR124-Tango 0.458 0.047 0.350 0.206 0.312 −1.082 GPR125-Tango −0.449 −0.131 −0.317 −0.145 −0.134 −2.505 GPR126-Tango 0.939 0.333 −0.035 0.359 0.208 −0.629 GPR132-Tango −0.296 −1.089 −1.117 −0.651 −0.538 −2.466 GPR133-Tango −0.387 0.026 −0.221 −0.004 0.134 −2.238 GPR135-Tango −1.231 −0.521 −0.441 −0.521 −0.782 −1.968 GPR141-Tango 0.397 0.397 −0.104 0.342 0.522 −1.851 GPR142-Tango 0.038 −0.209 0.038 −0.122 0.472 −1.472 GPR143-Tango 0.184 −0.461 −0.654 −0.317 0.019 −2.068 GPR144-Tango −0.072 0.657 −0.154 0.139 0.323 −2.505 GPR146-Tango 0.485 −1.737 −0.515 0.202 0.485 −0.515 GPR148-Tango 0.097 0.274 0.896 −0.104 0.097 −1.841 GPR149-Tango 0.280 1.345 0.830 0.652 0.757 −0.518 GPR15-Tango −0.336 −1.540 −1.211 −0.506 −0.506 −2.442 GPR150-Tango 0.621 0.387 −1.115 0.107 0.107 1.000 GPR151-Tango −0.343 0.206 −0.191 0.260 −0.191 −3.513 GPR152-Tango 0.102 −0.991 −0.509 0.090 −0.086 −2.181 GPR153-Tango 0.009 −0.036 0.025 0.108 0.357 −2.242 GPR156-Tango 1.085 0.921 0.952 0.797 1.014 −0.797 GPR157-Tango 1.104 0.837 0.508 0.541 1.126 −1.225 GPR158-Tango −0.435 −0.224 −0.010 −0.135 −0.435 0.123 GPR160-Tango −0.500 −0.392 −0.522 0.191 0.089 −2.480 GPR161-Tango −0.483 0.001 −0.377 −0.051 0.011 −2.141 GPR162-Tango −0.807 0.059 0.463 −0.062 0.372 −1.807 GPR17-Tango 0.349 0.410 0.267 0.562 0.319 −1.620 GPR171-Tango 0.807 −1.149 −0.675 0.315 0.543 −2.582 GPR173-Tango 0.274 1.159 0.097 1.159 1.528 −0.967 GPR174-Tango 0.344 −0.198 −0.331 −0.136 0.387 −0.613 GPR18-Tango 0.831 −0.037 −0.297 0.637 1.014 −1.553 GPR182-Tango 0.252 0.573 0.318 0.830 0.254 −1.511 GPR183-Tango −0.297 −0.837 −0.612 0.217 −0.161 −1.582 GPR19-Tango −0.759 −0.588 −0.881 0.163 −0.012 −2.345 GPR20-Tango 0.753 0.078 −0.801 −0.433 0.874 −1.667 GPR21-Tango 1.170 −0.862 0.000 0.379 0.888 0.202 GPR22-Tango 0.040 0.528 −0.177 0.080 0.369 −1.424 GPR25-Tango 1.217 0.314 0.491 1.376 0.598 2.463 GPR26-Tango 1.252 0.860 0.854 1.005 0.998 −1.012 GPR27-Tango 1.000 −0.495 −0.147 0.133 0.368 0.368 GPR3-Tango 0.192 0.927 0.726 0.880 0.995 −1.755 GPR31-Tango 0.184 0.329 −0.370 0.513 −0.427 −1.427 GPR32-Tango 1.550 −0.142 −0.099 1.183 1.332 −1.684 GPR34-Tango 2.058 0.943 0.871 0.808 1.498 −0.957 GPR35-Tango 0.652 0.223 0.168 0.679 1.177 −1.916 GPR37-Tango 0.452 0.533 0.510 0.835 0.810 −1.793 GPR37L1-Tango −0.029 0.192 0.019 0.403 0.425 −1.630 GPR39-Tango 1.022 −0.167 0.087 0.794 0.304 −1.678 GPR4-Tango 0.112 0.267 0.178 0.370 0.153 −2.142 GPR44-Tango 1.024 1.672 0.205 0.950 0.790 −0.182 GPR45-Tango 0.538 −0.206 −0.384 0.750 0.513 −2.121 GPR50-Tango 0.319 0.345 0.125 0.635 0.576 −1.503 GPR52-Tango 0.503 0.796 1.030 0.544 0.503 −0.020 GPR55-Tango 0.403 −0.227 −0.127 0.296 0.185 −2.061 GPR56-Tango 0.614 0.495 0.486 0.340 0.481 −1.640 GPR6-Tango 0.710 1.306 0.934 1.609 0.972 −1.198 GPR61-Tango −0.766 −0.682 −0.593 −0.071 −0.839 −2.534 GPR62-Tango −0.737 0.138 −0.737 1.202 −0.152 −0.152 GPR63-Tango −0.012 0.325 0.055 0.001 0.309 −2.288 GPR64-Tango −0.231 −0.203 −0.283 −0.203 0.099 −2.648 GPR65-Tango −0.389 −0.328 −0.220 −0.250 −0.128 −2.278 GPR68-Tango −0.540 −1.119 −1.058 −0.593 −0.546 −2.189 GPR75- Tango −0.149 −0.200 −0.258 0.114 −0.010 −2.571 GPR77-Tango 0.249 0.196 0.425 0.536 0.337 −2.123 GPR78-Tango −0.243 −0.089 −0.257 −0.124 0.006 −2.076 GPR82-Tango 0.269 0.724 0.266 0.690 0.578 −1.795 GPR83-Tango −0.345 −0.430 −0.569 0.124 0.165 −2.626 GPR84-Tango 0.174 0.629 0.191 0.706 0.312 −1.933 GPR85-Tango 0.582 0.650 0.298 0.600 0.968 −1.844 GPR87-Tango 0.155 0.242 0.157 0.396 0.537 −1.699 GPR88-Tango −0.444 −0.661 −0.794 0.432 −0.240 −2.048 GPR97-Tango 0.789 0.886 0.626 0.860 1.046 −2.716 GPRC5A-Tango −0.265 −0.603 −0.729 −0.037 −0.093 −2.340 GPRC5B-Tango −0.370 −0.215 −0.632 0.436 −0.232 −1.870 GPRC5C-Tango 0.149 −0.505 −0.488 −0.257 0.222 −2.427 GPRC5D-Tango 0.007 −0.361 −0.193 0.255 0.132 −2.299 GPRC6A-Tango −0.571 −0.583 −0.541 −0.257 −0.222 −2.582 GRM7-Tango −0.676 −0.847 −0.888 −0.589 −0.350 −2.692 GRM8-Tango 0.297 −0.696 −0.596 −0.096 −0.162 −2.567 GRPR-Tango −0.080 −0.341 −0.656 0.183 0.419 −2.854

Example 3. Human Gut Microbes Produce Compounds that Activate Aminergic Receptors

Besides the succinate receptor, the next most prevalent class of GPCRs activated by gut commensals was the aminergic receptors, which are expressed in diverse tissues and cell types and regulate a wide variety of core physiological processes ranging from neurotransmission to immunity. See, FIG. 4 . (Albuquerque et al., 2009; Beaulieu and Gainetdinov, 2011; Thurmond et al., 2008). These hits included bacterial-derived activators of the dopamine (DRDs), histamine (HRHs) and adrenergic receptor families. More than a dozen commensal supernatants activated DRD2-4 or HRH2-4, as shown in FIGS. 5A, 6A and 6B. For example, ten strains from the phylum Proteobacteria activated both DRDs and HRHs, including eight strains from the species Morganella morganii. See, FIGS. 5B, 5C, 6A and 6B. The result suggests that production of DRD and HRH agonists is a conserved feature of M. morganii.

In contrast, two strains of L. reuteri that were found to activate HRHs, while two distinct strains of L. reuteri failed to activate HRHs despite displaying similar growth kinetics. See, FIGS. 5C, 6A and 6B. One strain of Streptococcus, but not two related isolates, activated DRD2-4, while two unclassified strains of Enterobacteriaceae activated HRH1-4 and DRD2 but failed to activate other DRDs. See FIGS. 5C, 6A and 6B.

M. morganii was previously reported to produce dopamine as measured by high-performance liquid chromatography (HPLC) (Ozogul, 2004). However, it was observed that all M. morganii supernatants potently activated DRD2-4, but not DRD1 and DRD5, as shown in FIGS. 5A, 5B, 6A and 6B. In contrast, dopamine itself efficiently activated all five dopamine receptors as measured by PRESTO-Tango. See, FIG. 7A. Therefore, M. morganii might instead produce a metabolite that is structurally related to dopamine and can act as a selective ligand for DRD2-4 but not DRD1 or DRD5. See, FIG. 8A.

The inventors then examined the ability of all possible upstream and downstream metabolites in the mammalian dopamine pathway to activate DRD1-5 via PRESTO-Tango (FIGS. 8A, 8B). A variety of compounds in this pathway were found to activate various dopamine receptors; in particular, phenethylamine and tyramine showed identical activation patterns to M. morganii supernatant. See, FIGS. 8B and 8E. The concentrations of dopamine, phenethylamine, and tyramine in M. morganii supernatants were assayed. It was found that M. morganii produced only trace amounts of dopamine and no detectable tyramine. Instead M. morganii secreted significant quantities of the potent trace amine phenethylamine which, unlike dopamine and tyramine, can readily cross the blood-brain barrier. See, FIGS. 5D, 8C and 8D. (Oldendorf, 1971).

Previous reports have also suggested that M. morganii produces histamine (Ozogul, 2004). M. morganii strains indeed secreted significant amounts of histamine, as confirmed by ELISA (FIG. 5E). The histamine ELISA was conducted as follows. All strains were cultured in gut microbiota medium with or without 1% L-His for 24 hours and supernatants were collected via high-speed centrifugation. Cecal contents, colonic contents and fecal samples were collected and weighed; all samples were suspended in PBS at a ratio of 1:2 (w/v) and were homogenized by vortexing. Serum and brains were collected, weighed and suspended in PBS at a ratio of 1:2 (w/v). Brains were homogenized by passing them through a 21 G needle fifty times. All samples were centrifuged and supernatants were collected for histamine ELISA according to the manufacturer's protocol.

Similarly, two strains of L. reuteri and two strains from the Enterobacteriaceae family that activated histamine receptors also secreted histamine (FIG. 5E). Together, these data reveal that M. morganii secretes high levels of phenethylamine, which acts as a potent and selective agonist for a defined subset of the dopamine receptors, and that M. morganii and select strains of L. reuteri secrete histamine.

In mammals, phenethylamine, dopamine, and tyramine are produced via the decarboxylation of L-Phe, L-DOPA, and L-Tyr, respectively, by the aromatic L-amino acid decarboxylase (AADC; FIG. 8A) (Lovenberg, 1962). The inventors tested whether M. morganii would similarly process these three amino acids into their respective biogenic amines. A defined minimal medium (MM) lacking L-Phe, L-DOPA, L-Tyr, and L-His was used to culture M. morganii. Despite normal M. morganii growth, the inventors could not detect any production of phenethylamine, tyramine, dopamine, or histamine by liquid chromatography-mass spectrometry (LC-MS) (FIG. 5F).

However, supplementation with L-Phe or L-His led to the production of high levels of phenethylamine or histamine, and activation of DRD2-4 or HRH2-4, as shown in FIGS. 5F and 5G. In contrast, supplementation with L-DOPA or L-Tyr failed to lead to the production of dopamine or tyramine, or activation of DRDs by M. morganii supernatants despite similar bacterial growth in all conditions, as shown in FIG. 8F. This result suggests that, unlike mammalian AADC, M. morganii selectively converts L-Phe into phenethylamine and cannot efficiently convert L-DOPA or L-Tyr into dopamine or tyramine.

Example 4. Microbiota-Derived Histamine Promotes Increased Colonic Motility

Histamine is generated via decarboxylation of L-His (Tannase, 1985). Eight strains of M. morganii and two strains of L. reuteri generated histamine in vitro. Supplementation with L-His significantly increased histamine production by these strains. In contrast, two distinct strains of L. reuteri failed to produce histamine regardless of supplementation with L-His, according to the results shown in FIG. 9A. When M. morganii was cultured in multiple media, either aerobically or anaerobically, M. morganii supernatants activated DRDs and HRHs regardless of culture conditions.

To test whether M. morganii and L. reuteri can also produce histamine in vivo, germ-free mice were colonized with two distinct mock communities containing (i) 9 or 10 diverse human gut microbes or (ii) M. morganii, with or without supplementation of 1% L-His in the drinking water ad libitum to approximate the effect of an L-His-rich diet, e.g., a meat-heavy diet. 6-12 weeks-old germ-free wild-type C57Bl/6 mice of both sexes were used in all experiments. The germ-free C57Bl/6 mice were colonized via oral gavage with 200 μl of individual bacterial cultures or mixed bacterial consortia. Mock communities A and B consisted of the following taxa: Community A: Streptococcus spp; C. perfringens; B. fragilis; Erisipelotrichaceae spp; C. aerofaciens; Bacteroides UC; B. producta; Allobaculum spp and Oscillospira spp; and Community B: Bacteroides spp; P. distasonis; Peptoniphilus spp; B. ovatus; Clostridiales UC/UC; Lachnospiraceae UC/UC; C. stercoris; B. uniformis and Parabacteroides spp. All gnotobiotic mice were maintained in Techniplast P Isocages and manipulated aseptically for the duration of the experiment.

The data is shown in FIG. 9B. In addition, the inventors monocolonized mice with two strains of L. reuteri with divergent histamine production capabilities: L. reuteri C93, which produced significant histamine in vitro, and L. reuteri C88, which failed to produce histamine in vitro. Mice colonized with M. morganii or L. reuteri C93 exhibited high levels of intestinal histamine production, while mice colonized with the two mock communities or L. reuteri C88 showed nearly undetectable intestinal histamine, as shown in FIG. 9C. In addition, histamine production in M. morganii monocolonized mice was significantly increased upon supplementation with dietary L-His. See, FIG. 9C. As shown in FIG. 10A, increased histamine was detected in the serum of mice colonized with M. morganii, with or without feeding with additional L-His.

The location of M. morganii was next determined in vivo. Modified Niven's agar was used to enumerate M. morganii CFUs in gnotobiotic mice colonized with two mock communities of 9 or 10 diverse human gut microbes plus M. morganii (Mavromatis, 2002). The inventors found that M. morganii primarily inhabits the cecum and colon, and is nearly absent in the small intestine. See, FIG. 10B-10D. Previous studies in humans also indicated that M. morganii preferentially localizes in tissue- or mucus-associated niches in the colon (Eun et al., 2016).

Oral gavage with histamine has been reported to increase colon motility in rodents (Kim et al., 2011; Tyagi et al., 2009). The inventors thus hypothesized that gut microbe-derived histamine might also increase intestinal motility. The inventors assayed intestinal motility in mice colonized with two mock communities containing phylogenetically diverse human gut microbes or with M. morganii with or without administration of 1% L-His in the water. Intestinal motility was assayed by measuring fecal output as follows. Individual mice were housed in an empty container (¼ gallon) for 1 hour after which time the fecal pellets were counted and weighed. For mice fed with L-His, mice were given water containing 1% L-His ad libitum for 2 weeks before fecal output measurements.

Colonization with M. morganii led to a significant increase in fecal output, which was further increased upon supplementation with L-His. See, FIG. 9D. Similarly, mice colonized with L. reuteri C93 showed increased fecal output as compared to mice colonized with L. reuteri C88. See, FIG. 9D. These data indicate that microbiota-derived histamine can control intestinal motility and that dietary histidine can enhance these effects.

Example 5. M. morganii can Trigger ‘Phenethylamine Poisoning’ when Combined with Monoamine Oxidase Inhibition

While abundant histamine production by M. morganii was detected both in vitro and in vivo, only low levels of phenethylamine were detected in the colons of M. morganii colonized mice (FIG. 9E). A possible explanation for this observation is that biogenic amines, such as dopamine, tyramine, and phenethylamine, are rapidly degraded in the intestine by mammalian monoamine oxidases (MAOs) (Glover, 1977). To reveal the potential production of phenethylamine in vivo, the inventors treated germ-free mice or mice monocolonized with M. morganii or a Bacteroides thetaiotaomicron (B. theta) strain that does not produce DRD agonists with the irreversible MAO inhibitor (MAOI) phenelzine.

Increased phenethylamine was observed in the colons of M. morganii colonized mice by triple quadrupole MS (FIG. 9E); in contrast, colonic phenethylamine was undetectable in germ-free mice and mice colonized with B. theta and treated with MAOI (FIG. 9E). Triple quadrupole (QQQ) MS was conducted as follows. Electron Spray Ionization-Triple Quadrupole-Tandem Mass Spectrometry ESI-QQQ-MS/MS was run using Multiple Reaction Monitoring (MRM) mode. An Agilent 6490 ESI-QQQ-MS/MS instrument with a Phenomenex Kinetex 1.7 μm C18 100 Å (100×2.10 mm) column was used for quantitation and calibration. Each standard (L-Phenylalanine, Phenethylamine, Histamine, Tyramine, Dopamine) was optimized using an Agilent Mass Hunter Optimizer. A calibration curve for each standard was established using various concentrations (0-25 μM range) in triplicate. The gradient constituted 10-100% acetonitrile in ddH₂O (with 0.1% Formic Acid), then a wash step with 100% acetonitrile. The triplicate data was then subjected to linear regression analysis to produce a linear calibration curve. Processing of the experimental samples involved lyophilization and extraction with 100% MeOH (20% volume of original culture volume) before injecting samples. Sample absorbance was subjected to linear calibration to calculate concentrations.

Unlike many other irreversible MAOIs, phenelzine is still used clinically for the treatment of major depressive disorder, as well as a variety of other psychological disorders including panic, social anxiety, and post-traumatic stress disorders (Fiedorowicz, 2004). The inventors found that mice colonized with M. morganii became lethargic within days after treatment with MAOI, and more than half of all mice colonized with M. morganii died before the seventh day of treatment. See, FIG. 9F. In contrast, mice monocolonized with B. theta and treated with MAOI appeared healthy.

Morbidity and mortality after MAOI treatment correlated with elevated levels of phenethylamine in the colon, serum and brains of M. morganii monocolonized mice treated with phenelzine, as measured by QQQ MS. The results are shown in FIGS. 9G, 10E and 10F. Finally, the inventors found that cecal and colonic contents and serum from MAOI-treated M. morganii colonized mice activated DRD2. See, FIG. 9H. These data show that M. morganii-derived phenethylamine can accumulate systemically and exert dramatic biological effects in mice treated with MAOIs.

Example 6. A Unique Bacteroides Isolate Activates GPR56/AGRG1

Specific bacterial supernatants were observed to activate select orphan GPCRs (FIG. 11A). To confirm, the inventors repeated our PRESTO-Tango screening procedure using a richer culture medium (Gifu) that supports more robust growth of most of the human gut microbes in our culture collection. This modified procedure significantly expanded the number of positive hits against orphan GPCRs: 17 orphan GPCRs showed greater than four-fold activation over media only controls in response to at least one bacterial supernatant, as shown in FIG. 11B. Metabolites from a strain assigned to the species B. theta (B. theta C34) activated GPR56/AGRG1 under both culture conditions (FIG. 11C). In contrast, other strains of B. theta, including commercially available strains of B. theta and multiple other Bacteroides species, failed to activate GPR56/AGRG1 (FIG. 11D) despite similar bacterial growth (FIG. 12A).

Example 7. The Essential Amino Acid L-Phe Activates GPR56/AGRG1 and GPR97/AGRG3

Since there is no known endogenous small molecule ligand for GPR56/AGRG1 (Purcell, 2018), the inventors next attempted to identify the specific metabolite produced by B. theta C34 that activated GPR56/AGRG1. B. theta C34 supernatants were lyophilized, extracted with methanol and subjected to fractionation by reversed-phase HPLC. All resulting fractions were analyzed for activity via GPR56 PRESTO-Tango, with fraction 11 was identified as the active fraction. See, FIG. 11E.

Metabolomic assays were performed as follows. NMR spectra were taken using an Agilent 600 MHz NMR system with a cryoprobe. High-resolution MS and tandem MS (MS/MS) data were obtained using an Agilent iFunnel 6550 ESI-HRMS-QTOF (Electron Spray Ionization-High Resolution Mass Spectrometry-Quadrupole Time-of-Flight) instrument on Phenomenex Kinetex 5 μm C18 100 Å (4.6×250 mm) columns. The Agilent 1260 Infinity system with a Phenomenex Kinetex 5 μm C18 100 Å column (4.6×250 mm) or an Agilent Poroshell 120 EC-C18 2.7 μm (3.0×50 mm) column and a photodiode array (PDA) detector was used for routine sample analysis. An Agilent Prepstar HPLC system with an Agilent Polaris C18-A 5 μm (21.2×250 mm) columns were used for sample fractionation and purification.

High resolution mass spectrometry, NMR and coinjection analyses of F11 showed that the essential amino acid phenylalanine (Phe) is the primary constituent of F11 (FIGS. 11E and 12B). Finally, structural characterization using advanced Marfey's analysis confirmed that L-Phe is the likely bioactive ligand (FIG. 12C) (Bhushan and Bruckner, 2011).

The inventors next tested whether pure L-Phe or structurally related amino acids could activate GPR56/AGRG1 using GPR56-Tango. B. thetaiotaomicron strain C11 was grown in 10 mL of gut microbiota medium under anaerobic conditions at 37° C. for 24 hr. Supernatant was harvested, lyophilized and extracted with 2 mL methanol. The crude extract was then dried and fractionated using a preparative C18 HPLC system. The gradient used was 10-50% acetonitrile in water (with 0.01% trifluoroacetic acid) for 30 min, then 100% for 5 min. The fractions, which were collected every minute, were dried, resuspended in PBS buffer, and tested for bioactivity using PRESTO-Tango. The active fraction was characterized using ESI-HRMS-QTOF and NMR analyses. Stereochemistry was confirmed by advanced Marfey's analysis (FIG. 12D) (Bhushan and Bruckner, 2011).

L-Phe and, to a lesser extent, L-Tyr stereoselectively activated GPR56/AGRG1, while L-Trp and L-His, D-Phe, D-Trp, D-His and D-Tyr showed no activity. See, FIGS. 11F and 12D.

GPR56/AGRG1 is a member of the adhesion GPCR family. Adhesion GPCRs characteristically possess large extracellular domains that mediate interactions with a variety of protein ligands, such as components of the extracellular matrix (Purcell, 2018). The inventors assayed whether the extracellular domain of GPR56/AGRG1 was also required for activation by the small molecule L-Phe by constructing a truncation mutant of GPR56/AGRG1. Although this mutant is expressed normally (Kishore et al., 2016), it failed to respond to L-Phe. See, FIG. 11G. The data suggest that the extracellular domain of GPR56/AGRG1 is critical for its activation by L-Phe, a unique strain of B. theta secretes high levels of L-Phe, and L-Phe is a novel agonist of the adhesion GPCR GPR56/AGRG1.

The inventors next examined whether other orphan GPCRs might also respond to L-Phe. PRESTO-Tango screening was performed on all adhesion and orphan GPCRs stimulated with L-Phe. It was found that GPR97/AGRG3 was also activated by this compound, as shown in FIG. 13A. Upon further analysis, GPR97/AGRG3 showed greater selectivity but lower sensitivity towards L-Phe than GPR56/AGRG1—L-Phe, but not L-Tyr, L-Trp, or L-His, activated GPR97/AGRG3 (FIG. 13B). The result may explain why no significant activation of GPR97/AGRG3 by B. theta C34 supernatant was initially observed. Like GPR56/AGRG1, the extracellular domain of GPR97/AGRG3 was required for its ability to respond to L-Phe (FIG. 13C). Notably, both GPR56/AGRG1 and GPR97/AGRG3 belong to the G family of adhesion GPCRs and are closely related evolutionarily, as shown in FIG. 13D, which may explain their shared ability to detect the essential amino acid L-Phe.

Example 8. Bacterial Metabolic Exchange can Contribute to In Vivo Production of Phenethylamine

The above reductionist studies revealed that B. theta C34 produces large amounts of L-Phe while M. morganii can process L-Phe into the trace amine phenethylamine. The inventors then performed an assay to address whether these two bacteria might participate in an active metabolic exchange in vivo. The first step in investigating this hypothesis was to determine whether B. theta C34 can directly synthesize L-Phe. Using a defined minimal medium lacking L-Phe (SACC), the inventors observed that B. theta C34 could directly synthesize significant amounts of L-Phe in vitro (FIG. 14A, 14B). The inventors thus monocolonized mice fed an L-Phe deficient diet with B. theta C34 and evaluated the in vivo production of L-Phe via QQQ MS. GF mice fed with an L-Phe deficient diet exhibited reduced concentrations of L-Phe in the feces as compared to GF mice fed a conventional diet (FIG. 14C). In contrast, mice colonized with B. theta C34 and fed with an L-Phe-deficient diet exhibited significantly increased levels of L-Phe as compared to GF mice fed with an L-Phe-deficient diet.

The inventors next examined whether M. morganii would directly process B. theta C34-derived L-Phe into phenethylamine. B. theta C34 was cultured in SACC medium and then transferred B. theta supernatant to a culture of M. morganii. B. theta C34-derived L-Phe was efficiently converted into phenethylamine by M. morganii, as shown in FIG. 14D. To test whether metabolic exchange between C34 and M. morganii could contribute to in vivo production of phenethylamine, GF mice were colonized with either (i) M. morganii alone or (ii) both B. theta C34 and M. morganii. The mice were fed with a simplified diet lacking L-Phe.

These mice were then treated with phenelzine to reveal the potential production of phenethylamine. Mice colonized with M. morganii alone and fed an L-Phe deficient diet remained healthy and produced minimal phenethylamine (as measured by DRD2 activation by cecal and colonic extracts) despite MAOI treatment (FIG. 14E). In contrast, mice that were bi-colonized with C34 and M. morganii, fed an L-Phe deficient diet, and treated with MAOI became lethargic by day 4 and produced significant levels of phenethylamine as measured by DRD2 activation by cecal and colonic extracts (FIG. 14E). These results demonstrate that C34 and M. morganii can participate in an active metabolic exchange in vivo and that this exchange can contribute to the production of a bioactive trace amine that has potent effects on host physiology.

Example 9. New Ultra-Highthroughput Combinatorial GPCR Screening Technology PRESTO-Salsa Enables Simultaneous Screening of Hundreds of GPCRs in a Single Well of a 96 Well Plate

Salsa reporter cells (a stable cell line expressing a fusion protein of B-arrestin and TEV protease) were seeded into poly-D-lysine pretreated 96-well plate with 1004, DMEM+10% FBS+1% Pen/Strep. When cell density reached 90%, the cells were transfected with 100 ng of plasmid encoding each GPCR and 100 ng of a unique Salsa reporter plasmid in each well (1 well per GPCR and 314 wells total). Six hours after transfection, the cell medium was discarded followed by addition of 50 μl trypsin and 10 min incubation at 37 degrees Celsius. After digestion, an additional 504, of cell medium was added, and cells were pipetted 15 times to separate cell clusters into single cells. All transfected cells were then pooled into one 15 mL tube to generate a mixed cell library and centrifuged at 3,000 rpm for 5 min.

The supernatant was discarded, and cells were resuspended in the same volume of fresh cell medium. The cell pellet was pipetted 15 times to resuspend the cells, and then cells were reseeded at 100 μL/well into poly-D-lysine pretreated 96-well plates. Twelve hours after reseeding, the cell medium was discarded and replaced with 1004, of fresh DMEM+1% Pen/Strep, followed by stimulation with serial dilutions of the indicated GPCR ligands. Nine hours after ligand stimulation, mRNA was extracted from each well and used to prepare a amplicon DNA library for next-generation sequencing. Barcode reads after ligand stimulation divided by barcode reads without ligand stimulation were calculated to produce the fold activation for each GPCR and for each sample.

The data is shown in FIG. 16 .

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The present application describes a number of examples and embodiments of the invention. Nevertheless, it must be borne in mind that various modifications of the described examples and embodiments can be developed, while not departing from the scope and the essence of the invention in principle. With this in mind, other embodiments are included in the scope of the items listed below. At that, all the numerical ranges described herein include all the sub ranges contained therein, as well as any individual values within the scope of these ranges. All publications, patents and patent applications mentioned in this description are hereby incorporated by reference. 

1. A method for determining if a test compound modulates an activity of a first protein in vivo, comprising (a) contacting said compound with a cell comprising (i) a first nucleic acid molecule which encodes a first fusion protein comprising a first protein, a cleavage site for a protease, and a protein which activates transcription of a reporter gene in said cell, (ii) a second nucleic acid molecule which encodes a second fusion protein comprising a second protein which interacts with the first protein upon activation of the first protein and a protease or a fragment thereof capable of cleaving the protease cleavage site within the first fusion protein, and (iii) a third nucleic acid molecule which comprises a reporter gene, wherein said reporter gene is a barcode sequence operably linked to an element responsive to the protein which activates its transcription, and (b) determining the level of transcription of said barcode sequence.
 2. The method of claim 1, wherein the first nucleic acid molecule, the second nucleic acid molecule and the third nucleic acid molecule are clonally expressed to enable linkage of a specific receptor to an individual barcode.
 3. The method of claim 2, wherein the first nucleic acid molecule, the second nucleic acid molecule and the third nucleic acid molecule are clonally expressed through co-transfection.
 4. The method of claim 2, wherein the first nucleic acid molecule, the second nucleic acid molecule and the third nucleic acid molecule are clonally expressed through stable expression
 5. The method of any one of claims 1-4, wherein the barcode sequence comprises from 4 to 50 bases.
 6. The method of any one of claims 1-5, further comprising (c) concluding that the test compound activates the first protein if the level of transcription of said barcode sequence is increased in the presence of the test compound as compared to a control where no test compound is present.
 7. The method of any one of claims 1-5, further comprising (c) concluding that the test compound activates the first protein if the level of transcription of said barcode sequence is increased in the presence of the test compound as compared to an untreated cell.
 8. The method of any one of claims 1-5, further comprising (c) concluding that the test compound activates the first protein if the level of transcription of said barcode sequence is increased in the presence of the test compound as compared to a cell treated with a single dose of an agonist of the first protein in combination with an antagonist of the first protein.
 9. The method of any one of claims 1-8, wherein said first protein is a transmembrane protein.
 10. The method of any one of claims 1-9, wherein said first protein is a G-protein coupled receptor (GPCR).
 11. The method of claim 10, wherein said GPCR is a non-olfactory GPCR.
 12. The method of claim 10 or claim 11, wherein said GPCR is an orphan GPCR.
 13. The method of any one of claims 10-12, wherein the GPCR is a human GPCR.
 14. The method of any one of claims 1-13, wherein the protein which activates transcription of the reporter gene in said cell is tTA, a cas9 fusion protein, gal4/VP16, the estrogen receptor, the androgen receptor, the mineralocorticoid receptor, or the glucocorticoid receptor.
 15. The method of claim 14, wherein the cas9 fusion protein is cas9-vp64.
 16. The method of any one of claims 1-15, wherein the protein which activates transcription of the reporter gene in said cell is tTA.
 17. The method of any one of claims 1-16, wherein said second protein which interacts with the first protein upon activation of the first protein is β-arrestin, a G-protein receptor kinase (GRK), or G-alpha.
 18. The method of claim 17, wherein said second protein is β-arrestin.
 19. The method of any one of claims 1-18, wherein said protease is a Tobacco Etch Virus nuclear inclusion A protease (TEV protease).
 20. The method of any one of claims 1-19, wherein the cell is a non-adherent mammalian cell.
 21. The method of claim 20, wherein the cell is Expi 293 T cell, Jurkat, Hela T4, raji, ramos, cho-s, or thp1 cells.
 22. The method of any one of claim 1-21, wherein the level of transcription of said barcode sequence is determined by sequencing of cDNA.
 23. The method of claim 22, wherein the cDNA is produced by reverse transcription of mRNA isolated from the cell using a polydT primer.
 24. The method of any one of claims 1-23, wherein the test compound is a metabolite produced by a bacterial taxon contained within a microbiota of a subject.
 25. The method of any one of claims 1-23, wherein the test compound is a metabolite produced by a bacterial strain contained within a microbiota of a subject.
 26. The method of claim 24 or claim 25, wherein the microbiota is a gastrointestinal (GI) microbiota.
 27. The method of claim 25 or claim 26, wherein the bacterial strain is clonally arrayed and cultured in vitro.
 28. The method of any one of claims 24-27, wherein the method further comprises identifying the bacterial strain.
 29. The method of claim 28, wherein the bacterial strain is identified using 16S rRNA gene sequencing or whole genome sequencing.
 30. The method of any one of claims 25-29, wherein the subject is human.
 31. The method of any one of claims 1-30, wherein the method is conducted in a high-throughput format, comprising: (i) transfecting or transducing a plurality of cells separated into individual wells of a multi-well plate with the first, second and third nucleic acid molecules so that each transfected or transduced cell has a specific combination of the first protein and the barcode sequence; (ii) mixing the transfected cells; (iii) rearraying mixed cells into individual wells of a multi-well plate; (iv) exposing the rearrayed cell mixtures to one or more test compounds; (v) sequencing the barcodes, and (vi) determining which barcode sequences are increased in the presence of the test compound(s) as compared to a control where no test compound(s) is present.
 32. The method of any one of claims 1-30, wherein the method is conducted in a high-throughput format, comprising: (i) transfecting or transducing a plurality of cells that stably express the second nucleic acid molecule (Barr-TEV) with the first and third nucleic acid molecules (the receptor and barcode); (ii) mixing the transfected cells; (iii) rearraying mixed cells into individual wells of a multi-well plate; (iv) exposing the rearrayed cell mixtures to one or more test compounds; (v) sequencing the barcodes, and (vi) determining which barcode sequences are increased in the presence of the test compound(s) as compared to a control where no test compound(s) is present.
 33. The method of claim 32, wherein the first nucleic acid molecule encodes a GPCR, the second nucleic acid molecule encodes Barr-TEV, and the third nucleic acid molecule comprises a barcode.
 34. A method for high-throughput screening of microbiota metabolites capable of modulating the activity of a plurality of G-protein coupled receptors (GPCRs), the method comprising: a) providing a plurality of non-adherent mammalian cells, wherein each cell comprises (i) a first nucleic acid molecule encoding a first fusion protein comprising a GPCR linked to the transcription factor tTA via a cleavage site for Tobacco Etch Virus nuclear inclusion A protease (TEV protease), (ii) a second nucleic acid molecule encoding a second fusion protein comprising β-arrestin and TEV protease configured to cleave the TEV protease site on the first fusion protein, and (iii) a third nucleic acid molecule comprising a barcode sequence operably linked to a promoter specifically activated by the tTA transcription factor, wherein each barcode sequence is thus specifically linked to an individual GPCR; b) contacting the plurality of cells with one or more microbiota metabolites or one or more compounds; c) sequencing the barcodes; and d) determining which barcode sequence transcription levels are increased or decreased in the presence of the metabolites as compared to a control where no metabolites are present.
 35. The method of claim 34, wherein said GPCR is a non-olfactory GPCR.
 36. The method of claim 34 or claim 35, wherein said GPCR is an orphan GPCR.
 37. The method of any one of claims 34-36, wherein the GPCR is a human GPCR.
 38. The method of any one of claims 34-37, wherein the cell is Expi 293 T cell.
 39. The method of any one of claims 34-38, wherein the level of transcription of said barcode sequence is determined by sequencing of cDNA.
 40. The method of claim 39, wherein the cDNA is produced by reverse transcription of RNA isolated from the cell.
 41. The method of any one of claims 34-40, wherein the microbiota is a gastrointestinal (GI) microbiota.
 42. The method of any one of claims 34-41, wherein the method further comprises identifying a bacterial strain producing the specific metabolite which activates the specific GPCR.
 43. The method of claim 42, wherein the bacterial strain is identified using 16S rRNA sequencing.
 44. A method of preventing or treating monoamine oxidase inhibitor (MAOI)-induced toxicity in a subject, the method comprising administering an antibiotic effective to target a bacterial strain comprising a nucleic acid sequence encoding a phenethylamine production gene.
 45. A method of preventing or treating MAOI-induced toxicity in a subject comprising administering an antibiotic effective to target Morganella spp.
 46. A method of treating a disease or condition caused by decreased MAO activity in a subject, the method comprising administering an antibiotic effective to target an organism comprising a nucleic acid sequence encoding a phenethylamine production gene.
 47. The method of claim 46, wherein the organism is a bacterial strain.
 48. The method of any one of claim 44, 46 or 47, wherein the bacterial strain produces phenethylamine.
 49. The method of claim 48, wherein the bacterial strain secretes phenethylamine.
 50. The method of any one of claims 44 and 46-48, wherein the disease is Brunner syndrome.
 51. The method of any one of claims 44 and 46-48, wherein the condition is autism or anti-social behavior.
 52. The method of any one of claims 44 to 51, wherein the subject expresses a MAOA-L variant.
 53. The method of any one of claims 44 to 52, wherein the antibiotic is effective to target Morganella morganii.
 54. The method of any one of claims 44 to 53, wherein the antibiotic is cefepime, piperacillin, tazobactam, ceftazidime, cefotaxime, ceftibuten, meropenem, doripenem, ertapenem, a fluoroquinolone, or an aminoglycoside.
 55. A method of treating depression in a subject comprising administering a bacterial strain comprising a nucleic acid sequence encoding a phenethylamine production gene.
 56. The method of claim 55, further comprising administering an anti-depressant to the subject.
 57. The method of claim 56, wherein the anti-depressant is an MAOI.
 58. The method of claim 56 or claim 57, wherein the bacterial strain produces phenethylamine.
 59. A method for evaluating potential toxicity of a monoamine oxidase inhibitor (MAOI) in a subject, the method comprising a) obtaining a gastrointestinal microbiota sample from the subject, and b) assaying the sample for the presence of a bacterial taxon or bacterial strain comprising a phenethylamine production gene.
 60. The method of claim 59, wherein the amount of a phenethylamine producing enzyme exceeds a defined fraction of the amount of enzymes produced by the microbiota.
 61. The method of claim 59 or claim 60, wherein the gastrointestinal microbiota sample is a fecal sample.
 62. A method for evaluating potential efficacy of an MAOI in a subject, the method comprising a) taking a fecal sample or a sample from the gastrointestinal tract of the subject, and b) assaying for the presence of a bacterial strain comprising a nucleic acid sequence encoding a phenethylamine production gene.
 63. The method of any one of claims 59 to 62, further comprising assaying for the amount of the bacterial strain present in the gastrointestinal tract.
 64. The method of any one of claims 59 to 63, further comprising treating the subject with an MAOI.
 65. The method of any one of claims 59 to 64, further comprising adjusting or determining the dosage of the MAOI based on the presence and/or amount of the bacterial strain present.
 66. The method of any one of claims 59 to 65, wherein the bacterial strain is a bacterium of Morganella spp.
 67. The method of any one of claims 59 to 65, wherein the bacterial strain is Morganella morganii.
 68. A method of preventing or treating histamine-induced gastrointestinal disease in a subject, the method comprising administering one or more antibiotics effective to target a bacterial strain comprising a nucleic acid sequence encoding a histamine production gene.
 69. The method of claim 68, wherein the histamine production gene is a histidine decarboxylase.
 70. The method of claim 69, wherein the abundance of the histidine decarboxylase is higher in a patient with Crohn's disease as compared to a subject without inflammatory bowel disease.
 71. The method of claim 69, wherein the abundance of the histidine decarboxylase is higher in a patient with ulcerative colitis as compared to a subject without inflammatory bowel disease.
 72. The method of claim 68, wherein the bacterial strain is L. reuteri or a bacterium of Morganella spp.
 73. The method of any one of claims 68 to 72, wherein the gastrointestinal disease is diarrhea.
 74. A method of preventing or treating an allergy in a subject, the method comprising administering one or more antibiotics effective to target a bacterial strain comprising a nucleic acid sequence encoding a histamine production gene.
 75. The method of claim 74, wherein the bacterial strain is L. reuteri or a bacterium of Morganella spp.
 76. A method of preventing or treating asthma in a subject, the method comprising administering one or more antibiotics effective to target a bacterial strain comprising a nucleic acid sequence encoding a histamine production gene.
 77. The method of claim 76, wherein the bacterial strain is L. reuteri or a bacterium of Morganella spp.
 78. The method of any one of claims 68 to 77, wherein the antibiotic is cefepime, piperacillin, tazobactam, ceftazidime, cefotaxime, ceftibuten, meropenem, doripenem, ertapenem, a fluoroquinolone, or an aminoglycoside.
 79. The method of any one of claims 68 to 78, further comprising administering a histidine decarboxylase inhibitor.
 80. The method of claim 79, wherein the histidine decarboxylase inhibitor is rugosin D, rugosin A methyl ester, tellimagrandin II, rugosin A, pinocembrin, α-fluoromethylhistidine, brocresine, lecanoric acid, 2-hydroxy-5-carbomethoxybenzyloxyamine, and aminooxy analogs of histamine.
 81. A method for evaluating potential effectiveness of an antibiotic to treat a gastrointestinal condition or disease, allergy or asthma in a subject, the method comprising a) taking a fecal sample or a sample from the gastrointestinal tract of the subject, and b) assaying for the presence of a bacterial strain comprising a nucleic acid sequence encoding a histamine production gene.
 82. The method of claim 81, further comprising assaying for the amount of the bacterial strain present in the gastrointestinal tract.
 83. The method of claim 81 or claim 82, further comprising treating the subject with one or more antibiotics.
 84. The method of claim 83, wherein the antibiotic is cefepime, piperacillin, tazobactam, ceftazidime, cefotaxime, ceftibuten, meropenem, doripenem, ertapenem, a fluoroquinolone, or an aminoglycoside.
 85. The method of any one of claims 81 to 84, further comprising adjusting or determining the dosage of the antibiotic based on the presence and/or amount of the bacterial strain present.
 86. The method of any one of claims 81 to 85, wherein the bacterial strain is a bacterium of Morganella spp.
 87. The method of any one of claims 81 to 86, wherein the bacterial strain is Morganella morganii.
 88. A method of preventing or treating a disease or condition resulting from production of phenethylamine, the method comprising administering one or more antibiotics effective to target a bacterial strain producing L-phenylalanine.
 89. A method of preventing or treating phenylketonuria (PKU) in a subject, the method comprising administering one or more antibiotics effective to target a bacterial strain producing L-phenylalanine.
 90. A method of preventing or treating a disease or condition resulting from production of phenethylamine, the method comprising administering a Shikimate pathway inhibitor or an antagonist of aromatic L-amino acid decarboxylase.
 91. A method of preventing or treating phenylketonuria (PKU) in a subject, the method comprising administering a Shikimate pathway inhibitor or an antagonist of aromatic L-amino acid decarboxylase.
 92. The method of claim 90 or claim 91, wherein the antagonist is carbidopa, benserazide, methyldopa, 3′,4′,5,7-Tetrahydroxy-8-methoxyisoflavone (DFMD), 3-hydroxybenzylhydrazine, 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2), or α-difluoromethyl DOPA.
 93. The method of any one of claims 88 to 92, wherein the bacterial strain is B. thetaiotaomicron.
 94. The method of claim 93, wherein the bacterial strain is a strain C34 of B. thetaiotaomicron.
 95. The method of any one of claims 88 to 94, wherein the antibiotic is ampicillin, clavulanate, tazobactam, a cephamycin, ticarcillin, piperacillin, a cephalosporin, a carbapenem, clindamycin, lincomycin, chloramphenicol, a nitroimidazole, a fluoroquinolone.
 96. The method of any one of claims 88 to 94, wherein the antibiotic comprises (a) a combination of ampicillin and sulbactam, (b) a combination of ticarcillin and clavulanate, or (c) a combination of piperacillin and tazobactam.
 97. The method of any one of claims 44-54 and claims 68-96, further comprising administering a probiotic composition.
 98. The method of claim 97, wherein the probiotic composition is administered after antibiotic administration.
 99. The method of claim 97 or 98, wherein the administration of the antibiotic and the administration of the probiotic composition are repeated in a cycle.
 100. A kit for evaluating potential toxicity of an MAOI in a subject, the kit comprising a nucleic acid, antibody, or other reagent capable of binding specifically to a nucleotide or protein expressed by a bacterial strain, wherein the bacterial strain comprises a nucleic acid sequence encoding a phenethylamine production gene.
 101. The kit of claim 100, wherein the bacterial strain is a bacterium of the Morganella spp.
 102. The kit of claim 100, wherein the bacterial strain is Morganella morganii.
 103. A kit for evaluating potential effectiveness of an antibiotic to treat a gastrointestinal condition or disease, allergy or asthma in a subject, the kit comprising a nucleic acid, antibody, or other reagent capable of binding specifically to a nucleotide or protein expressed by a bacterial strain, wherein the bacterial strain comprises a nucleic acid sequence encoding a histamine production gene.
 104. The kit of claim 103, wherein the bacterial strain is L. reuteri or a bacterium of Morganella spp. 